Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,107,879 G→T 6.3% A426D (GCT→GAT) ‡ speC ← ornithine decarboxylase, biosynthetic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,8790GT 6.3% 207.5 / 3.8 63A426D (GCT→GAT) ‡speCornithine decarboxylase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base G (26/33);  new base T (2/2);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

TCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTAT  >  NC_000913/3107735‑3108023
                                                                                                                                                |                                                                                                                                                
tCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTg                                                                                                                                             <  1:996535/150‑1 (MQ=255)
   aaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                                          <  1:99065/150‑1 (MQ=255)
   aaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGAGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCACCAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgagc                                                                                                                                          >  1:34834/1‑150 (MQ=255)
     gCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTgcgcgc                                                                                                                                        <  1:860905/150‑1 (MQ=255)
       cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTGCGCGCtg                                                                                                                                      <  1:1286591/150‑2 (MQ=255)
            cACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATc                                                                                                                                 >  2:686988/1‑150 (MQ=255)
             aCTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGAAAGAATAGCCTTGCGCGCTTCAATcc                                                                                                                                >  1:750713/1‑150 (MQ=255)
              cTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATccc                                                                                                                               <  2:84651/150‑1 (MQ=255)
                tCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTa                                                                                                                             <  2:215242/150‑1 (MQ=255)
                       cGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAac                                                                                                                      <  1:980477/150‑1 (MQ=255)
                        ggCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAaca                                                                                                                     >  2:1051313/1‑150 (MQ=255)
                        ggCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAaca                                                                                                                     <  1:1070390/150‑1 (MQ=255)
                         gCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGTGCGGAACAGCTTACAGCTCGCAAGAATAGCCTTGCGCTCTTCAATCCCTATCTCAacac                                                                                                                    <  2:531836/150‑1 (MQ=255)
                         gCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAacac                                                                                                                    <  1:331762/150‑1 (MQ=255)
                         gCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAACTCAacac                                                                                                                    <  2:375756/150‑1 (MQ=255)
                           tCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAacccac                                                                                                                  >  2:562588/1‑150 (MQ=255)
                             aaaaCTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTc                                                                                                                >  1:1394269/1‑150 (MQ=255)
                               aaCTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAg                                                                                                              >  1:78034/1‑150 (MQ=255)
                                 cTGAAAAAACGGTGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGcc                                                                                                            >  1:759205/1‑150 (MQ=255)
                                 cTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGcc                                                                                                            <  2:288033/150‑1 (MQ=255)
                                  tGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGccc                                                                                                           <  2:836425/150‑1 (MQ=255)
                                      aaaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTc                                                                                                                                     <  2:434802/120‑1 (MQ=255)
                                      aaaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTc                                                                                                                                     >  1:434802/1‑120 (MQ=255)
                                                   cTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCActc                                                                                          >  1:652582/1‑150 (MQ=255)
                                                        tAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcccc                                                                                     >  2:249684/1‑150 (MQ=255)
                                                           cacTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTc                                                                                  <  1:911571/150‑1 (MQ=255)
                                                           cacTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTc                                                                                  >  2:907548/1‑150 (MQ=255)
                                                                  gTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAAtt                                                                           >  1:1233994/1‑150 (MQ=255)
                                                                       aTAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGc                                                                      <  1:426562/150‑1 (MQ=255)
                                                                        tAATCGTGCCACAATTTGCCATCAACAACGGGCGTGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATTGGCTTGCGCGCTTCTTTCGCTATATCAACACACTCAGCCCACAGCCGTCGACCACTCTCCACTTGATGAATTTTGTCg                                                                     <  2:34834/150‑1 (MQ=255)
                                                                        tAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCg                                                                     >  1:539455/1‑150 (MQ=255)
                                                                            ccTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTaa                                                                 >  2:1003855/1‑150 (MQ=255)
                                                                              tGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACa                                                               >  2:188986/1‑150 (MQ=255)
                                                                               gCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACAt                                                              >  2:149117/1‑150 (MQ=255)
                                                                                       ttGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgca                                                      <  1:1051313/150‑1 (MQ=255)
                                                                                       ttGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgca                                                      >  1:509563/1‑150 (MQ=255)
                                                                                        tGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcag                                                     <  2:1031828/150‑1 (MQ=255)
                                                                                        tGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcag                                                     <  2:1056946/150‑1 (MQ=255)
                                                                                         gCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcagc                                                    <  1:907548/150‑1 (MQ=255)
                                                                                          ccATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcagca                                                   <  1:1302622/150‑1 (MQ=255)
                                                                                               aatcactggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAg                                              <  2:967510/143‑1 (MQ=255)
                                                                                                   acGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGa                                          <  1:381768/150‑1 (MQ=255)
                                                                                                     gggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATa                                        >  1:1015864/1‑150 (MQ=255)
                                                                                                          ggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAg                                   >  1:1359705/1‑150 (MQ=255)
                                                                                                             tAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGc                                <  1:149117/150‑1 (MQ=255)
                                                                                                                aCGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTgg                             <  1:986651/150‑1 (MQ=255)
                                                                                                                 cGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGt                            <  2:759205/150‑1 (MQ=255)
                                                                                                                   ggCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAg                          <  1:249684/150‑1 (MQ=255)
                                                                                                                    gCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGa                         <  2:539455/150‑1 (MQ=255)
                                                                                                                      ggAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAg                       <  1:686988/150‑1 (MQ=255)
                                                                                                                        aaCAGCTTACAGCGCGCAAGAATAGCCTTGCGCGTTTCAATCCCTATCTCAACACACTCAGCCCACAGCTGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCa                     <  1:505563/150‑1 (MQ=255)
                                                                                                                            gCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACCGCGGATAGAAAGGGCTGGTAGAAGCATGGa                 >  1:672459/1‑150 (MQ=255)
                                                                                                                             cTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAg                <  2:324820/150‑1 (MQ=255)
                                                                                                                              ttACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGc               <  1:365830/150‑1 (MQ=255)
                                                                                                                                 aaGCGCGCAAGGCTATTCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATa            >  2:651030/2‑150 (MQ=255)
                                                                                                                                  aGCGCGCAAGGCTATTCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATaa           >  2:658765/1‑150 (MQ=255)
                                                                                                                                   gcgcgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCa                     >  2:812821/1‑139 (MQ=255)
                                                                                                                                   gcgcgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCa                     <  1:812821/139‑1 (MQ=255)
                                                                                                                                    cgcgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAg         >  1:1252371/1‑150 (MQ=255)
                                                                                                                                      cgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGc       >  2:192886/1‑150 (MQ=255)
                                                                                                                                           gAATAGCCTTGCGCGCTTCAATCCCTATCTCAAGACACTGTGCCCACAGGCGAGGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATGGAGTGCAGCATACAGCGGATAGAAAGGGCTGGTAGATGCTTGGAGCATAAAGGCGTTAt  <  2:513419/150‑1 (MQ=255)
                                                                                                                                           gAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTAt  >  2:665900/1‑150 (MQ=255)
                                                                                                                                           gAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTAt  >  2:225687/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTAT  >  NC_000913/3107735‑3108023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑