Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,429,129 C→T 5.8% I65I (ATC→ATT) ‡ fklB → FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,1290CT 5.8% 888.4 / 25.4 273I65I (ATC→ATT) ‡fklBFKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB
Reads supporting (aligned to +/- strand):  ref base C (127/130);  new base T (5/11);  total (132/141)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 278 total aligned reads displayed.

GCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGC  >  NC_000913/4428981‑4429278
                                                                                                                                                     |                                                                                                                                                      
 cATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCa                                                                                                                                                      <  1:803382/150‑1 (MQ=255)
  aTTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCAc                                                                                                                                                     >  1:234972/1‑150 (MQ=255)
     ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Ag                                                                                                                                                 >  1:653106/1‑150 (MQ=255)
     ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Ag                                                                                                                                                 >  1:1214893/1‑150 (MQ=255)
     ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Ag                                                                                                                                                 <  1:1321742/150‑1 (MQ=255)
     ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Ag                                                                                                                                                 <  2:1261885/150‑1 (MQ=255)
      gTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCAGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agc                                                                                                                                                >  2:1676965/1‑150 (MQ=255)
          gCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTCGTTGCAGGTATTGACGATGCGCTGGAAGGCAAACATACGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agcgcgc                                                                                                                                            >  2:1602654/1‑150 (MQ=255)
          gCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCACAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑Agcgcgc                                                                                                                                            >  2:939687/1‑150 (MQ=255)
          gCAGGTAGGGCAACAACTGAGTGAAGCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCT‑CGTGGATTTCACGCAgcgcg                                                                                                                                             <  1:766621/150‑1 (MQ=255)
           cAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGATCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGcc                                                                                                                                           >  2:860720/1‑150 (MQ=255)
               tAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCT‑CGTGGATTTCACGCAgcgcgcgatg                                                                                                                                        <  2:1224478/150‑5 (MQ=38)
                aGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGc                                                                                                                                      <  1:606833/150‑1 (MQ=255)
                  ggCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGCTGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCg                                                                                                                                    <  1:1910967/150‑1 (MQ=255)
                  ggCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCg                                                                                                                                    >  2:1363697/1‑150 (MQ=255)
                   gCAACAACTGAGTGAATCTGGCCTGGAAGGGCTCCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGt                                                                                                                                   >  2:1842892/1‑150 (MQ=255)
                    caacaaCTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGtt                                                                                                                                  >  2:421384/1‑150 (MQ=255)
                    caacaaCTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGtt                                                                                                                                  <  1:1930680/150‑1 (MQ=255)
                    caacaaCTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCAATGGTTTCAGGTATTGCCGATTCGATGGAAGGCAAACATCCGGCAGTTACGGATGAAAAGCTGCATA‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGtt                                                                                                                                  >  2:1720909/1‑150 (MQ=255)
                       caacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGCTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTtcgt                                                                                                                               <  1:570737/150‑1 (MQ=255)
                          cTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTcgtcgt                                                                                                                            >  2:258773/1‑150 (MQ=255)
                            taGTGAATCTGGCCTGGAAGGGCTGCTGCCATAAGGACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑TGCGCGCCGATGCCGTTCGTCGTCa                                                                                                                          <  2:1146437/149‑1 (MQ=255)
                               tGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCg                                                                                                                       >  1:36309/1‑150 (MQ=255)
                                gAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCTGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGt                                                                                                                      >  2:596087/1‑150 (MQ=255)
                                gAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGt                                                                                                                      >  1:1003175/1‑150 (MQ=255)
                                  aTCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCT‑CGTGGATTTCACGCAGCGCG‑CGATGcacc                                                                                                                                   <  2:1979414/135‑4 (MQ=25)
                                    cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTcc                                                                                                                  <  1:839115/150‑1 (MQ=255)
                                    cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGAAGCAGGTATTCCCGAAGCGCTGCAAGGCAAACATACGGCTGATCCGGTTGATCTCGTGCATC‑GCTCTATGCGTGAAATCCACG‑AGCGCGCCGATGCAGTTCGTCGTCAGCGTTTcc                                                                                                                  >  2:1559082/1‑150 (MQ=255)
                                        gcTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGGGCTGGAAGGCAAAGATGCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCGGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGc                                                                                                              <  1:1953502/149‑1 (MQ=255)
                                         cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTTTTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCg                                                                                                             <  2:220001/150‑1 (MQ=255)
                                         cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCg                                                                                                                                          >  2:373262/1‑121 (MQ=255)
                                         cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCg                                                                                                                                          <  1:373262/121‑1 (MQ=255)
                                         cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCg                                                                                                             >  1:1619500/1‑150 (MQ=255)
                                                 gctgctGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctgc                                                                                                     >  2:1407211/1‑150 (MQ=255)
                                                   tgctgcCAGACGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCCTC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGctgctg                                                                                                   >  2:1184892/1‑150 (MQ=255)
                                                    gctgcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGa                                                                                                  >  1:1992243/1‑150 (MQ=255)
                                                       gcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                               >  1:1528272/1‑150 (MQ=255)
                                                           gAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGtgtg                                                                                           >  1:539342/1‑150 (MQ=255)
                                                            aaGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGa                                                                                          <  1:1532352/150‑1 (MQ=255)
                                                            aaGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGa                                                                                          <  1:491189/150‑1 (MQ=255)
                                                            aaGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGa                                                                                          <  1:1772140/150‑1 (MQ=255)
                                                               cACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAt                                                                                       <  1:1582249/150‑1 (MQ=255)
                                                               cACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAt                                                                                       <  1:992069/150‑1 (MQ=255)
                                                               cACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAt                                                                                       <  2:36309/150‑1 (MQ=255)
                                                                 cTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATAc                                                                                     >  1:933246/1‑150 (MQ=255)
                                                                   ggTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCt                                                                                   >  1:1817743/1‑150 (MQ=255)
                                                                   ggTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCt                                                                                   >  2:557611/1‑150 (MQ=255)
                                                                    gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                                  <  1:41009/150‑1 (MQ=255)
                                                                    gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                                  <  2:1925235/150‑1 (MQ=255)
                                                                    gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                                  <  1:33621/150‑1 (MQ=255)
                                                                    gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                                  <  1:216108/150‑1 (MQ=255)
                                                                    gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                                  <  1:1101060/150‑1 (MQ=255)
                                                                     ttGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTgg                                                                                 <  1:1842892/150‑1 (MQ=255)
                                                                      tGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGga                                                                                <  1:1513283/150‑1 (MQ=255)
                                                                        cAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaag                                                                              <  2:1019689/150‑1 (MQ=255)
                                                                            tATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                               <  2:1501051/113‑1 (MQ=255)
                                                                               tGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGc                                                                       >  1:950060/1‑150 (MQ=255)
                                                                               tGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGc                                                                       >  2:105174/1‑150 (MQ=255)
                                                                                 ccGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGAATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCa                                                                     >  1:1721223/1‑150 (MQ=255)
                                                                                 ccGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGAATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCa                                                                     >  1:1714146/1‑150 (MQ=255)
                                                                                    aTGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaaaa                                                                  >  1:1355904/1‑150 (MQ=255)
                                                                                          tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaag                                                            <  1:790221/150‑1 (MQ=255)
                                                                                           ggAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAgg                                                           >  2:767822/1‑150 (MQ=255)
                                                                                           ggAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCCGGAAGAAAACGCCAAAAAAGAAgg                                                           <  1:1676965/150‑1 (MQ=255)
                                                                                               ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                       >  2:1237384/1‑150 (MQ=255)
                                                                                               ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                       >  2:521196/1‑150 (MQ=255)
                                                                                                gCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGa                                                      <  1:1208936/150‑1 (MQ=255)
                                                                                                 cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGaa                                                     >  1:146260/1‑150 (MQ=255)
                                                                                                 cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATA‑CCGCGCTGCGTGAAAACCACG‑AGAGCGACGATGCCCTACGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTCaa                                                     >  2:657443/1‑150 (MQ=255)
                                                                                                     cATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGc                                                 >  1:1781644/1‑150 (MQ=255)
                                                                                                     cATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGc                                                 <  2:967869/150‑1 (MQ=255)
                                                                                                         cGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCg                                             >  1:1157361/1‑150 (MQ=255)
                                                                                                          ggCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGa                                            >  2:1828360/1‑150 (MQ=255)
                                                                                                               ttCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTg                                       >  1:294103/1‑150 (MQ=255)
                                                                                                                tCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGCAGGTGTGAATAGCACCGAATCTgg                                      >  2:614718/1‑150 (MQ=255)
                                                                                                                tCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTgg                                      >  2:147288/1‑150 (MQ=255)
                                                                                                                tCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTgg                                      <  1:129862/150‑1 (MQ=255)
                                                                                                                tCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTgg                                      >  2:1193175/1‑150 (MQ=255)
                                                                                                                   ggTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCt                                   >  1:1977894/1‑150 (MQ=255)
                                                                                                                     ttGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGc                                 <  2:120974/150‑1 (MQ=255)
                                                                                                                          gtggtgCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTc                            <  1:596087/150‑1 (MQ=255)
                                                                                                                           tggtgCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTcc                           <  2:480762/150‑1 (MQ=255)
                                                                                                                               gCATCGGCGCGCT‑CGTGGATTTCACGCAGCGCG‑CGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGt                       >  1:86976/1‑150 (MQ=38)
                                                                                                                               gCATCGGCGCGCT‑CGTGGATTTCACGCAGCGCG‑CGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCT‑GAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTg                      >  1:1850926/1‑150 (MQ=37)
                                                                                                                               gCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGt                       >  1:1566564/1‑150 (MQ=255)
                                                                                                                                cATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTg                      <  2:800853/150‑1 (MQ=255)
                                                                                                                                 aTC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGa                     <  2:1619500/150‑1 (MQ=255)
                                                                                                                                  tcggcgcgcT‑CGTGGATTTCACGCAGCGCG‑CGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                    <  1:1430622/147‑1 (MQ=38)
                                                                                                                                  tC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                    <  2:746486/150‑1 (MQ=255)
                                                                                                                                  tC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                    >  1:1038124/1‑150 (MQ=255)
                                                                                                                                  tC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                    <  2:1695951/150‑1 (MQ=255)
                                                                                                                                  tC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                    <  2:1311850/150‑1 (MQ=255)
                                                                                                                                        cgcTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAAcc               >  1:1552106/1‑150 (MQ=255)
                                                                                                                                            gCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAggg           <  1:1269190/150‑1 (MQ=255)
                                                                                                                                            gCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTAAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAggg           >  1:429113/1‑150 (MQ=255)
                                                                                                                                                taaaTCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGaa       <  1:1329913/149‑1 (MQ=255)
                                                                                                                                                gAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGaa       <  1:1186072/150‑1 (MQ=255)
                                                                                                                                                  aaTCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAgg     >  1:1339831/1‑150 (MQ=255)
                                                                                                                                                  aaTCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAgg     >  2:994205/1‑150 (MQ=255)
                                                                                                                                                     ccACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCa              <  2:907780/138‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATC‑GCGCGCTGCGTGAAATCCACG‑AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGC  >  NC_000913/4428981‑4429278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑