Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	897,973	Δ1 bp	13.0%	intergenic (+44/‑16)	potI → / → ybjO	putrescine ABC transporter membrane subunit PotI/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	897,973	0	T	.	13.0%	205.2 / 25.3	69	intergenic (+44/‑16)	potI/ybjO	putrescine ABC transporter membrane subunit PotI/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (31/28);  new base . (5/4);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

TCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATTTTC  >  NC_000913/897829‑898120                                                                                                                                                  |                                                                                                                                                    tCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCgg                                                                                                                                                <  1:256991/150‑1 (MQ=255)  ccTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGc                                                                                                                                               >  2:880509/1‑150 (MQ=255)     cGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATG‑CGCGGCAtt                                                                                                                                            <  2:644793/150‑1 (MQ=255)     cGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATG‑CGCGGCAtt                                                                                                                                            <  1:1278629/150‑1 (MQ=255)         gcgGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAATACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACTTAATGCCGCGACATG‑CGCGGCAttatgt                                                                                                                                        <  2:374334/150‑5 (MQ=255)           ggTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATG‑CGCGGCAttatgtag                                                                                                                                      <  2:1278175/150‑7 (MQ=255)           ggTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTc                                                                                                                                      <  2:254623/150‑1 (MQ=255)           ggTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTc                                                                                                                                      >  1:216263/1‑150 (MQ=255)             tCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCAt                                                                                                                                    <  1:629463/150‑1 (MQ=255)             tCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCAt                                                                                                                                    <  1:457588/150‑1 (MQ=255)                       cGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGaa                                                                                                                          >  2:646704/1‑150 (MQ=255)                             tATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTg                                                                                                                    >  2:1144934/1‑150 (MQ=255)                              aTCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTgg                                                                                                                   >  1:325624/1‑150 (MQ=255)                               tCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTggg                                                                                                                  >  1:681417/1‑150 (MQ=255)                                 gCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAt                                                                                                                >  2:520353/1‑150 (MQ=255)                                  ccTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAtt                                                                                                               >  1:251057/1‑150 (MQ=255)                                     tgTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAttttt                                                                                                            <  2:1042824/149‑1 (MQ=255)                                      gTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAtttttt                                                                                                           >  2:1084738/1‑150 (MQ=255)                                       tATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTa                                                                                                          >  1:1366619/1‑150 (MQ=255)                                         tCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAg                                                                                                        <  2:96044/150‑1 (MQ=255)                                                 cTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATc                                                                                                >  1:702011/1‑150 (MQ=255)                                                    gcgcAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTc                                                                                             <  1:665696/150‑1 (MQ=255)                                                       cAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCAtc                                                                                          >  1:811144/1‑150 (MQ=255)                                                       cAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGCCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCAtc                                                                                          >  2:113634/1‑150 (MQ=255)                                                          aaaaaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCa                                                                                       <  1:1021595/150‑1 (MQ=255)                                                           aaaaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCAt                                                                                      >  2:468769/1‑150 (MQ=255)                                                           aaaaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCAt                                                                                      >  2:752030/1‑150 (MQ=255)                                                                         gcgACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAAtgt                                                                        >  2:1233193/1‑150 (MQ=255)                                                                         gcgACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAAtgt                                                                        >  1:545086/1‑150 (MQ=255)                                                                         gcgACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAAtgt                                                                        <  1:994504/150‑1 (MQ=255)                                                                            aCATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGcc                                                                     <  2:1115949/150‑1 (MQ=255)                                                                            aCATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTATTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGcc                                                                     <  1:633648/150‑1 (MQ=255)                                                                              aTCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTg                                                                   <  1:520353/150‑1 (MQ=255)                                                                                ccAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTgcg                                                                 >  2:1383644/1‑150 (MQ=255)                                                                                ccAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTgcg                                                                 <  2:406076/150‑1 (MQ=255)                                                                                        cAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAATCGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCa                                                         <  1:853116/150‑1 (MQ=255)                                                                                        cAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCa                                                         <  2:325624/150‑1 (MQ=255)                                                                                          aGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAgg                                                       >  2:958201/1‑150 (MQ=255)                                                                                          aGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAgg                                                       <  1:638969/150‑1 (MQ=255)                                                                                             cGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTgg                                                    >  2:380356/1‑150 (MQ=255)                                                                                               tGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCg                                                                                 >  2:814209/1‑119 (MQ=255)                                                                                               tGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCg                                                                                 >  2:809138/1‑119 (MQ=255)                                                                                               tGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCg                                                                                 <  1:814209/119‑1 (MQ=255)                                                                                               tGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCg                                                                                 <  1:809138/119‑1 (MQ=255)                                                                                                ggCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTggcgg                                                 >  2:1217030/1‑150 (MQ=255)                                                                                                ggCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTggcgg                                                 <  1:468769/150‑1 (MQ=255)                                                                                                  cTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGgcggcg                                               >  1:1213462/1‑150 (MQ=255)                                                                                                     aaGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTc                                            <  1:1144934/150‑1 (MQ=255)                                                                                                          acTAAAATTTGCCAACCTGGCTACATAATGCCGCA‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCat                                       <  1:257376/150‑1 (MQ=255)                                                                                                           cTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAgg                                                       <  1:562479/133‑1 (MQ=255)                                                                                                           cTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAgg                                                       >  2:562479/1‑133 (MQ=255)                                                                                                           cTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCatt                                      >  1:213816/1‑150 (MQ=255)                                                                                                             aaaaTTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCattat                                    >  1:63259/1‑150 (MQ=255)                                                                                                                    gCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATc                             >  1:1279106/1‑150 (MQ=255)                                                                                                                     ccAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATcc                            >  1:1407287/1‑150 (MQ=255)                                                                                                                        aCCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTg                         <  2:749045/150‑1 (MQ=255)                                                                                                                               tACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGa                  >  2:1405568/1‑150 (MQ=255)                                                                                                                                 cATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACg                <  1:1084738/150‑1 (MQ=255)                                                                                                                                   caaTGCCGCGACATG‑CGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTg              >  2:1080835/2‑150 (MQ=255)                                                                                                                                   caaTGCCGCGACATG‑CGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTg              >  1:1150962/2‑150 (MQ=255)                                                                                                                                    aaTGCCGCGACATG‑CGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGc             >  2:719569/1‑150 (MQ=255)                                                                                                                                    aaTGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGc             <  2:681417/150‑1 (MQ=255)                                                                                                                                    aaTGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGc             <  1:1405568/150‑1 (MQ=255)                                                                                                                                     aTGCCGCGACATG‑CGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCt            >  2:840763/1‑150 (MQ=255)                                                                                                                                      tGCCGCGACATG‑CGCGGCATTGTTTTCATGGAAGACGAAACGTAGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtg           >  2:270736/1‑150 (MQ=255)                                                                                                                                      tGCCGAG‑CAAGACGCGGAATTGTTTTCATGGAAGACCAAACGTTGGCATTTTTTAAGAAAACATCTTCATATCATGCTCCCCTGAATGTGCAAGAGATGGTGCAGGTCGCGGCGCTCGACATTATTAAGAAACTTGGCCTCGACGTGcag           >  2:1072588/1‑148 (MQ=255)                                                                                                                                         cgcg‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtgatg        >  2:983981/1‑150 (MQ=255)                                                                                                                                                tGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGTTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATTTTc  <  1:646704/150‑1 (MQ=255)                                                                                                                                                tGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATTTTc  <  2:216263/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATTTTC  >  NC_000913/897829‑898120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑