| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,868,865:1 | +G | 10.3% | intergenic (+23/‑90) | yeaG → / → yeaH | protein kinase YeaG/DUF444 domain‑containing protein YeaH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,868,865 | 1 | . | G | 10.3% | 206.6 / 23.3 | 58 | intergenic (+23/‑90) | yeaG/yeaH | protein kinase YeaG/DUF444 domain‑containing protein YeaH |
| Reads supporting (aligned to +/- strand): ref base . (30/22); new base G (3/3); total (33/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
AAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGG > NC_000913/1868717‑1869003 | aaaaCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGt‑t > 2:96398/1‑150 (MQ=255) aCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TAcc > 2:1027500/1‑150 (MQ=255) tCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCggg < 1:1206159/150‑1 (MQ=255) cAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGc > 1:307717/1‑150 (MQ=255) cAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCAGGCCCGGTA‑ACATGTGTACCGGGc < 2:202900/150‑1 (MQ=255) aCCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCt < 1:1422839/150‑1 (MQ=255) aCCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCt > 2:187868/1‑150 (MQ=255) ccGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCGGGccag < 2:228824/150‑3 (MQ=255) cgacgaGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTAc < 1:798796/150‑1 (MQ=255) gacgaGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACa > 1:114428/1‑150 (MQ=255) agaagaAACACGACTACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGTTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAg < 2:352668/150‑1 (MQ=255) agaagaAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAg > 2:514810/1‑150 (MQ=255) gaagaaACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGc > 1:765357/1‑150 (MQ=255) aaaCACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAAc < 2:385491/150‑1 (MQ=255) aaCACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAAcc > 2:591164/1‑150 (MQ=255) cgacgaCTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTgg < 1:241248/150‑1 (MQ=255) ttGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTgaga > 1:815952/1‑150 (MQ=255) aCCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc < 1:287925/150‑1 (MQ=255) ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCa < 1:851389/150‑1 (MQ=255) gTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAac < 2:407338/150‑1 (MQ=255) gTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAac < 1:1138256/150‑1 (MQ=255) atgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAacac > 2:60766/1‑150 (MQ=255) tgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACACCCCTGGCCCGGTACACATGT‑TACCGTGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAAcaca < 1:1351575/150‑1 (MQ=255) gatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg < 1:1143896/150‑1 (MQ=255) gatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg > 2:1298805/1‑150 (MQ=255) atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg > 1:749634/1‑150 (MQ=255) atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg < 1:902335/150‑1 (MQ=255) tgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGc > 1:973582/1‑150 (MQ=255) ggAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCg < 2:921365/150‑1 (MQ=255) gAAAAAGCCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGt > 1:578573/1‑150 (MQ=255) aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAg < 1:591164/150‑1 (MQ=255) aaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGc < 2:415838/150‑1 (MQ=255) cccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTgg > 1:86243/1‑150 (MQ=255) cccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTgg > 1:32991/1‑150 (MQ=255) ggTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtagt > 1:803967/1‑150 (MQ=255) gTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtagta < 1:309272/150‑1 (MQ=255) aCTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc < 1:60766/150‑1 (MQ=255) gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTATTACGGGGGGCata > 2:1289551/1‑150 (MQ=255) gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCata > 1:842030/1‑150 (MQ=255) tgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTgag > 2:642823/1‑96 (MQ=255) tgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTgag < 1:642823/96‑1 (MQ=255) ggTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTgg > 2:465460/1‑150 (MQ=255) tATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGtt < 1:401798/150‑1 (MQ=255) aTTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGttt > 2:1064187/1‑150 (MQ=255) gcgcGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTg < 2:114428/150‑1 (MQ=255) cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGAcc < 1:96398/150‑1 (MQ=255) cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TAACGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCACCACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGAcc > 1:556474/1‑150 (MQ=255) cgtaAATCGTCTTAACAACCCTGGCACGGAACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCg > 1:1299092/1‑150 (MQ=255) aTCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGa > 2:1305345/1‑150 (MQ=255) gTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACg > 1:220649/1‑150 (MQ=255) ttAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCa > 1:357632/1‑150 (MQ=255) tAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATCACCTGGTTTATTGACCGGCGTCTGAACGGCaa > 1:316605/1‑150 (MQ=255) acaacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaaaa > 2:1201602/1‑150 (MQ=255) ccTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaa > 2:296628/1‑150 (MQ=255) aggCCCGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCGTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAg > 1:522184/2‑150 (MQ=255) ggCCCGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGCACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGc > 2:67779/1‑150 (MQ=255) gCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCa > 1:556415/1‑150 (MQ=255) cccGGTA‑ACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCAt > 1:1001118/1‑150 (MQ=255) cGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATgg < 2:815952/150‑1 (MQ=255) | AAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGG > NC_000913/1868717‑1869003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |