Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,410,902 T→G 5.8% T151P (ACG→CCG)  yfbS ← putative transporter YfbS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,410,9020TG 5.8% 256.9 / 5.5 83T151P (ACG→CCG) yfbSputative transporter YfbS
Reads supporting (aligned to +/- strand):  ref base T (39/39);  new base G (3/2);  total (42/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGA  >  NC_000913/2410755‑2411048
                                                                                                                                                   |                                                                                                                                                  
gCATGAAACGCATCACTAACATATACAAAATAACCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTAACAGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGtca                                                                                                                                                  >  1:1669591/1‑150 (MQ=255)
  aTGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGtcatc                                                                                                                                                >  1:126542/1‑150 (MQ=255)
    gAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTcatcat                                                                                                                                              <  2:404646/150‑1 (MQ=255)
    gAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTcatcat                                                                                                                                              >  2:924254/1‑150 (MQ=255)
         gCATCACTAACATTTACAAAATACCCAGCACCAGCACGACCAGGCCAAGAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGc                                                                                                                                         <  1:1940880/150‑1 (MQ=255)
          cATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCt                                                                                                                                        <  2:306388/150‑1 (MQ=255)
               cTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCa                                                                                                                                   >  2:1447528/1‑150 (MQ=255)
                 aaCATATACAAAATACCCAGCACCAGCACGCCCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGc                                                                                                                                 >  1:828638/1‑150 (MQ=255)
                   cATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGccc                                                                                                                               <  1:1903749/150‑1 (MQ=255)
                     tataCAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCgg                                                                                                                             >  2:484586/1‑150 (MQ=255)
                       taCAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCa                                                                                                                           >  2:1248557/1‑150 (MQ=255)
                       taCAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCa                                                                                                                           >  2:171384/1‑150 (MQ=255)
                         cAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCaaa                                                                                                                         >  1:1366295/1‑150 (MQ=255)
                         cAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCaaa                                                                                                                         >  2:177698/1‑150 (MQ=255)
                           aaaTACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAAc                                                                                                                       <  2:1108184/150‑1 (MQ=255)
                            aaTACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACt                                                                                                                      >  2:334818/1‑150 (MQ=255)
                              tACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTa                                                                                                                    <  2:520500/150‑1 (MQ=255)
                              tACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTa                                                                                                                    <  2:21955/150‑1 (MQ=255)
                                cccAGGACTAGCACGACCATGCCATTAGGTGTTACGATAAAGAAAGTGAAGACGTGATAGCCTTCACGCAGGAATTCACTGTTGTCTACCATGTTCGGCGGCGTCGCCACGAGCGTCATCATGCCGCTAATCAGCCCGGGAAAACTTAAc                                                                                                                  <  2:1669591/150‑1 (MQ=255)
                                  cagcaccagcacGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACgg                                                                                                                >  2:435253/1‑150 (MQ=255)
                                    gcaccagcacGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGca                                                                                                              <  1:580313/150‑1 (MQ=255)
                                       ccagcacGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCCGCAAAACTTAACGGcatca                                                                                                           >  1:1060180/1‑150 (MQ=255)
                                         agcacGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCatcatc                                                                                                         >  1:121163/1‑150 (MQ=255)
                                             cGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGAc                                                                                                     >  1:1733500/1‑150 (MQ=255)
                                             cGACCAGGCCAATACGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCATTTCACTGTTGCATACAAGGATAGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCACGGCAAAACTTACCGGCATCATCAGAc                                                                                                     >  2:892257/1‑150 (MQ=255)
                                              gACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACg                                                                                                    <  1:1248557/150‑1 (MQ=255)
                                                ccAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTg                                                                                                  >  1:846523/1‑150 (MQ=255)
                                                 cAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGa                                                                                                 >  2:735760/1‑150 (MQ=255)
                                                   ggCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACg                                                                                               >  1:76502/1‑150 (MQ=255)
                                                    gCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACgg                                                                                              <  2:688046/150‑1 (MQ=255)
                                                     ccAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGc                                                                                             <  2:602184/150‑1 (MQ=255)
                                                     ccAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGc                                                                                             >  2:628020/1‑150 (MQ=255)
                                                      cAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCg                                                                                            <  2:1149125/150‑1 (MQ=255)
                                                       aaTAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGGGCGCCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGCCAAAACTTAACGGCATCATCAGACGTGACGGCGa                                                                                           <  2:1212255/150‑1 (MQ=255)
                                                             tgtTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCTGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTg                                                                                     >  1:1063499/1‑150 (MQ=255)
                                                             tgtTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTg                                                                                     >  2:490305/1‑150 (MQ=255)
                                                             tgtTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTg                                                                                     <  1:1485870/150‑1 (MQ=255)
                                                             tggtACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTg                                                                                     <  1:1492761/147‑1 (MQ=255)
                                                              gtTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGc                                                                                    >  1:165360/1‑150 (MQ=255)
                                                               ttACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCa                                                                                   <  1:66530/150‑1 (MQ=255)
                                                                tACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCAt                                                                                  <  2:962501/150‑1 (MQ=255)
                                                                    cTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGc                                                                              >  2:238409/1‑150 (MQ=255)
                                                                            aCTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAAc                                                                      >  1:697624/1‑150 (MQ=255)
                                                                                 aGCCGTGATAGCCTTCACGGAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGTCGTGACGGCGACGTTTGCATACGCATGGCAACGCTTa                                                                 <  2:828638/150‑1 (MQ=255)
                                                                                 aGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTa                                                                 <  1:1482147/150‑1 (MQ=255)
                                                                                 aGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTa                                                                 >  2:1874071/1‑150 (MQ=255)
                                                                                  gCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTaa                                                                <  1:1497406/150‑1 (MQ=255)
                                                                                  gCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTaa                                                                <  1:1504268/150‑1 (MQ=255)
                                                                                    cGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAaca                                                              <  1:924254/150‑1 (MQ=255)
                                                                                     gTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAacac                                                             <  1:735760/150‑1 (MQ=255)
                                                                                      tGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAAcacc                                                            <  1:1920121/150‑1 (MQ=255)
                                                                                        aTAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCCGCTTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAAcaccac                                                          <  2:1620543/150‑1 (MQ=255)
                                                                                           gCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATg                                                                           >  1:1751165/1‑130 (MQ=255)
                                                                                           gCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATg                                                                           <  2:1751165/130‑1 (MQ=255)
                                                                                              ttCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGc                                                                                    <  1:1823409/118‑1 (MQ=255)
                                                                                              ttCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGc                                                                                    >  2:1823409/1‑118 (MQ=255)
                                                                                              ttCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAAt                                                    <  1:564725/150‑1 (MQ=255)
                                                                                                    cagcaATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCCCGGGAATAAAGAt                                              >  1:1280514/1‑150 (MQ=255)
                                                                                                    cagcaATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGAt                                              >  2:343752/1‑150 (MQ=255)
                                                                                                       caATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGc                                           <  1:1035930/150‑1 (MQ=255)
                                                                                                           tCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACa                                       <  1:1447528/150‑1 (MQ=255)
                                                                                                             aCTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAAc                                     >  2:111610/1‑150 (MQ=255)
                                                                                                                  tGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCCGCCCGGCAAAACTTAACGGCCTCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCgg                                >  2:652140/1‑150 (MQ=255)
                                                                                                                  tGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCgg                                >  2:1016469/1‑150 (MQ=255)
                                                                                                                  tGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACCTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGCTAGCGACAACGCCgg                                >  1:604314/1‑150 (MQ=255)
                                                                                                                   gACTACCAGGTTCGGCGGCGTCGCCACCAGCGTAATCATGCCGCTAATCAGACCGTCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGt                               >  2:730099/1‑150 (MQ=255)
                                                                                                                    aCTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCCCGGGAATAAAGATAGCGACAACGCCGGtt                              >  1:855011/1‑150 (MQ=255)
                                                                                                                     cTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTg                             <  2:126542/150‑1 (MQ=255)
                                                                                                                     cTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTg                             >  1:1698870/1‑150 (MQ=255)
                                                                                                                        ccAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTTACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAAc                          >  1:1356616/1‑150 (MQ=255)
                                                                                                                        ccAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAAc                          <  2:165360/150‑1 (MQ=255)
                                                                                                                         cAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACt                         >  1:298743/1‑150 (MQ=255)
                                                                                                                         cAGGTTCGGCGGCGTCGCCACCAGCGGCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACt                         <  1:871435/150‑1 (MQ=255)
                                                                                                                            gTTCGGCGGCGTCGCCACCAGCGTCATCATGCTGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCAt                      <  2:1063499/150‑1 (MQ=255)
                                                                                                                            gTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCAt                      <  1:952107/150‑1 (MQ=255)
                                                                                                                             ttCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATa                     <  1:171384/150‑1 (MQ=255)
                                                                                                                                     cgTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACgcgct             <  1:1874071/150‑2 (MQ=255)
                                                                                                                                     cgTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGcc             >  1:1914448/1‑150 (MQ=255)
                                                                                                                                       tCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGTCAAAACTTAACGGCATCATCAGACGTTACTTCGACGTTTGCATACGCATGGCAACGCTTAACACCACTGGAATAAAGATAGCGACAACGCCGGTTGAACTAATAAACGCGCCaa           <  2:1275563/150‑1 (MQ=255)
                                                                                                                                          ccaccaGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGcc        <  1:177698/150‑1 (MQ=255)
                                                                                                                                           gattaGCGGCATGATGACGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGccc       >  2:459526/5‑150 (MQ=255)
                                                                                                                                           cattaGCGGCATGATGACGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGccc       >  1:1640551/5‑150 (MQ=255)
                                                                                                                                              taGCGGCATGATGACGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCcgc    >  2:1441398/2‑150 (MQ=255)
                                                                                                                                                gCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGa  >  1:616939/1‑150 (MQ=255)
                                                                                                                                                gCGGCATGATGACGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGa  <  2:1640551/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCATGAAACGCATCACTAACATATACAAAATACCCAGCACCAGCACGACCAGGCCAATAGGTGTTACGCTAAAGAAACTGAAGCCGTGATAGCCTTCACGCAGCAATTCACTGTTGACTACCAGGTTCGGCGGCGTCGCCACCAGCGTCATCATGCCGCTAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGA  >  NC_000913/2410755‑2411048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑