Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,901,694 A→T 5.4% F30Y (TTT→TAT) ‡ yoaL ← protein YoaL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,901,6940AT 5.4% 237.3 / 3.0 74F30Y (TTT→TAT) ‡yoaLprotein YoaL
Reads supporting (aligned to +/- strand):  ref base A (33/37);  new base T (2/2);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGA  >  NC_000913/1901549‑1901843
                                                                                                                                                 |                                                                                                                                                     
gCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAgg                                                                                                                                                   >  1:1566718/1‑150 (MQ=255)
  cccGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGcc                                                                                                                                                 >  2:959039/1‑150 (MQ=255)
   ccGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTaaaa                                                                                                                                                     >  2:1216861/1‑145 (MQ=255)
   ccGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTaaaa                                                                                                                                                     <  1:1216861/145‑1 (MQ=255)
      cccAAATTGAGGTGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGACAGAAATAATGCAGGAGTAACTAAAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGAt                                                                                                                                             >  1:1762816/1‑150 (MQ=255)
      cccAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCCTTTTAACGCCTgcc                                                                                                                                             <  2:1198130/150‑3 (MQ=255)
        cAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGc                                                                                                                                           >  1:572670/1‑150 (MQ=255)
        cAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGATTAAGGAATAGCTATTCGACGCCAGAAATAATGAAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGATAAAAGGCCTGATGc                                                                                                                                           >  1:691987/1‑150 (MQ=255)
         aaaTTGAGGGGTCCATTTAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCt                                                                                                                                          >  1:1509240/1‑150 (MQ=255)
           aTTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGa                                                                                                                                        <  1:1248449/150‑1 (MQ=255)
           aTTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCCTTTTAACGCCTgcctttct                                                                                                                                        <  1:491993/150‑8 (MQ=255)
                     ccATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCg                                                                                                                              <  1:949866/150‑1 (MQ=255)
                       aTTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGt                                                                                                                            >  1:345420/1‑150 (MQ=255)
                       aTTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGt                                                                                                                            >  2:1164587/1‑150 (MQ=255)
                       aTTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGt                                                                                                                            <  2:1356133/150‑1 (MQ=255)
                       aTTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGt                                                                                                                            >  2:1529381/1‑150 (MQ=255)
                          aaGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATg                                                                                                                                    <  1:546096/139‑1 (MQ=255)
                          aaGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATg                                                                                                                                    >  2:546096/1‑139 (MQ=255)
                          aaGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGAc                                                                                                                         <  2:1566718/150‑1 (MQ=255)
                             aaTTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGAt                                                                                                                      <  1:1371174/150‑1 (MQ=255)
                               ttCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATcc                                                                                                                    <  1:1529381/150‑1 (MQ=255)
                               ttCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATcc                                                                                                                    <  1:1164587/150‑1 (MQ=255)
                                tCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCa                                                                                                                   >  2:1686290/1‑150 (MQ=255)
                                tCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCa                                                                                                                   <  1:1340526/150‑1 (MQ=255)
                                  cATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATa                                                                                                                 <  1:949450/150‑1 (MQ=255)
                                   aTGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATAc                                                                                                                <  1:1022730/150‑1 (MQ=255)
                                      aCAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGc                                                                                                             >  1:1579432/1‑150 (MQ=255)
                                      aCAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGc                                                                                                             >  2:35842/1‑150 (MQ=255)
                                       cAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCg                                                                                                            >  2:1169707/1‑150 (MQ=255)
                                         aGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCggg                                                                                                          >  1:910289/1‑150 (MQ=255)
                                                gCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGc                                                                                                   <  1:215007/150‑1 (MQ=255)
                                                 cTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGcc                                                                                                  >  1:1029934/1‑150 (MQ=255)
                                                  ttAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGccc                                                                                                 <  2:1509240/150‑1 (MQ=255)
                                                  ttAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGccc                                                                                                 <  1:1169707/150‑1 (MQ=255)
                                                     aGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCtat                                                                                              >  2:1529250/1‑150 (MQ=255)
                                                      ggAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCtata                                                                                             >  1:1381327/1‑150 (MQ=255)
                                                          tAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTcc                                                                                         >  2:1895900/1‑150 (MQ=255)
                                                          tAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTcc                                                                                         <  1:767964/150‑1 (MQ=255)
                                                           aGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCa                                                                                        <  1:712564/150‑1 (MQ=255)
                                                               aTTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGgt                                                                                    >  2:142497/1‑150 (MQ=255)
                                                                  cGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGgttgt                                                                                 >  1:663376/1‑150 (MQ=255)
                                                                  cGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGgttgt                                                                                 >  1:823867/1‑150 (MQ=255)
                                                                   gACGCCAGTAATAATGCAGGAGTAACGACAAAATGCAAACGAAAGGTGCGTGATATAGTGCCAGAAATGCAGGGGTTTAAATGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTTCGGGCTACTGCCCTATACTCCATGgttgtt                                                                                <  2:691987/150‑1 (MQ=255)
                                                                   gACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGgttgtt                                                                                <  2:254655/150‑1 (MQ=255)
                                                                    aagCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAATGTTCGGCATAGAGTGCCAGAAAGGCAGGCTTTAAAAGGCCTGTTGCTGAAATGACGTCGTTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTa                                                                               <  2:1735679/148‑1 (MQ=255)
                                                                       ccAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAAc                                                                            >  1:43990/1‑150 (MQ=255)
                                                                       ccAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAAc                                                                            >  1:615730/1‑150 (MQ=255)
                                                                       ccAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAAc                                                                            >  1:622047/1‑150 (MQ=255)
                                                                        cAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACg                                                                           >  2:1427848/1‑150 (MQ=255)
                                                                        cAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACg                                                                           <  2:43990/150‑1 (MQ=255)
                                                                            aataatGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAg                                                                       <  1:1529250/150‑1 (MQ=255)
                                                                               aatGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTa                                                                    <  1:142497/150‑1 (MQ=255)
                                                                                atGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTaa                                                                   <  2:663376/150‑1 (MQ=255)
                                                                                  ccAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGGCTGATTCTGAAATGACGTCTGTGACGATCCATACTGCGGGCTACTTCCCTATACTCCATGGTTTTTATACGTGAGTTAAAc                                                                 <  2:1694799/149‑1 (MQ=255)
                                                                                       cGTAACGACAAAATGTAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATc                                                            >  1:1750037/1‑150 (MQ=255)
                                                                                        gTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCa                                                           >  1:1972024/1‑150 (MQ=255)
                                                                                         tAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCag                                                          <  2:144688/150‑1 (MQ=255)
                                                                                         tAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCag                                                          <  1:959039/150‑1 (MQ=255)
                                                                                                        aaCGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGAttt                                           >  2:1135825/1‑150 (MQ=255)
                                                                                                        aaCGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGAttt                                           >  1:658388/1‑150 (MQ=255)
                                                                                                          cGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGGCGTCTCTGATATgt                                         <  2:1190356/150‑2 (MQ=255)
                                                                                                           gAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGc                                        <  1:113824/150‑1 (MQ=255)
                                                                                                           gAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGc                                        <  1:1920101/150‑1 (MQ=255)
                                                                                                                  gCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGAtt                                 <  2:910289/150‑1 (MQ=255)
                                                                                                                    ggCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTa                               <  2:572670/150‑1 (MQ=255)
                                                                                                                            gTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTAccttcctt                       <  1:1738544/150‑1 (MQ=255)
                                                                                                                                cAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCa                   <  1:530262/150‑1 (MQ=255)
                                                                                                                                cAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCa                   <  2:1602273/150‑1 (MQ=255)
                                                                                                                                    gcatcaggcCTTTTAACGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGCCGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACg               >  1:1307982/5‑150 (MQ=255)
                                                                                                                                    gcatcaggcCTTTTAACGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACg               >  1:536591/5‑150 (MQ=255)
                                                                                                                                     aggcaggcGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGa              <  1:1427848/150‑1 (MQ=255)
                                                                                                                                      ggcaggcGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGaa             >  1:1072648/1‑150 (MQ=255)
                                                                                                                                       gcaggcGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCATAGATTTACCTTCCTTTGCAAACGAAt            >  1:645349/1‑150 (MQ=255)
                                                                                                                                          ggcTTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATTGTTGTTAAACGGGATTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAAtgtg         <  2:1762816/150‑1 (MQ=255)
                                                                                                                                          ggcGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAAtgtg         >  1:680153/1‑150 (MQ=255)
                                                                                                                                           gcGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGa        <  2:1381327/150‑1 (MQ=255)
                                                                                                                                                 aaaGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGa  >  2:277948/1‑150 (MQ=255)
                                                                                                                                                 aaaGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGa  >  1:321097/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATGCAGGCGTAACGACAAAATGCAAACGAAAGGTGCGGCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGA  >  NC_000913/1901549‑1901843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑