Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,674,395 A→T 5.3% intergenic (+20/‑391) yhjD → / → yhjE putative transporter YhjD/putative transporter YhjE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,674,3950AT 5.3% 310.9 / 5.4 94intergenic (+20/‑391)yhjD/yhjEputative transporter YhjD/putative transporter YhjE
Reads supporting (aligned to +/- strand):  ref base A (39/50);  new base T (3/2);  total (42/52)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ATTTGGTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAAT  >  NC_000913/3674246‑3674544
                                                                                                                                                     |                                                                                                                                                     
aTTTGGTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCATGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTa                                                                                                                                                       >  1:17017/1‑150 (MQ=255)
  ttGGTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTaaa                                                                                                                                                     >  2:682638/1‑150 (MQ=255)
  ttGGTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTaaa                                                                                                                                                     >  1:368548/1‑150 (MQ=255)
     gTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGc                                                                                                                                                  >  2:867170/1‑150 (MQ=255)
     gTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGc                                                                                                                                                  >  2:1226289/1‑150 (MQ=255)
     gTTCCATGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGc                                                                                                                                                  >  1:1375813/1‑150 (MQ=255)
      ttCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAATGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGcc                                                                                                                                                 >  2:306091/1‑150 (MQ=255)
      ttCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGcc                                                                                                                                                 <  1:779164/150‑1 (MQ=255)
        ccGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCAGATGCGGCGTAAACGCCtt                                                                                                                                               >  1:565581/1‑150 (MQ=255)
                gggCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCa                                                                                                                                       <  1:37764/150‑1 (MQ=255)
                gggCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATAAGGCGTTTACGCCGCATCCGGcc                                                                                                                                       <  1:1258352/150‑2 (MQ=255)
                  gCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCTTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATa                                                                                                                                     <  2:87224/150‑1 (MQ=255)
                       tGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATAAGGCGTTTACGCCGCATCCGGCCTattt                                                                                                                                  <  1:1193657/148‑4 (MQ=38)
                       tGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATAAGGCGTTTACGCCGCATCCGGCCTattt                                                                                                                                  >  2:1193657/1‑145 (MQ=38)
                        ggCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAttt                                                                                                                               <  1:303459/150‑1 (MQ=255)
                        ggCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAttt                                                                                                                               <  1:327494/150‑1 (MQ=255)
                        ggCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAttt                                                                                                                               <  2:28344/150‑1 (MQ=255)
                         gCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAtttt                                                                                                                              <  2:1412473/150‑1 (MQ=255)
                          cATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTa                                                                                                                             >  1:317994/1‑150 (MQ=255)
                            tttttCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTAtt                                                                                                                           <  2:1309926/150‑1 (MQ=255)
                              tttCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCg                                                                                                                         <  1:118956/150‑1 (MQ=255)
                                tCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAtttt                                                                                                                              >  1:771089/1‑143 (MQ=255)
                                tCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAtttt                                                                                                                              <  2:771089/143‑1 (MQ=255)
                                tCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGc                                                                                                                       >  2:743943/1‑150 (MQ=255)
                                 cTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAttt                                                                                                                               <  2:1210922/141‑1 (MQ=255)
                                 cTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAttt                                                                                                                               >  1:1210922/1‑141 (MQ=255)
                                      tcttcGCCCGTTTGACGCTGTTTAGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCt                                                                                                                 >  2:160884/1‑150 (MQ=255)
                                       cttcGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTc                                                                                                                >  2:15929/1‑150 (MQ=255)
                                              cGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCTAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGTCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAg                                                                                                         <  1:980041/150‑1 (MQ=255)
                                               gTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAgg                                                                                                        <  1:547972/150‑1 (MQ=255)
                                                 ttGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGcc                                                                                                      <  1:530084/150‑1 (MQ=255)
                                                  tGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCg                                                                                                     >  2:712684/1‑150 (MQ=255)
                                                  tGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCg                                                                                                     >  1:1094514/1‑150 (MQ=255)
                                                   gACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCgg                                                                                                    <  2:698320/150‑1 (MQ=255)
                                                    aCGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGa                                                                                                   <  1:20965/150‑1 (MQ=255)
                                                     cGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACgggg                                                                                                                                >  1:329116/1‑117 (MQ=255)
                                                     cGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACgggg                                                                                                                                <  2:323973/120‑4 (MQ=255)
                                                     cGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACgggg                                                                                                                                <  2:329116/120‑4 (MQ=255)
                                                     cGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACgggg                                                                                                                                >  1:323973/1‑117 (MQ=255)
                                                        tGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAg                                                                                               <  2:1375813/150‑1 (MQ=255)
                                                         gTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGa                                                                                              <  2:751012/150‑1 (MQ=255)
                                                         gTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGa                                                                                              >  1:446581/1‑150 (MQ=255)
                                                          ttttGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGAt                                                                                             >  1:652924/1‑150 (MQ=255)
                                                           tttGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCATAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATg                                                                                            <  1:1005122/150‑1 (MQ=255)
                                                               cgcgGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGcc                                                                                        <  2:586257/150‑1 (MQ=255)
                                                               cgcgGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGcc                                                                                        <  1:563618/150‑1 (MQ=255)
                                                                gcggcgTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCa                                                                                       <  2:317994/150‑1 (MQ=255)
                                                                  ggcgTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgc                                                                                     >  2:1428827/1‑150 (MQ=255)
                                                                  ggcgTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgc                                                                                     >  2:148991/1‑150 (MQ=255)
                                                                     gTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatc                                                                                  <  1:15929/150‑1 (MQ=255)
                                                                      tGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCTGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatcg                                                                                 <  2:565581/150‑1 (MQ=255)
                                                                      tGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatcg                                                                                 <  1:94972/150‑1 (MQ=255)
                                                                      gggATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatcg                                                                                 <  1:1046930/149‑1 (MQ=255)
                                                                             ccACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATCCGCCAGCATCGCAGCCgg                                                                          >  1:1098163/1‑150 (MQ=255)
                                                                              cACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATGCGATGCTGGCGCATCTTATCCGGc                                                                         <  2:964744/150‑1 (MQ=38)
                                                                              cACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGc                                                                         <  2:1094514/150‑1 (MQ=255)
                                                                                ccgccgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCta                                                                       <  2:368548/150‑1 (MQ=255)
                                                                                ccgccgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCta                                                                       <  1:567937/150‑1 (MQ=255)
                                                                                 cgccgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATGCGATGCTGGCGCATCTTATCCGGccta                                                                      <  2:931634/150‑4 (MQ=38)
                                                                                  gccgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCtata                                                                     >  2:1377763/1‑150 (MQ=255)
                                                                                   ccgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATaa                                                                    >  1:211444/1‑150 (MQ=255)
                                                                                      aaTATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGATCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCCTCCGGCTATAATgc                                                                 >  2:1278187/1‑150 (MQ=255)
                                                                                       atatAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATgcg                                                                >  2:616224/1‑150 (MQ=255)
                                                                                       atatAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCAGGCTATAATgcg                                                                >  2:605115/1‑150 (MQ=255)
                                                                                        tataAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATgcgc                                                               <  1:480073/150‑1 (MQ=255)
                                                                                          taAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGcac                                                             <  1:1180177/150‑1 (MQ=255)
                                                                                          taAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGcac                                                             >  1:1364405/1‑150 (MQ=255)
                                                                                           aaaGACGACCCGCGAATGCCGGGGAAAATGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGcaca                                                            <  1:306091/150‑1 (MQ=255)
                                                                                               acgacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAAc                                                        <  1:160884/150‑1 (MQ=255)
                                                                                                cgacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAAcc                                                       >  2:465688/1‑150 (MQ=255)
                                                                                                  acCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATACGCACATAACctc                                                     >  2:892781/1‑150 (MQ=255)
                                                                                                   cccGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACctct                                                    >  1:86866/1‑150 (MQ=255)
                                                                                                    ccGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGAGGCGGCGTAAACGCCTTATCCGGCAGACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCtt                                                   <  2:1327726/150‑1 (MQ=255)
                                                                                                     cgcgAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTg                                                  >  1:1114484/1‑150 (MQ=255)
                                                                                                     cgcgAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTg                                                  <  1:148991/150‑1 (MQ=255)
                                                                                                         aaTGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAAc                                              <  1:65069/150‑1 (MQ=255)
                                                                                                          aTGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACt                                             <  2:81597/150‑1 (MQ=255)
                                                                                                            gCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCg                                           <  2:462039/150‑1 (MQ=255)
                                                                                                            gCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCg                                           <  2:652924/150‑1 (MQ=255)
                                                                                                             ccGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGt                                          >  2:325891/1‑150 (MQ=255)
                                                                                                               ggggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTcc                                        >  1:260435/1‑150 (MQ=255)
                                                                                                               ggggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTcc                                        <  1:712684/150‑1 (MQ=255)
                                                                                                                             cTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCa                          <  1:465688/150‑1 (MQ=255)
                                                                                                                             cTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCa                          >  2:215529/1‑150 (MQ=255)
                                                                                                                              ttAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACATCTTGAAACTCGTCCCCAGAGCCTATTCac                         >  1:249641/1‑149 (MQ=255)
                                                                                                                               tAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAGTGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGc                        >  1:370788/1‑150 (MQ=255)
                                                                                                                                aaaaTAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGcc                       <  2:785199/150‑1 (MQ=255)
                                                                                                                                      tgCCGGATAAGGCGTTTACGCCGCATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTAt                 >  2:786115/2‑150 (MQ=39)
                                                                                                                                        ccGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTAttt               >  2:472099/1‑150 (MQ=255)
                                                                                                                                        ccGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTAttt               <  2:56610/150‑1 (MQ=255)
                                                                                                                                          ggataaggcgTTTACGCCGCATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGCGCCTCTTCAGCCCTCTATTTgg             >  2:790355/7‑150 (MQ=38)
                                                                                                                                           gATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTggg            <  2:17017/150‑1 (MQ=255)
                                                                                                                                              gcggcgTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGc         >  1:473143/1‑150 (MQ=255)
                                                                                                                                                ggcgTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGCGCCTCTTCAGCCATCTATTTGGGAGCaa       >  1:538452/1‑150 (MQ=255)
                                                                                                                                                 gcgTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCaaa      <  2:211444/150‑1 (MQ=255)
                                                                                                                                                 gcgTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCaaa      <  1:595887/150‑1 (MQ=255)
                                                                                                                                                    tAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACaa   >  1:301685/1‑150 (MQ=255)
                                                                                                                                                     aaaCGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAAt  >  1:967933/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTTGGTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAAT  >  NC_000913/3674246‑3674544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑