BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,976,399	C→A	7.1%	A41E (GCA→GAA)	wzxE →	lipid III flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,976,399	0	C	A	7.1%	378.9 / 12.6	113	A41E (GCA→GAA)	wzxE	lipid III flippase
Reads supporting (aligned to +/- strand): ref base C (57/48); new base A (4/4); total (61/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

Only 100 of 122 total aligned reads displayed.

GCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCT  >  NC_000913/3976250‑3976548

  gACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCaa                                                                                                                                                      <  2:443182/150‑1 (MQ=255)

    cTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAAt                                                                                                                                                    <  2:579203/150‑1 (MQ=255)

    cTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAAt                                                                                                                                                    <  1:1238966/150‑1 (MQ=255)

        gttgAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTcc                                                                                                                                                <  2:1282822/150‑1 (MQ=255)

          tgAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGc                                                                                                                                              >  2:702006/1‑150 (MQ=255)

             actactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑g                                                                                                                                          >  1:347713/1‑150 (MQ=255)

             actactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑g                                                                                                                                          >  2:235435/1‑150 (MQ=255)

              ctactTTTCCTGATATGTCGTTGGCAAAAGCGGCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGGGCCGGGTGACTGGTCGGTAAGTTGCTGGCGGGGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGAAATTTGCCGCCA‑gc                                                                                                                                         <  1:1000793/150‑2 (MQ=255)

               tactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGAAATTTGCCGCCA‑gcc                                                                                                                                        <  1:686329/150‑3 (MQ=255)

               tactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGAAATTTGCCGCCA‑gcc                                                                                                                                        <  1:539126/150‑3 (MQ=255)

                actTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTg                                                                                                                                       >  2:643827/1‑150 (MQ=255)

                  ttttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGAt                                                                                                                                     <  1:839926/150‑1 (MQ=255)

                    ttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTa                                                                                                                                   <  1:428169/150‑1 (MQ=255)

                       cTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCg                                                                                                                                >  1:800447/1‑150 (MQ=255)

                            atGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTc                                                                                                                           >  2:362884/1‑150 (MQ=255)

                             tGTCGTTGGCAAAAGCGTCCTTGTGGATGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCg                                                                                                                          >  1:24548/1‑150 (MQ=255)

                             tGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCg                                                                                                                          <  2:515029/150‑1 (MQ=255)

                              gTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCgg                                                                                                                         >  2:922933/1‑150 (MQ=255)

                              gTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCgg                                                                                                                         >  2:191915/1‑150 (MQ=255)

                                cGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCg                                                                                                                       >  2:1278070/1‑150 (MQ=255)

                                   tGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGc                                                                                                                    >  1:322297/1‑150 (MQ=255)

                                     gCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCtt                                                                                                                  >  2:772612/1‑150 (MQ=255)

                                      cAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTg                                                                                                                 >  1:556978/1‑150 (MQ=255)

                                              tCCTTGTGGACGGCGGCCAGTACACTGGTCTAGTTTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCTTTTGGTCCGGCGGGGCTTTGTCTTGCGGCAAATTTCCGCGA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCt                                                                                                         <  2:179790/150‑1 (MQ=255)

                                              tCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCt                                                                                                         >  2:458241/1‑150 (MQ=255)

                                               ccTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGGCGGTAAGTTGCTGGCCGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTg                                                                                                        <  1:307533/150‑1 (MQ=255)

                                                cTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTgg                                                                                                       <  1:867760/150‑1 (MQ=255)

                                                  tgtgGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCa                                                                                                     <  1:643827/150‑1 (MQ=255)

                                                   gtgGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCAt                                                                                                    >  1:701435/1‑150 (MQ=255)

                                                    tgGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATc                                                                                                   <  2:347713/150‑1 (MQ=255)

                                                     ggACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCt                                                                                                  >  1:585476/1‑150 (MQ=255)

                                                      gACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCtt                                                                                                 >  1:1034895/1‑150 (MQ=255)

                                                       aCGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCttt                                                                                                >  2:274465/1‑150 (MQ=255)

                                                        cggcggCCAGTACACTGGTCAAGATTGGTTCCTTGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTTGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGTCGTGCTTGCCGGGGCTGGCATCTTTa                                                                                               <  2:780480/150‑1 (MQ=255)

                                                         ggcggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTaa                                                                                              >  1:1393129/1‑150 (MQ=255)

                                                          gcggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAAc                                                                                             >  1:762198/1‑150 (MQ=255)

                                                           cggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACg                                                                                            <  1:274465/150‑1 (MQ=255)

                                                            ggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTTGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACgg                                                                                           <  2:172105/150‑1 (MQ=255)

                                                            ggcCAGTACACTGGTCAAGATTGGGGCCGGGGTACTGGTCGGTAAGTTGCTGGCGGTGGCATTTGGTCCGGCGGGGCTTGGGCTGGCGCCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACgg                                                                                           <  1:941926/150‑1 (MQ=255)

                                                              ccAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgtg                                                                                         <  2:1390965/150‑1 (MQ=255)

                                                              ccAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgtg                                                                                         <  2:910280/150‑1 (MQ=255)

                                                                 gTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTaa                                                                                              >  2:742557/1‑142 (MQ=255)

                                                                    cacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTg                                                                                                                     <  1:43857/116‑1 (MQ=255)

                                                                    cacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCa                                                                                   >  2:1019055/1‑150 (MQ=255)

                                                                    cacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCa                                                                                   >  2:86923/1‑150 (MQ=255)

                                                                    cacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCa                                                                                   >  2:1075409/1‑150 (MQ=255)

                                                                        ggTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATa                                                                               <  1:1242227/150‑1 (MQ=255)

                                                                        ggTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATa                                                                               >  2:494777/1‑150 (MQ=255)

                                                                        ggTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATa                                                                               <  1:255214/150‑1 (MQ=255)

                                                                             aGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTg                                                                          >  2:792420/1‑150 (MQ=255)

                                                                                  ggTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCCTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAg                                                                     >  1:442387/1‑150 (MQ=255)

                                                                                    tGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGTTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTa                                                                   >  1:656466/1‑150 (MQ=255)

                                                                                    tGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTa                                                                   <  1:1058434/150‑1 (MQ=255)

                                                                                    tGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTa                                                                   >  1:1335558/1‑150 (MQ=255)

                                                                                     gCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCggg                                                                                                            >  2:983271/1‑108 (MQ=255)

                                                                                     gCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCggg                                                                                                            <  1:983271/108‑1 (MQ=255)

                                                                                     gCCGGGTAACTGGTCGGTAAGTTGCTGGCGGTCTCATTTGGTCCGGCGGGGATTGGGCTGGAGGCAAAATTCCGCCA‑GTTCATAACCGTGATCGGAGTGCTAGCCGCGCCTGGCATCTTTAAAGGAGTAACCAAATACGTTGCCCAGTAc                                                                  >  2:144975/1‑150 (MQ=255)

                                                                                      ccGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTAcc                                                                 >  1:757541/1‑150 (MQ=255)

                                                                                       ggggTTACTGGTCGGTAAGTTGCTGGCTGTGTCATTTGGTCCGGCGGGGCTTGGGCTTGCGGCAAATTTCCGCCA‑GTTGATTACCGTTCTCGGCGTTCTTGCCGTGGCTGGCATCTTTATCTGTGTAACGAAATACGTTGCCCAGTAGCa                                                                <  1:144975/149‑1 (MQ=255)

                                                                                       cGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCa                                                                >  2:287006/1‑150 (MQ=255)

                                                                                       cGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCa                                                                <  1:513569/150‑1 (MQ=255)

                                                                                         ggTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATg                                                              <  1:260399/150‑1 (MQ=255)

                                                                                          gTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGa                                                             <  2:299409/150‑1 (MQ=255)

                                                                                           ttACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGAt                                                            <  2:24548/150‑1 (MQ=255)

                                                                                             aCTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATaa                                                          >  1:1088369/1‑150 (MQ=255)

                                                                                               tGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATc                                                        >  2:461422/1‑150 (MQ=255)

                                                                                                ggTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATcc                                                       >  1:928135/1‑150 (MQ=255)

                                                                                                  tCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGc                                                     <  2:1393129/150‑1 (MQ=255)

                                                                                                   cGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCa                                                    >  2:153900/1‑150 (MQ=255)

                                                                                                       aaGTTGGTGGCGGGGTCTTTTGGTCGGGGGGGGCTTGGGCTGGCGGC‑AATTTTCGCCATTTTTATTACGGTGCTGGGGGTGCTTGCGGGGGGTGG‑ATTATTAACGGTGTAACCAAATACTTTGCCCTGTACCATGATAATCCGCAACAGc                                               <  2:1223831/150‑1 (MQ=255)

                                                                                                       aaGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAg                                                >  2:1360479/1‑150 (MQ=255)

                                                                                                            gCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTgcg                                           <  1:764582/150‑1 (MQ=255)

                                                                                                            gCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTgcg                                           >  2:749686/1‑150 (MQ=255)

                                                                                                             cTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATcc                                                       >  2:3094/1‑137 (MQ=255)

                                                                                                             cTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATcc                                                       <  1:3094/137‑1 (MQ=255)

                                                                                                               ggCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGcgccg                                        <  2:1301167/150‑1 (MQ=255)

                                                                                                                 cGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGc                                                     <  2:918239/135‑1 (MQ=255)

                                                                                                                  ggTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGt                                     <  1:792420/150‑1 (MQ=255)

                                                                                                                        aTTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCgg                               >  1:1194566/1‑150 (MQ=255)

                                                                                                                            ggTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACt                           >  2:1276762/1‑150 (MQ=255)

                                                                                                                             gTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACtt                          >  1:263291/1‑150 (MQ=255)

                                                                                                                             gTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACtt                          >  2:13273/1‑150 (MQ=255)

                                                                                                                               ccGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTtca                        >  1:809920/1‑150 (MQ=255)

                                                                                                                                 ggctgGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGTTGTAACCAAATACGTTGCCCAGTACCATGATAATCGGCAACAGCTGCTCCGCTTGGTCGGCACTtcatc                      <  2:482739/150‑1 (MQ=255)

                                                                                                                                    ggggCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGc                   <  2:762198/150‑1 (MQ=255)

                                                                                                                                         ttGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATgg              <  1:1290275/150‑1 (MQ=255)

                                                                                                                                          tGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGt             <  1:191915/150‑1 (MQ=255)

                                                                                                                                          tGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGt             >  1:421412/1‑150 (MQ=255)

                                                                                                                                            aacTGGCGGAAATTTGCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTAc           >  2:225679/3‑150 (MQ=255)

                                                                                                                                            aacTGGCGGAAATTTGCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTAc           >  2:1131983/3‑150 (MQ=255)

                                                                                                                                             gCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACt          >  2:54174/1‑150 (MQ=255)

                                                                                                                                             gCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACt          >  2:710149/1‑150 (MQ=255)

                                                                                                                                             acTGGCGGAAATTTGCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACt          >  2:1179048/2‑150 (MQ=255)

                                                                                                                                             acTGGCGGAAATTTGCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACt          >  1:16054/2‑150 (MQ=255)

                                                                                                                                               tGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTg        <  2:1194566/150‑1 (MQ=255)

                                                                                                                                                ggcggcAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTgg       <  2:1335558/150‑1 (MQ=255)

                                                                                                                                                ggcggcAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTgg       <  1:702006/150‑1 (MQ=255)

                                                                                                                                                  cggcAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGtt     <  1:458241/150‑1 (MQ=255)

                                                                                                                                                    gcAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTtc   <  1:1075409/150‑1 (MQ=255)

                                                                                                                                                     cAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTtct  >  1:745133/1‑150 (MQ=255)

GCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCT  >  NC_000913/3976250‑3976548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`