Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,349,053 A→T 7.9% I352N (ATT→AAT)  yciW ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,349,0530AT 7.9% 269.8 / 10.8 89I352N (ATT→AAT) yciWputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (42/40);  new base T (3/4);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

TGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCG  >  NC_000913/1348905‑1349202
                                                                                                                                                    |                                                                                                                                                     
tGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATtaat                                                                                                                                                      >  1:1487291/1‑150 (MQ=255)
tGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATtaat                                                                                                                                                      <  1:1447626/150‑1 (MQ=255)
          ttGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTATTAATCTGctgg                                                                                                                                            <  1:568976/150‑4 (MQ=255)
          ttGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTATTAATCTGctgg                                                                                                                                            <  1:456292/150‑4 (MQ=255)
          ttGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGtt                                                                                                                                            <  1:1757333/150‑1 (MQ=255)
           tGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTATTAATCTGctggt                                                                                                                                           <  1:7164/150‑5 (MQ=255)
            gTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTATTAATCTGctggta                                                                                                                                          <  1:1703871/150‑6 (MQ=255)
            gTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTtga                                                                                                                                          >  2:1328885/1‑150 (MQ=255)
            gTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTtga                                                                                                                                          >  1:851019/1‑150 (MQ=255)
            gTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTtga                                                                                                                                          <  2:1487291/150‑1 (MQ=255)
             tCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTtgat                                                                                                                                         <  2:1637273/150‑1 (MQ=255)
              cTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTtgatg                                                                                                                                        <  2:1381290/150‑1 (MQ=255)
               tATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTtgatga                                                                                                                                       >  2:424768/1‑150 (MQ=255)
                  tttGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGAttt                                                                                                                                    >  2:1210139/1‑150 (MQ=255)
                    tggtggCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGAttttt                                                                                                                                  >  2:1680581/1‑150 (MQ=255)
                     ggtggCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTc                                                                                                                                 >  2:1176359/1‑150 (MQ=255)
                      gtggCCATTAGAGCGTCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTATCACCCAACTCGATTTTTAAGCGATTTATCCAGCCACAGAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCg                                                                                                                                <  1:1881716/150‑1 (MQ=255)
                      gtggCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCg                                                                                                                                <  2:1643946/150‑1 (MQ=255)
                      gtggCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCg                                                                                                                                <  1:1680581/150‑1 (MQ=255)
                      gtggCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCg                                                                                                                                >  1:1598329/1‑150 (MQ=255)
                         gCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCgtgt                                                                                                                             >  2:176619/1‑150 (MQ=255)
                              tAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAg                                                                                                                        <  1:996439/150‑1 (MQ=255)
                               agagCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGt                                                                                                                       >  1:292541/1‑150 (MQ=255)
                                gagCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTa                                                                                                                      <  1:1707527/150‑1 (MQ=255)
                                gagCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTa                                                                                                                      <  2:716076/150‑1 (MQ=255)
                                   cGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAg                                                                                                                   <  2:1873672/150‑1 (MQ=255)
                                   cGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAg                                                                                                                   >  1:196356/1‑150 (MQ=255)
                                     gCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAggg                                                                                                                 >  1:1362877/1‑150 (MQ=255)
                                      cTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGc                                                                                                                <  2:277495/150‑1 (MQ=255)
                                      cTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGc                                                                                                                >  2:683701/1‑150 (MQ=255)
                                          cAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGaa                                                                                                            >  2:726223/1‑150 (MQ=255)
                                            gTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACt                                                                                                          >  1:536178/1‑150 (MQ=255)
                                            gTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACt                                                                                                          <  1:424768/150‑1 (MQ=255)
                                              tttACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTgg                                                                                                        >  1:1766658/1‑150 (MQ=255)
                                                  cgcgAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGggcgg                                                                                                    >  1:1828975/1‑150 (MQ=255)
                                                  cgcgAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAAATGggcgg                                                                                                    >  1:333269/1‑150 (MQ=255)
                                                   gcgAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGggcggc                                                                                                   <  1:173152/150‑1 (MQ=255)
                                                    cgAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGgcggcg                                                                                                  >  2:1177437/1‑150 (MQ=255)
                                                    cgAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGgcggcg                                                                                                  >  1:1061174/1‑150 (MQ=255)
                                                    cgAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGgcggcg                                                                                                  <  1:1085064/150‑1 (MQ=255)
                                                     gAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGgcgc                                                                                                 <  1:1328885/150‑1 (MQ=255)
                                                       aTCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTa                                                                                               <  2:1661367/150‑1 (MQ=255)
                                                       aTCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTa                                                                                               <  1:683701/150‑1 (MQ=255)
                                                         ctgtctgACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTaaa                                                                                             >  1:1662829/1‑150 (MQ=255)
                                                          tgtctgACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTGGAGTAAAGGGCTGAACTGGGCGGCGCTAAAg                                                                                            >  2:361840/1‑150 (MQ=255)
                                                           gtctgACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGc                                                                                           <  1:1176643/150‑1 (MQ=255)
                                                             ctgACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGa                                                                                         >  1:1285743/1‑150 (MQ=255)
                                                              tgACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTTAACTGGGCGGCGCTAAAGCGAt                                                                                        >  2:1992793/1‑150 (MQ=255)
                                                               gACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATc                                                                                       >  2:999474/1‑150 (MQ=255)
                                                               gACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATc                                                                                       <  2:851019/150‑1 (MQ=255)
                                                                    ggCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCAAACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTg                                                                                  >  2:1522921/1‑150 (MQ=255)
                                                                        aGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCg                                                                              <  1:196631/150‑1 (MQ=255)
                                                                         ggTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCgg                                                                             >  2:1623244/1‑150 (MQ=255)
                                                                            tAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTc                                                                          >  2:224422/1‑150 (MQ=255)
                                                                            tAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGATGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTc                                                                          >  2:86823/1‑150 (MQ=255)
                                                                             aaTATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCa                                                                         >  2:321099/1‑150 (MQ=255)
                                                                               tatGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGc                                                                       <  1:1992793/150‑1 (MQ=255)
                                                                                    ctcACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTg                                                                  <  1:824923/150‑1 (MQ=255)
                                                                                      cACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTc                                                                               <  1:1546316/135‑1 (MQ=255)
                                                                                      cACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTc                                                                               >  2:1546316/1‑135 (MQ=255)
                                                                                      cACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTa                                                                >  2:1686446/1‑150 (MQ=255)
                                                                                        cccAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATc                                                              <  1:176619/150‑1 (MQ=255)
                                                                                         ccAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCg                                                             >  1:795443/1‑150 (MQ=255)
                                                                                              gcgATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGa                                                        >  1:1835509/1‑150 (MQ=255)
                                                                                                 aTTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACg                                                     <  1:1789648/150‑1 (MQ=255)
                                                                                                  tttttAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACgg                                                    <  2:196356/150‑1 (MQ=255)
                                                                                                   ttttAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGt                                                   <  1:658079/150‑1 (MQ=255)
                                                                                                     ttAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAg                                                 <  1:215779/150‑1 (MQ=255)
                                                                                                        aGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAgcgc                                              <  1:1623244/150‑1 (MQ=255)
                                                                                                         gCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAgcgcg                                             >  1:52070/1‑150 (MQ=255)
                                                                                                            aTTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTc                                          <  2:333269/150‑1 (MQ=255)
                                                                                                            aTTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTc                                          <  1:1210139/150‑1 (MQ=255)
                                                                                                               tATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGATTTGGGTTTATGTAAAGGGTTCGTGTGCTATGTTCAGGCTTTGTATCGCTTGAACGTTAGGTCGCTTaat                                       <  2:1598329/150‑2 (MQ=255)
                                                                                                               tATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTTAACGGTAGCGCGCTCAAc                                       <  2:1662829/150‑1 (MQ=255)
                                                                                                               tATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAAc                                       <  1:538462/150‑1 (MQ=255)
                                                                                                                aTCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACa                                      <  2:292250/150‑1 (MQ=255)
                                                                                                                     gCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATg                                 >  2:637723/1‑150 (MQ=255)
                                                                                                                     gCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATg                                 >  1:1515662/1‑150 (MQ=255)
                                                                                                                       cacacaGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGAc                               >  1:229210/1‑150 (MQ=255)
                                                                                                                       cacacaGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGAc                               >  2:1443294/1‑150 (MQ=255)
                                                                                                                              cccGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGc                        <  1:1177437/150‑1 (MQ=255)
                                                                                                                               ccGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGcc                       >  2:1500924/1‑150 (MQ=255)
                                                                                                                                    ccATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTg                  <  2:292541/150‑1 (MQ=255)
                                                                                                                                      aTACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGt                <  1:1659562/150‑1 (MQ=255)
                                                                                                                                        caacgcagATTATTAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTa              >  1:788715/5‑150 (MQ=255)
                                                                                                                                        caacgcagATTATTAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTa              >  2:1052378/5‑150 (MQ=255)
                                                                                                                                          acgcagATTATTAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTAAt            >  2:1599186/3‑150 (MQ=255)
                                                                                                                                           agcagATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTAAtt           >  2:139966/1‑150 (MQ=255)
                                                                                                                                            gcagATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTAAttt          >  1:1702572/1‑150 (MQ=255)
                                                                                                                                            gcagATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTAAttt          >  2:1151066/1‑150 (MQ=255)
                                                                                                                                                aTTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCAtt      <  2:1828975/150‑1 (MQ=255)
                                                                                                                                                  taataaTCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCAtttt    >  2:821262/1‑150 (MQ=255)
                                                                                                                                                    ataatCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCg  >  1:706364/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGAAACATAATTGTCTATTTTGGTGGCCATTAGAGCGGCTGACAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCG  >  NC_000913/1348905‑1349202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑