Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,674,183:1 +G 11.4% coding (271/1035 nt) tqsA → autoinducer 2 exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,1831.G11.4% 243.6 / 33.1 70coding (271/1035 nt)tqsAautoinducer 2 exporter
Reads supporting (aligned to +/- strand):  ref base . (34/28);  new base G (4/4);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTT  >  NC_000913/1674038‑1674331
                                                                                                                                                  |                                                                                                                                                    
tattGCTGTTATTCTTATCCCGCTGGTGCAACACATGGTCCGCTGTCGTGTGCCGCGGGTACTTGCGGTGTCGATTTTGATGACCATCATCGTTATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑cgcg                                                                                                                                                  <  2:122418/150‑1 (MQ=255)
tattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑cgcg                                                                                                                                                  <  2:1108160/150‑1 (MQ=255)
tattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCG‑TCAACTCGTTGagcgcg                                                                                                                                                  <  1:1155049/150‑6 (MQ=255)
 attGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCG‑TCAACTCGTTGAGCGCgg                                                                                                                                                 <  2:954647/150‑1 (MQ=255)
 attGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTACGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCgg                                                                                                                                                 <  2:619703/150‑1 (MQ=255)
  ttGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCG‑TCAACTCGTTGAGCGCGGa                                                                                                                                                <  1:1259125/150‑1 (MQ=255)
    gCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACg                                                                                                                                              >  1:1396821/1‑150 (MQ=255)
    gCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCG‑TCAACTCGTTGAGCGCGGaac                                                                                                                                              <  1:88702/150‑3 (MQ=255)
     cTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGt                                                                                                                                             >  1:844198/1‑150 (MQ=255)
         tATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTAcc                                                                                                                                         <  1:37878/150‑1 (MQ=255)
                 aCCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATc                                                                                                                                 >  1:49502/1‑150 (MQ=255)
                      cTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAAc                                                                                                                            >  1:1125584/1‑150 (MQ=255)
                        ggTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAActc                                                                                                                          >  2:1043415/1‑150 (MQ=255)
                                      tCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGAcgccgc                                                                                                            <  1:1250521/150‑1 (MQ=255)
                                        cGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTGGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTg                                                                                                          <  2:1003711/150‑1 (MQ=255)
                                         gCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATCGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGc                                                                                                         <  2:118778/150‑1 (MQ=255)
                                          cTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCa                                                                                                        <  1:127949/150‑1 (MQ=255)
                                             gCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAgc                                                                                                     >  1:1232386/1‑150 (MQ=255)
                                             gCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAgc                                                                                                     <  2:612638/150‑1 (MQ=255)
                                                tgtgCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCt                                                                                                  >  1:197233/1‑150 (MQ=255)
                                                  tgCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTg                                                                                                <  2:548243/150‑1 (MQ=255)
                                                  tgCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTg                                                                                                <  2:48601/150‑1 (MQ=255)
                                                   gCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGa                                                                                               >  1:796396/1‑150 (MQ=255)
                                                        gtgtACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTCCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCgt                                                                                          <  1:260124/150‑1 (MQ=255)
                                                         tgtACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCgtt                                                                                         >  1:523285/1‑150 (MQ=255)
                                                             cTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGttgttg                                                                                     <  2:908427/150‑1 (MQ=255)
                                                             cTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGttgttg                                                                                     <  1:1287868/150‑1 (MQ=255)
                                                               ggCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCa                                                                                   >  1:787868/1‑150 (MQ=255)
                                                               ggCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCa                                                                                   >  1:945232/1‑150 (MQ=255)
                                                               ggCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCa                                                                                   >  1:1366074/1‑150 (MQ=255)
                                                                  ggTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCaa                                                                                                       >  2:1236875/1‑127 (MQ=255)
                                                                  ggTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCaa                                                                                                       >  2:1241953/1‑127 (MQ=255)
                                                                  ggTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCaa                                                                                                       <  1:1241953/127‑1 (MQ=255)
                                                                  ggTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCaa                                                                                                       <  1:1236875/127‑1 (MQ=255)
                                                                  ggTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAAcg                                                                                >  2:1044177/1‑150 (MQ=255)
                                                                     gtCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGt                                                                             >  1:907329/1‑150 (MQ=255)
                                                                        gATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAgg                                                                          >  1:562051/1‑150 (MQ=255)
                                                                         aTTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAggg                                                                         >  2:969809/1‑150 (MQ=255)
                                                                              gatgaCCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCGAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGa                                                                    >  2:1104849/1‑150 (MQ=255)
                                                                                tgaCCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACg                                                                  <  1:605496/150‑1 (MQ=255)
                                                                                  aCCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGtc                                                                <  2:796396/150‑1 (MQ=255)
                                                                                   ccATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGtct                                                               >  1:371293/1‑150 (MQ=255)
                                                                                       catcGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGt                                                           <  2:785513/150‑1 (MQ=255)
                                                                                       catcGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGt                                                           >  1:665358/1‑150 (MQ=255)
                                                                                          cGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGa                                                        <  2:1396821/150‑1 (MQ=255)
                                                                                            tGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGAcc                                                      >  2:1098360/1‑150 (MQ=255)
                                                                                               tGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGc                                                   <  1:969809/150‑1 (MQ=255)
                                                                                                  cGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTgg                                                >  2:682215/1‑150 (MQ=255)
                                                                                                  cGATGGTGTTGCTATTAGCTTATCTCGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTAACGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGAAGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCtcc                                                >  2:159075/1‑148 (MQ=255)
                                                                                                             cTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATAtt                                     >  1:143829/1‑150 (MQ=255)
                                                                                                              tATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTg                                    <  2:406054/150‑1 (MQ=255)
                                                                                                              tATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTg                                    <  2:401075/150‑1 (MQ=255)
                                                                                                                 tAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATc                                 >  1:887336/1‑150 (MQ=255)
                                                                                                                  aGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATcc                                >  2:182633/1‑150 (MQ=255)
                                                                                                                     ttATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGaa                             >  2:340833/1‑150 (MQ=255)
                                                                                                                       aTCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAAcg                           <  2:1125584/150‑1 (MQ=255)
                                                                                                                           gggTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACgcggc                       <  1:682215/150‑1 (MQ=255)
                                                                                                                             gTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGa                     <  2:1232386/150‑1 (MQ=255)
                                                                                                                             cgtCCGCG‑TCAACTCGTTGAGCGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGa                     >  1:1034814/3‑150 (MQ=255)
                                                                                                                              ttCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGAt                    <  1:1043415/150‑1 (MQ=255)
                                                                                                                              gtCCGCG‑TCAACTCGTTGAGCGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGAt                    >  1:1372547/2‑150 (MQ=255)
                                                                                                                                 cgcgCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGAc                 >  1:1268853/1‑150 (MQ=255)
                                                                                                                                 ccgcgtCAACTCGTTGAGCGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACgcggc                       >  2:1432785/6‑145 (MQ=255)
                                                                                                                                 ccgcgtCAACTCGTTGAGCGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACgcggc                       <  1:1432785/140‑1 (MQ=255)
                                                                                                                                  gcgcTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACg                >  1:1021073/1‑150 (MQ=255)
                                                                                                                                      tCAACTCGTTGAGCGCGGAAGTTACAGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGAAACGCGTAGGGATTGACGTCTCAGTTTACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTg             >  2:96786/1‑150 (MQ=255)
                                                                                                                                       cAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCt           >  1:1184440/1‑150 (MQ=255)
                                                                                                                                        aaCGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTc          <  2:787868/150‑1 (MQ=255)
                                                                                                                                        aaCGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTc          >  1:164422/1‑150 (MQ=255)
                                                                                                                                        aaCGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTc          >  2:1265172/1‑150 (MQ=255)
                                                                                                                                             gTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCaa     <  2:1021073/150‑1 (MQ=255)
                                                                                                                                               tGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTTTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACt   <  2:523285/150‑1 (MQ=255)
                                                                                                                                                gA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACtt  >  1:709649/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGA‑CGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCACCAACTT  >  NC_000913/1674038‑1674331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑