| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,281,193 | C→T | 6.9% | R188C (CGT→TGT) | radD → | putative DNA repair helicase RadD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,281,193 | 0 | C | T | 6.9% | 177.5 / 2.6 | 58 | R188C (CGT→TGT) | radD | putative DNA repair helicase RadD |
| Reads supporting (aligned to +/- strand): ref base C (24/30); new base T (2/2); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01 | |||||||||||
TGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAA > NC_000913/2281050‑2281336 | tGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTtata < 1:1764982/150‑1 (MQ=255) aaTCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATg < 2:1040412/150‑1 (MQ=255) tCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGAt < 1:38860/150‑1 (MQ=255) ccccACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGAtt > 2:1956340/1‑150 (MQ=255) cccACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTa < 1:950354/150‑1 (MQ=255) cTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAAAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAaca > 2:1105582/1‑150 (MQ=255) cGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGc < 1:698264/150‑1 (MQ=255) tgctgGGGCTGACTGACACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAAAACgcataaa > 2:88093/1‑144 (MQ=255) gctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCt > 1:1614568/1‑150 (MQ=255) gctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCt < 1:1067365/150‑1 (MQ=255) ctgGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTg > 1:1812883/1‑150 (MQ=255) ggCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCC‑TTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGAcgcc < 2:681339/150‑1 (MQ=255) cggACTGCCACGCCGTTTCGATTGGGCAAAGGCGGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGAcgccg < 2:1294725/148‑1 (MQ=255) cTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGAcgccg < 2:269883/150‑1 (MQ=255) tGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGAcgccgc < 2:196682/150‑1 (MQ=255) gACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACgccgcc > 2:1557599/1‑150 (MQ=255) gACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACgccgcc < 2:1091138/150‑1 (MQ=255) gACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACgccgcc < 2:1075018/150‑1 (MQ=255) gCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGaa > 1:329475/1‑150 (MQ=255) ccACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAAc < 1:1067285/150‑1 (MQ=255) cACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACg < 2:1614568/150‑1 (MQ=255) cTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTgg > 1:554877/1‑150 (MQ=255) cTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTgg < 2:1812883/150‑1 (MQ=255) cGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGAcg > 2:127756/1‑129 (MQ=255) cGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGAcg < 1:127756/129‑1 (MQ=255) aTTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGcc < 1:481946/150‑1 (MQ=255) aTTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGcc < 1:1956340/150‑1 (MQ=255) ggCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCagta > 2:327426/1‑150 (MQ=255) ggCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCagta > 2:1114910/1‑150 (MQ=255) aaGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGc < 2:297794/150‑1 (MQ=255) tGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATAc < 1:1855187/150‑1 (MQ=255) ggATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACg > 1:1944830/1‑150 (MQ=255) cTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGAttt > 1:869725/1‑150 (MQ=255) aCCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATTTTTTTAAAAACGGCTATCTTACGAGTCCATAACGACTGTATATTCTAGTAGTTCAATACGATTTCa < 2:509914/150‑1 (MQ=255) ttCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGTGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGc > 1:718391/1‑132 (MQ=255) ttCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGTGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGc < 2:711320/132‑1 (MQ=255) ttCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGTGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGc > 1:711320/1‑132 (MQ=255) ttCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGTGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGc < 2:718391/132‑1 (MQ=255) aTCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAgg > 2:1227571/1‑150 (MQ=255) cACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGca > 1:1023248/1‑150 (MQ=255) cACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGca > 1:82783/1‑150 (MQ=255) ggCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACag < 2:486745/150‑1 (MQ=255) cggcgATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCt > 1:1409746/1‑150 (MQ=255) aTGAAAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCa < 1:1105582/150‑1 (MQ=255) tGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAg > 1:1865082/1‑150 (MQ=255) agaAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTg > 1:602166/1‑143 (MQ=255) agaAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTg < 2:602166/143‑1 (MQ=255) agaAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCg > 1:1095528/1‑150 (MQ=255) aGCCCCTTTCCGTTCATTCATTTATTCTCTGCCGGTTCGTTATATTATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGAGaata < 1:88093/150‑3 (MQ=255) cccTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCg < 2:795486/150‑1 (MQ=255) ttttCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGAtc < 2:1643304/150‑1 (MQ=255) aCTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTg > 1:1847856/1‑150 (MQ=255) aGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaac > 1:233781/1‑150 (MQ=255) gCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGTCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaaca < 2:215832/150‑1 (MQ=255) gCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaaca > 1:440281/1‑150 (MQ=255) gCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCACTAGTGCAATACGATTTCAGCCGCTTGCATGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaaca > 2:402419/1‑150 (MQ=255) gCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaaca < 2:1409746/150‑1 (MQ=255) ccGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAaacaacaa < 1:1557599/150‑1 (MQ=255) | TGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTTTCCGTGACTGCATTTATGAGCTGCCGCTGCGTTATATGATTAAACACGGCTATCTGACGCCGCCAGAACGACTGGATATGCCAGTAGTGCAATACGATTTCAGCCGCTTGCAGGCACAGAGTAACGGGCTGTTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAA > NC_000913/2281050‑2281336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |