Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,429,127 A→T 5.9% I65F (ATC→TTC) ‡ fklB → FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,1270AT 5.9% 873.8 / 25.2 268I65F (ATC→TTC) ‡fklBFKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB
Reads supporting (aligned to +/- strand):  ref base A (127/125);  new base T (5/11);  total (132/136)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 278 total aligned reads displayed.

ACGGCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGC  >  NC_000913/4428978‑4429276
                                                                                                                                                      |                                                                                                                                                      
    cATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCa                                                                                                                                                    <  1:803382/150‑1 (MQ=255)
        ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Ag                                                                                                                                               >  1:653106/1‑150 (MQ=255)
        ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Ag                                                                                                                                               <  1:1321742/150‑1 (MQ=255)
         gTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCAGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agc                                                                                                                                              >  2:1676965/1‑150 (MQ=255)
          tttGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agcg                                                                                                                                             <  2:1212480/150‑1 (MQ=255)
            tGCAGGTAGGCCAACAACTTAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGGTGTTCAGTTTGATGTGGGGCATC‑GCGCGCTGCTTGAAATCCACG‑Agcgcg                                                                                                                                           <  2:799469/150‑1 (MQ=255)
             gCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTCGTTGCAGGTATTGACGATGCGCTGGAAGGCAAACATACGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agcgcgc                                                                                                                                          >  2:1602654/1‑150 (MQ=255)
             gCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCACAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agcgcgc                                                                                                                                          >  2:939687/1‑150 (MQ=255)
             gCAGGTAGGGCAACAACTGAGTGAAGCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCT‑CGTGGATTTCACGCAgcgcg                                                                                                                                           <  1:766621/150‑1 (MQ=255)
               aGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCg                                                                                                                                        >  2:1286707/1‑150 (MQ=255)
                ggTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGa                                                                                                                                       >  1:998625/1‑150 (MQ=255)
                ggTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCT‑CGTGGATTTCACGCAgcgcgcgat                                                                                                                                       <  1:390159/150‑4 (MQ=255)
                 gTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCT‑CGTGGATTTCACGCAgcgcgcgat                                                                                                                                       <  1:1778147/150‑4 (MQ=38)
                   aGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGc                                                                                                                                    <  1:606833/150‑1 (MQ=255)
                     ggCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCT‑CGTGGATTTCACGCAGCGCG‑CGATGcac                                                                                                                                  <  1:1601084/150‑3 (MQ=37)
                      gCAACAACTGAGTGAATCTGGCCTGGAAGGGCTCCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGt                                                                                                                                 >  2:1842892/1‑150 (MQ=255)
                       caacaaCTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGtt                                                                                                                                >  2:421384/1‑150 (MQ=255)
                       caacaaCTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGtt                                                                                                                                <  1:1930680/150‑1 (MQ=255)
                       caacaaCTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCAATGGTTTCAGGTATTGCCGATTCGATGGAAGGCAAACATCCGGCAGTTACGGATGAAAAGCTGCATA‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGtt                                                                                                                                >  2:1720909/1‑150 (MQ=255)
                          caacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGCTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTtcgt                                                                                                                             <  1:570737/150‑1 (MQ=255)
                             cTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTcgtcgt                                                                                                                          >  2:258773/1‑150 (MQ=255)
                              tGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCgtcgtc                                                                                                                         <  2:125701/150‑1 (MQ=255)
                                   gAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCTGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGt                                                                                                                    >  2:596087/1‑150 (MQ=255)
                                   gAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGt                                                                                                                    >  2:1237155/1‑150 (MQ=255)
                                   gAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGt                                                                                                                    >  1:1003175/1‑150 (MQ=255)
                                     aTCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCT‑CGTGGATTTCACGCAGCGCG‑CGATGcacc                                                                                                                                 >  1:1979414/1‑132 (MQ=25)
                                     aTCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCT‑CGTGGATTTCACGCAGCGCG‑CGATGcacc                                                                                                                                 <  2:1979414/135‑4 (MQ=25)
                                       cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTcc                                                                                                                <  1:839115/150‑1 (MQ=255)
                                       cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTcc                                                                                                                <  1:1960811/150‑1 (MQ=255)
                                       cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGAAGCAGGTATTCCCGAAGCGCTGCAAGGCAAACATACGGCTGATCCGGTTGATCTCGTGCATC‑GCTCTATGCGTGAAATCCACG‑AGCGCGCCGATGCAGTTCGTCGTCAGCGTTTcc                                                                                                                >  2:1559082/1‑150 (MQ=255)
                                        tGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCa                                                                                                               >  1:1719405/1‑150 (MQ=255)
                                            cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTTTTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCg                                                                                                           <  2:220001/150‑1 (MQ=255)
                                            cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCg                                                                                                                                        >  2:373262/1‑121 (MQ=255)
                                               gAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATg                                                                                                        <  1:1211525/150‑1 (MQ=255)
                                                    gctgctGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctgc                                                                                                   >  2:1407211/1‑150 (MQ=255)
                                                          gcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                             <  1:1913465/150‑1 (MQ=255)
                                                          gcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                             >  1:1521036/1‑150 (MQ=255)
                                                          cccACAACCACTCGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCCGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                             >  2:1205088/2‑150 (MQ=255)
                                                              gAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGtgtg                                                                                         <  2:106011/150‑1 (MQ=255)
                                                              gAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGtgtg                                                                                         >  1:539342/1‑150 (MQ=255)
                                                               aaGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGa                                                                                        <  1:491189/150‑1 (MQ=255)
                                                                aGCACTGGTTGCAGGTATTGCCGATGCGCTGGAACGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGaa                                                                                       >  2:1329913/1‑150 (MQ=255)
                                                                  cACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAt                                                                                     <  2:36309/150‑1 (MQ=255)
                                                                    cTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATAc                                                                                   >  1:1568337/1‑150 (MQ=255)
                                                                      ggTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCt                                                                                 >  2:557611/1‑150 (MQ=255)
                                                                      ggTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCt                                                                                 >  1:1817743/1‑150 (MQ=255)
                                                                       gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                                <  2:1925235/150‑1 (MQ=255)
                                                                       gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                                <  1:1101060/150‑1 (MQ=255)
                                                                         tGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATAc                                                                                   >  2:1165137/1‑145 (MQ=255)
                                                                          gCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaa                                                                             <  2:682332/150‑1 (MQ=255)
                                                                           cAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaag                                                                            <  1:1909840/150‑1 (MQ=255)
                                                                             ggTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaagaa                                                                          >  1:1483148/1‑150 (MQ=255)
                                                                             ggTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaagaa                                                                          >  1:371226/1‑150 (MQ=255)
                                                                               tATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                             <  2:1501051/113‑1 (MQ=255)
                                                                                   gCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGcc                                                                    <  1:1725455/150‑1 (MQ=255)
                                                                                     cGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaa                                                                  >  1:1995693/1‑150 (MQ=255)
                                                                                     cGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaa                                                                  >  1:1330310/1‑150 (MQ=255)
                                                                                     cGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaa                                                                  <  1:1205088/150‑1 (MQ=255)
                                                                                          cgcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaag                                                             >  2:1388431/1‑150 (MQ=255)
                                                                                          cgcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaag                                                             >  1:1352948/1‑150 (MQ=255)
                                                                                          cgcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATCGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaag                                                             >  2:891218/1‑150 (MQ=255)
                                                                                           gcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaaga                                                            <  2:847847/150‑1 (MQ=255)
                                                                                            cTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaa                                                           >  1:967869/1‑150 (MQ=255)
                                                                                            cTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaa                                                           >  1:218530/1‑150 (MQ=255)
                                                                                             tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaag                                                          <  1:790221/150‑1 (MQ=255)
                                                                                              ggAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCCGGAAGAAAACGCCAAAAAAGAAgg                                                         <  1:1676965/150‑1 (MQ=255)
                                                                                                  ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                     >  2:521196/1‑150 (MQ=255)
                                                                                                    cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATA‑CCGCGCTGCGTGAAAACCACG‑AGAGCGACGATGCCCTACGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTCaa                                                   >  2:657443/1‑150 (MQ=255)
                                                                                                      aaCATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATa                                                 <  1:1847733/150‑1 (MQ=255)
                                                                                                       aCATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAg                                                >  1:1547309/1‑150 (MQ=255)
                                                                                                        cATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGc                                               <  2:967869/150‑1 (MQ=255)
                                                                                                          tCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCAc                                             <  1:1530408/150‑1 (MQ=255)
                                                                                                            cGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCg                                           >  1:1157361/1‑150 (MQ=255)
                                                                                                             ggCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGa                                          >  2:1828360/1‑150 (MQ=255)
                                                                                                                 gTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCt                                      <  1:1286707/150‑1 (MQ=255)
                                                                                                                   tCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAAc                                                                       >  1:1391063/1‑115 (MQ=255)
                                                                                                                   tCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGCAGGTGTGAATAGCACCGAATCTgg                                    >  2:614718/1‑150 (MQ=255)
                                                                                                                   tCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTgg                                    >  2:147288/1‑150 (MQ=255)
                                                                                                                     cGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGcc                                  <  1:258773/150‑1 (MQ=255)
                                                                                                                      ggTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGAGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCt                                 <  1:1237155/150‑1 (MQ=255)
                                                                                                                        ttGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGc                               <  1:421384/150‑1 (MQ=255)
                                                                                                                           aTGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAAt                            <  1:329195/150‑1 (MQ=255)
                                                                                                                             gtggtgCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTc                          <  1:596087/150‑1 (MQ=255)
                                                                                                                              tggtgCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTcc                         <  2:480762/150‑1 (MQ=255)
                                                                                                                               ggtgCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCcg                        <  1:1183094/150‑1 (MQ=255)
                                                                                                                                 ggCATCGGCGCGCT‑CGTGGATTTCACGCAGCGCG‑CGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCcgcg                      >  1:1623473/2‑150 (MQ=37)
                                                                                                                                  gCATCGGCGCGCT‑CGTGGATTTCACGCAGCGCG‑CGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGt                     >  1:86976/1‑150 (MQ=38)
                                                                                                                                  gCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGt                     <  1:939687/150‑1 (MQ=255)
                                                                                                                                   cATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTg                    <  2:1038970/150‑1 (MQ=255)
                                                                                                                                    aTC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGa                   <  2:1619500/150‑1 (MQ=255)
                                                                                                                                     tcggcgcgcT‑CGTGGATTTCACGCAGCGCG‑CGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                  <  1:1430622/147‑1 (MQ=38)
                                                                                                                                     tC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                  >  1:1038124/1‑150 (MQ=255)
                                                                                                                                     tC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                  <  2:1695951/150‑1 (MQ=255)
                                                                                                                                     tC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                  <  2:1185826/150‑1 (MQ=255)
                                                                                                                                         cgcgcTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCaa               >  1:355014/1‑150 (MQ=255)
                                                                                                                                           cgcTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAAcc             >  1:1552106/1‑150 (MQ=255)
                                                                                                                                            gcTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCa            >  1:1304701/1‑150 (MQ=255)
                                                                                                                                                cGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGt        >  1:1786877/1‑150 (MQ=255)
                                                                                                                                                  tGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGa      >  2:765984/1‑150 (MQ=255)
                                                                                                                                                     aaTCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAgg   >  2:994205/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
ACGGCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGC  >  NC_000913/4428978‑4429276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑