Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,130,930	A→G	11.1%	F381L (TTC→CTC)	wcaC ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,130,930	0	A	G	11.1%	166.8 / 11.0	63	F381L (TTC→CTC)	wcaC	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (24/32);  new base G (4/3);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

ATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATGGGTGGCAATCACCGGCA  >  NC_000913/2130781‑2131079                                                                                                                                                      |                                                                                                                                                      aTGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGAAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGaa                                                                                                                                                       <  1:799029/150‑1 (MQ=255)  tGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAAc                                                                                                                                                      <  1:1155503/150‑1 (MQ=255)  tGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAAc                                                                                                                                                      <  1:1410838/150‑1 (MQ=255)  tGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAAc                                                                                                                                                      >  1:205064/1‑150 (MQ=255)        aaCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGTATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTcagcc                                                                                                                                                >  1:871998/1‑150 (MQ=255)          cTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTcagccag                                                                                                                                              >  2:155674/1‑150 (MQ=255)          cTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTcagccag                                                                                                                                              >  1:110720/1‑150 (MQ=255)              cGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTg                                                                                                                                          >  1:752988/1‑150 (MQ=255)              cGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGCTGGCTGAGTTCAGCCAACgca                                                                                                                                          <  1:1623422/150‑3 (MQ=255)               gCCAAGCTGGGCGATTTTGACGTTAACCATCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAGTTCAGCCAACgcag                                                                                                                                         <  2:617370/150‑4 (MQ=255)                ccAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGt                                                                                                                                        >  1:1829401/1‑150 (MQ=255)                   aGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGGCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTAcc                                                                                                                                     <  1:264598/150‑1 (MQ=255)                     cTGGGCGATTTTGACGTTAATCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCACCCAGCGTGGTACCaa                                                                                                                                   <  1:903547/150‑1 (MQ=255)                      tGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCaaa                                                                                                                                  <  1:1053356/150‑1 (MQ=255)                         gCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAatat                                                                                                                               >  1:1880289/1‑150 (MQ=255)                           gATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCg                                                                                                                             <  2:777125/150‑1 (MQ=255)                             ttttGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGcc                                                                                                                           >  1:459966/1‑150 (MQ=255)                                tGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAGTTCAGCCAACg                                                                                                                                            <  2:539629/130‑1 (MQ=255)                                tGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAGTTCAGCCAACg                                                                                                                                            >  1:539629/1‑130 (MQ=255)                                 gACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTg                                                                                                                         <  2:250082/148‑1 (MQ=255)                                 gACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTg                                                                                                                         >  1:250082/1‑148 (MQ=255)                                   cGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGcgcg                                                                                                                     >  1:510157/1‑150 (MQ=255)                                      tAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGAtt                                                                                                                  <  2:727811/150‑1 (MQ=255)                                           agcagATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCgg                                                                                                             <  1:1672062/150‑1 (MQ=255)                                              agATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGttt                                                                                                          <  2:475424/150‑1 (MQ=255)                                               gATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATGGCCTGCTGGATTTCCGGTTTg                                                                                                         <  2:752988/150‑1 (MQ=255)                                               gATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTg                                                                                                         >  2:958427/1‑150 (MQ=255)                                               gATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTg                                                                                                         >  2:1207743/1‑150 (MQ=255)                                               gATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTg                                                                                                         >  2:1882816/1‑150 (MQ=255)                                                 tAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCt                                                                                                       >  2:530259/1‑150 (MQ=255)                                                          ggCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCAc                                                                                              <  2:245020/150‑1 (MQ=255)                                                             agcaACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAg                                                                                           <  1:1862077/150‑1 (MQ=255)                                                                 aCAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTgc                                                                                       >  2:1029167/1‑150 (MQ=255)                                                                 aCAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTgc                                                                                       <  2:509846/150‑1 (MQ=255)                                                                   aGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTgcag                                                                                     <  2:205064/150‑1 (MQ=255)                                                                    ggTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTgcagc                                                                                    <  2:510157/150‑1 (MQ=255)                                                                     ttAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTgcagca                                                                                   <  1:480480/149‑1 (MQ=255)                                                                       aGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCAcc                                                                                 <  1:155674/150‑1 (MQ=255)                                                                        gCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACct                                                                                <  1:543230/150‑1 (MQ=255)                                                                        gCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACct                                                                                <  2:1829401/150‑1 (MQ=255)                                                                          tACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACctct                                                                              >  2:885204/1‑150 (MQ=255)                                                                          tACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACctct                                                                              >  1:1748248/1‑150 (MQ=255)                                                                          tACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACctct                                                                              <  2:1748248/150‑1 (MQ=255)                                                                          tACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACctct                                                                              >  2:191293/1‑150 (MQ=255)                                                                              gATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTcttctt                                                                          >  2:833601/1‑150 (MQ=255)                                                                               aTTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCttcttc                                                                         <  1:1974824/150‑1 (MQ=255)                                                                                 tCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGc                                                                       <  2:505484/150‑1 (MQ=255)                                                                                    gATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGa                                                                    >  2:1034357/1‑150 (MQ=255)                                                                                          aGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGttt                                                              >  2:735294/1‑150 (MQ=255)                                                                                                  tACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTAccgccg                                                      <  1:885204/150‑1 (MQ=255)                                                                                                   aCTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCgg                                                     <  2:316161/150‑1 (MQ=255)                                                                                                     tcctccAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGAt                                                   >  2:685209/1‑150 (MQ=255)                                                                                                                     gtCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTccc                                   <  2:1821566/150‑1 (MQ=255)                                                                                                                       ccACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCcgc                                 >  2:1574739/1‑150 (MQ=255)                                                                                                                       ccACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCcgc                                 >  1:958144/1‑150 (MQ=255)                                                                                                                                  ggcgcggcTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCt                      <  1:1207743/150‑1 (MQ=255)                                                                                                                                  ggcgcggcTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCt                      <  1:1882816/150‑1 (MQ=255)                                                                                                                                     gcggcTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATg                   <  1:1574739/150‑1 (MQ=255)                                                                                                                                        gcTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATGGGt                <  1:991143/150‑1 (MQ=255)                                                                                                                                         ccacgctGGCTGAGTTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATGGGTg               >  1:1199766/7‑150 (MQ=255)                                                                                                                                         ccacgctGGCTGAGTTCAGCCAACGTGGTACCAAATATCGCCTGCGCGATTTCCAGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATGGGTg               >  2:997291/7‑150 (MQ=255)                                                                                                                                            cGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATGGGTGGCa            <  1:191293/150‑1 (MQ=255)                                                                                                                                             gctGGCTGAGTTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATGGGTGGCaa           >  1:947344/3‑150 (MQ=255)                                                                                                                                                   tGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGATGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATGGGTGGCAATCACCg     <  1:1029167/150‑1 (MQ=255)                                                                                                                                                      aCTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATg                   >  2:109360/1‑133 (MQ=255)                                                                                                                                                      aCTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATg                   <  1:109360/133‑1 (MQ=255)                                                                                                                                                      aCTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATGGGTGGCAATCACCGGCa  >  1:1857353/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                      ATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTTGCTTAACTGCACCAGTTGCAGCACCTCTTCTTCGCTGACGGTTTTACCGCCGGATTTTTGCAACACTTCCCGCGCCGCATCGCTATGGGTGGCAATCACCGGCA  >  NC_000913/2130781‑2131079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑