Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,647,409 T→G 6.8% Y371D (TAT→GAT)  gor → glutathione reductase (NADPH)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,647,4090TG 6.8% 319.1 / 13.7 103Y371D (TAT→GAT) gorglutathione reductase (NADPH)
Reads supporting (aligned to +/- strand):  ref base T (57/39);  new base G (5/2);  total (62/41)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 104 total aligned reads displayed.

TGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCG  >  NC_000913/3647264‑3647556
                                                                                                                                                 |                                                                                                                                                   
tGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATaa                                                                                                                                                 <  1:1393602/150‑1 (MQ=255)
 gAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATaaa                                                                                                                                                >  2:1174775/1‑150 (MQ=255)
  aaCGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAAt                                                                                                                                               >  1:1146806/1‑150 (MQ=255)
      ccTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCctc                                                                                                                                           <  1:1344927/150‑1 (MQ=255)
      ccTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCctc                                                                                                                                           >  2:325939/1‑150 (MQ=255)
            taataaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCAc                                                                                                                                     >  1:1843291/1‑150 (MQ=255)
            taataaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTCGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCAc                                                                                                                                     <  1:483156/150‑1 (MQ=255)
              ataaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACcg                                                                                                                                   >  1:1841018/1‑150 (MQ=255)
               taaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACcgc                                                                                                                                  >  1:756635/1‑150 (MQ=255)
                aaCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGAGGATTTATACACTTTCAcctga                                                                                                                                 <  1:1666893/150‑4 (MQ=255)
                 aCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGa                                                                                                                                >  1:814431/1‑150 (MQ=255)
                 aCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGa                                                                                                                                >  2:676304/1‑150 (MQ=255)
                 aCAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGa                                                                                                                                >  1:807677/1‑150 (MQ=255)
                     gCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGta                                                                                                                            >  2:1025020/1‑150 (MQ=255)
                       cGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGtata                                                                                                                          >  1:789155/1‑150 (MQ=255)
                           tGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGAGGATTTATACACTTTCAcct                                                                                                                                   >  1:99072/1‑136 (MQ=255)
                             aGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATAccgccg                                                                                                                    >  1:411438/1‑150 (MQ=255)
                               cATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTc                                                                                                                  <  2:1587202/150‑1 (MQ=255)
                                 tCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTcac                                                                                                                <  1:325939/150‑1 (MQ=255)
                                  tttgATTAAAGCAACATTTCGACCGTGGTCTTCAGCGATACGGTGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGGGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACAGCGATGTATACCGCCGTcacc                                                                                                               <  2:847149/147‑1 (MQ=255)
                                  cTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTcacc                                                                                                               <  2:1396574/150‑1 (MQ=255)
                                   tGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTcacca                                                                                                              <  1:483989/150‑1 (MQ=255)
                                   tGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTcacca                                                                                                              >  1:1100394/1‑150 (MQ=255)
                                     gATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACt                                                                                                            >  2:970313/1‑150 (MQ=255)
                                          cAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCg                                                                                                       >  1:1969995/1‑150 (MQ=255)
                                          cAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCg                                                                                                       >  1:491169/1‑150 (MQ=255)
                                           aGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGc                                                                                                      >  1:320397/1‑150 (MQ=255)
                                            gCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGcc                                                                                                     >  2:215712/1‑150 (MQ=255)
                                            gCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGcc                                                                                                     >  1:1298349/1‑150 (MQ=255)
                                             cAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCa                                                                                                    >  1:1541083/1‑150 (MQ=255)
                                                cATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGcc                                                                                                 >  1:1980062/1‑150 (MQ=255)
                                                 aTTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCg                                                                                                <  1:676304/150‑1 (MQ=255)
                                                             gTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAg                                                                                    <  1:922743/150‑1 (MQ=255)
                                                              tCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGc                                                                                   >  1:182687/1‑150 (MQ=255)
                                                              tCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGc                                                                                   <  2:1343249/150‑1 (MQ=255)
                                                               cTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCt                                                                                  <  1:829017/150‑1 (MQ=255)
                                                                 tCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTgg                                                                                <  2:756635/150‑1 (MQ=255)
                                                                 tCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTgg                                                                                <  2:808383/150‑1 (MQ=255)
                                                                  cAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGgt                                                                               >  1:118861/1‑150 (MQ=255)
                                                                   aGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGGGAAAGTGTATAAATCCTCTTTCAGCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGTAGCTGgtg                                                                              <  2:378608/150‑1 (MQ=255)
                                                                    gCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGgtgt                                                                             <  2:1146806/150‑1 (MQ=255)
                                                                       aTCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGAAGTATGGCGACGATCAGGTGAAAGTGTATAAATCATCTTTAACCGAGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCg                                                                          >  2:1215635/1‑150 (MQ=255)
                                                                        tCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGt                                                                         <  1:989239/150‑1 (MQ=255)
                                                                         ccgcctTTTGTTACTGTTGTTTTAACGTAACCGCTGGCGCGCGTGCTGTTTGGCTACTATCTGGTTAATTTTTATAAATCCTCTTTCACCGCTATTTATACCGCCGTCACCACTCACCGCCCGCCGTGCCTCTTTACGGTGGTGTGCGtt                                                                        <  2:411438/150‑1 (MQ=255)
                                                                         ccgccgATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGtt                                                                        >  2:1432710/1‑150 (MQ=255)
                                                                         ccgccgATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGtt                                                                        <  2:1351919/150‑1 (MQ=255)
                                                                         ccgccgATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGtt                                                                        <  1:1338308/150‑1 (MQ=255)
                                                                          cgccgATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTg                                                                       >  1:905859/1‑150 (MQ=255)
                                                                          cgccgATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTg                                                                       >  2:38074/1‑150 (MQ=255)
                                                                           gccgATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTgg                                                                      >  2:1298344/1‑150 (MQ=255)
                                                                             cgATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGTCGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGAt                                                                    <  2:923148/150‑1 (MQ=255)
                                                                               aTTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGGCGTGCCGCATGAAGCTGGTGTGCGTTGGATCg                                                                  <  2:672142/150‑1 (MQ=255)
                                                                                ttGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCgg                                                                 >  2:1252615/1‑150 (MQ=255)
                                                                                 tGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGga                                                                <  1:1174775/150‑1 (MQ=255)
                                                                                   gTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaag                                                              >  1:719210/1‑150 (MQ=255)
                                                                                       tGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaagagaa                                                          >  1:166402/1‑150 (MQ=255)
                                                                                          tGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGAt                                                       >  1:1407254/1‑150 (MQ=255)
                                                                                            gTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATAc                                                                                                                         >  1:1736605/1‑82 (MQ=255)
                                                                                            gTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATAc                                                                                                                         <  2:1736605/82‑1 (MQ=255)
                                                                                              ttAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTc                                                   >  1:463280/1‑150 (MQ=255)
                                                                                                    gAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCgg                                                                 >  1:1475061/1‑130 (MQ=255)
                                                                                                    gAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCgg                                                                 <  2:1475061/130‑1 (MQ=255)
                                                                                                     aaCCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTc                                            <  2:807677/150‑1 (MQ=255)
                                                                                                     aaCCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTc                                            <  2:814431/150‑1 (MQ=255)
                                                                                                     aaCCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTc                                            <  2:178859/150‑1 (MQ=255)
                                                                                                       ccGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCAc                                          >  2:1900423/1‑150 (MQ=255)
                                                                                                          cAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGc                                       >  2:377724/1‑150 (MQ=255)
                                                                                                           aGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCa                                      >  2:980781/1‑150 (MQ=255)
                                                                                                            ggCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCAt                                     >  1:1482094/1‑150 (MQ=255)
                                                                                                              cgcgcgAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTg                                   >  2:1463367/1‑150 (MQ=255)
                                                                                                              cgcgcgAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTg                                   <  2:759029/150‑1 (MQ=255)
                                                                                                                cgcgAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGc                                 >  2:131127/1‑150 (MQ=255)
                                                                                                                     gCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTgg                            <  1:1252615/150‑1 (MQ=255)
                                                                                                                     gCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTgg                            <  2:719210/150‑1 (MQ=255)
                                                                                                                     gCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTgg                            <  1:1025020/150‑1 (MQ=255)
                                                                                                                         tATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGGATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATg                        <  2:1100394/150‑1 (MQ=255)
                                                                                                                         tATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATg                        >  2:122848/1‑150 (MQ=255)
                                                                                                                          aTGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaaga                                                             >  2:1632900/1‑112 (MQ=255)
                                                                                                                          aTGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaaga                                                             <  1:1632900/112‑1 (MQ=255)
                                                                                                                            ggCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGTCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGAc                     >  2:271254/1‑150 (MQ=255)
                                                                                                                            ggCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGAc                     >  2:604891/1‑150 (MQ=255)
                                                                                                                              cgacgaTCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGa                   >  2:1344655/1‑150 (MQ=255)
                                                                                                                              cgacgaTCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGa                   >  1:1295084/1‑150 (MQ=255)
                                                                                                                                 cgaTCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATg                        >  2:1844518/1‑142 (MQ=255)
                                                                                                                                 cgaTCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATg                        <  1:1844518/142‑1 (MQ=255)
                                                                                                                                 cgaTCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAAt                >  1:139392/1‑150 (MQ=255)
                                                                                                                                  gaTCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATg               >  1:673958/1‑150 (MQ=255)
                                                                                                                                    tCAGGTTAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGACTTGCCGCATGAAGCTGGTGTGCGTTTGATCGGAAGAGAAGATTGTCGGTATTCACGTCATTGGCTTTGGTATGGACGAAATGtt             <  1:38074/150‑1 (MQ=255)
                                                                                                                                    tCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGtt             <  2:29125/150‑1 (MQ=255)
                                                                                                                                    tCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGtt             <  2:1969995/150‑1 (MQ=255)
                                                                                                                                      cggTGAAAGAGGATTTATACACTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGc           >  1:138126/2‑150 (MQ=255)
                                                                                                                                      cggTGAAAGAGGATTTATACACTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGc           >  2:1029838/2‑150 (MQ=255)
                                                                                                                                       ggTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCa          >  1:877996/1‑150 (MQ=255)
                                                                                                                                       ggTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCa          >  1:1162337/1‑150 (MQ=255)
                                                                                                                                       ggTGAAAGAGGATTTATACACTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCa          >  2:461110/1‑150 (MQ=255)
                                                                                                                                       cgTGAAAGTGTATAAATCCTCATTCACCGCGATGTATACAGCCGTCACCACACACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCa          >  2:1491150/2‑150 (MQ=255)
                                                                                                                                         tGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAgg        >  2:1159504/1‑150 (MQ=255)
                                                                                                                                           aaaGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGc      >  1:119835/1‑150 (MQ=255)
                                                                                                                                           aaaGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGc      <  1:1774428/150‑1 (MQ=255)
                                                                                                                                               tgtATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTcg  <  1:1298344/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGACGATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCG  >  NC_000913/3647264‑3647556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑