Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,323,445 T→C 5.9% N209S (AAC→AGC)  glmM ← phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,323,4450TC 5.9% 205.4 / 2.4 68N209S (AAC→AGC) glmMphosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base T (28/36);  new base C (2/2);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

ACTTTATTGCCTTCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTC  >  NC_000913/3323301‑3323593
                                                                                                                                                |                                                                                                                                                    
aCTTTATTGCCTTCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGc                                                                                                                                                 <  2:101921/150‑1 (MQ=255)
aCTTTATTGCCTTCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGc                                                                                                                                                 >  1:1400145/1‑150 (MQ=255)
    tATTGCCTTCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTcac                                                                                                                                             >  2:390891/1‑150 (MQ=255)
     aTTGCCTTCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTcaca                                                                                                                                            >  2:273887/1‑150 (MQ=255)
        gCCTTCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAAcc                                                                                                                                         <  2:1245436/150‑1 (MQ=255)
        gCCTTCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAAcc                                                                                                                                         <  2:206733/150‑1 (MQ=255)
         ccTTCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCg                                                                                                                                        <  1:232922/150‑1 (MQ=255)
          cTTCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGa                                                                                                                                       >  2:904276/1‑150 (MQ=255)
          cTTCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGa                                                                                                                                       >  1:169888/1‑150 (MQ=255)
           ttCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGAt                                                                                                                                      <  2:1075081/150‑1 (MQ=255)
            tCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATa                                                                                                                                     <  1:1144059/150‑1 (MQ=255)
              aTGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGc                                                                                                                                   <  2:1305159/150‑1 (MQ=255)
               tGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGCTTGGCTCACAACCGATAGCg                                                                                                                                  >  2:1000196/1‑150 (MQ=255)
               tGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGCTTGGCTCACAACCGATAGCg                                                                                                                                  >  2:995250/1‑150 (MQ=255)
                     aCCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAAcc                                                                                                                                         <  2:1176467/137‑1 (MQ=255)
                     aCCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAAcc                                                                                                                                         >  1:1176467/1‑137 (MQ=255)
                     aCCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACg                                                                                                                            <  2:1117908/150‑1 (MQ=255)
                      ccATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGt                                                                                                                           >  2:1102256/1‑150 (MQ=255)
                       cATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTCGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGtt                                                                                                                          >  1:723028/1‑150 (MQ=255)
                            tCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGccc                                                                                                                     <  1:662853/150‑1 (MQ=255)
                                 cgATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGt                                                                                                                >  1:1009498/1‑150 (MQ=255)
                                  gATCGCCATCGCCGTCGAAGGCAATACCGATATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCTTTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTGGCCCCCAGtt                                                                                                               <  2:21226/150‑1 (MQ=255)
                                       ccatcgccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCgcgc                                                                                                          <  2:1400145/150‑1 (MQ=255)
                                       ccatcgccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCgcgc                                                                                                          >  2:118417/1‑150 (MQ=255)
                                       ccatcgccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCgcgc                                                                                                          <  2:169888/150‑1 (MQ=255)
                                          tcgccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCgcagc                                                                                                       >  1:118621/1‑150 (MQ=255)
                                          tcgccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCgcagc                                                                                                       >  1:123619/1‑150 (MQ=255)
                                            gccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCAc                                                                                                     >  2:1406634/1‑150 (MQ=255)
                                            gccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCAc                                                                                                     >  2:193594/1‑150 (MQ=255)
                                                tCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTc                                                                                                 <  1:860302/150‑1 (MQ=255)
                                                 cGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCg                                                                                                >  2:565272/1‑150 (MQ=255)
                                                  gAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCgg                                                                                               >  1:1007069/1‑150 (MQ=255)
                                                         aTACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCCGAACGTGCTGCGCGaac                                                                                        <  1:185110/150‑3 (MQ=255)
                                                         aTACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCCGAACGTGCTGCGCGaac                                                                                        <  1:1370053/150‑3 (MQ=255)
                                                           aCCGAGATCCGCTTTTTCCGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGAtgtg                                                                                      <  1:1002448/150‑1 (MQ=255)
                                                            ccGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCCGAACGTGCTGCGCGaactgg                                                                                     <  1:197759/150‑6 (MQ=255)
                                                             cGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGAt                                                                                    <  2:235152/150‑1 (MQ=255)
                                                                gaTCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAg                                                                                 <  1:904276/150‑1 (MQ=255)
                                                                gaTCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAg                                                                                 >  2:662842/1‑150 (MQ=255)
                                                                 aTCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGt                                                                                >  1:1410053/1‑150 (MQ=255)
                                                                  tCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTc                                                                               >  1:768470/1‑150 (MQ=255)
                                                                           tCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGttt                                                                      <  2:1007069/150‑1 (MQ=255)
                                                                               ccagcACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGcac                                                                  <  1:5726/150‑1 (MQ=255)
                                                                                   cacaCGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATc                                                              <  1:273887/150‑1 (MQ=255)
                                                                                    acacGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGCTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATcc                                                             <  1:1000196/150‑1 (MQ=255)
                                                                                    acacGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGCTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATcc                                                             <  1:995250/150‑1 (MQ=255)
                                                                                     cacGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGc                                                                    <  2:1295176/142‑1 (MQ=255)
                                                                                     cacGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGc                                                                    >  1:1295176/1‑142 (MQ=255)
                                                                                       cGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATccacc                                                          >  2:654001/1‑150 (MQ=255)
                                                                                       cGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATccacc                                                          <  1:390891/150‑1 (MQ=255)
                                                                                        gAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGAtg                                                                                        >  1:460262/1‑119 (MQ=255)
                                                                                        gAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGAtg                                                                                        <  2:460262/119‑1 (MQ=255)
                                                                                            cTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAAt                                                     >  1:499637/1‑150 (MQ=255)
                                                                                              ggtgcgcgcGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCt                                                   <  1:1213006/147‑1 (MQ=255)
                                                                                              ggAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCt                                                   <  2:1046992/150‑1 (MQ=255)
                                                                                                aGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTc                                                 <  2:1390705/150‑1 (MQ=255)
                                                                                                       gAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCAc                                          <  1:193594/150‑1 (MQ=255)
                                                                                                             cGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGc                                    <  2:1291554/150‑1 (MQ=255)
                                                                                                                   cccccACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAgtt                              >  1:1163895/1‑150 (MQ=255)
                                                                                                                    ccccACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAgttc                             >  1:1428833/1‑150 (MQ=255)
                                                                                                                      ccACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACGATCCACCACAATCTTCAGTTCACTGAGGCTAAgttcgt                           >  1:865672/1‑150 (MQ=255)
                                                                                                                        tcTTCGGCATTGATGTTTACACCGTTTGGGCGAGAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCAGCGTTTGCACAATGCTGCACAATCTTCAGTTCTCTGAGGCTAAgttcgttc                         <  2:304258/149‑1 (MQ=255)
                                                                                                                        aCTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCTCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAgttcgttc                         <  1:118417/150‑1 (MQ=255)
                                                                                                                           tCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGAGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCggg                      <  1:763244/150‑1 (MQ=255)
                                                                                                                               cATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACg                  <  2:1009498/150‑1 (MQ=255)
                                                                                                                                 ttGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTg                <  1:1102256/150‑1 (MQ=255)
                                                                                                                                         tACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCa        >  2:1299317/1‑150 (MQ=255)
                                                                                                                                               gTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTc  >  2:1281619/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
ACTTTATTGCCTTCATGGTCAACCATAATCACGCGATCGCCATCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTC  >  NC_000913/3323301‑3323593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑