Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 777,627 G→A 7.4% intergenic (+20/‑113) tolA → / → tolB Tol‑Pal system protein TolA/Tol‑Pal system periplasmic protein TolB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913777,6270GA 7.4% 288.8 / 8.0 94intergenic (+20/‑113)tolA/tolBTol‑Pal system protein TolA/Tol‑Pal system periplasmic protein TolB
Reads supporting (aligned to +/- strand):  ref base G (45/42);  new base A (5/2);  total (50/44)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTGGTTTTCTC  >  NC_000913/777480‑777775
                                                                                                                                                   |                                                                                                                                                    
cTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGAc                                                                                                                                                    >  1:1393804/1‑150 (MQ=255)
    aGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTc                                                                                                                                                >  1:724018/1‑150 (MQ=255)
       tGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAAc                                                                                                                                             >  2:483636/1‑150 (MQ=255)
       tGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAAc                                                                                                                                             >  2:476551/1‑150 (MQ=255)
        ggCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACa                                                                                                                                            <  1:1240162/150‑1 (MQ=255)
        ggCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACa                                                                                                                                            >  1:639206/1‑150 (MQ=255)
        ggCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACa                                                                                                                                            <  1:276718/150‑1 (MQ=255)
          cGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATc                                                                                                                                          >  1:276627/1‑150 (MQ=255)
            aTCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAg                                                                                                                                        >  1:957520/1‑150 (MQ=255)
            aTCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACCGTCCGAACAGTCAACATcgc                                                                                                                                        <  1:719776/150‑3 (MQ=255)
              cccGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGc                                                                                                                                      <  2:1393804/150‑1 (MQ=255)
               ccGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCa                                                                                                                                     >  1:1218379/1‑150 (MQ=255)
               ccGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCa                                                                                                                                     >  1:638763/1‑150 (MQ=255)
                cGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCAc                                                                                                                                    >  1:1884070/1‑150 (MQ=255)
                  cACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCg                                                                                                                                  >  2:588689/1‑150 (MQ=255)
                           aGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACg                                                                                                                         >  2:1798308/1‑150 (MQ=255)
                            ggCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACgg                                                                                                                        >  2:1588849/1‑150 (MQ=255)
                            ggCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACgg                                                                                                                        >  1:1354082/1‑150 (MQ=255)
                                  gTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCt                                                                                                                  <  1:1668485/150‑1 (MQ=255)
                                    tGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTgg                                                                                                                <  1:147362/150‑1 (MQ=255)
                                       cagcagCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAg                                                                                                             <  1:740834/150‑1 (MQ=255)
                                       cagcagCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAg                                                                                                             <  2:1354082/150‑1 (MQ=255)
                                        agcagcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGt                                                                                                            >  2:534962/1‑150 (MQ=255)
                                         gcagcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGtt                                                                                                           >  1:92320/1‑150 (MQ=255)
                                           agcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGtttt                                                                                                         >  1:1858433/1‑150 (MQ=255)
                                            gcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTtg                                                                                                        <  1:1647520/150‑1 (MQ=255)
                                                  cTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTAtt                                                                                                  >  2:1778312/1‑150 (MQ=255)
                                                   ttGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTAttt                                                                                                 <  2:639206/150‑1 (MQ=255)
                                                      cGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAg                                                                                              >  1:1881498/1‑150 (MQ=255)
                                                      cGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAg                                                                                              <  2:1884070/150‑1 (MQ=255)
                                                      cGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAg                                                                                              <  2:276627/150‑1 (MQ=255)
                                                        aaGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCACTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGtt                                                                                            <  2:50134/150‑1 (MQ=255)
                                                          gATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTg                                                                                          >  1:389921/1‑150 (MQ=255)
                                                            tCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGtt                                                                                        <  1:1826974/150‑1 (MQ=255)
                                                                     caccaAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTg                                                                               >  1:1950082/1‑150 (MQ=255)
                                                                     caccaAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTg                                                                               <  2:971592/150‑1 (MQ=255)
                                                                     caccaAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGGTTTGTGTATTTTAGTTTGTTAACATTCTg                                                                               >  2:766069/1‑150 (MQ=255)
                                                                      accaAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGGCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGc                                                                              <  2:1008712/150‑1 (MQ=255)
                                                                       ccaAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCt                                                                             >  1:310576/1‑150 (MQ=255)
                                                                        caAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTa                                                                            <  1:867964/150‑1 (MQ=255)
                                                                         aaGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTaa                                                                           >  1:381796/1‑150 (MQ=255)
                                                                              aGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTAt                                                                      >  1:306741/1‑150 (MQ=255)
                                                                               ggCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATc                                                                     >  2:1217651/1‑150 (MQ=255)
                                                                                  aGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTg                                                                  <  1:1244430/150‑1 (MQ=255)
                                                                                    tataTGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTggg                                                                >  2:1198785/1‑150 (MQ=255)
                                                                                    tataTGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTggg                                                                >  1:735704/1‑150 (MQ=255)
                                                                                    tataTGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTggg                                                                <  2:376373/150‑1 (MQ=255)
                                                                                    tataTGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTggg                                                                <  1:88032/150‑1 (MQ=255)
                                                                                     atatGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGc                                                               <  1:588689/150‑1 (MQ=255)
                                                                                     atatGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGc                                                               <  2:724018/150‑1 (MQ=255)
                                                                                      tatGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGcc                                                              >  2:1258496/1‑150 (MQ=255)
                                                                                         gAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATc                                                           <  1:1653149/150‑1 (MQ=255)
                                                                                          aaGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCg                                                          >  1:1056524/1‑150 (MQ=255)
                                                                                           aGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCgg                                                         >  2:1477321/1‑150 (MQ=255)
                                                                                             tgtTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTTCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTc                                                       <  2:1299561/150‑1 (MQ=255)
                                                                                             tgtTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTc                                                       >  1:23416/1‑150 (MQ=255)
                                                                                              gtTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTcc                                                      <  2:1218379/150‑1 (MQ=255)
                                                                                               ttCAAAAATGGGGCATTGGACTTCAAACGGTAATTGCGATGTTGACTGTTCGGAAGGTCAACATCAGGCACGGGTTGCGAAGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAAGATTCTGCTAAATTATCGTGGGCCATCGGTCCa                                                     <  2:847105/150‑1 (MQ=255)
                                                                                                tCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTTGTCCATCGGTcaaa                                                    >  1:847105/1‑147 (MQ=255)
                                                                                                 cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTgg                                                                                                                <  1:1774431/89‑1 (MQ=255)
                                                                                                 cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTgg                                                                                                                >  2:1774431/1‑89 (MQ=255)
                                                                                                 cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGcc                                                              <  1:300365/139‑1 (MQ=255)
                                                                                                 cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGcc                                                              >  2:300365/1‑139 (MQ=255)
                                                                                                 cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGa                                                   >  2:1143261/1‑150 (MQ=255)
                                                                                                 cAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTCTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGa                                                   >  2:479137/1‑150 (MQ=255)
                                                                                                       ggcgcCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAggg                                             <  1:534962/149‑1 (MQ=255)
                                                                                                        gcgcCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGga                                            <  1:1852480/150‑1 (MQ=255)
                                                                                                         cgcCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGgag                                           >  1:1492594/1‑150 (MQ=255)
                                                                                                              ttGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTTTTAACATTCTGATAAATTATCGTGTGCCATCTGTCCAGATAAGGGAGATatg                                      <  2:114302/150‑1 (MQ=255)
                                                                                                              ttGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATatg                                      >  2:1533711/1‑150 (MQ=255)
                                                                                                              ttGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATatg                                      <  1:1519221/150‑1 (MQ=255)
                                                                                                                ggACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATatgat                                    <  2:310576/150‑1 (MQ=255)
                                                                                                                 gACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATatgatg                                   <  1:1198785/150‑1 (MQ=255)
                                                                                                                     tCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGc                               >  2:994801/1‑150 (MQ=255)
                                                                                                                       aaaCCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAg                             >  1:969660/1‑150 (MQ=255)
                                                                                                                        aaCCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGttt                                                                                           <  2:1902490/87‑1 (MQ=255)
                                                                                                                        aaCCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGttt                                                                                           >  1:1902490/1‑87 (MQ=255)
                                                                                                                         aCCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGc                           <  2:1056524/150‑1 (MQ=255)
                                                                                                                         aCCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGc                           <  1:1698351/150‑1 (MQ=255)
                                                                                                                          ccGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCa                          <  2:254744/150‑1 (MQ=255)
                                                                                                                            gTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCAtt                        >  1:178713/1‑150 (MQ=255)
                                                                                                                              aaTCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTAc                      >  2:786067/1‑150 (MQ=255)
                                                                                                                               aTCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACg                     <  2:389921/150‑1 (MQ=255)
                                                                                                                                tCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGa                    <  1:479137/150‑1 (MQ=255)
                                                                                                                                    gATGTTGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAAt                                                                         <  1:853194/93‑1 (MQ=38)
                                                                                                                                    gATGTTGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAAt                                                                         >  2:853194/1‑93 (MQ=38)
                                                                                                                                    gATGTTGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAg                >  1:1061337/1‑150 (MQ=255)
                                                                                                                                    gATGTTGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAg                >  2:270785/1‑150 (MQ=255)
                                                                                                                                    gATGTTGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAg                >  1:1068261/1‑150 (MQ=255)
                                                                                                                                        ttGACCGTCCGAACAGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAGGGGAGATATGATGAAGCAGGCATTACGAGTAGCAtt            >  1:771542/1‑150 (MQ=255)
                                                                                                                                          gACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTg          <  1:476551/150‑1 (MQ=255)
                                                                                                                                          gACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTg          <  1:483636/150‑1 (MQ=255)
                                                                                                                                          gACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTg          >  2:503380/1‑150 (MQ=255)
                                                                                                                                             tGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTGGtt       <  2:690858/150‑1 (MQ=255)
                                                                                                                                                  ggACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTGGTTTtctc  >  2:1576705/1‑150 (MQ=255)
                                                                                                                                                  ggACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTACTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTGGTTTtctc  <  2:1951574/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAGTTTTGTGTATTTTAGTTTGTTAACATTCTGCTAAATTATCGTGGGCCATCGGTCCAGATAAGGGAGATATGATGAAGCAGGCATTACGAGTAGCATTTGGTTTTCTC  >  NC_000913/777480‑777775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: