Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,928,799	A→C	7.3%	L14R (CTT→CGT)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,928,799	0	A	C	7.3%	243.7 / 11.1	82	L14R (CTT→CGT)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand):  ref base A (42/34);  new base C (4/2);  total (46/36)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

CCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGAT  >  NC_000913/1928650‑1928948                                                                                                                                                      |                                                                                                                                                      ccTTTAAGATTCGATCCTAAAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTATTATTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGaa                                                                                                                                                       >  2:1949840/1‑150 (MQ=255)  cTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAg                                                                                                                                                      >  2:59092/1‑150 (MQ=255)      aaGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTc                                                                                                                                                  >  1:1957480/1‑150 (MQ=255)       aGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCa                                                                                                                                                 <  2:1087432/150‑1 (MQ=255)          tGCGATCCTGCAAGGCTTGTTTTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATgg                                                                                                                                              <  2:1537482/150‑1 (MQ=255)              aTCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTg                                                                                                                                          <  1:476914/150‑1 (MQ=255)              aTCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTg                                                                                                                                          <  1:309675/150‑1 (MQ=255)                 cTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTgggg                                                                                                                                       <  1:227023/150‑1 (MQ=255)                  tGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCgatacgc                                                                                                                                      <  1:741128/150‑7 (MQ=255)                  tGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGa                                                                                                                                      >  2:1747370/1‑150 (MQ=255)                  tGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGa                                                                                                                                      >  1:922944/1‑150 (MQ=255)                        gCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgg                                                                                                                                >  2:1702576/1‑150 (MQ=255)                            gttgtGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgggcgg                                                                                                                            >  1:937229/1‑150 (MQ=255)                             ttgtGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGggcggc                                                                                                                           >  2:784985/1‑150 (MQ=255)                             ttgtGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGggcggc                                                                                                                           <  2:1957480/150‑1 (MQ=255)                               gtgAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCtt                                                                                                                         >  2:1787167/1‑150 (MQ=255)                                 gAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCtttt                                                                                                                       <  1:59092/150‑1 (MQ=255)                                     ccATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTa                                                                                                                   >  2:814005/1‑150 (MQ=255)                                      cATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATTGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAg                                                                                                                  >  2:325371/1‑150 (MQ=255)                                       aTCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGc                                                                                                                 <  2:878567/150‑1 (MQ=255)                                          aCCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGTCGGCTTTTGGTATCATg                                                                                                              <  2:344479/150‑1 (MQ=255)                                          aCCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGt                                                                                                                    >  2:579559/1‑144 (MQ=255)                                          aCCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGt                                                                                                                    <  1:579559/144‑1 (MQ=255)                                           cccGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGt                                                                                                             <  1:1735333/150‑1 (MQ=255)                                             cGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTa                                                                                                           <  2:1529894/150‑1 (MQ=255)                                                   ccGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttc                                                                                                     >  2:420638/1‑150 (MQ=255)                                                    cGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttct                                                                                                    >  1:1460283/1‑150 (MQ=255)                                                     gTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctt                                                                                                   >  1:1645429/1‑150 (MQ=255)                                                         cTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTg                                                                                               >  2:1468115/1‑150 (MQ=255)                                                          tATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTgg                                                                                              <  1:1230907/150‑1 (MQ=255)                                                            ttttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGtt                                                                                            >  2:1774511/1‑150 (MQ=255)                                                              ttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTgt                                                                                          <  2:1237264/150‑2 (MQ=255)                                                              ttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGa                                                                                          >  2:943545/1‑150 (MQ=255)                                                                gttgttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCTCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGaaa                                                                                        <  1:1676514/149‑1 (MQ=255)                                                                   gttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACAt                                                                                     >  1:1240055/1‑150 (MQ=255)                                                                     tgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGTTTGAAACATCt                                                                                   <  2:87610/150‑1 (MQ=255)                                                                     tgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCt                                                                                   <  1:1787167/150‑1 (MQ=255)                                                                     tgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCt                                                                                   >  2:406659/1‑150 (MQ=255)                                                                     tgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCt                                                                                   >  1:387189/1‑150 (MQ=255)                                                                      gCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCtt                                                                                  <  2:1645429/150‑1 (MQ=255)                                                                         ggTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTtata                                                                               <  1:784985/150‑1 (MQ=255)                                                                         ggTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTtata                                                                               >  1:791847/1‑150 (MQ=255)                                                                         ggTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTtata                                                                               >  1:1342012/1‑150 (MQ=255)                                                                             gTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAggg                                                                           >  1:1272406/1‑150 (MQ=255)                                                                             gTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCATATAAggg                                                                           >  1:1227670/1‑150 (MQ=255)                                                                                cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTgg                                                                        >  1:1669891/1‑150 (MQ=255)                                                                                 aGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGc                                                                       <  2:1272406/150‑1 (MQ=255)                                                                                     cTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATAGAAGCGCGTATCGCCATGAAGCGTCATGGCCTGGGGAATGCGGGCGGCTTTTGGTAGCATCTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                                   >  2:312324/1‑150 (MQ=255)                                                                                      ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAAc                                                                  <  1:1665292/150‑1 (MQ=255)                                                                                       tCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACt                                                                 >  1:1868644/1‑150 (MQ=255)                                                                                        cTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGAAGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTc                                                                >  1:673213/1‑150 (MQ=255)                                                                                              gagaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCggg                                                          >  2:667470/1‑150 (MQ=255)                                                                                               agaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCgggg                                                         <  2:937229/150‑1 (MQ=255)                                                                                               agaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCgggg                                                         >  2:1112924/1‑150 (MQ=255)                                                                                                gaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGa                                                        >  1:1595650/1‑150 (MQ=255)                                                                                                      gTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGGGGCAAAACTCACCGGGGATGCAAg                                                  <  2:1532354/150‑1 (MQ=255)                                                                                                       tATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGc                                                 >  1:1104285/1‑150 (MQ=255)                                                                                                       tATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGc                                                 <  1:1393985/150‑1 (MQ=255)                                                                                                        aTCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCg                                                <  1:1949840/150‑1 (MQ=255)                                                                                                         tCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGa                                               <  1:1277955/150‑1 (MQ=255)                                                                                                           gTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGaaa                                             >  1:22385/1‑150 (MQ=255)                                                                                                            tCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAAc                                            <  2:1868644/150‑1 (MQ=255)                                                                                                                 cAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAgggg                                       <  2:387189/150‑1 (MQ=255)                                                                                                                   gCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAg                                     >  2:17338/1‑150 (MQ=255)                                                                                                                     cAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTc                                   >  1:1246077/1‑150 (MQ=255)                                                                                                                     cAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTc                                   >  1:1089161/1‑150 (MQ=255)                                                                                                                     cAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGGTATTTCTGGGTGAAGAATCTTATTAGGGTCGCAAAAGTCACGGGGGATGGGAGGGAAACAGGGGTGTc                                   >  2:867515/1‑150 (MQ=255)                                                                                                                      agtagtAATTATCGAAGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCa                                  <  2:673213/150‑1 (MQ=255)                                                                                                                            aaTTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCtt                            >  1:362438/1‑150 (MQ=255)                                                                                                                             aTTCTCGATGCGCTTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTa                           <  1:867515/150‑1 (MQ=255)                                                                                                                             aTTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTa                           <  1:814005/150‑1 (MQ=255)                                                                                                                               tATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAga                         <  1:469867/150‑1 (MQ=255)                                                                                                                                aTCGATGCGCGTATCGCAATGAAGCGACATGGCGTGGGGAATGCGGGCGGCATTTGGTAGCATGTTATTGTTCTATCAGGTTGAAACATCTTATAAGGGTGGCAAAACTCAACGCAGATGCAAGCGAAACAGGCGAGTCATTGATTacaa                        >  2:244898/1‑147 (MQ=255)                                                                                                                                    aTGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAGTGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGatgatg                    <  2:922944/150‑1 (MQ=255)                                                                                                                                    aTGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGatgatg                    <  1:1747370/150‑1 (MQ=255)                                                                                                                                           tATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTa             >  2:333196/1‑150 (MQ=255)                                                                                                                                           ccacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTa             >  2:1744459/4‑150 (MQ=255)                                                                                                                                           ccacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTa             >  1:442986/4‑150 (MQ=255)                                                                                                                                             tCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAAt           >  1:1970035/1‑150 (MQ=255)                                                                                                                                             acGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAAt           >  1:604798/2‑150 (MQ=255)                                                                                                                                                ccATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGgc        >  1:474137/1‑150 (MQ=255)                                                                                                                                                  aTGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGgcgc      >  2:1843937/1‑150 (MQ=255)                                                                                                                                                   tGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGcgcg     <  1:943545/150‑1 (MQ=255)                                                                                                                                                      aGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGat  >  1:1710567/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                      CCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGAT  >  NC_000913/1928650‑1928948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑