BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,820,248	Δ1 bp	6.8%	coding (923/1683 nt)	ligB ←	DNA ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,820,248	0	A	.	6.8%	531.8 / 28.8	161	coding (923/1683 nt)	ligB	DNA ligase B
Reads supporting (aligned to +/- strand): ref base A (85/65); new base . (4/7); total (89/72)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 165 total aligned reads displayed.

CCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCA  >  NC_000913/3820099‑3820397

cccACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTa                                                                                                                                                       >  2:28647/1‑150 (MQ=255)

  cACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACa                                                                                                                                                     >  2:491754/1‑150 (MQ=255)

     tCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGt                                                                                                                                                  >  2:1262171/1‑150 (MQ=255)

         gCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGTAGCT‑CAGGTTgcc                                                                                                                                              <  1:1195181/150‑3 (MQ=255)

          ccAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGTAGCT‑CAGGTTgccg                                                                                                                                             <  1:974546/150‑4 (MQ=255)

            aGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGTAGCT‑CAGGTTgccgaa                                                                                                                                           <  1:1310244/150‑6 (MQ=255)

               gcCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGGGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTcc                                                                                                                                        <  1:574749/150‑1 (MQ=255)

                ccTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCa                                                                                                                                       <  1:276702/150‑1 (MQ=255)

                 cTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAg                                                                                                                                      <  1:491754/150‑1 (MQ=255)

                   gACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGc                                                                                                                                    >  1:236195/1‑150 (MQ=255)

                     cGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGGGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGcca                                                                                                                                  <  1:90783/150‑1 (MQ=255)

                          ccAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGTAGCT‑CAGGTTgcc                                                                                                                                              >  2:1074661/1‑131 (MQ=255)

                           cAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGcc                                                                                                                            >  2:27934/1‑150 (MQ=255)

                           cAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGcc                                                                                                                            <  2:1201692/150‑1 (MQ=255)

                            aaTATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCa                                                                                                                           <  2:1068230/150‑1 (MQ=255)

                            aaTATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCa                                                                                                                           >  2:438918/1‑150 (MQ=255)

                            aaTATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCa                                                                                                                           >  2:781799/1‑150 (MQ=255)

                             atatTCACCCGCTGGGCTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCAc                                                                                                                          >  2:728050/1‑150 (MQ=255)

                             atatTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCAc                                                                                                                          <  1:10187/150‑1 (MQ=255)

                                  cACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCCCCAGCCACTCTGc                                                                                                                     >  2:1367174/1‑150 (MQ=255)

                                   aCCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGcc                                                                                                                    <  2:1187011/150‑1 (MQ=255)

                                   aCCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGcc                                                                                                                    <  1:1426528/150‑1 (MQ=255)

                                     ccGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTg                                                                                                                  <  1:1173369/150‑1 (MQ=255)

                                      cGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGc                                                                                                                             >  1:486370/1‑138 (MQ=255)

                                            aCTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCActc                                                                                                                        <  2:17477/137‑1 (MQ=255)

                                            aCTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGt                                                                                                           >  2:414566/1‑150 (MQ=255)

                                             cTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTa                                                                                                          >  2:382692/1‑150 (MQ=255)

                                             cTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTa                                                                                                          >  1:742708/1‑150 (MQ=255)

                                             cTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTa                                                                                                          <  2:1030431/150‑1 (MQ=255)

                                              ttttttATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAg                                                                                                         <  1:763227/150‑1 (MQ=255)

                                                ttttATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGcc                                                                                                       >  1:960302/1‑150 (MQ=255)

                                                ttttATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGcc                                                                                                       >  1:855142/1‑150 (MQ=255)

                                                      catcCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGc                                                                                                 >  2:532825/1‑150 (MQ=255)

                                                        tcCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCgg                                                                                               >  1:946965/1‑150 (MQ=255)

                                                           aGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGAt                                                                                            >  1:448887/1‑150 (MQ=255)

                                                           aGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGAt                                                                                            >  2:1211345/1‑150 (MQ=255)

                                                               tGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTACCTGGCCCGGTAGCCAATGGCGGGATTCTg                                                                                        >  2:1109399/1‑150 (MQ=255)

                                                                 aCAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGc                                                                                      >  2:323542/1‑150 (MQ=255)

                                                                 aCAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGc                                                                                      <  2:258218/150‑1 (MQ=255)

                                                                  cAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGct                                                                                     >  2:977833/1‑150 (MQ=255)

                                                                       gCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTc                                                                                >  2:85808/1‑150 (MQ=255)

                                                                          aGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTcgcc                                                                             >  1:1112101/1‑150 (MQ=255)

                                                                           gTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTcgccg                                                                            <  2:589103/150‑1 (MQ=255)

                                                                           gTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTcgccg                                                                            >  1:366370/1‑150 (MQ=255)

                                                                              aCGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCAGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTc                                                                         >  2:1238343/1‑150 (MQ=255)

                                                                               cGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCg                                                                        >  1:1259296/1‑150 (MQ=255)

                                                                                    ccaccGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTATCGCCGCTCGTACaa                                                                   >  2:856141/1‑150 (MQ=255)

                                                                                      accGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACt                                                                 >  1:971378/1‑150 (MQ=255)

                                                                                       ccGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTAc                                                                     >  2:1281443/1‑145 (MQ=255)

                                                                                       ccGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTAc                                                                     <  1:1281443/145‑1 (MQ=255)

                                                                                       ccGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTa                                                                >  2:336932/1‑150 (MQ=255)

                                                                                       ccGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTCCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTa                                                                >  1:1362941/1‑150 (MQ=255)

                                                                                               ttACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCg                                                        >  1:821345/1‑150 (MQ=255)

                                                                                                tACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCgg                                                       <  1:590413/150‑1 (MQ=255)

                                                                                                   cGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGa                                                    <  1:1405188/150‑1 (MQ=255)

                                                                                                    gCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGAc                                                   <  2:414192/150‑1 (MQ=255)

                                                                                                     ctctTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTTCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACg                                                  >  1:114957/1‑150 (MQ=255)

                                                                                                      tctTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGa                                                 >  1:68741/1‑150 (MQ=255)

                                                                                                         tACCCACCGCACACTGATTTGCCTTCACTTCGGCAAGCTT‑ATCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTTCCTGGCCCGTTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAgg                                              <  2:494200/150‑1 (MQ=255)

                                                                                                         tACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAgg                                              <  2:660127/150‑1 (MQ=255)

                                                                                                          aCCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAggg                                             >  2:1299928/1‑150 (MQ=255)

                                                                                                          aCCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAggg                                             <  1:85808/150‑1 (MQ=255)

                                                                                                           cccACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGt                                            <  1:1262171/150‑1 (MQ=255)

                                                                                                           cccACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGt                                            >  1:584392/1‑150 (MQ=255)

                                                                                                              aCCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAAt                                         >  2:179778/1‑150 (MQ=255)

                                                                                                              aCCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAAt                                         >  2:356601/1‑150 (MQ=255)

                                                                                                              aCCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAAt                                         <  1:831133/150‑1 (MQ=255)

                                                                                                                 gCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTTCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTcc                                      <  2:114957/150‑1 (MQ=255)

                                                                                                                 gCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTcc                                      <  1:532825/150‑1 (MQ=255)

                                                                                                                   aaaCTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGc                                    <  1:1199365/150‑1 (MQ=255)

                                                                                                                   aaaCTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGc                                    >  2:924312/1‑150 (MQ=255)

                                                                                                                    aaCTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCt                                   <  1:728050/150‑1 (MQ=255)

                                                                                                                     aCTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCtt                                  >  1:1388010/1‑150 (MQ=255)

                                                                                                                     aCTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCtt                                  <  1:438918/150‑1 (MQ=255)

                                                                                                                      cTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCttt                                 >  2:929578/1‑150 (MQ=255)

                                                                                                                        gAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTcc                               >  1:968248/1‑150 (MQ=255)

                                                                                                                        gAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTcc                               <  2:14595/150‑1 (MQ=255)

                                                                                                                             gCCTTCCCTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCCCCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCccccac                          >  2:206987/1‑150 (MQ=255)

                                                                                                                             gCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCcaccac                          >  1:658772/1‑150 (MQ=255)

                                                                                                                             gCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCcaccac                          <  2:1414460/150‑1 (MQ=255)

                                                                                                                             gCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGAGGGATTCTGGCTCATTCGCCGCTCGTACAACTAAGACATCTGTGACGCAGGGTAATTCCGCTTTccaccaa                          >  2:212819/1‑149 (MQ=255)

                                                                                                                               cTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTc                        >  1:708808/1‑150 (MQ=255)

                                                                                                                                ttCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCa                       >  2:464265/1‑150 (MQ=255)

                                                                                                                                ttCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCa                       >  1:213046/1‑150 (MQ=255)

                                                                                                                                 tCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCAt                      <  2:575198/150‑1 (MQ=255)

                                                                                                                                  cACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCAtt                     >  2:851913/1‑150 (MQ=255)

                                                                                                                                   aCTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTg                    <  1:28647/150‑1 (MQ=255)

                                                                                                                                   aCTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTg                    <  1:1422575/150‑1 (MQ=255)

                                                                                                                                   aCTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTg                    >  1:299426/1‑150 (MQ=255)

                                                                                                                                     ttCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGTCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCg                  >  2:747307/1‑150 (MQ=255)

                                                                                                                                      tCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCAGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGt                 >  2:694232/1‑150 (MQ=255)

                                                                                                                                        ggCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTAc               >  2:590661/1‑150 (MQ=255)

                                                                                                                                        ggCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTAc               >  2:472791/1‑150 (MQ=255)

                                                                                                                                         gCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTAcg              >  2:883984/1‑150 (MQ=255)

                                                                                                                                         gCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTAcg              >  2:1286548/1‑150 (MQ=255)

                                                                                                                                              cTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACgcgcgc         <  1:10646/150‑1 (MQ=255)

                                                                                                                                               tG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCa        <  1:464265/150‑1 (MQ=255)

                                                                                                                                               tG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCa        <  1:132051/150‑1 (MQ=255)

                                                                                                                                                  aGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCTGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAAc      <  1:1238343/150‑1 (MQ=255)

                                                                                                                                                      aCAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACt     <  1:1296358/147‑1 (MQ=255)

CCCACTCCTGCCAGCGCCTGACGGAACCAATATTCACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTTACCGCTCTTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTG‑AGCTACAGGTTGATATTTCCAGGCCACCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCGTACGCGCGCAACTTCA  >  NC_000913/3820099‑3820397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`