Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,456,866:1	+T	7.4%	intergenic (‑54/+149)	sixA ← / ← fadJ	phosphohistidine phosphatase/3‑hydroxyacyl‑CoA dehydrogenase FadJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,456,866	1	.	T	7.4%	265.9 / 16.5	68	intergenic (‑54/+149)	sixA/fadJ	phosphohistidine phosphatase/3‑hydroxyacyl‑CoA dehydrogenase FadJ
Reads supporting (aligned to +/- strand):  ref base . (32/31);  new base T (2/3);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

GCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTC  >  NC_000913/2456717‑2457014                                                                                                                                                       |                                                                                                                                                     gCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCaga                                                                                                                                                       <  1:527288/150‑1 (MQ=255)  cGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCaga‑g                                                                                                                                                     <  2:173953/150‑1 (MQ=255)  cGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCaga‑g                                                                                                                                                     <  2:546058/150‑1 (MQ=255)      aTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGa‑gaaag                                                                                                                                                 <  1:222674/150‑1 (MQ=255)       ttCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGa‑gaaaga                                                                                                                                                <  1:1145448/150‑1 (MQ=255)           tCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAAc                                                                                                                                            >  2:1029813/1‑150 (MQ=255)              caACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTC‑TCTGGTTTCAGATGAAAGAAAACggg                                                                                                                                         <  2:1416925/150‑2 (MQ=38)              caACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTC‑TCTGGTTTCAGATGAAAGAAAACggg                                                                                                                                         <  1:864707/150‑2 (MQ=38)                aCCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTgg                                                                                                                                       <  2:188356/150‑1 (MQ=255)                 ccATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGc                                                                                                                                      >  1:559876/1‑150 (MQ=255)                   aTTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCg                                                                                                                                    >  1:782156/1‑150 (MQ=255)                      aGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTC‑TCTGGTTTCAGATGAAAGAAAACggg                                                                                                                                         <  1:1392481/142‑2 (MQ=38)                      aGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTC‑TCTGGTTTCAGATGAAAGAAAACggg                                                                                                                                         >  2:1392481/1‑141 (MQ=38)                         ggTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAAATTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCAttt                                                                                                                              >  2:95860/1‑149 (MQ=255)                           tCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATtgtg                                                                                                                            <  2:1268193/150‑1 (MQ=255)                            cAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGc                                                                                                                           >  2:344874/1‑150 (MQ=255)                              gagGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCt                                                                                                                         <  1:1365126/150‑1 (MQ=255)                              gagGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCt                                                                                                                         >  2:159612/1‑150 (MQ=255)                               agGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCtt                                                                                                                        >  1:805595/1‑150 (MQ=255)                                ggACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTa                                                                                                                       >  1:164939/1‑150 (MQ=255)                                  aCGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATc                                                                                                                     >  2:1246458/1‑150 (MQ=255)                                  aCGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATc                                                                                                                     <  1:1398491/150‑1 (MQ=255)                                   cGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATcc                                                                                                                    <  1:687248/150‑1 (MQ=255)                                   cGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATcc                                                                                                                    >  1:804190/1‑150 (MQ=255)                                     aaCGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAt                                                                                                                  <  2:316140/150‑1 (MQ=255)                                        ggAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCa                                                                                                               <  2:658467/150‑1 (MQ=255)                                        ggAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCa                                                                                                               <  1:1151316/150‑1 (MQ=255)                                           aTCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCg                                                                                                            <  1:428772/150‑1 (MQ=255)                                            tCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGa                                                                                                           >  2:23556/1‑150 (MQ=255)                                              aCTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAt                                                                                                         <  1:778762/150‑1 (MQ=255)                                               cTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATg                                                                                                        <  1:1317199/150‑1 (MQ=255)                                                 ggcggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGaa                                                                                                      >  2:393618/1‑150 (MQ=255)                                                  gcggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGaaa                                                                                                     <  1:446996/150‑1 (MQ=255)                                                   cggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAAc                                                                                                    >  1:1286998/1‑150 (MQ=255)                                                          gAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGttt                                                                                             >  2:1127226/1‑150 (MQ=255)                                                           aGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGtttt                                                                                            <  2:911478/150‑1 (MQ=255)                                                            gggCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGttttt                                                                                           >  1:1064783/1‑150 (MQ=255)                                                              gCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTa                                                                                         <  1:1029813/150‑1 (MQ=255)                                                                tGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAcc                                                                                       <  1:393618/150‑1 (MQ=255)                                                                  cGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTc                                                                                     <  2:1210610/150‑1 (MQ=255)                                                                      gCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATg                                                                                 >  2:163427/1‑150 (MQ=255)                                                                      gCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATg                                                                                 >  2:848547/1‑150 (MQ=255)                                                                       ccGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATgg                                                                                <  1:621408/150‑1 (MQ=255)                                                                          tGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTCATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGt                                                                             >  2:1065976/1‑150 (MQ=255)                                                                              gCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGt                                                                         >  1:970022/1‑150 (MQ=255)                                                                               cATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATg                                                                                                        >  2:359459/1‑118 (MQ=255)                                                                               cATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATg                                                                                                        <  1:359459/118‑1 (MQ=255)                                                                                    tAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTc                                                                   <  2:340302/150‑1 (MQ=255)                                                                                     aaaaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCa                                                                  >  2:340597/1‑150 (MQ=255)                                                                                      aaaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCaa                                                                 <  1:1127226/150‑1 (MQ=255)                                                                                          cTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                                             >  1:1316691/1‑150 (MQ=255)                                                                                          cTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                                             <  2:782156/150‑1 (MQ=255)                                                                                            tGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCg                                                           >  2:221856/1‑150 (MQ=255)                                                                                               atatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGttt                                                        <  2:119895/150‑1 (MQ=255)                                                                                                     cACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACATCAATGGCGTAAGTATAGTCAATATGCGTTTACAttt                                                  >  2:716699/1‑150 (MQ=255)                                                                                                      aCCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt                                                 <  2:164939/150‑1 (MQ=255)                                                                                                      aCCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt                                                 <  1:944690/150‑1 (MQ=255)                                                                                                       ccGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAttttt                                                >  1:949769/1‑149 (MQ=255)                                                                                                       ccGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTg                                                <  1:734811/150‑1 (MQ=255)                                                                                                       ccGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTg                                                <  2:1064783/150‑1 (MQ=255)                                                                                                        cGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGa                                               <  2:194521/150‑1 (MQ=255)                                                                                                         gCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGACACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATAGGCGTTTACATTTTGAc                                              >  2:636169/1‑150 (MQ=255)                                                                                                         gCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAc                                              >  2:136687/1‑150 (MQ=255)                                                                                                                      aGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGGGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCa                                 >  2:952799/1‑150 (MQ=255)                                                                                                                        aTTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCAtt                               >  1:108560/1‑150 (MQ=255)                                                                                                                            caccGTTTTCTTTC‑TCTGGTTTCAGATGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgc                           >  1:219918/3‑150 (MQ=38)                                                                                                                              ccGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgcgc                         >  2:1005613/1‑150 (MQ=255)                                                                                                                              ccGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgcgc                         <  2:294511/150‑1 (MQ=255)                                                                                                                              ccGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgcgc                         >  1:64007/1‑150 (MQ=255)                                                                                                                                         cATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAtata              <  1:340597/150‑1 (MQ=255)                                                                                                                                          aTCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGGTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAg             >  2:29443/1‑150 (MQ=255)                                                                                                                                                 aCCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGAc      <  2:970022/150‑1 (MQ=255)                                                                                                                                                   cAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACct    <  1:159612/150‑1 (MQ=255)                                                                                                                                                    aga‑gAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACctt   >  2:1006505/1‑150 (MQ=255)                                                                                                                                                     ta‑gaaagaaAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttc  <  1:626128/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                     GCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTC  >  NC_000913/2456717‑2457014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑