BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,779,939	A→G	5.9%	N38S (AAC→AGC)	lldD →	L‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,779,939	0	A	G	5.9%	524.6 / 15.4	169	N38S (AAC→AGC)	lldD	L‑lactate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (77/82); new base G (7/3); total (84/85)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 169 total aligned reads displayed.

GGTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTATGCGCGT  >  NC_000913/3779792‑3780084

ggTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCg                                                                                                                                                 <  2:16557/150‑1 (MQ=255)

ggTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACg                                                                                                                                                 >  2:285235/1‑150 (MQ=255)

ggTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACg                                                                                                                                                 >  2:345757/1‑150 (MQ=255)

   gAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTgg                                                                                                                                              <  1:259977/150‑1 (MQ=255)

     gCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCGTgtat                                                                                                                                            >  2:738512/1‑147 (MQ=255)

     gCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCGTgtat                                                                                                                                            >  2:733610/1‑147 (MQ=255)

     gCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGaa                                                                                                                                            <  1:1083911/150‑1 (MQ=255)

     gCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGaa                                                                                                                                            >  1:507480/1‑150 (MQ=255)

      cATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGTTGCGGCGCAGCGTgtatt                                                                                                                                           <  1:738512/150‑5 (MQ=255)

      cATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAg                                                                                                                                           <  2:870835/150‑1 (MQ=255)

                 ttCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGaa                                                                                                                                >  1:207513/1‑150 (MQ=255)

                   cGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGt                                                                                                                              >  1:167528/1‑150 (MQ=255)

                       ggAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGgcg                                                                                                                          <  2:1380643/150‑1 (MQ=255)

                        gagaAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGgcgc                                                                                                                         <  1:1061284/150‑1 (MQ=255)

                          gaAATACGCATGATTCTTTCCGCAGCGAGGGATTATCGAGCTGCAGCGCAACGCTTTCTGGCGACGTGCCTGTTCCACTAAATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGGCAGAAGTGGCGCTg                                                                                                                       <  2:558156/150‑1 (MQ=255)

                            aaaaCGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTgcg                                                                                                                     <  2:353614/150‑1 (MQ=255)

                                 gCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGCCAGAAGTGGCGCTGCGCCAGc                                                                                                                <  1:1354804/150‑1 (MQ=255)

                                  cATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCg                                                                                                               <  2:1324527/150‑1 (MQ=255)

                                    tGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTa                                                                                                             <  1:1171572/150‑1 (MQ=255)

                                      attattTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTAtt                                                                                                           >  2:299289/1‑150 (MQ=255)

                                       ttattTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTc                                                                                                          <  1:1319346/150‑1 (MQ=255)

                                       ttattTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTc                                                                                                          <  1:1031052/150‑1 (MQ=255)

                                        tattTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCt                                                                                                         >  1:653255/1‑150 (MQ=255)

                                        tattTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCt                                                                                                         >  2:687312/1‑150 (MQ=255)

                                         attTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTg                                                                                                        <  2:447438/150‑1 (MQ=255)

                                            tCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGaaa                                                                                                     <  2:1016775/150‑1 (MQ=255)

                                              cGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGaaaaa                                                                                                   <  1:701856/150‑1 (MQ=255)

                                                cagccagcGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACa                                                                                                 <  1:237941/150‑1 (MQ=255)

                                                    cagcGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTc                                                                                             <  1:345757/150‑1 (MQ=255)

                                                    cagcGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTc                                                                                             >  2:1428300/1‑150 (MQ=255)

                                                             tCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGa                                                                                                                                 <  2:497344/105‑1 (MQ=255)

                                                             tCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGa                                                                                                                                 >  1:497344/1‑105 (MQ=255)

                                                               gcgcCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTGCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGcc                                                                                  <  1:1401798/150‑1 (MQ=255)

                                                                  ccgcAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTgg                                                                               <  1:38113/150‑1 (MQ=255)

                                                                        cgcAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAacga                                                                         <  1:588557/150‑1 (MQ=255)

                                                                         gcAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAacgac                                                                        <  1:299289/150‑1 (MQ=255)

                                                                         gcAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAacgac                                                                        >  1:121842/1‑150 (MQ=255)

                                                                             cGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTg                                                                    <  2:193772/150‑1 (MQ=255)

                                                                               cATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGtt                                                                  >  1:292355/1‑150 (MQ=255)

                                                                                aTTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGttt                                                                 <  1:558442/150‑1 (MQ=255)

                                                                                   cTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAAt                                                              <  2:292355/150‑1 (MQ=255)

                                                                                   cTGCAGCCGTAACTGTTACACTATATGGATGTTCGTGCATATTCTCAATACACGCTGCACCGAAAAGTGGAAGATTTGTCAAAAGTGGCGCAGCGCCAGCGTATTCTGAAAAAAATGTCCGACATAAAACTCGAAACGACCATGTTTaaa                                                              >  2:1116906/1‑149 (MQ=255)

                                                                                    tGCCTCCGTTCCTGTTCCACTATATGGATGGTTGTGCATATTCTGAATACACGCTTGGCCTCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACTACGCTGTTTAATg                                                             <  2:670324/150‑1 (MQ=255)

                                                                                    tGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATg                                                             <  1:1428300/150‑1 (MQ=255)

                                                                                      ccgccgTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATgag                                                           >  2:1338821/1‑150 (MQ=255)

                                                                                      ccgccgTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATgag                                                           >  2:642985/1‑150 (MQ=255)

                                                                                       cgccgTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATgaga                                                          >  1:689683/1‑150 (MQ=255)

                                                                                             tCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATg                                                             >  2:995277/1‑141 (MQ=255)

                                                                                             tCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATg                                                             <  1:995277/141‑1 (MQ=255)

                                                                                              ccTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACATGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTc                                                   >  2:1019563/1‑150 (MQ=255)

                                                                                               cTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCg                                                  <  1:787597/150‑1 (MQ=255)

                                                                                               cTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCg                                                  >  1:1053054/1‑150 (MQ=255)

                                                                                               cTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCg                                                  >  1:701166/1‑150 (MQ=255)

                                                                                               cTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCg                                                  <  2:120598/150‑1 (MQ=255)

                                                                                                 gTTCCACTATATGGATGGTGGTGCATATTCTGAATACATGCTGCGCCGCAACGTTGAAGTTTTGTCAGAAGTGGCTCTGCGGCAGCTTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGAt                                                <  1:1116906/150‑1 (MQ=255)

                                                                                                 gTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGAt                                                <  2:332037/150‑1 (MQ=255)

                                                                                                    ccACTATATGGTTGGTGGTGCATATTCTGATTACACGCTGCGCCGCAACGTTGAAGATTTGTCAGAAGTGGCGCTGATCCAGCGTATTCTGAAATACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGca                                             <  1:658643/150‑2 (MQ=255)

                                                                                                    ccACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGcc                                             >  1:1308910/1‑150 (MQ=255)

                                                                                                    ccACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGcc                                             >  2:477674/1‑150 (MQ=255)

                                                                                                      aCTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCgg                                           >  1:1021589/1‑150 (MQ=255)

                                                                                                       cTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGt                                          <  2:1314997/150‑1 (MQ=255)

                                                                                                       cTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCAGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGt                                          >  1:671061/1‑150 (MQ=255)

                                                                                                        tataTGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTg                                         <  1:1338821/150‑1 (MQ=255)

                                                                                                        tataTGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTg                                         >  1:247655/1‑150 (MQ=255)

                                                                                                         atatGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTgg                                        <  1:803982/150‑1 (MQ=255)

                                                                                                          tatGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGc                                       <  2:669727/150‑1 (MQ=255)

                                                                                                           atggatggTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGCCa                                      <  2:1365810/150‑1 (MQ=255)

                                                                                                             ggatggTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACt                                    >  1:41630/1‑150 (MQ=255)

                                                                                                              gatggTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTg                                   >  2:801541/1‑150 (MQ=255)

                                                                                                              gatggTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTg                                   <  1:1147509/150‑1 (MQ=255)

                                                                                                                 ggtggtGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCt                                >  2:849640/1‑150 (MQ=255)

                                                                                                                  gtggtgCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGcc                                             >  2:1148460/1‑136 (MQ=255)

                                                                                                                   tggtgCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTcc                              <  2:246817/150‑1 (MQ=255)

                                                                                                                      tgCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGt                           <  2:1425166/150‑1 (MQ=255)

                                                                                                                       gCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGCCGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTg                          >  1:1304108/1‑150 (MQ=255)

                                                                                                                       gCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTg                          >  1:618814/1‑150 (MQ=255)

                                                                                                                       gCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTg                          >  1:1235937/1‑150 (MQ=255)

                                                                                                                       gCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTg                          >  2:1049834/1‑150 (MQ=255)

                                                                                                                        cATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTgg                         <  2:1314898/150‑1 (MQ=255)

                                                                                                                        cATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTgg                         >  1:897025/1‑150 (MQ=255)

                                                                                                                         atatTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTggg                        <  2:1274813/150‑1 (MQ=255)

                                                                                                                         atatTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTggg                        >  1:185376/1‑150 (MQ=255)

                                                                                                                           atTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGtt                      >  2:1084203/1‑150 (MQ=255)

                                                                                                                            ttCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGttt                     >  1:1259958/1‑150 (MQ=255)

                                                                                                                             tCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtg                    <  2:213414/150‑1 (MQ=255)

                                                                                                                               tGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTTGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGc                                       <  1:574270/129‑1 (MQ=255)

                                                                                                                               tGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGc                                       >  2:574270/1‑129 (MQ=255)

                                                                                                                               tGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtg                  >  2:1132254/1‑150 (MQ=255)

                                                                                                                                gAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtgt                 >  1:1356989/1‑150 (MQ=255)

                                                                                                                                 aaTACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtgtg                >  2:425791/1‑150 (MQ=255)

                                                                                                                                 aaTACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTtgtgtg                <  1:285235/150‑1 (MQ=255)

                                                                                                                                   tccacGTTGCGGCGCAGCGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGc              >  1:1062655/3‑150 (MQ=255)

                                                                                                                                   tACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGc              <  1:639990/150‑1 (MQ=255)

                                                                                                                                     cacGTTGCGGCGCAGCGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCAt            >  1:976414/1‑150 (MQ=255)

                                                                                                                                     cacGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAGTTGTCGATGCCGGTGGCACTGGCt                                >  1:484052/1‑130 (MQ=255)

                                                                                                                                      acGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATg           >  2:295290/1‑150 (MQ=255)

                                                                                                                                       cGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGt          <  1:1423891/150‑1 (MQ=255)

                                                                                                                                       cGCTGCGCCGAAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCTTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGt          >  2:1250594/1‑150 (MQ=255)

                                                                                                                                          tGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTATg       <  2:96685/150‑1 (MQ=255)

                                                                                                                                              ccgcAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTATGcgcg   <  1:784060/150‑1 (MQ=255)

GGTGAGCATAATGAGCATTCGAGGGAGAAAAACGCATGATTATTTCCGCAGCCAGCGATTATCGCGCCGCAGCGCAACGCATTCTGCCGCCGTTCCTGTTCCACTATATGGATGGTGGTGCATATTCTGAATACACGCTGCGCCGCAACGTGGAAGATTTGTCAGAAGTGGCGCTGCGCCAGCGTATTCTGAAAAACATGTCCGACTTAAGCCTGGAAACGACGCTGTTTAATGAGAAATTGTCGATGCCGGTGGCACTGGCTCCGGTGGGTTTGTGTGGCATGTATGCGCGT  >  NC_000913/3779792‑3780084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`