| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 777,624 | T→C | 7.2% | intergenic (+17/‑116) | tolA → / → tolB | Tol‑Pal system protein TolA/Tol‑Pal system periplasmic protein TolB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 777,624 | 0 | T | C | 7.2% | 285.3 / 10.0 | 97 | intergenic (+17/‑116) | tolA/tolB | Tol‑Pal system protein TolA/Tol‑Pal system periplasmic protein TolB |
| Reads supporting (aligned to +/- strand): ref base T (47/43); new base C (5/2); total (52/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01 | |||||||||||
CAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTGGTT > NC_000913/777475‑777770 | cAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGtt > 2:88032/1‑150 (MQ=255)cAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTCtt > 2:867964/1‑150 (MQ=255) aaaCCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTc > 1:1951574/1‑150 (MQ=255) aaCCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCg > 2:1268553/1‑150 (MQ=255) aaCCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCg < 1:1268553/150‑1 (MQ=255) cTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGAc > 1:1393804/1‑150 (MQ=255) aGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTc > 1:724018/1‑150 (MQ=255) tGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAAc > 2:483636/1‑150 (MQ=255) tGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAAc > 2:476551/1‑150 (MQ=255) ggCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACa < 1:1240162/150‑1 (MQ=255) ggCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACa < 1:276718/150‑1 (MQ=255) ggCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACa > 1:639206/1‑150 (MQ=255) cGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATc > 1:276627/1‑150 (MQ=255) aTCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAg > 1:957520/1‑150 (MQ=255) aTCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACCGTCCGAACAGTCAACATcgc < 1:719776/150‑3 (MQ=255) cccGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGc < 2:1393804/150‑1 (MQ=255) ccGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCa > 1:638763/1‑150 (MQ=255) ccGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCa > 1:1218379/1‑150 (MQ=255) cGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCAc > 1:1884070/1‑150 (MQ=255) cACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCg > 2:588689/1‑150 (MQ=255) aGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACg > 2:1798308/1‑150 (MQ=255) ggCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACgg > 2:1588849/1‑150 (MQ=255) ggCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACgg > 1:1354082/1‑150 (MQ=255) gTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCt < 1:1668485/150‑1 (MQ=255) tGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTgg < 1:147362/150‑1 (MQ=255) cagcagCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAg < 1:740834/150‑1 (MQ=255) cagcagCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAg < 2:1354082/150‑1 (MQ=255) agcagcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGt > 2:534962/1‑150 (MQ=255) gcagcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGtt > 1:92320/1‑150 (MQ=255) agcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGtttt > 1:1858433/1‑150 (MQ=255) gcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTtg < 1:1647520/150‑1 (MQ=255) cTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTAtt > 2:1778312/1‑150 (MQ=255) ttGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTAttt < 2:639206/150‑1 (MQ=255) cGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAg < 2:276627/150‑1 (MQ=255) cGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAg < 2:1884070/150‑1 (MQ=255) cGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAg > 1:1881498/1‑150 (MQ=255) aaGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCACTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGtt < 2:50134/150‑1 (MQ=255) gATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTg > 1:389921/1‑150 (MQ=255) tCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGtt < 1:1826974/150‑1 (MQ=255) caccaAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTg < 2:971592/150‑1 (MQ=255) caccaAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTg > 1:1950082/1‑150 (MQ=255) caccaAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGGTTTGTGTATTTTAGTTTGTTAACATTCTg > 2:766069/1‑150 (MQ=255) accaAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGGCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGc < 2:1008712/150‑1 (MQ=255) ccaAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCt > 1:310576/1‑150 (MQ=255) caAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTa < 1:867964/150‑1 (MQ=255) aaGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTaa > 1:381796/1‑150 (MQ=255) aGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTAt > 1:306741/1‑150 (MQ=255) ggCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATc > 2:1217651/1‑150 (MQ=255) aGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTg < 1:1244430/150‑1 (MQ=255) tataTGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTggg > 1:735704/1‑150 (MQ=255) tataTGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTggg < 1:88032/150‑1 (MQ=255) tataTGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTggg < 2:376373/150‑1 (MQ=255) tataTGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTggg > 2:1198785/1‑150 (MQ=255) atatGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGc < 1:588689/150‑1 (MQ=255) atatGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGc < 2:724018/150‑1 (MQ=255) tatGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGcc > 2:1258496/1‑150 (MQ=255) gAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATc < 1:1653149/150‑1 (MQ=255) aaGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCg > 1:1056524/1‑150 (MQ=255) aGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCgg > 2:1477321/1‑150 (MQ=255) tgtTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTTCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTc < 2:1299561/150‑1 (MQ=255) tgtTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTc > 1:23416/1‑150 (MQ=255) gtTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTcc < 2:1218379/150‑1 (MQ=255) ttCAAAAATGGGGCATTGGACTTCAAACGGTAATTGCGATGTTGACTGTTCGGAAGGTCAACATCAGGCACGGGTTGCGAAGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAAGATTCTGCTAAATTATCGTGGGCCATCGGTCCa < 2:847105/150‑1 (MQ=255) tCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTTGTCCATCGGTcaaa > 1:847105/1‑147 (MQ=255) cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTgg > 2:1774431/1‑89 (MQ=255) cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTgg < 1:1774431/89‑1 (MQ=255) cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGcc < 1:300365/139‑1 (MQ=255) cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGcc > 2:300365/1‑139 (MQ=255) cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGa > 2:1143261/1‑150 (MQ=255) cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTCTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGa > 2:479137/1‑150 (MQ=255) ggcgcCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAggg < 1:534962/149‑1 (MQ=255) gcgcCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGga < 1:1852480/150‑1 (MQ=255) cgcCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGgag > 1:1492594/1‑150 (MQ=255) ttGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTTTTAACATTCTGATAAATTATCGTGTGCCATCTGTCCAGATAAGGGAGATatg < 2:114302/150‑1 (MQ=255) ttGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATatg < 1:1519221/150‑1 (MQ=255) ttGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATatg > 2:1533711/1‑150 (MQ=255) ggACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATatgat < 2:310576/150‑1 (MQ=255) gACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATatgatg < 1:1198785/150‑1 (MQ=255) tCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGc > 2:994801/1‑150 (MQ=255) aaaCCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAg > 1:969660/1‑150 (MQ=255) aaCCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGttt < 2:1902490/87‑1 (MQ=255) aaCCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGttt > 1:1902490/1‑87 (MQ=255) aCCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGc < 2:1056524/150‑1 (MQ=255) aCCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGc < 1:1698351/150‑1 (MQ=255) ccGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCa < 2:254744/150‑1 (MQ=255) gTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCAtt > 1:178713/1‑150 (MQ=255) aaTCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTAc > 2:786067/1‑150 (MQ=255) aTCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACg < 2:389921/150‑1 (MQ=255) tCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGa < 1:479137/150‑1 (MQ=255) gATGTTGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAAt < 1:853194/93‑1 (MQ=38) gATGTTGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAAt > 2:853194/1‑93 (MQ=38) gATGTTGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAg > 1:1061337/1‑150 (MQ=255) gATGTTGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAg > 2:270785/1‑150 (MQ=255) gATGTTGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAg > 1:1068261/1‑150 (MQ=255) ttGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAGGGGAGATATGATGAAGCAGGCATTACGAGTAGCAtt > 1:771542/1‑150 (MQ=255) gACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTg < 1:476551/150‑1 (MQ=255) gACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTg < 1:483636/150‑1 (MQ=255) gACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTg > 2:503380/1‑150 (MQ=255) tGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTGGtt < 2:690858/150‑1 (MQ=255) | CAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTGGTT > NC_000913/777475‑777770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |