Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,323,490	A→C	8.1%	L194R (CTG→CGG)	glmM ←	phosphoglucosamine mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,323,490	0	A	C	8.1%	190.0 / 8.6	62	L194R (CTG→CGG)	glmM	phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base A (29/28);  new base C (2/3);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

TCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTAC  >  NC_000913/3323343‑3323634                                                                                                                                                    |                                                                                                                                                 tcgccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCgcagc                                                                                                                                                >  1:118621/1‑150 (MQ=255) tcgccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCgcagc                                                                                                                                                >  1:123619/1‑150 (MQ=255)   gccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCAc                                                                                                                                              >  2:1406634/1‑150 (MQ=255)   gccGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCAc                                                                                                                                              >  2:193594/1‑150 (MQ=255)       tCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTc                                                                                                                                          <  1:860302/150‑1 (MQ=255)        cGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCg                                                                                                                                         >  2:565272/1‑150 (MQ=255)         gAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCgg                                                                                                                                        >  1:1007069/1‑150 (MQ=255)                aTACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCCGAACGTGCTGCGCGaac                                                                                                                                 <  1:185110/150‑3 (MQ=255)                aTACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCCGAACGTGCTGCGCGaac                                                                                                                                 <  1:1370053/150‑3 (MQ=255)                  aCCGAGATCCGCTTTTTCCGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGAtgtg                                                                                                                               <  1:1002448/150‑1 (MQ=255)                   ccGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCCGAACGTGCTGCGCGaactgg                                                                                                                              <  1:197759/150‑6 (MQ=255)                    cGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGAt                                                                                                                             <  2:235152/150‑1 (MQ=255)                       gaTCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAg                                                                                                                          <  1:904276/150‑1 (MQ=255)                       gaTCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAg                                                                                                                          >  2:662842/1‑150 (MQ=255)                        aTCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGt                                                                                                                         >  1:1410053/1‑150 (MQ=255)                         tCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTc                                                                                                                        >  1:768470/1‑150 (MQ=255)                                  tCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGttt                                                                                                               <  2:1007069/150‑1 (MQ=255)                                      ccagcACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGcac                                                                                                           <  1:5726/150‑1 (MQ=255)                                          cacaCGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATc                                                                                                       <  1:273887/150‑1 (MQ=255)                                           acacGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGCTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATcc                                                                                                      <  1:995250/150‑1 (MQ=255)                                           acacGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGCTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATcc                                                                                                      <  1:1000196/150‑1 (MQ=255)                                            cacGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGc                                                                                                             >  1:1295176/1‑142 (MQ=255)                                            cacGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGc                                                                                                             <  2:1295176/142‑1 (MQ=255)                                              cGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATccacc                                                                                                   >  2:654001/1‑150 (MQ=255)                                              cGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATccacc                                                                                                   <  1:390891/150‑1 (MQ=255)                                               gAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGAtg                                                                                                                                 >  1:460262/1‑119 (MQ=255)                                               gAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGAtg                                                                                                                                 <  2:460262/119‑1 (MQ=255)                                                   cTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAAt                                                                                              >  1:499637/1‑150 (MQ=255)                                                     ggtgcgcgcGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCt                                                                                            <  1:1213006/147‑1 (MQ=255)                                                     ggAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCt                                                                                            <  2:1046992/150‑1 (MQ=255)                                                       aGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTc                                                                                          <  2:1390705/150‑1 (MQ=255)                                                              gAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCAc                                                                                   <  1:193594/150‑1 (MQ=255)                                                                    cGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGc                                                                             <  2:1291554/150‑1 (MQ=255)                                                                          cccccACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAgtt                                                                       >  1:1163895/1‑150 (MQ=255)                                                                           ccccACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAgttc                                                                      >  1:1428833/1‑150 (MQ=255)                                                                             ccACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACGATCCACCACAATCTTCAGTTCACTGAGGCTAAgttcgt                                                                    >  1:865672/1‑150 (MQ=255)                                                                               tcTTCGGCATTGATGTTTACACCGTTTGGGCGAGAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCAGCGTTTGCACAATGCTGCACAATCTTCAGTTCTCTGAGGCTAAgttcgttc                                                                  <  2:304258/149‑1 (MQ=255)                                                                               aCTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCTCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAgttcgttc                                                                  <  1:118417/150‑1 (MQ=255)                                                                                  tCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGAGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCggg                                                               <  1:763244/150‑1 (MQ=255)                                                                                      cATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACg                                                           <  2:1009498/150‑1 (MQ=255)                                                                                        ttGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTg                                                         <  1:1102256/150‑1 (MQ=255)                                                                                                tACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCa                                                 >  2:1299317/1‑150 (MQ=255)                                                                                                      gTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTc                                           >  2:1281619/1‑150 (MQ=255)                                                                                                         tGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGat                                        <  2:723028/150‑1 (MQ=255)                                                                                                           gCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGatat                                      >  1:624441/1‑150 (MQ=255)                                                                                                            cTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGAtata                                     >  1:1079387/1‑150 (MQ=255)                                                                                                             tCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACGATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAg                                    <  2:865672/150‑1 (MQ=255)                                                                                                             tCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAg                                    >  1:154096/1‑150 (MQ=255)                                                                                                              cacaACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGc                                   >  2:912432/1‑150 (MQ=255)                                                                                                              cacaACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGc                                   >  1:430160/1‑150 (MQ=255)                                                                                                                      aTAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCcgcg                           <  2:1024287/150‑1 (MQ=255)                                                                                                                         gCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCa                        >  1:28930/1‑150 (MQ=255)                                                                                                                         gCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCa                        >  1:1374109/1‑150 (MQ=255)                                                                                                                         gCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCa                        <  1:1281619/150‑1 (MQ=255)                                                                                                                              aaCGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAAc                   >  2:544160/1‑150 (MQ=255)                                                                                                                                     gCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACgg            >  2:348916/1‑150 (MQ=255)                                                                                                                                      cccccAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggc           >  1:879018/1‑150 (MQ=255)                                                                                                                                      cccccAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggc           <  1:662842/150‑1 (MQ=255)                                                                                                                                        cccAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggctg         >  2:857979/1‑150 (MQ=255)                                                                                                                                          cAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggctggc       <  1:565272/150‑1 (MQ=255)                                                                                                                                           acatCGCGCCGAACGTGCTGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACggctggct      >  1:510716/4‑150 (MQ=255)                                                                                                                                            catCGCGCCGAACGTGCTGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCtt     >  1:1127329/3‑150 (MQ=255)                                                                                                                                               cgcgCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTAc  >  1:628334/1‑150 (MQ=255)                                                                                                                                               cgcgCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTAc  >  2:672195/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                 TCGCCGTCGAAGGCAATACCGAGATCCGCTTTTTCAGCCAGCACACGAGCCTGGAGCGCGCGAACGTCGGTAGCCCCCACTTCGGCATTGATGTTTACACCGTTTGGCTCACAACCGATAGCGATAACGTTCGCCCCCAGTTCGCGCAGCACGTTCGGCGCGATGTGATAAGTCGCACCGTTTGCACAATCCACCACAATCTTCAGTTCACTGAGGCTAAGTTCGTTCGGGAACGTGGCTTTGCAAAACTCGATATAGCGACCCGCGGCATCAACGATACGGCTGGCTTTAC  >  NC_000913/3323343‑3323634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑