Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,325,042	Δ1 bp	10.9%	coding (287/1323 nt)	atoE →	short chain fatty acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,325,042	0	A	.	10.9%	199.2 / 17.3	64	coding (287/1323 nt)	atoE	short chain fatty acid transporter
Reads supporting (aligned to +/- strand):  ref base A (27/30);  new base . (3/4);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGAAAATGTGGGGTGACGGTTTCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGCTCATTGCCTGCGCCTACATT  >  NC_000913/2324893‑2325190                                                                                                                                                       |                                                                                                                                                     ggTGAAAATGTGGGGTGACGGTTTCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACa                                                                                                                                                      <  1:1295752/150‑1 (MQ=255)            ggggTGACGGTTTCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGc                                                                                                                                           <  2:332538/150‑1 (MQ=255)             gggTGACGGTTTCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCCTGTAC‑GGGCGTctttgc                                                                                                                                          <  2:1166389/150‑6 (MQ=255)             gggTGACGGTTTCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCCTGTAC‑GGGCGTctttgc                                                                                                                                          <  1:123260/150‑6 (MQ=255)              ggTGACGGTTTCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTg                                                                                                                                         <  1:528186/150‑1 (MQ=255)               gTGACGGTTTCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCCTGTAC‑GGGCGTctttgcgg                                                                                                                                        <  1:639405/150‑8 (MQ=255)                tGACGGTTTCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGt                                                                                                                                       <  1:208464/150‑1 (MQ=255)                 gACGGTTTCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTc                                                                                                                                      >  2:330914/1‑150 (MQ=255)                    ggTTTCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACt                                                                                                                                   >  1:235564/1‑150 (MQ=255)                        tCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTttct                                                                                                                               >  1:853770/1‑150 (MQ=255)                           ggAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCg                                                                                                                            <  1:130982/150‑1 (MQ=255)                                   cTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTc                                                                                                                                      >  1:320911/1‑132 (MQ=255)                                   cTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTc                                                                                                                                      <  2:320911/132‑1 (MQ=255)                                      gCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCt                                                                                                                 <  1:1017887/150‑1 (MQ=255)                                      gCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCt                                                                                                                 <  2:419154/150‑1 (MQ=255)                                       cGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTGGGTTCAGTCGCtt                                                                                                                <  2:645536/150‑1 (MQ=255)                                        gTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTtg                                                                                                               <  2:108120/150‑1 (MQ=255)                                                tGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGtcatca                                                                                                       <  1:1427510/150‑1 (MQ=255)                                                 gCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCaa                                                                                                      >  1:994997/1‑150 (MQ=255)                                                     aTGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTgg                                                                                                  <  1:623407/150‑1 (MQ=255)                                                       ggCGCTTATCATCGTTACCGGTCATGCCCTTGCCTGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTgggg                                                                                                <  2:311175/150‑1 (MQ=255)                                                       ggCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTgggg                                                                                                <  2:923047/150‑1 (MQ=255)                                                       ggCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCCCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTgggg                                                                                                <  2:732057/150‑1 (MQ=255)                                                                catcGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGAtttggttt                                                                                       >  1:1135262/1‑150 (MQ=255)                                                                catcGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTAGTCTCATCAACTGGGGAtttggttt                                                                                       >  2:1396190/1‑150 (MQ=255)                                                                 atcGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGAtttggtttg                                                                                      <  1:95938/150‑1 (MQ=255)                                                                 atcGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGAtttggtttg                                                                                      <  1:920962/150‑1 (MQ=255)                                                                 atcGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGAtttggtttg                                                                                      >  1:571606/1‑150 (MQ=255)                                                                  tcGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTAGAGGGCGTCATGCTGGTCACTTTCTTCGGTTCATTCGCTTGTGTCATCAACTGGGGAtttggttttg                                                                                     <  2:1245378/150‑3 (MQ=255)                                                                  tcGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGAtttggtttgg                                                                                     >  1:1277903/1‑150 (MQ=255)                                                                  tcGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGAtttggtttgg                                                                                     <  1:330914/150‑1 (MQ=255)                                                                   cGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATttggtttggt                                                                                    <  1:188230/150‑1 (MQ=255)                                                                   cGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATttggtttggt                                                                                    >  2:1122983/1‑150 (MQ=255)                                                                        ccGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCg                                                                               >  1:170037/1‑150 (MQ=255)                                                                        ccGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCg                                                                               <  2:853770/150‑1 (MQ=255)                                                                         cGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCgg                                                                              >  1:134935/1‑150 (MQ=255)                                                                               tGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCCTGTAC‑GGGCGTctttg                                                                                                                                           >  1:577537/1‑79 (MQ=38)                                                                               tGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCCTGTAC‑GGGCGTctttg                                                                                                                                           <  2:577537/83‑5 (MQ=38)                                                                               tGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCGCTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAAt                                                                        >  2:1147761/1‑150 (MQ=255)                                                                                 cccTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTATCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGt                                                                      >  2:193514/1‑150 (MQ=255)                                                                                       ccAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGccc                                                                <  1:664922/150‑1 (MQ=255)                                                                                            tctGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCTGCGCAATGTTTGCCCGTGaa                                                           <  1:572724/150‑1 (MQ=255)                                                                                             ctGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGTGTATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAg                                                          <  2:542562/150‑1 (MQ=255)                                                                                             ctGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAg                                                          <  2:1358407/150‑1 (MQ=255)                                                                                                 tCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGCCGc                                                      >  2:751759/1‑150 (MQ=255)                                                                                                     gTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGccccg                                                  >  2:178277/1‑148 (MQ=255)                                                                                                           aGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCCTCAACTGCGGATTTGGTTTGGTTCTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTc                                            >  2:223541/1‑150 (MQ=255)                                                                                                              ttGCTGCGTTCTGGCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGTCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTcccc                                         <  1:642783/150‑1 (MQ=255)                                                                                                                     gTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTg                                  >  2:1029741/1‑150 (MQ=255)                                                                                                                            cgccTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATcc                           >  2:570809/1‑150 (MQ=255)                                                                                                                                 ccgccgCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGc                      >  1:280278/1‑150 (MQ=255)                                                                                                                                 ccgccgCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGc                      >  1:728514/1‑150 (MQ=255)                                                                                                                                   gccgcAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGCTc                    >  2:1223731/1‑150 (MQ=255)                                                                                                                                       gcatgACGCCCTGTAC‑GGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGCTCATTg                >  2:341786/5‑150 (MQ=255)                                                                                                                                        catgACGCCCTGTAC‑GGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGCCGAGTCCCCGGTTCTGATTATCCGTTGCTCATTGc               >  2:122231/4‑150 (MQ=255)                                                                                                                                           gACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATttggtttggt                                                                                    <  1:170519/78‑1 (MQ=255)                                                                                                                                           gACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATttggtttggt                                                                                    >  2:170519/1‑78 (MQ=255)                                                                                                                                             cGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGCTCATTGCCTgcg          <  1:1122983/150‑1 (MQ=255)                                                                                                                                             cGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGCTCATTGCCTgcg          >  1:819481/1‑150 (MQ=255)                                                                                                                                               ccc‑GTACAGGGCGTCATGCTTGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCTCAATGTTTGCCCGTGAAGTGGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGCTCATTGCCTGCGcc        <  2:929777/150‑1 (MQ=255)                                                                                                                                                cc‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGa                                 <  1:1058121/124‑1 (MQ=255)                                                                                                                                                cc‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGa                                 >  2:1058121/1‑124 (MQ=255)                                                                                                                                                 c‑GTACAGGGCGTCATGCTGGTCGCTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGCTCATTGCCTGCGCCTa      <  1:1147761/150‑1 (MQ=255)                                                                                                                                                 c‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGCTCATTGCCTGCGCCTa      >  2:95710/1‑150 (MQ=255)                                                                                                                                                      cAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGCTCATTGCCTGCGCCTACAtt  >  2:1020966/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                     GGTGAAAATGTGGGGTGACGGTTTCTGGAACTTGCTGGCGTTTGGTATGCAGATGGCGCTTATCATCGTTACCGGTCATGCCCTTGCCAGCTCTGCTCCGGTGAAAAGTTTGCTGCGTACTGCCGCCTCCGCCGCAAAGACGCCC‑GTACAGGGCGTCATGCTGGTCACTTTCTTCGGTTCAGTCGCTTGTGTCATCAACTGGGGATTTGGTTTGGTTGTCGGCGCAATGTTTGCCCGTGAAGTCGCCCGGCGAGTCCCCGGTTCTGATTATCCGTTGCTCATTGCCTGCGCCTACATT  >  NC_000913/2324893‑2325190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑