Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 805,960 A→C 5.3% intergenic (+196/+38) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913805,9600AC 5.3% 291.8 / 6.0 93intergenic (+196/+38)ybhJ/ybhCputative hydratase YbhJ/outer membrane lipoprotein YhbC
Reads supporting (aligned to +/- strand):  ref base A (47/41);  new base C (3/2);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

CGATGCTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTATCATCT  >  NC_000913/805812‑806109
                                                                                                                                                    |                                                                                                                                                     
cgatgcTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAg                                                                                                                                                      >  2:1225705/1‑150 (MQ=255)
 gatgcTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAgg                                                                                                                                                     <  2:1505654/150‑1 (MQ=255)
  atgcTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGc                                                                                                                                                    >  2:1849016/1‑150 (MQ=255)
  atgcTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGc                                                                                                                                                    <  2:87510/150‑1 (MQ=255)
   tgcTTGCGCATCTTATCCGACCTACGAATCGCTTCGTATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCg                                                                                                                                                   <  2:542860/150‑1 (MQ=255)
   tgcTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCg                                                                                                                                                   <  1:1062488/150‑1 (MQ=255)
   tgcTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCg                                                                                                                                                   <  1:1069406/150‑1 (MQ=255)
     cTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGtt                                                                                                                                                 <  2:1431135/150‑1 (MQ=255)
      ttGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGttt                                                                                                                                                >  1:474957/1‑150 (MQ=255)
              cTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCa                                                                                                                                        <  2:738615/150‑1 (MQ=255)
              cTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCAGACCTACAAATCGCATCGAACCGTAGGCCGGATGCGGCGTAAAcgcctta                                                                                                                                        >  1:645559/1‑147 (MQ=37)
                 aTCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                     <  2:1361385/150‑1 (MQ=255)
                   ccGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                   <  2:421873/150‑1 (MQ=255)
                    cGACCTACGAATCGCATCGAATCTGTAGCCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                  <  1:1849016/150‑1 (MQ=255)
                      aCCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                                                                                                                <  1:653531/150‑3 (MQ=37)
                      aCCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                                                                                                                <  2:645559/150‑3 (MQ=37)
                          aCGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGTCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAg                                                                                                                            >  2:123946/1‑150 (MQ=255)
                                 gCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTg                                                                                                                     >  2:1408811/1‑150 (MQ=255)
                                  cATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGc                                                                                                                    >  1:1826821/1‑150 (MQ=255)
                                    tCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGctc                                                                                                                  <  2:1422427/150‑1 (MQ=255)
                                        aTCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGAAGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTAc                                                                                                              <  1:1007546/150‑1 (MQ=255)
                                            gTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTActtct                                                                                                          >  2:892918/1‑150 (MQ=255)
                                            gTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTActtct                                                                                                          >  2:199234/1‑150 (MQ=255)
                                             tAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTActtctt                                                                                                         <  1:123946/150‑1 (MQ=255)
                                               ggCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGTGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCg                                                                                                       >  1:1470487/1‑150 (MQ=255)
                                               ggCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCg                                                                                                       >  1:924700/1‑150 (MQ=255)
                                                gCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGc                                                                                                      >  2:1379976/1‑150 (MQ=255)
                                                 ccAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGcc                                                                                                     >  1:168426/1‑150 (MQ=255)
                                                      tAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGc                                                                                                <  2:1218313/150‑1 (MQ=255)
                                                      tAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGc                                                                                                <  1:892918/150‑1 (MQ=255)
                                                      tAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGc                                                                                                <  1:1225705/150‑1 (MQ=255)
                                                       aaGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCa                                                                                               >  1:1577338/1‑150 (MQ=255)
                                                         ggCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAAc                                                                                             <  2:169876/150‑1 (MQ=255)
                                                          gCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAAcc                                                                                            >  1:1806505/1‑150 (MQ=255)
                                                          gCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAAcc                                                                                            >  2:1548900/1‑150 (MQ=255)
                                                           cATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCa                                                                                           >  1:775785/1‑150 (MQ=255)
                                                                gcatcgcaTCCGACAATAAGTGCCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTta                                                                                      >  1:663705/6‑150 (MQ=25)
                                                                 cGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTtac                                                                                     <  2:474957/150‑1 (MQ=255)
                                                                  gcagcaTCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTtact                                                                                    >  2:1747814/1‑150 (MQ=255)
                                                                    agcaTCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTtactac                                                                                  <  1:1233776/150‑1 (MQ=255)
                                                                       aTCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCa                                                                               >  2:439574/1‑150 (MQ=255)
                                                                          cGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCAcgc                                                                            >  1:134277/1‑150 (MQ=255)
                                                                           ggCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCAcgcc                                                                           >  2:1737717/1‑150 (MQ=255)
                                                                           ggCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCAcgcc                                                                           >  1:1887654/1‑150 (MQ=255)
                                                                              aCTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCcgcg                                                                        <  1:143562/150‑1 (MQ=255)
                                                                                  aTTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTa                                                                    >  1:128091/1‑150 (MQ=255)
                                                                                     gTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTg                                                                 <  1:1714906/150‑1 (MQ=255)
                                                                                     gTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTCTACTACCCACGCCGCGGTTATTg                                                                 >  2:1559784/1‑150 (MQ=255)
                                                                                       cGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTa                                                               >  1:1721753/1‑150 (MQ=255)
                                                                                        ggATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTAt                                                              >  1:354976/1‑150 (MQ=255)
                                                                                        ggATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTAt                                                              <  1:740012/150‑1 (MQ=255)
                                                                                             cgatgcTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCca                                                         >  2:1073294/1‑150 (MQ=255)
                                                                                             cgatgcTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCca                                                         <  2:1171053/150‑1 (MQ=255)
                                                                                               atgcTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCcaca                                                       >  1:709644/1‑150 (MQ=255)
                                                                                                    tGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCgg                                                  >  1:418707/1‑150 (MQ=255)
                                                                                                       gcATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTA‑CCACGCCGCGGTTATTGTATTCCCACATGCGGTTGt                                              <  1:1408811/150‑1 (MQ=255)
                                                                                                         aTCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGta                                             >  2:1746344/1‑150 (MQ=255)
                                                                                                            ttATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGtagtt                                          <  2:924700/150‑1 (MQ=255)
                                                                                                              aTCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGtagttag                                        <  1:1739023/150‑1 (MQ=255)
                                                                                                              aTCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGtagttag                                        <  2:817271/150‑1 (MQ=255)
                                                                                                              aTCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGtagttag                                        <  1:1143744/150‑1 (MQ=255)
                                                                                                                ccGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAgtg                                      >  1:1147837/1‑150 (MQ=255)
                                                                                                                  gACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTc                                    >  1:997330/1‑150 (MQ=255)
                                                                                                                   aCCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCa                                   <  2:1806505/150‑1 (MQ=255)
                                                                                                                      tACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTc                                >  2:1304862/1‑150 (MQ=255)
                                                                                                                       aCAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCa                               >  2:1226203/1‑150 (MQ=255)
                                                                                                                       aCAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCa                               >  1:62026/1‑150 (MQ=255)
                                                                                                                           aTCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGAtt                           >  1:1786209/1‑150 (MQ=255)
                                                                                                                              gCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTgcg                        >  1:1681137/1‑150 (MQ=255)
                                                                                                                               cATCGAACCGTAGGCCGGATAAGGTGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTgcgc                       <  2:1470487/150‑1 (MQ=255)
                                                                                                                               cATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTgcgc                       <  2:1887654/150‑1 (MQ=255)
                                                                                                                               cATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTgcgc                       <  1:610643/150‑1 (MQ=255)
                                                                                                                                  cGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGt                    >  2:285399/1‑150 (MQ=255)
                                                                                                                                   gAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTa                   >  2:281715/1‑150 (MQ=255)
                                                                                                                                   gAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTa                   >  2:995982/1‑150 (MQ=255)
                                                                                                                                   gAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTa                   >  1:1294478/1‑150 (MQ=255)
                                                                                                                                      ctatttgCCGGATGCGGCGTAAACGCCTTATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTAgtt                >  1:1468416/7‑150 (MQ=38)
                                                                                                                                      ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTAttt                >  1:254157/1‑150 (MQ=255)
                                                                                                                                      ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTAttt                >  1:20270/1‑150 (MQ=255)
                                                                                                                                      ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTAttt                <  1:1747814/150‑1 (MQ=255)
                                                                                                                                      ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTAttt                <  1:1737717/150‑1 (MQ=255)
                                                                                                                                        gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTtcg              <  1:1304862/150‑1 (MQ=255)
                                                                                                                                         tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTtcgt             >  1:1436602/1‑150 (MQ=255)
                                                                                                                                           tgCCGGATGCGGCGTAAACGCCTTATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCCGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTtcgtcg           >  1:1646584/2‑150 (MQ=255)
                                                                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTtcgtcg           <  2:1618253/150‑1 (MQ=255)
                                                                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTtcgtcg           <  1:579788/150‑1 (MQ=255)
                                                                                                                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTcgtcgt          <  2:332459/150‑1 (MQ=255)
                                                                                                                                              cGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTa        <  1:199234/150‑1 (MQ=255)
                                                                                                                                                gATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatc      <  2:1681137/150‑1 (MQ=255)
                                                                                                                                                 aTAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatca     <  2:920642/150‑1 (MQ=255)
                                                                                                                                                  tAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatcat    >  1:1029159/1‑150 (MQ=255)
                                                                                                                                                  tAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatcat    >  2:825947/1‑150 (MQ=255)
                                                                                                                                                  tAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatcat    >  1:1643974/1‑150 (MQ=255)
                                                                                                                                                   aaGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatcatc   >  1:737012/1‑150 (MQ=255)
                                                                                                                                                    aGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTATCATCt  >  2:701708/1‑150 (MQ=255)
                                                                                                                                                    aGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTATCATCt  >  1:1670596/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGATGCTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTATCATCT  >  NC_000913/805812‑806109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑