BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,579,563	G→T	6.3%	G7* (GGA→TGA)	yneP →	protein YneP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,579,563	0	G	T	6.3%	401.5 / 8.4	121	G7* (GGA→TGA)	yneP	protein YneP
Reads supporting (aligned to +/- strand): ref base G (63/49); new base T (3/6); total (66/55)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

ATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGGCGT  >  NC_000913/1579415‑1579712

aTTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAgg                                                                                                                                                      <  1:881035/150‑1 (MQ=255)

 tttAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGa                                                                                                                                                     >  2:1807116/1‑150 (MQ=255)

  ttAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGaa                                                                                                                                                    <  1:207743/150‑1 (MQ=255)

   tAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAAt                                                                                                                                                   <  1:1040726/150‑1 (MQ=255)

   tAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAAt                                                                                                                                                   <  2:8172/150‑1 (MQ=255)

   tAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAAt                                                                                                                                                   >  1:1613078/1‑150 (MQ=255)

   tAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAAt                                                                                                                                                   >  1:1772153/1‑150 (MQ=255)

     aCAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAAttt                                                                                                                                                 <  1:857405/150‑1 (MQ=255)

     aCAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGAATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACACGACAGGAAttt                                                                                                                                                 >  2:1700872/1‑150 (MQ=255)

      cAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTa                                                                                                                                                <  1:301099/150‑1 (MQ=255)

       aaTATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTAt                                                                                                                                               <  2:828968/150‑1 (MQ=255)

           tGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATTCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCg                                                                                                                                           <  2:618822/150‑1 (MQ=255)

           tGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCg                                                                                                                                           >  2:1315596/1‑150 (MQ=255)

            gAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCgg                                                                                                                                          >  1:1215411/1‑150 (MQ=255)

              aaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGt                                                                                                                                        >  2:623836/1‑150 (MQ=255)

               atatAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTg                                                                                                                                       <  1:1807116/150‑1 (MQ=255)

                tataAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGTgt                                                                                                                                      <  1:1406511/150‑2 (MQ=255)

                 ataAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGAAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGcc                                                                                                                                     >  1:112604/1‑150 (MQ=255)

                    atggAATATTTCATTTTCTGTTGTTTTGGTTATGCTATTGATTATAAAGGATATTGCTAATGCCCTGATGCATTCGACGTAAGCGAGTATTTTCTGAAGGGAAGATGATTATGACGAAACACGCGTCATGATTTTATGTCGGGTGCCTTg                                                                                                                                  <  2:1639241/148‑1 (MQ=255)

                    aaGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATAGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGTgtttcg                                                                                                                                  <  1:590048/150‑6 (MQ=255)

                      ggAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGtt                                                                                                                                >  2:692908/1‑150 (MQ=255)

                      ggAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGtt                                                                                                                                <  2:1750662/150‑1 (MQ=255)

                      ggAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGtt                                                                                                                                <  2:624431/150‑1 (MQ=255)

                          tatTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAgg                                                                                                                            <  2:1531875/150‑1 (MQ=255)

                           atTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGt                                                                                                                           >  1:168781/1‑150 (MQ=255)

                            tttCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGt                                                                                                                           >  2:1456840/1‑149 (MQ=255)

                            tttCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGt                                                                                                                           <  1:1456840/149‑1 (MQ=255)

                             ttCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGTTTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCa                                                                                                                         >  1:1923621/1‑150 (MQ=255)

                             ttCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCa                                                                                                                         >  2:866316/1‑150 (MQ=255)

                              tCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCAt                                                                                                                        >  2:1766047/1‑150 (MQ=255)

                              tCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCAGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCAt                                                                                                                        >  2:1641967/1‑150 (MQ=255)

                               cATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATa                                                                                                                       <  1:831729/150‑1 (MQ=255)

                                aTTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaa                                                                                                                      >  2:559198/1‑150 (MQ=255)

                                aTTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaa                                                                                                                      >  1:1760672/1‑150 (MQ=255)

                                  tttCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaaga                                                                                                                    >  2:286445/1‑150 (MQ=255)

                                        tggttatggttaTGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAaggagg                                                                                                              <  2:640807/150‑1 (MQ=255)

                                               ggttatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGaa                                                                                                       >  2:705536/1‑150 (MQ=255)

                                                 ttatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGTCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATg                                                                                                     <  2:1425019/150‑1 (MQ=255)

                                                  tatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGCGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATgg                                                                                                    <  1:660927/150‑1 (MQ=255)

                                                    tgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAg                                                                                                  <  1:1576907/150‑1 (MQ=255)

                                                     gCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCAACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGt                                                                                                 <  2:58492/150‑1 (MQ=255)

                                                      cTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGtt                                                                                                >  1:1257654/1‑150 (MQ=255)

                                                       tATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTa                                                                                               <  2:1625967/150‑1 (MQ=255)

                                                          tGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTGGCAAGGGAAGATGATGATGACGAAACACCCTAAATGAATTTATGTCGTGTGCCTTGTTAAGGTAATAAGAATGAGGCTAAGAATGTAGTTaaaa                                                                                            <  2:1109741/150‑1 (MQ=255)

                                                              aaTCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAagaga                                                                                        >  1:938288/1‑150 (MQ=255)

                                                                 cAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCg                                                                                     >  2:1311244/1‑150 (MQ=255)

                                                                 cAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCg                                                                                     >  1:1634898/1‑150 (MQ=255)

                                                                 cAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCg                                                                                     <  2:30282/150‑1 (MQ=255)

                                                                 cAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCg                                                                                     >  1:1593649/1‑150 (MQ=255)

                                                                    ggATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGtta                                                                                  >  2:500874/1‑150 (MQ=255)

                                                                     gttatTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGttat                                                                                 <  2:345059/148‑1 (MQ=255)

                                                                      atatTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGttatt                                                                                <  1:623836/150‑1 (MQ=255)

                                                                       tatTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGttatta                                                                               >  1:1978959/1‑150 (MQ=255)

                                                                         ttGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaat                                                                             <  2:1772153/150‑1 (MQ=255)

                                                                          tGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatt                                                                            >  2:733317/1‑150 (MQ=255)

                                                                           gCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatta                                                                           >  2:1109483/1‑150 (MQ=255)

                                                                           gCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatta                                                                           >  1:80609/1‑150 (MQ=255)

                                                                           gCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatta                                                                           <  1:692908/150‑1 (MQ=255)

                                                                            cTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAattat                                                                          >  2:1687458/1‑150 (MQ=255)

                                                                             tAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAattatt                                                                         >  1:1136626/1‑150 (MQ=255)

                                                                             tAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAattatt                                                                         >  1:1360601/1‑150 (MQ=255)

                                                                             tAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAattatt                                                                         >  1:1770900/1‑150 (MQ=255)

                                                                               aTGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCGAAGAATGGAGTTAAAAGAGAGCGTTATTTATTATtct                                                                       >  2:422150/1‑150 (MQ=255)

                                                                                tGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATtctc                                                                      <  2:112604/150‑1 (MQ=255)

                                                                                 gCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTcc                                                                     <  2:1346267/150‑1 (MQ=255)

                                                                                 gCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTcc                                                                     <  1:615188/150‑1 (MQ=255)

                                                                                  cccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCa                                                                    <  2:1387388/150‑1 (MQ=255)

                                                                                  cccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCa                                                                    >  1:1387388/1‑150 (MQ=255)

                                                                                   ccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCAt                                                                   <  1:500874/150‑1 (MQ=255)

                                                                                      gATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACGCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTg                                                                <  2:650379/150‑1 (MQ=255)

                                                                                      gATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTg                                                                >  1:1535965/1‑150 (MQ=255)

                                                                                           aTACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTg                                                           >  2:926717/1‑150 (MQ=255)

                                                                                             aCCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCa                                                         <  1:1798319/150‑1 (MQ=255)

                                                                                              ccACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTTAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCaa                                                        >  2:1503383/1‑150 (MQ=255)

                                                                                              ccACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCaa                                                        <  1:31440/150‑1 (MQ=255)

                                                                                                  gTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATc                                                    <  1:1441221/150‑1 (MQ=255)

                                                                                                   tAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATcc                                                   >  1:502618/1‑150 (MQ=255)

                                                                                                   tAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATcc                                                   <  2:807795/150‑1 (MQ=255)

                                                                                                       ccAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAACAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATaa                                               >  1:1768406/1‑150 (MQ=255)

                                                                                                           gATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAAtgtg                                           <  1:50178/150‑1 (MQ=255)

                                                                                                            aTTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTgg                                          >  1:1932984/1‑150 (MQ=255)

                                                                                                                 cGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAg                                     >  1:1510102/1‑150 (MQ=255)

                                                                                                                  gCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGc                                    >  2:1454458/1‑150 (MQ=255)

                                                                                                                    aaGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCgg                                  <  1:1315596/150‑1 (MQ=255)

                                                                                                                        gAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGGGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTAct                              >  2:421897/1‑150 (MQ=255)

                                                                                                                        gAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTAct                              >  1:1385895/1‑150 (MQ=255)

                                                                                                                         aaGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAAAATCCATAATGTGGTAAAGCGGTActc                             >  2:379533/1‑150 (MQ=255)

                                                                                                                          aGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTActct                            >  1:1823030/1‑150 (MQ=255)

                                                                                                                          aGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTActct                            >  2:287927/1‑150 (MQ=255)

                                                                                                                                  atgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAg                    >  2:1909690/1‑150 (MQ=255)

                                                                                                                                   tgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGc                   >  2:1285722/1‑150 (MQ=255)

                                                                                                                                   tgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGc                   <  2:1760672/150‑1 (MQ=255)

                                                                                                                                    gaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCt                  <  2:1978959/150‑1 (MQ=255)

                                                                                                                                      cGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTaa                >  2:1793760/1‑150 (MQ=255)

                                                                                                                                        aggcacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAAct              >  1:53212/4‑150 (MQ=255)

                                                                                                                                        aaaCACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATTGAGTTAAAAGAGAGCGTTATTAATTAATCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAAct              <  2:1013711/150‑1 (MQ=255)

                                                                                                                                             cccGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTAcc         >  1:293052/1‑150 (MQ=255)

                                                                                                                                                 aCAGGAATTTATGTCTTGTGCCTTGTTAAGGTCATAAGAAGTAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATgg     <  1:1700872/150‑1 (MQ=255)

                                                                                                                                                 aCAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATgg     <  1:286445/150‑1 (MQ=255)

                                                                                                                                                  cAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGGc    >  2:506674/1‑150 (MQ=255)

ATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGGCGT  >  NC_000913/1579415‑1579712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`