Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 805,966 T→A 5.7% intergenic (+202/+32) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913805,9660TA 5.7% 284.1 / 5.4 88intergenic (+202/+32)ybhJ/ybhCputative hydratase YbhJ/outer membrane lipoprotein YhbC
Reads supporting (aligned to +/- strand):  ref base T (47/36);  new base A (3/2);  total (50/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

CTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTATCATCTAC  >  NC_000913/805817‑806111
                                                                                                                                                     |                                                                                                                                                 
cTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGtt                                                                                                                                                   <  2:1431135/150‑1 (MQ=255)
 ttGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGttt                                                                                                                                                  >  1:474957/1‑150 (MQ=255)
         cTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCa                                                                                                                                          <  2:738615/150‑1 (MQ=255)
         cTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCAGACCTACAAATCGCATCGAACCGTAGGCCGGATGCGGCGTAAAcgcctta                                                                                                                                          >  1:645559/1‑147 (MQ=37)
            aTCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                       <  2:1361385/150‑1 (MQ=255)
              ccGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                     <  2:421873/150‑1 (MQ=255)
               cGACCTACGAATCGCATCGAATCTGTAGCCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                    <  1:1849016/150‑1 (MQ=255)
                 aCCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                                                                                                                  <  1:653531/150‑3 (MQ=37)
                 aCCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                                                                                                                  <  2:645559/150‑3 (MQ=37)
                     aCGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGTCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAg                                                                                                                              >  2:123946/1‑150 (MQ=255)
                            gCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTg                                                                                                                       >  2:1408811/1‑150 (MQ=255)
                             cATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGc                                                                                                                      >  1:1826821/1‑150 (MQ=255)
                               tCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGctc                                                                                                                    <  2:1422427/150‑1 (MQ=255)
                                   aTCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGAAGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTAc                                                                                                                <  1:1007546/150‑1 (MQ=255)
                                       gTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTActtct                                                                                                            >  2:892918/1‑150 (MQ=255)
                                       gTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTActtct                                                                                                            >  2:199234/1‑150 (MQ=255)
                                        tAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTActtctt                                                                                                           <  1:123946/150‑1 (MQ=255)
                                          ggCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGTGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCg                                                                                                         >  1:1470487/1‑150 (MQ=255)
                                          ggCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCg                                                                                                         >  1:924700/1‑150 (MQ=255)
                                           gCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGc                                                                                                        >  2:1379976/1‑150 (MQ=255)
                                            ccAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGcc                                                                                                       >  1:168426/1‑150 (MQ=255)
                                                 tAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGc                                                                                                  <  1:892918/150‑1 (MQ=255)
                                                 tAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGc                                                                                                  <  2:1218313/150‑1 (MQ=255)
                                                 tAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGc                                                                                                  <  1:1225705/150‑1 (MQ=255)
                                                  aaGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCa                                                                                                 >  1:1577338/1‑150 (MQ=255)
                                                    ggCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAAc                                                                                               <  2:169876/150‑1 (MQ=255)
                                                     gCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAAcc                                                                                              >  1:1806505/1‑150 (MQ=255)
                                                     gCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAAcc                                                                                              >  2:1548900/1‑150 (MQ=255)
                                                      cATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCa                                                                                             >  1:775785/1‑150 (MQ=255)
                                                           gcatcgcaTCCGACAATAAGTGCCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTta                                                                                        >  1:663705/6‑150 (MQ=25)
                                                            cGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTtac                                                                                       <  2:474957/150‑1 (MQ=255)
                                                             gcagcaTCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTtact                                                                                      >  2:1747814/1‑150 (MQ=255)
                                                               agcaTCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTtactac                                                                                    <  1:1233776/150‑1 (MQ=255)
                                                                  aTCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCa                                                                                 >  2:439574/1‑150 (MQ=255)
                                                                     cGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCAcgc                                                                              >  1:134277/1‑150 (MQ=255)
                                                                      ggCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCAcgcc                                                                             >  2:1737717/1‑150 (MQ=255)
                                                                      ggCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCAcgcc                                                                             >  1:1887654/1‑150 (MQ=255)
                                                                         aCTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCcgcg                                                                          <  1:143562/150‑1 (MQ=255)
                                                                             aTTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTa                                                                      >  1:128091/1‑150 (MQ=255)
                                                                                gTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTg                                                                   <  1:1714906/150‑1 (MQ=255)
                                                                                gTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTCTACTACCCACGCCGCGGTTATTg                                                                   >  2:1559784/1‑150 (MQ=255)
                                                                                  cGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTa                                                                 >  1:1721753/1‑150 (MQ=255)
                                                                                   ggATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTAt                                                                >  1:354976/1‑150 (MQ=255)
                                                                                   ggATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTAt                                                                <  1:740012/150‑1 (MQ=255)
                                                                                        cgatgcTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCca                                                           <  2:1171053/150‑1 (MQ=255)
                                                                                        cgatgcTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCca                                                           >  2:1073294/1‑150 (MQ=255)
                                                                                          atgcTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCcaca                                                         >  1:709644/1‑150 (MQ=255)
                                                                                               tGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCgg                                                    >  1:418707/1‑150 (MQ=255)
                                                                                                  gcATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTA‑CCACGCCGCGGTTATTGTATTCCCACATGCGGTTGt                                                <  1:1408811/150‑1 (MQ=255)
                                                                                                    aTCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGta                                               >  2:1746344/1‑150 (MQ=255)
                                                                                                       ttATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGtagtt                                            <  2:924700/150‑1 (MQ=255)
                                                                                                         aTCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGtagttag                                          <  1:1739023/150‑1 (MQ=255)
                                                                                                         aTCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGtagttag                                          <  1:1143744/150‑1 (MQ=255)
                                                                                                         aTCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGtagttag                                          <  2:817271/150‑1 (MQ=255)
                                                                                                           ccGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAgtg                                        >  1:1147837/1‑150 (MQ=255)
                                                                                                             gACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTc                                      >  1:997330/1‑150 (MQ=255)
                                                                                                              aCCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCa                                     <  2:1806505/150‑1 (MQ=255)
                                                                                                                 tACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTc                                  >  2:1304862/1‑150 (MQ=255)
                                                                                                                  aCAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCa                                 >  2:1226203/1‑150 (MQ=255)
                                                                                                                  aCAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCa                                 >  1:62026/1‑150 (MQ=255)
                                                                                                                      aTCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGAtt                             >  1:1786209/1‑150 (MQ=255)
                                                                                                                         gCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTgcg                          >  1:1681137/1‑150 (MQ=255)
                                                                                                                          cATCGAACCGTAGGCCGGATAAGGTGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTgcgc                         <  2:1470487/150‑1 (MQ=255)
                                                                                                                          cATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTgcgc                         <  2:1887654/150‑1 (MQ=255)
                                                                                                                          cATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTgcgc                         <  1:610643/150‑1 (MQ=255)
                                                                                                                             cGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGt                      >  2:285399/1‑150 (MQ=255)
                                                                                                                              gAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTa                     >  2:995982/1‑150 (MQ=255)
                                                                                                                              gAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTa                     >  2:281715/1‑150 (MQ=255)
                                                                                                                              gAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTa                     >  1:1294478/1‑150 (MQ=255)
                                                                                                                                 ctatttgCCGGATGCGGCGTAAACGCCTTATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTAgtt                  >  1:1468416/7‑150 (MQ=38)
                                                                                                                                 ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTAttt                  >  1:254157/1‑150 (MQ=255)
                                                                                                                                 ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTAttt                  >  1:20270/1‑150 (MQ=255)
                                                                                                                                 ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTAttt                  <  1:1747814/150‑1 (MQ=255)
                                                                                                                                 ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTAttt                  <  1:1737717/150‑1 (MQ=255)
                                                                                                                                   gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTtcg                <  1:1304862/150‑1 (MQ=255)
                                                                                                                                    tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTtcgt               >  1:1436602/1‑150 (MQ=255)
                                                                                                                                      tgCCGGATGCGGCGTAAACGCCTTATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCCGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTtcgtcg             >  1:1646584/2‑150 (MQ=255)
                                                                                                                                      ggCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTtcgtcg             <  2:1618253/150‑1 (MQ=255)
                                                                                                                                      ggCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTtcgtcg             <  1:579788/150‑1 (MQ=255)
                                                                                                                                       gCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTcgtcgt            <  2:332459/150‑1 (MQ=255)
                                                                                                                                         cGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTa          <  1:199234/150‑1 (MQ=255)
                                                                                                                                           gATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatc        <  2:1681137/150‑1 (MQ=255)
                                                                                                                                            aTAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatca       <  2:920642/150‑1 (MQ=255)
                                                                                                                                             tAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatcat      >  1:1643974/1‑150 (MQ=255)
                                                                                                                                             tAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatcat      >  2:825947/1‑150 (MQ=255)
                                                                                                                                             tAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatcat      >  1:1029159/1‑150 (MQ=255)
                                                                                                                                              aaGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTatcatc     >  1:737012/1‑150 (MQ=255)
                                                                                                                                               aGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTATCATCt    >  1:1670596/1‑150 (MQ=255)
                                                                                                                                               aGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTATCATCt    >  2:701708/1‑150 (MQ=255)
                                                                                                                                                 gCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTATCATCTAc  <  1:1257519/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CTTGCGCATCTTATCCGACCTACGAATCGCATCGAATCTGTAGGCCAGATAAGGCATTTTCGCAGCATCCGGCACTTATTGTCGGATGCGATGCTTGCGCATCTTATCCGACCTACAAATCGCATCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAATAGTTAATTGCTCTTACTTCTTCGCCTCTGCAACCACTTTACTACCCACGCCGCGGTTATTGTATTCCCACATGCGGTTGTAGTTAGTGTCATTCAGATTGCGCTGTATTTCGTCGTTATCATCTAC  >  NC_000913/805817‑806111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑