Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,491,705 A→C 7.5% intergenic (‑85/+20) yhfG ← / ← ppiA DUF2559 domain‑containing protein YhfG/peptidyl‑prolyl cis‑trans isomerase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,491,7050AC 7.5% 142.3 / 3.4 52intergenic (‑85/+20)yhfG/ppiADUF2559 domain‑containing protein YhfG/peptidyl‑prolyl cis‑trans isomerase A
Reads supporting (aligned to +/- strand):  ref base A (20/28);  new base C (2/2);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

ATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAA  >  NC_000913/3491561‑3491852
                                                                                                                                                 |                                                                                                                                                   
attacgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGcc                                                                                                                                                <  2:1057601/150‑1 (MQ=255)
    cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcgcg                                                                                                                                            >  2:1022566/1‑150 (MQ=255)
      attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTGTCGCCGGGAGAGCAGAAGCGCGGGCCAGATTGCCcgcgcg                                                                                                                                          >  2:254024/1‑150 (MQ=255)
          cgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGaa                                                                                                                                      <  2:1312970/150‑1 (MQ=255)
                 gCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTa                                                                                                                               <  1:1102845/150‑1 (MQ=255)
                    cccAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGAAGATTTGCCCCGCGAGGAATAAATACgg                                                                                                                            >  1:148265/1‑150 (MQ=255)
                          gaCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGAc                                                                                                                      <  1:1022566/150‑1 (MQ=255)
                            cGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAATATTGCCCGCGCGAGAAATCAATACGGCAGGACtt                                                                                                                    >  1:864532/1‑150 (MQ=255)
                                 cTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTACATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGCAGCGCGGGCAAGAATGCCCGCGCGAGAAATGATTACGGCAGGACTGTATCg                                                                                                               >  2:1320864/1‑150 (MQ=255)
                                  ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGAC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGcag                                                                                                              <  1:959378/150‑3 (MQ=255)
                                  ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGAC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGcag                                                                                                              <  1:964255/150‑3 (MQ=255)
                                   tttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTGGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGCTTGCCCGCGCGGGAAAACATTTCGGCAGGCCTTTTGCggc                                                                                                             >  1:1239755/1‑149 (MQ=255)
                                      gCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAAATTAGCCCCCCCAGAAATAATTAAAGCAGCACGATATCGGaaaa                                                                                                          >  2:661581/1‑149 (MQ=255)
                                       cTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTTCGGCAGGACTTTAGCGGAAAgg                                                                                                         <  2:1102833/150‑1 (MQ=255)
                                        ttATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGa                                                                                                        >  1:860734/1‑150 (MQ=255)
                                         tATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGa                                                                                                        >  2:1165892/1‑150 (MQ=255)
                                         tATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCTGAAAGGAt                                                                                                       <  1:1360323/150‑1 (MQ=255)
                                          aTCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATa                                                                                                      <  1:508338/150‑1 (MQ=255)
                                                  gTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCgg                                                                                              <  1:6885/150‑1 (MQ=255)
                                                   tttCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGt                                                                                             <  2:9525/150‑1 (MQ=255)
                                                      cttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAAAATTCCCCCGCGAGAAAAAAATAAGGCACGCAATTAGCGGAAAAGAAAACAAACGGtttt                                                                                          >  2:312805/1‑150 (MQ=255)
                                                       ttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGCCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTg                                                                                         <  2:1298127/150‑1 (MQ=255)
                                                        tcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGa                                                                                        >  1:810815/1‑150 (MQ=255)
                                                         cACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGAc                                                                                       <  2:1350168/150‑1 (MQ=255)
                                                           cTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACgg                                                                                     <  2:972934/150‑1 (MQ=255)
                                                                            cGTGTCCATTTGCCACAAGTCTAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGAc                                                                    <  1:980972/150‑1 (MQ=255)
                                                                              tgtCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGAcct                                                                  <  2:465394/150‑2 (MQ=255)
                                                                              tgtCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAAACAATACGGCAGGACTTTAGCGGAAAAGATAACAACCGGTTTTGAAGGCACAATCTGGTAACGACCa                                                                  >  2:673138/1‑150 (MQ=255)
                                                                                 ccATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAGATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACg                                                               >  1:992916/1‑150 (MQ=255)
                                                                                   aTTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTc                                                             <  1:242652/150‑1 (MQ=255)
                                                                                        ccACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAg                                                        <  1:1044906/150‑1 (MQ=255)
                                                                                                    gCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGG‑TTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGa                                           >  2:1255911/1‑150 (MQ=255)
                                                                                                           aaTCCGGGTTACGCCGGGAGAGCAGAAGCGCTGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCt                                      <  1:323446/150‑1 (MQ=255)
                                                                                                             tCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAAt                                        >  1:564376/1‑146 (MQ=255)
                                                                                                             tCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAAt                                        <  2:564376/146‑1 (MQ=255)
                                                                                                             tCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTa                                    <  1:661581/150‑1 (MQ=255)
                                                                                                                gggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCg                                 <  1:799614/150‑1 (MQ=255)
                                                                                                                   ttACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGG‑TTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCaa                             <  1:1255911/150‑1 (MQ=255)
                                                                                                                    tACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGTACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCaa                             <  2:148265/150‑1 (MQ=255)
                                                                                                                       gCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGt                          <  1:312805/150‑1 (MQ=255)
                                                                                                                            gagaGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATg                     <  2:1239755/150‑1 (MQ=255)
                                                                                                                                   gAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACg                                                                       >  1:926746/1‑93 (MQ=255)
                                                                                                                                   gAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACg                                                                       <  2:926746/93‑1 (MQ=255)
                                                                                                                                     cgcgcGGGCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc               <  2:1322520/146‑1 (MQ=255)
                                                                                                                                     cgcgcGGGCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc               >  1:1322520/2‑147 (MQ=255)
                                                                                                                                     aGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGc                                                                                    >  1:728850/1‑78 (MQ=255)
                                                                                                                                     aGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGc                                                                                    <  2:728850/78‑1 (MQ=255)
                                                                                                                                     aGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTcacc            >  1:1410576/1‑150 (MQ=255)
                                                                                                                                     aGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTcacc            >  1:1230776/1‑150 (MQ=255)
                                                                                                                                          gggCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       <  2:279243/150‑1 (MQ=255)
                                                                                                                                          gggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACttt       >  1:834349/1‑150 (MQ=255)
                                                                                                                                               aGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCaa  >  1:845731/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGC‑AAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAA  >  NC_000913/3491561‑3491852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑