Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,561,296	C→G	6.0%	A53P (GCG→CCG)	glpG ←	rhomboid protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,561,296	0	C	G	6.0%	221.6 / 4.6	67	A53P (GCG→CCG)	glpG	rhomboid protease
Reads supporting (aligned to +/- strand):  ref base C (28/35);  new base G (2/2);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

TCACCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTA  >  NC_000913/3561155‑3561441                                                                                                                                              |                                                                                                                                                  tcaCCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCg                                                                                                                                           <  2:669489/150‑1 (MQ=255)  caCCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGc                                                                                                                                          >  2:524575/1‑150 (MQ=255)  caCCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGc                                                                                                                                          <  2:729829/150‑1 (MQ=255)  caCCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGc                                                                                                                                          >  2:845993/1‑150 (MQ=255)  caCCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGc                                                                                                                                          <  2:96225/150‑1 (MQ=255)  caCCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGc                                                                                                                                          >  1:148674/1‑150 (MQ=255)   aCCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGcc                                                                                                                                         <  2:1273278/150‑1 (MQ=255)   aCCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGcc                                                                                                                                         <  1:643986/150‑1 (MQ=255)   aCCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGcc                                                                                                                                         <  1:1245256/150‑1 (MQ=255)    cccAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGccc                                                                                                                                        >  1:1116603/1‑150 (MQ=255)      cAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGt                                                                                                                                      >  2:559312/1‑150 (MQ=255)         gTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcg                                                                                                                                   >  1:79811/1‑150 (MQ=255)         gTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcg                                                                                                                                   <  2:663092/150‑1 (MQ=255)          ttACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTAcgc                                                                                                                                  <  2:593397/150‑1 (MQ=255)                  ccTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGcc                                                                                                                          <  1:1245010/150‑1 (MQ=255)                  ccTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGcc                                                                                                                          <  2:288065/150‑1 (MQ=255)                     gcgcGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGAGCTGGCGCGTTTTCTCGAAAACCCGGCAGATCCGcg                                                                                                                                         <  1:1223671/132‑2 (MQ=37)                     gcgcGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGAGCTGGCGCGTTTTCTCGAAAACCCGGCAGATCCGcg                                                                                                                                         >  2:1223671/1‑131 (MQ=37)                      cgcgTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTggg                                                                                                                      >  2:957063/1‑150 (MQ=255)                      cgcgTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTggg                                                                                                                      <  1:524575/150‑1 (MQ=255)                            cACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCAGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCAt                                                                                                                >  2:72267/1‑150 (MQ=255)                               gCAAGGCGGCAAAGAAAGGATAACGTCGATAATGCAGGCCTCTTCAGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGTATCTGCCGGGTTTTCGAGAAAACGCGCGAGCTCCGCCCGTACGCTCTCGGCCTTGGACTCATCCg                                                                                                             <  2:480047/150‑1 (MQ=255)                               gCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCg                                                                                                             <  1:640675/150‑1 (MQ=255)                                        cAAAGAAAGGATAAAGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGa                                                                                                    >  2:1025673/1‑150 (MQ=255)                                         aaagaaagGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGAc                                                                                                   <  1:1005760/150‑1 (MQ=255)                                           agaaagGATAACGGCGATAATGCAGGTCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACAt                                                                                                 >  2:976305/1‑150 (MQ=255)                                           agaaagGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACAt                                                                                                 >  1:10894/1‑150 (MQ=255)                                            gaaagGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATc                                                                                                >  1:450366/1‑150 (MQ=255)                                              aagGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGc                                                                                              <  1:833435/150‑1 (MQ=255)                                                  aTAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTg                                                                                          <  2:1249790/150‑1 (MQ=255)                                                  aTAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTg                                                                                          <  2:1233695/150‑1 (MQ=255)                                                    aaCGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGt                                                                                        >  2:997252/1‑150 (MQ=255)                                                      cGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTa                                                                                      >  1:584084/1‑150 (MQ=255)                                                             caggCAGGCCACTGCCGGTATGGCCTGCCTGACAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATgttgtt                                                                               >  2:939398/4‑150 (MQ=255)                                                              aTGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGttgttg                                                                              <  2:10894/150‑1 (MQ=255)                                                              aTGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGttgttg                                                                              <  1:58890/150‑1 (MQ=255)                                                              aTGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGttgttg                                                                              <  1:248482/150‑1 (MQ=255)                                                               tGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGa                                                                             >  1:803720/1‑150 (MQ=255)                                                                      cACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGa                                                                      <  2:317040/150‑1 (MQ=255)                                                                         tGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCTTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGGTGTTGAATCGTGAGGa                                                                   <  2:478090/150‑1 (MQ=255)                                                                             ggTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAac                                                               >  1:261477/1‑150 (MQ=255)                                                                               tATGGCCTGCCTGCCAGCTCGCCGCCATATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAacag                                                             <  2:639086/150‑2 (MQ=255)                                                                                aTGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAcc                                                            >  1:358825/1‑150 (MQ=255)                                                                                aTGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAcc                                                            >  1:353578/1‑150 (MQ=255)                                                                                 tGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAccc                                                           >  1:788983/1‑150 (MQ=255)                                                                                  ggCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCt                                                          >  1:766684/1‑150 (MQ=255)                                                                                        cctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCACACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCg                                                    <  1:324015/150‑1 (MQ=255)                                                                                           gccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCa                                                 <  2:353578/150‑1 (MQ=255)                                                                                           gccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCa                                                 <  2:358825/150‑1 (MQ=255)                                                                                           gccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCa                                                 <  2:1013715/150‑1 (MQ=255)                                                                                              aGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGt                                              >  2:1138226/1‑150 (MQ=255)                                                                                               gCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTa                                             >  2:1357415/1‑150 (MQ=255)                                                                                                cTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTaa                                            >  2:1405641/1‑150 (MQ=255)                                                                                                cTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTaa                                            <  2:1116603/150‑1 (MQ=255)                                                                                                  cgccgcCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATc                                          <  2:79811/150‑1 (MQ=255)                                                                                                   gccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATca                                         <  1:957063/150‑1 (MQ=255)                                                                                                    ccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACCCCCTGCGTCGCCATGTAATcaa                                        >  1:1418544/1‑150 (MQ=255)                                                                                                    ccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATcaa                                        <  1:397738/150‑1 (MQ=255)                                                                                                               cgcgGAGCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTgc                             <  1:909912/150‑1 (MQ=255)                                                                                                                   gagcTGGCGCGTTTTCTCGAAAACCCGGCAGATCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCa                         >  1:42674/4‑150 (MQ=38)                                                                                                                              ttttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGGCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTa              <  2:134296/150‑1 (MQ=255)                                                                                                                               tttCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAg             <  1:939398/150‑1 (MQ=255)                                                                                                                                ttctcgaAAACCCGGCAGATCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGc            <  2:42674/145‑1 (MQ=255)                                                                                                                                    agaAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCaaaa        >  2:1224569/1‑150 (MQ=255)                                                                                                                                       aaaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAagag     <  1:1224569/150‑1 (MQ=255)                                                                                                                                          cgcgCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTa  >  2:243631/1‑150 (MQ=255)                                                                                                                                          cgcgCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTa  >  1:976456/1‑150 (MQ=255)                                                                                                                                              |                                                                                                                                                  TCACCCAGGTTACCGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAAAAGAGGTA  >  NC_000913/3561155‑3561441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑