BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,976,405	T→G	6.7%	F43C (TTC→TGC)	wzxE →	lipid III flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,976,405	0	T	G	6.7%	377.8 / 16.6	119	F43C (TTC→TGC)	wzxE	lipid III flippase
Reads supporting (aligned to +/- strand): ref base T (59/52); new base G (4/4); total (63/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

Only 100 of 123 total aligned reads displayed.

GTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGC  >  NC_000913/3976258‑3976554

gttgAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTcc                                                                                                                                                      <  2:1282822/150‑1 (MQ=255)

     actactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑g                                                                                                                                                >  1:347713/1‑150 (MQ=255)

     actactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑g                                                                                                                                                >  2:235435/1‑150 (MQ=255)

      ctactTTTCCTGATATGTCGTTGGCAAAAGCGGCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGGGCCGGGTGACTGGTCGGTAAGTTGCTGGCGGGGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGAAATTTGCCGCCA‑gc                                                                                                                                               <  1:1000793/150‑2 (MQ=255)

       tactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGAAATTTGCCGCCA‑gcc                                                                                                                                              <  1:539126/150‑3 (MQ=255)

       tactTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGAAATTTGCCGCCA‑gcc                                                                                                                                              <  1:686329/150‑3 (MQ=255)

        actTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTg                                                                                                                                             >  2:643827/1‑150 (MQ=255)

          ttttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGAt                                                                                                                                           <  1:839926/150‑1 (MQ=255)

          ttttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGAt                                                                                                                                           >  1:637692/1‑150 (MQ=255)

            ttCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTa                                                                                                                                         <  1:428169/150‑1 (MQ=255)

               cTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCg                                                                                                                                      >  1:800447/1‑150 (MQ=255)

               cTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCg                                                                                                                                      >  1:1390965/1‑150 (MQ=255)

                    atGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTc                                                                                                                                 >  2:362884/1‑150 (MQ=255)

                     tGTCGTTGGCAAAAGCGTCCTTGTGGATGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCg                                                                                                                                >  1:24548/1‑150 (MQ=255)

                     tGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCg                                                                                                                                <  2:515029/150‑1 (MQ=255)

                      gTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCgg                                                                                                                               >  2:922933/1‑150 (MQ=255)

                        cGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCg                                                                                                                             >  2:1278070/1‑150 (MQ=255)

                           tGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGc                                                                                                                          >  1:322297/1‑150 (MQ=255)

                             gCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCtt                                                                                                                        >  2:772612/1‑150 (MQ=255)

                              cAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTg                                                                                                                       >  1:402146/1‑150 (MQ=255)

                                      tCCTTGTGGACGGCGGCCAGTACACTGGTCTAGTTTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCTTTTGGTCCGGCGGGGCTTTGTCTTGCGGCAAATTTCCGCGA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCt                                                                                                               <  2:179790/150‑1 (MQ=255)

                                      tCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCt                                                                                                               >  2:458241/1‑150 (MQ=255)

                                       ccTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGGCGGTAAGTTGCTGGCCGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTg                                                                                                              <  1:307533/150‑1 (MQ=255)

                                        cTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTgg                                                                                                             <  1:867760/150‑1 (MQ=255)

                                         ttGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGATTGCCGGGGCTGGc                                                                                                            >  1:494555/1‑150 (MQ=255)

                                          tgtgGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCa                                                                                                           <  1:643827/150‑1 (MQ=255)

                                           gtgGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCAt                                                                                                          >  1:701435/1‑150 (MQ=255)

                                            tgGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATc                                                                                                         <  2:347713/150‑1 (MQ=255)

                                             ggACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCt                                                                                                        >  1:585476/1‑150 (MQ=255)

                                              gACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCtt                                                                                                       >  1:1034895/1‑150 (MQ=255)

                                               aCGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCttt                                                                                                      >  2:274465/1‑150 (MQ=255)

                                                cggcggCCAGTACACTGGTCAAGATTGGTTCCTTGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTTGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGTCGTGCTTGCCGGGGCTGGCATCTTTa                                                                                                     <  2:780480/150‑1 (MQ=255)

                                                 ggcggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTaa                                                                                                    >  1:1393129/1‑150 (MQ=255)

                                                  gcggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAAc                                                                                                   >  1:762198/1‑150 (MQ=255)

                                                    ggcCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTTGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACgg                                                                                                 <  2:172105/150‑1 (MQ=255)

                                                    ggcCAGTACACTGGTCAAGATTGGGGCCGGGGTACTGGTCGGTAAGTTGCTGGCGGTGGCATTTGGTCCGGCGGGGCTTGGGCTGGCGCCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACgg                                                                                                 <  1:941926/150‑1 (MQ=255)

                                                      ccAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgtg                                                                                               <  2:1390965/150‑1 (MQ=255)

                                                      ccAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgtg                                                                                               <  2:910280/150‑1 (MQ=255)

                                                         gTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTaa                                                                                                    >  2:742557/1‑142 (MQ=255)

                                                         gTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTaa                                                                                                    <  1:742557/142‑1 (MQ=255)

                                                          tACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTGCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAAc                                                                                           <  2:1329512/150‑1 (MQ=255)

                                                          tACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAAc                                                                                           <  2:1034895/150‑1 (MQ=255)

                                                            cacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTg                                                                                                                           <  1:43857/116‑1 (MQ=255)

                                                            cacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCa                                                                                         >  2:86923/1‑150 (MQ=255)

                                                            cacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCa                                                                                         >  2:1019055/1‑150 (MQ=255)

                                                               tGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAAt                                                                                      <  1:772612/150‑1 (MQ=255)

                                                                ggTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATa                                                                                     <  1:255214/150‑1 (MQ=255)

                                                                ggTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATa                                                                                     <  1:1242227/150‑1 (MQ=255)

                                                                     aGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTg                                                                                >  2:792420/1‑150 (MQ=255)

                                                                        ttGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTTCTCGGCGTGCTTGCCGGTGCTGGAATCTTTAACGGTGTAACCAAATAGGTTgact                                                                             <  2:701435/150‑4 (MQ=255)

                                                                            tGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGTTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTa                                                                         >  1:656466/1‑150 (MQ=255)

                                                                            tGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTa                                                                         <  1:1058434/150‑1 (MQ=255)

                                                                             gCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCggg                                                                                                                  >  2:983271/1‑108 (MQ=255)

                                                                             gCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCggg                                                                                                                  <  1:983271/108‑1 (MQ=255)

                                                                             gCCGGGTAACTGGTCGGTAAGTTGCTGGCGGTCTCATTTGGTCCGGCGGGGATTGGGCTGGAGGCAAAATTCCGCCA‑GTTCATAACCGTGATCGGAGTGCTAGCCGCGCCTGGCATCTTTAAAGGAGTAACCAAATACGTTGCCCAGTAc                                                                        >  2:144975/1‑150 (MQ=255)

                                                                              ccGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTAcc                                                                       >  1:757541/1‑150 (MQ=255)

                                                                               ggggTTACTGGTCGGTAAGTTGCTGGCTGTGTCATTTGGTCCGGCGGGGCTTGGGCTTGCGGCAAATTTCCGCCA‑GTTGATTACCGTTCTCGGCGTTCTTGCCGTGGCTGGCATCTTTATCTGTGTAACGAAATACGTTGCCCAGTAGCa                                                                      <  1:144975/149‑1 (MQ=255)

                                                                               cGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCa                                                                      >  2:287006/1‑150 (MQ=255)

                                                                               cGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCa                                                                      <  1:513569/150‑1 (MQ=255)

                                                                                 ggTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATg                                                                    <  1:260399/150‑1 (MQ=255)

                                                                                  gTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGa                                                                   <  2:299409/150‑1 (MQ=255)

                                                                                   ttACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGAt                                                                  <  2:24548/150‑1 (MQ=255)

                                                                                     aCTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATaa                                                                >  1:1088369/1‑150 (MQ=255)

                                                                                       tGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATc                                                              >  2:461422/1‑150 (MQ=255)

                                                                                        ggTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATcc                                                             >  1:285506/1‑150 (MQ=255)

                                                                                        ggTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATcc                                                             >  1:928135/1‑150 (MQ=255)

                                                                                          tCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGc                                                           <  2:1393129/150‑1 (MQ=255)

                                                                                           cGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCa                                                          >  2:153900/1‑150 (MQ=255)

                                                                                               aaGTTGGTGGCGGGGTCTTTTGGTCGGGGGGGGCTTGGGCTGGCGGC‑AATTTTCGCCATTTTTATTACGGTGCTGGGGGTGCTTGCGGGGGGTGG‑ATTATTAACGGTGTAACCAAATACTTTGCCCTGTACCATGATAATCCGCAACAGc                                                     <  2:1223831/150‑1 (MQ=255)

                                                                                               aaGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAg                                                      >  2:1360479/1‑150 (MQ=255)

                                                                                                    gCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTgcg                                                 <  1:764582/150‑1 (MQ=255)

                                                                                                    gCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTgcg                                                 >  2:749686/1‑150 (MQ=255)

                                                                                                     cTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATcc                                                             >  2:3094/1‑137 (MQ=255)

                                                                                                     cTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATcc                                                             <  1:3094/137‑1 (MQ=255)

                                                                                                       ggCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGcgccg                                              <  2:1301167/150‑1 (MQ=255)

                                                                                                         cGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGc                                                           <  2:918239/135‑1 (MQ=255)

                                                                                                                aTTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCgg                                     >  1:1194566/1‑150 (MQ=255)

                                                                                                                    ggTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACt                                 >  2:1276762/1‑150 (MQ=255)

                                                                                                                     gTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACtt                                >  1:263291/1‑150 (MQ=255)

                                                                                                                       ccGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTtca                              >  1:809920/1‑150 (MQ=255)

                                                                                                                         ggctgGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGTTGTAACCAAATACGTTGCCCAGTACCATGATAATCGGCAACAGCTGCTCCGCTTGGTCGGCACTtcatc                            <  2:482739/150‑1 (MQ=255)

                                                                                                                                 ttGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATgg                    <  1:1290275/150‑1 (MQ=255)

                                                                                                                                  tGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGt                   <  1:191915/150‑1 (MQ=255)

                                                                                                                                  tGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGt                   >  1:421412/1‑150 (MQ=255)

                                                                                                                                    aacTGGCGGAAATTTGCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTAc                 >  2:225679/3‑150 (MQ=255)

                                                                                                                                    aacTGGCGGAAATTTGCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTAc                 >  2:1131983/3‑150 (MQ=255)

                                                                                                                                     gCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACt                >  2:54174/1‑150 (MQ=255)

                                                                                                                                     gCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACt                >  2:710149/1‑150 (MQ=255)

                                                                                                                                     acTGGCGGAAATTTGCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACt                >  1:16054/2‑150 (MQ=255)

                                                                                                                                     acTGGCGGAAATTTGCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACt                >  2:1179048/2‑150 (MQ=255)

                                                                                                                                       tGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTg              <  2:1194566/150‑1 (MQ=255)

                                                                                                                                        ggcggcAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTgg             <  1:702006/150‑1 (MQ=255)

                                                                                                                                        ggcggcAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTgg             <  2:1335558/150‑1 (MQ=255)

                                                                                                                                          cggcAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGtt           <  1:458241/150‑1 (MQ=255)

                                                                                                                                            gcAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTtc         <  2:757541/150‑1 (MQ=255)

                                                                                                                                            gcAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTtc         <  1:1075409/150‑1 (MQ=255)

                                                                                                                                            gcAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGATTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTtc         <  2:494555/150‑1 (MQ=255)

                                                                                                                                                 tttCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTAc    <  1:1278070/150‑1 (MQ=255)

                                                                                                                                                  ttCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCg                        >  2:737087/1‑129 (MQ=255)

                                                                                                                                                  ttCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCg                        <  1:737087/129‑1 (MQ=255)

GTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCA‑GTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGC  >  NC_000913/3976258‑3976554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`