BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,809,681	T→A	5.1%	V381D (GTT→GAT)	waaA →	KDO transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,809,681	0	T	A	5.1%	747.8 / 18.5	241	V381D (GTT→GAT)	waaA	KDO transferase
Reads supporting (aligned to +/- strand): ref base T (116/110); new base A (7/6); total (123/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

Only 100 of 245 total aligned reads displayed.

TGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGC  >  NC_000913/3809532‑3809828

tggtggGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGGTTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGt                                                                                                                                                     >  2:765343/1‑150 (MQ=255)

   tggGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGTGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTc                                                                                                                                                  >  2:438780/1‑150 (MQ=255)

   tggGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTc                                                                                                                                                  <  1:320974/150‑1 (MQ=255)

   tggGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTc                                                                                                                                                  <  2:1627354/150‑1 (MQ=255)

    gggCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTACAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTcc                                                                                                                                                 <  1:796045/150‑1 (MQ=255)

      gCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCctc                                                                                                                                               <  2:1815666/150‑1 (MQ=255)

      gCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCctc                                                                                                                                               >  1:1008296/1‑150 (MQ=255)

      gCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCctc                                                                                                                                               >  2:281262/1‑150 (MQ=255)

      gCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGAGGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCctc                                                                                                                                               <  1:1036728/150‑1 (MQ=255)

       cATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCctct                                                                                                                                              >  1:1372184/1‑150 (MQ=255)

           aTCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATTCTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGGTTCCTCTTTAc                                                                                                                                          <  1:1727734/150‑1 (MQ=255)

           aTCCGCTGGAAGCCGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCttttg                                                                                                                                          <  1:28279/150‑3 (MQ=255)

            tCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACt                                                                                                                                         >  2:1004960/1‑150 (MQ=255)

            tCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACt                                                                                                                                         <  1:1608216/150‑1 (MQ=255)

            tCCGCTGGAAGCGGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCttttgc                                                                                                                                         <  2:633754/150‑4 (MQ=255)

             ccGCTGGAAGCTGCCGCACACTCTATTCCGGTATTGATGGGGCCGCATAGTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCttttgca                                                                                                                                        <  1:536317/150‑5 (MQ=255)

              cGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGAAACCTCttttg                                                                                                                                          <  2:625206/147‑3 (MQ=255)

                cTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCAcc                                                                                                                                     <  2:611886/150‑1 (MQ=255)

                        tGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGa                                                                                                                             >  1:1156367/1‑150 (MQ=255)

                        tGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGa                                                                                                                             >  2:1872685/1‑150 (MQ=255)

                         gCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCTGGCTGATTACCGTTACCGAAGCCACTACCCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCAGAt                                                                                                                            >  1:1932044/1‑150 (MQ=255)

                            gCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTAc                                                                                                                         <  1:487041/150‑1 (MQ=255)

                            gCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTAc                                                                                                                         <  1:246783/150‑1 (MQ=255)

                            gCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTAc                                                                                                                         >  2:1057448/1‑150 (MQ=255)

                              cgatcttATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCg                                                                                                                       <  1:1699380/144‑1 (MQ=255)

                               cacGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGt                                                                                                                      <  1:404629/150‑1 (MQ=255)

                                 cggtATTCCGGGAATGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCTAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGGAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAg                                                                                                                    <  2:795377/147‑1 (MQ=255)

                                 cGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAg                                                                                                                    >  2:1700661/1‑150 (MQ=255)

                                        ccGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTAt                                                                                                             <  1:972362/150‑1 (MQ=255)

                                         cGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATg                                                                                                            <  2:328457/150‑1 (MQ=255)

                                          ggTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATgg                                                                                                           >  1:188980/1‑150 (MQ=255)

                                          ggTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATgg                                                                                                           <  2:683338/150‑1 (MQ=255)

                                          ggTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATgg                                                                                                           <  1:1588197/150‑1 (MQ=255)

                                           gTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGTGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGc                                                                                                          <  1:438780/150‑1 (MQ=255)

                                           gTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGc                                                                                                          <  2:702238/150‑1 (MQ=255)

                                           gTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGGTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGc                                                                                                          <  1:1027230/150‑1 (MQ=255)

                                                gATGGGGCCGCATACTTTTAACTTTAAAGACATTTTCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCa                                                                                                     >  2:1777508/1‑150 (MQ=255)

                                                gATGGGGCCGCATACTTTTAACTATAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCTCTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCa                                                                                                     <  2:1393094/150‑1 (MQ=255)

                                                     ggCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCg                                                                                                >  2:1562246/1‑150 (MQ=255)

                                                        cGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTg                                                                                             <  2:1383502/150‑1 (MQ=255)

                                                         gCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGa                                                                                            >  1:63268/1‑150 (MQ=255)

                                                          cATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTcc                                                                                                                                                 >  1:843983/1‑96 (MQ=255)

                                                           aTACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAg                                                                                          <  2:1097872/150‑1 (MQ=255)

                                                              cTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTAc                                                                                       >  2:1729292/1‑150 (MQ=255)

                                                                tttAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTg                                                                                     <  2:430154/150‑1 (MQ=255)

                                                                   aaCTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTAt                                                                                  <  2:1788568/150‑1 (MQ=255)

                                                                    aCTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATc                                                                                 >  1:921954/1‑150 (MQ=255)

                                                                     cTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCa                                                                                >  1:676877/1‑150 (MQ=255)

                                                                     cTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCa                                                                                <  2:1464035/150‑1 (MQ=255)

                                                                      tttAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCaa                                                                               >  1:1348122/1‑150 (MQ=255)

                                                                         aaaGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAAc                                                                            <  1:864929/150‑1 (MQ=255)

                                                                           aGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCa                                                                          <  2:1666477/150‑1 (MQ=255)

                                                                            gACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAg                                                                         <  1:34331/150‑1 (MQ=255)

                                                                              cATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAggg                                                                       >  1:482061/1‑150 (MQ=255)

                                                                                  tGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGgcgcg                                                                   <  1:820153/150‑1 (MQ=255)

                                                                                    cgcgcgGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCt                                                                 >  1:1330796/1‑150 (MQ=255)

                                                                                       gcgGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACa                                                              <  2:1142709/150‑1 (MQ=255)

                                                                                        cgGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAg                                                             >  1:1107571/1‑150 (MQ=255)

                                                                                        cgGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAg                                                             <  1:1987786/150‑1 (MQ=255)

                                                                                         ggCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGc                                                            <  1:686176/150‑1 (MQ=255)

                                                                                          gCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGACCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCg                                                           >  2:1209173/1‑150 (MQ=255)

                                                                                             ggAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCCCCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCt                                                        >  2:892246/1‑150 (MQ=255)

                                                                                                gCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCt                                                     >  1:1249797/1‑150 (MQ=255)

                                                                                                 cAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCtt                                                    >  2:1643583/1‑150 (MQ=255)

                                                                                                  aGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCCTGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCGTc                                                   >  2:1747237/1‑150 (MQ=255)

                                                                                                  aGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTc                                                   <  2:1252604/150‑1 (MQ=255)

                                                                                                   ggCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAAcc                                                                           >  1:1646677/1‑125 (MQ=255)

                                                                                                   ggCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCa                                                  >  2:1580462/1‑150 (MQ=255)

                                                                                                    gCAAGCGGGCTGGTTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCaa                                                 <  1:765343/150‑1 (MQ=255)

                                                                                                     cAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAAc                                                >  1:1412671/1‑150 (MQ=255)

                                                                                                     cAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAAc                                                >  1:1803045/1‑150 (MQ=255)

                                                                                                     cAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAAc                                                <  2:1848169/150‑1 (MQ=255)

                                                                                                         cGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAActgct                                            >  1:1230555/1‑150 (MQ=255)

                                                                                                          gggCTGATTACCGTTACCGATGCCACTAAGATTGCAAAAGAGGTTTCCTCTTTAGTCACCGACGCTGATTTCCGTATTTTCTATGGCCTTCATGCCGTTGAAGTAGTGTTTCAAAACCAGGGCGCGCTACAGGGTCTGATTCAActgctg                                           <  2:24448/150‑1 (MQ=255)

                                                                                                              tGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAAc                                       <  2:1409546/150‑1 (MQ=255)

                                                                                                               gATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAAcc                                      <  2:940032/150‑1 (MQ=255)

                                                                                                                  taccgttaccgATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTa                                   >  2:836725/1‑150 (MQ=255)

                                                                                                                   accgttaccgATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTAc                                  <  1:1189485/150‑1 (MQ=255)

                                                                                                                         accgATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCa                            <  2:848833/150‑1 (MQ=255)

                                                                                                                          ccgATGCCACTACGCTGGCAAAAGAGGTGTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCAc                           <  2:1359756/150‑1 (MQ=255)

                                                                                                                          cagctGCCAATATGCTTGCAAAAGAGGTTTGCTCTTTACTCACAGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAATCCCGTGCGCGCTACAGGGTCGTCTTCAACTGCTGGAACCTTACCTGcctc                           <  2:1932044/146‑3 (MQ=255)

                                                                                                                           cgATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCAcc                          >  2:1964900/1‑150 (MQ=255)

                                                                                                                            gATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCg                         >  2:123539/1‑150 (MQ=255)

                                                                                                                                 cACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTg                                                       <  2:376392/115‑1 (MQ=255)

                                                                                                                                 cACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTg                                                       >  1:376392/1‑115 (MQ=255)

                                                                                                                                   cTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGc                  >  1:1902381/1‑150 (MQ=255)

                                                                                                                                      cGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCAtt               >  1:941508/1‑150 (MQ=255)

                                                                                                                                      cGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCAtt               <  1:585446/150‑1 (MQ=255)

                                                                                                                                      cGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCAtt               >  2:1819166/1‑150 (MQ=255)

                                                                                                                                      cGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCAtt               >  1:824120/1‑150 (MQ=255)

                                                                                                                                        cTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGa             <  1:612461/150‑1 (MQ=255)

                                                                                                                                        cTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGa             <  1:1819166/150‑1 (MQ=255)

                                                                                                                                        cTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGa             >  1:1772691/1‑150 (MQ=255)

                                                                                                                                         tgagtaaaGAGGAAACCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAg            >  2:462572/6‑150 (MQ=255)

                                                                                                                                         tgagtaaaGAGGAAACCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAg            >  1:1794371/6‑150 (MQ=255)

                                                                                                                                           agtaaaGAGGAAACCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGgt          >  1:744498/4‑150 (MQ=255)

                                                                                                                                                agagGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTa     >  1:1518037/1‑150 (MQ=255)

                                                                                                                                                  agGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATg   >  2:488961/1‑150 (MQ=255)

                                                                                                                                                   ggTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCTCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTAttc  <  1:1091038/150‑3 (MQ=255)

                                                                                                                                                   ggTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGc  <  2:29577/150‑1 (MQ=255)

TGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGC  >  NC_000913/3809532‑3809828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`