BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,779,934	C→G	6.1%	R36R (CGC→CGG)	lldD →	L‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,779,934	0	C	G	6.1%	566.9 / 19.9	165	R36R (CGC→CGG)	lldD	L‑lactate dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (75/80); new base G (7/3); total (82/83)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

Only 100 of 170 total aligned reads displayed.

CTGCCCGGTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTATGCGCG  >  NC_000913/3779786‑3780083

cTGCCCGGTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGcgcc                                                                                                                                                      <  1:1159905/150‑1 (MQ=255)

 tGCCCGGTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGcgccg                                                                                                                                                     >  2:1171572/1‑150 (MQ=255)

      ggTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCg                                                                                                                                                <  2:16557/150‑1 (MQ=255)

      ggTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACg                                                                                                                                                >  2:285235/1‑150 (MQ=255)

      ggTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACg                                                                                                                                                >  2:292059/1‑150 (MQ=255)

      ggTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACg                                                                                                                                                >  2:345757/1‑150 (MQ=255)

         gAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTgg                                                                                                                                             >  1:1194700/1‑150 (MQ=255)

         gAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTgg                                                                                                                                             <  1:259977/150‑1 (MQ=255)

           gCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCGTgtat                                                                                                                                           >  2:738512/1‑147 (MQ=255)

           gCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCGTgtat                                                                                                                                           >  2:733610/1‑147 (MQ=255)

           gCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGaa                                                                                                                                           <  1:1083911/150‑1 (MQ=255)

            cATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCGTgtatt                                                                                                                                          <  1:733610/150‑5 (MQ=255)

            cATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCGTgtatt                                                                                                                                          <  1:738512/150‑5 (MQ=255)

            cATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAg                                                                                                                                          <  2:870835/150‑1 (MQ=255)

                   aGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTc                                                                                                                                   <  1:136288/150‑1 (MQ=255)

                   aGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTc                                                                                                                                   <  1:1419996/150‑1 (MQ=255)

                       ttCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGaa                                                                                                                               >  1:207513/1‑150 (MQ=255)

                             ggAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGgcg                                                                                                                         <  2:1380643/150‑1 (MQ=255)

                              gagaAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGgcgc                                                                                                                        <  1:1061284/150‑1 (MQ=255)

                              gagaAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGgcgc                                                                                                                        >  2:1140374/1‑150 (MQ=255)

                              gagaAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGgcgc                                                                                                                        >  1:246817/1‑150 (MQ=255)

                                  aaaaCGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTgcg                                                                                                                    >  1:1327756/1‑150 (MQ=255)

                                  aaaaCGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTgcg                                                                                                                    <  2:353614/150‑1 (MQ=255)

                                       gCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGCCAGAAGTGGCGCTGCGCCAGc                                                                                                               <  1:1354804/150‑1 (MQ=255)

                                        cATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCg                                                                                                              <  2:1324527/150‑1 (MQ=255)

                                         aTGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGt                                                                                                             >  1:213414/1‑150 (MQ=255)

                                            attattTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTAtt                                                                                                          >  2:299289/1‑150 (MQ=255)

                                             ttattTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTc                                                                                                         <  1:1031052/150‑1 (MQ=255)

                                              tattTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCt                                                                                                        >  2:687312/1‑150 (MQ=255)

                                              tattTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCt                                                                                                        >  1:653255/1‑150 (MQ=255)

                                               attTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTg                                                                                                       <  2:447438/150‑1 (MQ=255)

                                                  tCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGaaa                                                                                                    <  2:1016775/150‑1 (MQ=255)

                                                     gCAGCCAGCGATTATCGCGCCGCTTCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCCACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAAc                                                                                                 <  1:684167/150‑1 (MQ=255)

                                                        gccagcGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATg                                                                                              >  1:1314898/1‑150 (MQ=255)

                                                          cagcGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTc                                                                                            <  1:345757/150‑1 (MQ=255)

                                                          cagcGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTc                                                                                            >  2:1428300/1‑150 (MQ=255)

                                                           agcGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTcc                                                                                           <  1:292059/150‑1 (MQ=255)

                                                                   tCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGa                                                                                                                                <  2:497344/105‑1 (MQ=255)

                                                                    cgcgCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGc                                                                                  >  1:1252080/1‑150 (MQ=255)

                                                                              cgcAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAacga                                                                        <  2:1327756/150‑1 (MQ=255)

                                                                                 aaCGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGc                                                                     >  1:499768/1‑150 (MQ=255)

                                                                                 aaCGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTACATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGc                                                                     <  1:560644/150‑1 (MQ=255)

                                                                                   cGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTg                                                                   <  2:193772/150‑1 (MQ=255)

                                                                                    gCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGt                                                                  >  2:1029326/1‑150 (MQ=255)

                                                                                    ccATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACa                                                                                                >  2:1055822/2‑120 (MQ=255)

                                                                                      aTTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGttt                                                                <  1:803448/150‑1 (MQ=255)

                                                                                      aTTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGttt                                                                <  1:558442/150‑1 (MQ=255)

                                                                                         cTGCAGCCGTAACTGTTACACTATATGGATGTTCGTGCATATTCTCAATACACGCTGCACCGAAAAGTGGAAGATTTGTCAAAAGTGGCGCAGCGCCAGCGTATTCTGAAAAAAATGTCCGACATAAAACTCGAAACGACCATGTTTaaa                                                             >  2:1116906/1‑149 (MQ=255)

                                                                                          tGCCTCCGTTCCTGTTCCACTATATGGATGGTTGTGCATATTCTGAATACACGCTTGGCCTCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACTACGCTGTTTAATg                                                            <  2:670324/150‑1 (MQ=255)

                                                                                          tGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATg                                                            >  1:273817/1‑150 (MQ=255)

                                                                                          tGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATg                                                            <  1:1428300/150‑1 (MQ=255)

                                                                                           gccgccGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATga                                                           <  1:495990/150‑1 (MQ=255)

                                                                                           gccgccGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATga                                                           >  1:1274813/1‑150 (MQ=255)

                                                                                           cccgcccTCCCTGTTCCACTAAATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATga                                                           <  1:642263/149‑1 (MQ=255)

                                                                                            ccgccgTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATgag                                                          >  2:642985/1‑150 (MQ=255)

                                                                                             cgccgTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATgaga                                                         >  1:689683/1‑150 (MQ=255)

                                                                                                   tCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATg                                                            >  2:995277/1‑141 (MQ=255)

                                                                                                     cTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCg                                                 >  1:1053054/1‑150 (MQ=255)

                                                                                                     cTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCg                                                 >  1:15148/1‑150 (MQ=255)

                                                                                                     cTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCg                                                 >  2:533373/1‑150 (MQ=255)

                                                                                                     cTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCg                                                 >  1:701166/1‑150 (MQ=255)

                                                                                                       gTTCCACTATATGGATGGTGGTGCATATTCTGAATACATGCTGCGCCGCAACGTTGAAGTTTTGTCAGAAGTGGCTCTGCGGCAGCTTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGAt                                               <  1:1116906/150‑1 (MQ=255)

                                                                                                       gTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGAt                                               <  2:332037/150‑1 (MQ=255)

                                                                                                          ccACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGcc                                            >  2:477674/1‑150 (MQ=255)

                                                                                                          ccACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGcc                                            >  1:1308910/1‑150 (MQ=255)

                                                                                                            aCTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCgg                                          >  1:1021589/1‑150 (MQ=255)

                                                                                                             cTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGt                                         <  2:1314997/150‑1 (MQ=255)

                                                                                                                tatGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGc                                      <  2:669727/150‑1 (MQ=255)

                                                                                                                 atggatggTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGCCa                                     <  2:1365810/150‑1 (MQ=255)

                                                                                                                  tggatggTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCAc                                    >  2:1167444/1‑150 (MQ=255)

                                                                                                                    gatggTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTg                                  <  1:1147509/150‑1 (MQ=255)

                                                                                                                    gatggTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTg                                  >  2:801541/1‑150 (MQ=255)

                                                                                                                       ggtggtGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCt                               >  2:849640/1‑150 (MQ=255)

                                                                                                                         tggtgCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTcc                             <  2:246817/150‑1 (MQ=255)

                                                                                                                          ggtgCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCg                            >  1:663854/1‑150 (MQ=255)

                                                                                                                             gCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTg                         >  1:1235937/1‑150 (MQ=255)

                                                                                                                             gCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTg                         >  1:618814/1‑150 (MQ=255)

                                                                                                                              cATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTgg                        >  1:897025/1‑150 (MQ=255)

                                                                                                                               atatTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTggg                       <  2:1274813/150‑1 (MQ=255)

                                                                                                                                 atTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGtt                     >  2:1007151/1‑150 (MQ=255)

                                                                                                                                 atTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGtt                     >  2:1084203/1‑150 (MQ=255)

                                                                                                                                 atTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGtt                     >  1:395963/1‑150 (MQ=255)

                                                                                                                                    cTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgt                  >  2:1423891/1‑150 (MQ=255)

                                                                                                                                     tGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGc                                      >  2:574270/1‑129 (MQ=255)

                                                                                                                                     tGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtg                 >  1:576144/1‑150 (MQ=255)

                                                                                                                                      gAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtgt                >  1:1352095/1‑150 (MQ=255)

                                                                                                                                       cttccacGTTGCGGCGCAGCGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtgtg               >  1:171229/5‑150 (MQ=255)

                                                                                                                                       aaTACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtgtg               >  2:425791/1‑150 (MQ=255)

                                                                                                                                         tccacGTTGCGGCGCAGCGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGc             >  1:1062655/3‑150 (MQ=255)

                                                                                                                                           cacGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAGTTGTCGATGCCGGTGGCACTGGCt                               >  1:484052/1‑130 (MQ=255)

                                                                                                                                            acGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATg          >  2:295290/1‑150 (MQ=255)

                                                                                                                                            acGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATg          <  1:883341/150‑1 (MQ=255)

                                                                                                                                             cGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGt         <  2:207513/150‑1 (MQ=255)

                                                                                                                                             cGCTGCGCCGAAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCTTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGt         >  2:1250594/1‑150 (MQ=255)

                                                                                                                                              gCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTa        <  1:350335/150‑1 (MQ=255)

                                                                                                                                              gCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTa        <  1:295290/150‑1 (MQ=255)

                                                                                                                                                tGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTATg      <  2:96685/150‑1 (MQ=255)

                                                                                                                                                  cgccgcAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTATgcg    >  1:977265/1‑150 (MQ=255)

                                                                                                                                                    ccgcAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTATGcgcg  <  1:784060/150‑1 (MQ=255)

                                                                                                                                                    ccgcAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTATGcgcg  <  2:1308910/150‑1 (MQ=255)

CTGCCCGGTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTATGCGCG  >  NC_000913/3779786‑3780083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`