| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,085 | Δ1 bp | 100% | coding (2069/2664 nt) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | . | 100.0% | 259.1 / NA | 57 | coding (2069/2664 nt) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (32/25); total (32/25) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGT > NC_000913/124945‑125227 | tGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTg > 2:470067/1‑150 (MQ=255) gTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGaa > 2:404999/1‑150 (MQ=255) tCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAAc < 1:1296064/150‑1 (MQ=255) gCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGaaaa > 1:1294544/1‑150 (MQ=255) ccACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAAc < 2:1211596/150‑1 (MQ=255) aTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTAcc > 2:1203595/1‑150 (MQ=255) ttCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACca < 2:1197836/150‑1 (MQ=255) tCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACcac < 2:1270154/150‑1 (MQ=255) cAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTAAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACcaca > 2:265037/1‑150 (MQ=255) cTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCgg > 2:376012/1‑150 (MQ=255) cTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCgg < 1:636823/150‑1 (MQ=255) gACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCa > 2:795015/1‑150 (MQ=255) aCTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCaa > 2:84064/1‑150 (MQ=255) tATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATg < 2:1294544/150‑1 (MQ=255) cccGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCg > 1:1008147/1‑150 (MQ=255) ccGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCgg > 1:102055/1‑150 (MQ=255) aaCTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTTAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAg < 1:265037/150‑1 (MQ=255) aCTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAgg < 1:1126926/150‑1 (MQ=255) aCTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAgg < 1:78139/150‑1 (MQ=255) cTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGt > 2:749110/1‑150 (MQ=255) tATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCt > 1:198643/1‑150 (MQ=255) tctTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAgg < 2:280918/150‑1 (MQ=255) aCGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAgg < 2:808227/150‑1 (MQ=255) aCCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTAt > 1:180807/1‑150 (MQ=255) gcttacgcttacGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTa < 1:376012/150‑1 (MQ=255) cttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTAt > 2:249526/1‑150 (MQ=255) tacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCt > 2:574482/1‑150 (MQ=255) tacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCt > 2:780112/1‑150 (MQ=255) acgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTa < 2:718114/150‑1 (MQ=255) cgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTAc > 1:282478/1‑150 (MQ=255) aaGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCGGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaa < 2:203960/150‑1 (MQ=255) ttGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACt < 2:956213/150‑1 (MQ=255) cTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGa < 1:249526/150‑1 (MQ=255) gTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa > 2:526347/1‑150 (MQ=255) tcatcaTGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAAc < 2:1127106/150‑1 (MQ=255) catgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAtt > 2:1279123/1‑150 (MQ=255) atgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTg < 1:1279123/150‑1 (MQ=255) tgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGa > 2:715510/1‑150 (MQ=255) gcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGaa > 1:625194/1‑150 (MQ=255) gACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTa > 1:156180/1‑150 (MQ=255) ggTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCa < 1:991496/150‑1 (MQ=255) tGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAgg > 2:198075/1‑150 (MQ=255) tGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAgg > 2:203224/1‑150 (MQ=255) ggAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCa > 2:220357/1‑78 (MQ=255) ggAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCa < 1:220357/78‑1 (MQ=255) cGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAg < 2:180807/150‑1 (MQ=255) gTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGt < 2:686494/150‑1 (MQ=255) cGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGt > 2:1028095/1‑129 (MQ=255) cGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGt < 1:1028095/129‑1 (MQ=255) cGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgctg > 1:819380/1‑150 (MQ=255) cGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgctg > 1:800504/1‑150 (MQ=255) ggTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgctgc > 1:320124/1‑150 (MQ=255) gTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgctgct > 1:115735/1‑150 (MQ=255) aaGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCgg < 1:470067/150‑1 (MQ=255) gagaACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGtt > 1:492635/1‑150 (MQ=255) cGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCTGGTTCTATc < 1:749110/150‑1 (MQ=255) ctactacA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGctt > 2:1363658/1‑148 (MQ=255) | TGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGT > NC_000913/124945‑125227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |