Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,588,561 A→C 6.8% V207G (GTG→GGG)  ugpC ← sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,588,5610AC 6.8% 170.4 / 5.3 59V207G (GTG→GGG) ugpCsn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (30/25);  new base C (2/2);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

AGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAAC  >  NC_000913/3588414‑3588710
                                                                                                                                                   |                                                                                                                                                     
aGTTAGAAATACTTTCATTCGTTATTAACGCGGCCTGTCAGCAGGTTAATCGCAGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCAcc                                                                                                                                                     >  2:1036468/1‑150 (MQ=255)
 gTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCa                                                                                                                                                    >  1:81005/1‑150 (MQ=255)
  ttCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTTGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCAt                                                                                                                                                   <  2:892864/150‑1 (MQ=255)
           cGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTg                                                                                                                                          >  1:153089/1‑150 (MQ=255)
              gCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGc                                                                                                                                       >  2:1291483/1‑150 (MQ=255)
                      tATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCa                                                                                                                               <  1:522187/150‑1 (MQ=255)
                        ttCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATc                                                                                                                             <  2:599438/150‑1 (MQ=255)
                          cACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGc                                                                                                                           >  2:1419208/1‑150 (MQ=255)
                               ggCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCaa                                                                                                                      <  1:1036468/150‑1 (MQ=255)
                                         cagGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTg                                                                                                            <  2:30676/150‑1 (MQ=255)
                                           ggTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTTGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAg                                                                                                          <  2:259316/150‑1 (MQ=255)
                                                      ggACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGt                                                                                               <  1:98931/150‑1 (MQ=255)
                                                      ggACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGt                                                                                               <  1:822178/150‑1 (MQ=255)
                                                       gACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTg                                                                                              <  2:752835/150‑1 (MQ=255)
                                                           gCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGt                                                                                          >  2:1180088/1‑150 (MQ=255)
                                                           gCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGt                                                                                          >  2:994672/1‑150 (MQ=255)
                                                           gCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGt                                                                                          >  1:576205/1‑150 (MQ=255)
                                                             cGATAAAACTCGCTAGAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCg                                                                                        <  2:338171/150‑1 (MQ=255)
                                                             cGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCg                                                                                        >  2:1127398/1‑150 (MQ=255)
                                                              gATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAAc                                                                                                                     <  2:272963/120‑1 (MQ=255)
                                                              gATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAAc                                                                                                                     >  1:272963/1‑120 (MQ=255)
                                                                 aaaaCTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGtttt                                                                                    >  2:1047509/1‑150 (MQ=255)
                                                                  aaaCTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACATCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTc                                                                                   >  2:738505/1‑150 (MQ=255)
                                                                    aCTCGCTACAAACAGGCAGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGAAACACCGCCGTTCCTCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGAGAGTAACGTAGACTGAAGACGTTTACac                                                                                 >  2:989131/1‑149 (MQ=255)
                                                                     cTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAgg                                                                                <  2:1321403/150‑1 (MQ=255)
                                                                          tACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTACATCACCATTACTCGCTGGGCGAGCCTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGCCGACg                                                                           >  2:1007186/1‑150 (MQ=255)
                                                                            cAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGt                                                                         <  2:81005/150‑1 (MQ=255)
                                                                            cAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGt                                                                         <  1:738505/150‑1 (MQ=255)
                                                                              aaCAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGc                                                                       >  2:473354/1‑150 (MQ=255)
                                                                                      ggCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGAGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTg                                                               >  2:165975/1‑150 (MQ=255)
                                                                                       gCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGc                                                              <  2:1231687/150‑1 (MQ=255)
                                                                                        cGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCa                                                             >  2:290828/1‑150 (MQ=255)
                                                                                         gggCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAg                                                            <  1:1419208/150‑1 (MQ=255)
                                                                                                tCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGa                                                     >  2:644705/1‑150 (MQ=255)
                                                                                                   tAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCATCTGTTGCAGTTCAAGACGc                                                  >  2:661474/1‑150 (MQ=255)
                                                                                                   tAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGc                                                  >  2:656553/1‑150 (MQ=255)
                                                                                                       cTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCt                                              >  1:611807/1‑150 (MQ=255)
                                                                                                               ggTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACgcgc                                      <  1:290828/150‑1 (MQ=255)
                                                                                                                  gtgCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGc                                   >  1:1310622/1‑150 (MQ=255)
                                                                                                                      cAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGt                                                                         <  1:1321501/108‑1 (MQ=255)
                                                                                                                      cAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGt                                                                         >  2:1321501/1‑108 (MQ=255)
                                                                                                                       aaTCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGc                              <  1:588830/150‑1 (MQ=255)
                                                                                                                           tGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCg                          <  2:153089/150‑1 (MQ=255)
                                                                                                                              tCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAgg                       >  2:713823/1‑150 (MQ=255)
                                                                                                                              tCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGAAGAGAGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAgg                       >  1:1334202/1‑150 (MQ=255)
                                                                                                                                ggCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGtt                     >  2:450662/1‑150 (MQ=255)
                                                                                                                                  cAACACCGCCGTTCATCCCCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGa                         <  1:926194/144‑1 (MQ=255)
                                                                                                                                  cAACACCGCCGTTCATCCCCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGa                         >  2:926194/1‑144 (MQ=255)
                                                                                                                                  cAACACCGCCGTTCATCCCCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGa                         >  2:921431/1‑144 (MQ=255)
                                                                                                                                  cAACACCGCCGTTCATCCCCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGa                         <  1:921431/144‑1 (MQ=255)
                                                                                                                                    acacCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTagag                 <  1:1180088/150‑1 (MQ=255)
                                                                                                                                     cacCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTagaga                >  1:1140016/1‑150 (MQ=255)
                                                                                                                                       ccgccgTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCACAGCTTGGCATCGAGGTTAGAGAGc              >  1:377109/1‑150 (MQ=255)
                                                                                                                                         gccgTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCAcg                                         <  2:26692/121‑1 (MQ=255)
                                                                                                                                         gccgTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCAcg                                         >  1:26692/1‑121 (MQ=255)
                                                                                                                                          ccgTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGc           <  2:430262/150‑1 (MQ=255)
                                                                                                                                          ccgTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGc           >  1:649846/1‑150 (MQ=255)
                                                                                                                                           cgTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCt          <  1:1047509/150‑1 (MQ=255)
                                                                                                                                             ttCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCtca        <  1:1291483/150‑1 (MQ=255)
                                                                                                                                                   aCCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAAc  <  2:611807/150‑1 (MQ=255)
                                                                                                                                                   aCCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAAc  <  1:799789/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGTTCGAAATGCGTGCCTTCGTTATTCACGCGGCCTGTCAGCAGGTTCATCGCCGGACTGCCGATAAAACTCGCTACAAACAGGCTGGCGGGCTTTTCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAAC  >  NC_000913/3588414‑3588710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑