BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,779,929	C→T	6.0%	L35L (CTG→TTG)	lldD →	L‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,779,929	0	C	T	6.0%	538.3 / 14.4	168	L35L (CTG→TTG)	lldD	L‑lactate dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (76/81); new base T (7/3); total (84/84)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 171 total aligned reads displayed.

ACCCGCCTGCCCGGTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTA  >  NC_000913/3779780‑3780077

      cTGCCCGGTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGcgcc                                                                                                                                                <  1:1159905/150‑1 (MQ=255)

       tGCCCGGTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGcgccg                                                                                                                                               >  2:1171572/1‑150 (MQ=255)

            ggTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACg                                                                                                                                          >  2:285235/1‑150 (MQ=255)

               gAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTgg                                                                                                                                       >  1:1194700/1‑150 (MQ=255)

                 gCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCGTgtat                                                                                                                                     >  2:738512/1‑147 (MQ=255)

                 gCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCGTgtat                                                                                                                                     >  2:733610/1‑147 (MQ=255)

                 gCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGaa                                                                                                                                     >  1:507480/1‑150 (MQ=255)

                 gCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGaa                                                                                                                                     <  1:1083911/150‑1 (MQ=255)

                  cATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCGTgtatt                                                                                                                                    <  1:738512/150‑5 (MQ=255)

                     aaTGAGCATTCGATGGATAAAAACGCATTATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACTCTTTCTGCCGCCGTTCCTGTTCCACAATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGGGGAAGAtt                                                                                                                                 <  2:576152/150‑1 (MQ=255)

                      aTGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGAttt                                                                                                                                >  1:740438/1‑150 (MQ=255)

                         aGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTc                                                                                                                             <  1:136288/150‑1 (MQ=255)

                             ttCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGaa                                                                                                                         <  2:1317358/150‑1 (MQ=255)

                              tCGAGGGAGAAAAACGCATGATTATTTGCTCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAg                                                                                                                        <  2:845604/150‑1 (MQ=255)

                               cGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGt                                                                                                                       >  1:167528/1‑150 (MQ=255)

                                   ggAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGgcg                                                                                                                   <  2:1380643/150‑1 (MQ=255)

                                    gagaAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGgcgc                                                                                                                  <  1:1061284/150‑1 (MQ=255)

                                      gaAATACGCATGATTCTTTCCGCAGCGAGGGATTATCGAGCTGCAGCGCAACGCTTTCTGGCGACGTGCCTGTTCCACTAAATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGGCAGAAGTGGCGCTg                                                                                                                <  2:558156/150‑1 (MQ=255)

                                        aaaaCGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTgcg                                                                                                              >  1:1327756/1‑150 (MQ=255)

                                              cATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCg                                                                                                        <  2:1324527/150‑1 (MQ=255)

                                               aTGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGt                                                                                                       >  1:213414/1‑150 (MQ=255)

                                                   ttattTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTc                                                                                                   <  1:1031052/150‑1 (MQ=255)

                                                    tattTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCt                                                                                                  >  2:687312/1‑150 (MQ=255)

                                                        tCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGaaa                                                                                              <  2:1016775/150‑1 (MQ=255)

                                                          cGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGaaaaa                                                                                            <  1:701856/150‑1 (MQ=255)

                                                           gCAGCCAGCGATTATCGCGCCGCTTCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCCACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAAc                                                                                           <  1:684167/150‑1 (MQ=255)

                                                           gCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAAc                                                                                           <  2:167528/150‑1 (MQ=255)

                                                            cagccagcGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACa                                                                                          <  1:237941/150‑1 (MQ=255)

                                                              gccagcGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATg                                                                                        >  1:1314898/1‑150 (MQ=255)

                                                                       tATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTa                                                                               >  2:38113/1‑150 (MQ=255)

                                                                         tCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGa                                                                                                                          >  1:497344/1‑105 (MQ=255)

                                                                          cgcgCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGc                                                                            >  1:1252080/1‑150 (MQ=255)

                                                                           gcgcCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTGCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGcc                                                                           <  1:1401798/150‑1 (MQ=255)

                                                                             gccgcAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTg                                                                         <  2:297764/150‑1 (MQ=255)

                                                                              ccgcAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTgg                                                                        <  1:38113/150‑1 (MQ=255)

                                                                              ccgcAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTgg                                                                        <  2:459760/150‑1 (MQ=255)

                                                                                 cAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGaaa                                                                     >  2:883341/1‑150 (MQ=255)

                                                                                    cgcAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAacga                                                                  <  1:588557/150‑1 (MQ=255)

                                                                                     gcAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAacgac                                                                 <  1:299289/150‑1 (MQ=255)

                                                                                       aaCGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTACATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGc                                                               <  1:560644/150‑1 (MQ=255)

                                                                                         cGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTg                                                             <  2:193772/150‑1 (MQ=255)

                                                                                          gCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGt                                                            >  2:1029326/1‑150 (MQ=255)

                                                                                           cATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGtt                                                           >  1:292355/1‑150 (MQ=255)

                                                                                            aTTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGttt                                                          <  2:653255/150‑1 (MQ=255)

                                                                                            aTTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGttt                                                          <  1:803448/150‑1 (MQ=255)

                                                                                               cTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAAt                                                       <  2:292355/150‑1 (MQ=255)

                                                                                               cTGCAGCCGTAACTGTTACACTATATGGATGTTCGTGCATATTCTCAATACACGCTGCACCGAAAAGTGGAAGATTTGTCAAAAGTGGCGCAGCGCCAGCGTATTCTGAAAAAAATGTCCGACATAAAACTCGAAACGACCATGTTTaaa                                                       >  2:1116906/1‑149 (MQ=255)

                                                                                                tGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATg                                                      <  1:1428300/150‑1 (MQ=255)

                                                                                                tGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATg                                                      >  1:273817/1‑150 (MQ=255)

                                                                                                 gccgccGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATga                                                     >  1:1274813/1‑150 (MQ=255)

                                                                                                 gccgccGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATga                                                     <  1:495990/150‑1 (MQ=255)

                                                                                                 cccgcccTCCCTGTTCCACTAAATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATga                                                     <  1:642263/149‑1 (MQ=255)

                                                                                                  ccgccgTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATgag                                                    >  2:1338821/1‑150 (MQ=255)

                                                                                                         tCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATg                                                      >  2:995277/1‑141 (MQ=255)

                                                                                                         tCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATg                                                      <  1:995277/141‑1 (MQ=255)

                                                                                                           cTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCg                                           >  2:533373/1‑150 (MQ=255)

                                                                                                           cTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCg                                           >  1:1053054/1‑150 (MQ=255)

                                                                                                             gttaaaCTATATGGAAGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAACTGGCGCTGAGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGAt                                         >  2:1366708/6‑150 (MQ=255)

                                                                                                             gTTCCACTATATGGATGGTGGTGCATATTCTGAATACATGCTGCGCCGCAACGTTGAAGTTTTGTCAGAAGTGGCTCTGCGGCAGCTTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGAt                                         <  1:1116906/150‑1 (MQ=255)

                                                                                                             gTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGAt                                         <  2:332037/150‑1 (MQ=255)

                                                                                                                ccACTATATGGTTGGTGGTGCATATTCTGATTACACGCTGCGCCGCAACGTTGAAGATTTGTCAGAAGTGGCGCTGATCCAGCGTATTCTGAAATACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGca                                      <  1:658643/150‑2 (MQ=255)

                                                                                                                ccACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGcc                                      >  1:1308910/1‑150 (MQ=255)

                                                                                                                  aCTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCgg                                    >  1:1021589/1‑150 (MQ=255)

                                                                                                                   cTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCAGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGt                                   >  1:671061/1‑150 (MQ=255)

                                                                                                                    tataTGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTg                                  >  1:247655/1‑150 (MQ=255)

                                                                                                                      tatGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGc                                <  1:698908/150‑1 (MQ=255)

                                                                                                                      tatGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGc                                <  2:669727/150‑1 (MQ=255)

                                                                                                                         ggatggTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACt                             >  1:41630/1‑150 (MQ=255)

                                                                                                                          gatggTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTg                            >  2:801541/1‑150 (MQ=255)

                                                                                                                          gatggTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTg                            <  1:1147509/150‑1 (MQ=255)

                                                                                                                             ggtggtGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCt                         >  2:849640/1‑150 (MQ=255)

                                                                                                                              gtggtgCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGcc                                      >  2:1148460/1‑136 (MQ=255)

                                                                                                                                ggtgCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCg                      >  2:299362/1‑150 (MQ=255)

                                                                                                                                  tgCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGt                    <  2:1425166/150‑1 (MQ=255)

                                                                                                                                   gCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTg                   >  1:1235937/1‑150 (MQ=255)

                                                                                                                                   gCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTg                   >  2:1049834/1‑150 (MQ=255)

                                                                                                                                   gCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTg                   >  1:618814/1‑150 (MQ=255)

                                                                                                                                    cATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTgg                  <  2:1314898/150‑1 (MQ=255)

                                                                                                                                    cATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTgg                  >  1:897025/1‑150 (MQ=255)

                                                                                                                                     atatTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTggg                 >  1:185376/1‑150 (MQ=255)

                                                                                                                                       atTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGtt               >  2:1084203/1‑150 (MQ=255)

                                                                                                                                         tCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtg             <  2:213414/150‑1 (MQ=255)

                                                                                                                                           tGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTTGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGc                                <  1:574270/129‑1 (MQ=255)

                                                                                                                                           tGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtg           >  2:1132254/1‑150 (MQ=255)

                                                                                                                                            gAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtgt          >  1:1356989/1‑150 (MQ=255)

                                                                                                                                            gAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtgt          >  1:1352095/1‑150 (MQ=255)

                                                                                                                                             aaTACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtgtg         <  1:285235/150‑1 (MQ=255)

                                                                                                                                             aaTACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtgtg         >  2:425791/1‑150 (MQ=255)

                                                                                                                                              ttccacGTTGCGGCGCAGCGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTgg        >  2:15247/4‑150 (MQ=255)

                                                                                                                                               tccacGTTGCGGCGCAGCGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGc       >  1:1062655/3‑150 (MQ=255)

                                                                                                                                               tACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGc       <  1:639990/150‑1 (MQ=255)

                                                                                                                                                acacGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCa      <  2:1252080/150‑1 (MQ=255)

                                                                                                                                                 cacGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAGTTGTCGATGCCGGTGGCACTGGCt                         <  2:484052/130‑1 (MQ=255)

                                                                                                                                                 cacGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCAt     >  2:350335/1‑150 (MQ=255)

                                                                                                                                                  acGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATg    <  1:883341/150‑1 (MQ=255)

                                                                                                                                                   cGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGt   <  1:1423891/150‑1 (MQ=255)

                                                                                                                                                   cGCTGCGCCGAAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCTTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGt   >  2:1250594/1‑150 (MQ=255)

                                                                                                                                                    gCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCTGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTa  <  2:671061/150‑1 (MQ=255)

                                                                                                                                                    gCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTa  <  2:273817/150‑1 (MQ=255)

                                                                                                                                                    gCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTa  <  1:350335/150‑1 (MQ=255)

ACCCGCCTGCCCGGTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTA  >  NC_000913/3779780‑3780077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`