BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,367,702	A→C	5.7%	I209S (ATC→AGC) ‡	aspA ←	aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,367,702	0	A	C	5.7%	794.3 / 29.0	244	I209S (ATC→AGC) ‡	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand): ref base A (116/114); new base C (7/7); total (123/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

CCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGG  >  NC_000913/4367555‑4367851

 cAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCTATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGc                                                                                                                                                    <  2:1308764/150‑1 (MQ=255)

    tAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGa                                                                                                                                                 <  1:301903/150‑1 (MQ=255)

    tAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGa                                                                                                                                                 >  1:1421818/1‑150 (MQ=255)

    aaaCTTCATCCAGTTTTTACACTGCCACCGCACATTACTCTATCGGAGTGATCAGACAAGTACCCATAACTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGa                                                                                                                                                 >  2:49313/2‑150 (MQ=255)

      aCTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGaaa                                                                                                                                               >  1:1108550/1‑150 (MQ=255)

      aCTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGAAGCAGTTTAGAGGTTCGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGaaa                                                                                                                                               >  2:1785817/1‑150 (MQ=255)

       cTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGTTTGCTGTTGCACCAAGGTTAACTTCCATCATCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAg                                                                                                                                              <  2:1665377/150‑1 (MQ=255)

         tCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAgaa                                                                                                                                            <  1:161494/150‑3 (MQ=255)

          cAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAgaag                                                                                                                                           <  2:1371716/150‑4 (MQ=255)

          cAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAgaag                                                                                                                                           <  2:33897/150‑4 (MQ=255)

             ccAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAg                                                                                                                                              <  2:45010/144‑1 (MQ=255)

               aGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAAt                                                                                                                                      >  2:1087567/1‑150 (MQ=255)

                  tttttCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTcc                                                                                                                                   <  1:1588480/150‑1 (MQ=255)

                         cTGCCATCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGa                                                                                                                            <  1:209504/150‑1 (MQ=255)

                          tGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAg                                                                                                                           <  1:1798981/150‑1 (MQ=255)

                           gCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAgg                                                                                                                          >  2:542966/1‑150 (MQ=255)

                            ccAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAggg                                                                                                                         <  1:1045677/150‑1 (MQ=255)

                            ccAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAggg                                                                                                                         <  1:1350137/150‑1 (MQ=255)

                            ccAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAggg                                                                                                                         <  1:1265749/150‑1 (MQ=255)

                              aGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTc                                                                                                                       >  1:1075999/1‑150 (MQ=255)

                               gCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCa                                                                                                                      >  1:1648416/1‑150 (MQ=255)

                                cGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCAt                                                                                                                     <  1:1868049/150‑1 (MQ=255)

                                  gagaGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCg                                                                                                                   >  1:197551/1‑150 (MQ=255)

                                    gagTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGt                                                                                                                 <  2:1229038/150‑1 (MQ=255)

                                         ctctTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACAGAGGGTCATCGGTACTGc                                                                                                            >  2:121395/1‑150 (MQ=255)

                                         ctctTTCGGCGTGTTCAGA‑CAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCg                                                                                                           >  1:1005621/1‑150 (MQ=255)

                                          tctTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCg                                                                                                           <  2:1866060/150‑1 (MQ=255)

                                           ctTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGt                                                                                                          >  1:1657928/1‑150 (MQ=255)

                                           ctTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGt                                                                                                          >  1:1155030/1‑150 (MQ=255)

                                            tttCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTc                                                                                                         <  2:659478/150‑1 (MQ=255)

                                             ttCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTcc                                                                                                        <  2:128040/150‑1 (MQ=255)

                                                 gCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCa                                                                                                    <  2:1416319/150‑1 (MQ=255)

                                                       tCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCTGAATTCCTGACCGAGGGTCATCGGTACTGCTTACTTCAGCTggg                                                                                              <  2:905633/150‑1 (MQ=255)

                                                       tCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTggg                                                                                              <  1:933666/150‑1 (MQ=255)

                                                       tCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTggg                                                                                              <  2:1359088/150‑1 (MQ=255)

                                                        cAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGt                                                                                             >  2:578035/1‑150 (MQ=255)

                                                        cAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGCATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGt                                                                                             >  2:1773738/1‑150 (MQ=255)

                                                              aGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGAcc                                                                                       >  1:799847/1‑150 (MQ=255)

                                                               ttagataTTTCTTTTTCACCAATGATAACTTCCAGAAGCATTTTAGTGGACCGTTGGATGGTTTTCACTTCTTTTTTCAGCAGTATGCTGAAAGCGGGTAATTCCTGACCGATGGTCATCGGTACTGCGTCCTGCTGCTGGGTACGAccc                                                                                      <  2:851898/144‑1 (MQ=255)

                                                                  ccGATTGCTGTTGCACCAAGGTTAACTTACAGCAGCAGTTGAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCAtt                                                                                   >  1:1562241/1‑150 (MQ=255)

                                                                      ttGCGGTTTCTCGAAGGTTAACTTCGAGGAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGATTTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCa                                                                               <  2:829474/150‑1 (MQ=255)

                                                                       tGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAg                                                                              <  2:197551/150‑1 (MQ=255)

                                                                       tGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAg                                                                              <  2:1182796/150‑1 (MQ=255)

                                                                       tGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAg                                                                              <  1:894231/150‑1 (MQ=255)

                                                                         cTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGa                                                                            <  2:1155030/150‑1 (MQ=255)

                                                                          tGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGAt                                                                           >  2:932845/1‑150 (MQ=255)

                                                                             tGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACt                                                                                                              >  1:875819/1‑112 (MQ=255)

                                                                                 ccAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTgg                                                                    <  2:1328108/150‑1 (MQ=255)

                                                                                      gTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTc                                                               >  2:1453296/1‑150 (MQ=255)

                                                                                       ttAACTTCCATCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGTGTACGACCGATTTTCAGGATGTCCTGGAATTCg                                                              <  2:890092/150‑1 (MQ=255)

                                                                                         aaCTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGAc                                                            >  1:427050/1‑150 (MQ=255)

                                                                                          aCTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACa                                                           >  1:621353/1‑150 (MQ=255)

                                                                                          aCTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACa                                                           >  2:1286229/1‑150 (MQ=255)

                                                                                             tCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAg                                                                              >  2:1559991/1‑128 (MQ=255)

                                                                                                agcagcagTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTa                                                     >  1:1904859/1‑150 (MQ=255)

                                                                                                 gcagcagTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTAc                                                    >  2:279014/1‑150 (MQ=255)

                                                                                                      agTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTg                                                                     <  2:1723220/128‑1 (MQ=255)

                                                                                                      agTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTg                                                                     >  1:1723220/1‑128 (MQ=255)

                                                                                                         tCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAg                                            <  2:1390477/150‑1 (MQ=255)

                                                                                                         tCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAg                                            <  2:1108550/150‑1 (MQ=255)

                                                                                                         tCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAg                                            <  1:1526789/150‑1 (MQ=255)

                                                                                                          cAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGAcc                                                                                       >  1:462526/1‑106 (MQ=255)

                                                                                                          cAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGc                                           >  2:1910200/1‑150 (MQ=255)

                                                                                                          cAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGc                                           >  1:1585076/1‑150 (MQ=255)

                                                                                                          cAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGc                                           >  1:659269/1‑150 (MQ=255)

                                                                                                          cAGCGGCACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAggg                                                                                                                         <  2:202700/72‑1 (MQ=255)

                                                                                                            gCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCt                                         <  1:1296263/150‑1 (MQ=255)

                                                                                                              ggTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTc                                       >  1:1494077/1‑150 (MQ=255)

                                                                                                              ggTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTc                                       >  1:1127750/1‑150 (MQ=255)

                                                                                                              ggTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTc                                       >  1:570332/1‑150 (MQ=255)

                                                                                                               gTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTTCGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCa                                      <  2:1270955/150‑1 (MQ=255)

                                                                                                               gTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCa                                               >  1:1839970/1‑141 (MQ=255)

                                                                                                               gTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCa                                               <  2:1839970/141‑1 (MQ=255)

                                                                                                                       gATGTTTTTCTCTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTg                              >  1:1555439/1‑150 (MQ=255)

                                                                                                                       gATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTg                              <  2:621353/150‑1 (MQ=255)

                                                                                                                        aTGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTgg                             <  1:1898726/150‑1 (MQ=255)

                                                                                                                        aTGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTgg                             <  1:355627/150‑1 (MQ=255)

                                                                                                                        aTGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTgg                             <  1:1785817/150‑1 (MQ=255)

                                                                                                                         tGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGt                            <  2:412595/150‑1 (MQ=255)

                                                                                                                         tGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGt                            <  1:1423540/150‑1 (MQ=255)

                                                                                                                            ttttCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTaa                         <  1:1252019/150‑1 (MQ=255)

                                                                                                                               tCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCg                      <  1:341202/150‑1 (MQ=255)

                                                                                                                                  cttcttcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCAt                   >  2:101710/1‑150 (MQ=255)

                                                                                                                                   ttcttcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATc                  >  1:709244/1‑150 (MQ=255)

                                                                                                                                    tcttcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCt                 >  1:1747609/1‑150 (MQ=255)

                                                                                                                                    cgcgcttTCAGCATCCTGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCt                 >  1:685923/5‑150 (MQ=255)

                                                                                                                                    cgcgcttTCAGCATCCTGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCt                 >  1:1688203/5‑150 (MQ=255)

                                                                                                                                      ttcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTAc               <  1:394838/150‑1 (MQ=255)

                                                                                                                                       tcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATc                       <  2:369952/141‑1 (MQ=255)

                                                                                                                                       tcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATc                       >  1:369952/1‑141 (MQ=255)

                                                                                                                                       tcttTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTAcc              <  2:1381647/150‑1 (MQ=255)

                                                                                                                                       gcttTCAGCATCCTGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTAcc              >  2:722912/2‑150 (MQ=255)

                                                                                                                                        cttTCAGCATCCTGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATc                  >  1:1208841/1‑145 (MQ=255)

                                                                                                                                          ttCAGCATCCTGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGc           >  1:1772032/1‑150 (MQ=255)

                                                                                                                                          ttCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGc           >  2:808771/1‑150 (MQ=255)

                                                                                                                                           tCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCt          >  1:1384031/1‑150 (MQ=255)

                                                                                                                                            cagcagGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCtt         >  2:114538/1‑150 (MQ=255)

                                                                                                                                            cagcagGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCtt         >  1:556607/1‑150 (MQ=255)

                                                                                                                                             agcagGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTa        <  2:1205999/150‑1 (MQ=255)

                                                                                                                                                   aTGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAgg  <  1:440262/150‑1 (MQ=255)

CCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGTCATCGGTACTGCGTCCTGCAGCTGGGTACGACCCATTTTCAGGATGTCCTGGAATTCGACAGCTTTACGTTCAAAGCCTTCACGCAGTTGGTTAATCGCATCTACCAGCTTAATCAGG  >  NC_000913/4367555‑4367851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`