Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,317,977 A→G 5.3% intergenic (‑82/+29) phnL ← / ← phnK methylphosphonate degradation complex subunit PhnL/carbon‑phosphorus lyase subunit PhnK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,317,9770AG 5.3% 527.1 / 13.3 168intergenic (‑82/+29)phnL/phnKmethylphosphonate degradation complex subunit PhnL/carbon‑phosphorus lyase subunit PhnK
Reads supporting (aligned to +/- strand):  ref base A (69/90);  new base G (3/6);  total (72/96)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 171 total aligned reads displayed.

CGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCC  >  NC_000913/4317829‑4318126
                                                                                                                                                    |                                                                                                                                                     
   gCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGc                                                                                                                                                   <  2:1383581/150‑1 (MQ=255)
       gcgcACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGttt                                                                                                                                               <  2:565637/150‑1 (MQ=255)
           aCGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGATGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                           >  2:874886/1‑150 (MQ=255)
            cGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                          <  2:214667/150‑1 (MQ=255)
            cGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                          >  2:73149/1‑150 (MQ=255)
             gCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                         <  1:1172982/150‑1 (MQ=255)
              ccGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                        >  1:1168956/1‑150 (MQ=255)
                gTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAAcgccttat                                                                                                                                      >  2:1070146/1‑146 (MQ=38)
                    tGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                  <  1:1133003/150‑1 (MQ=37)
                     gCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                 <  2:444210/150‑1 (MQ=255)
                      cTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                <  1:284484/150‑2 (MQ=37)
                      cTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                                >  1:845287/1‑150 (MQ=255)
                       tGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                                                                                                               <  2:942290/150‑3 (MQ=37)
                         gTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcctac                                                                                                                             <  1:1070146/150‑5 (MQ=37)
                           gCAGTATGTAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcctacaa                                                                                                                           <  2:1120531/150‑7 (MQ=25)
                           gCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGc                                                                                                                           >  2:645861/1‑150 (MQ=255)
                            cAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCCTCCGGCACCGGCt                                                                                                                          >  1:1229432/1‑150 (MQ=255)
                            cAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCt                                                                                                                          <  2:157127/150‑1 (MQ=255)
                             aGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTc                                                                                                                         <  2:536927/150‑1 (MQ=255)
                              ggATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGTTTCGGGTGTCGGATGCGAAGCTGACGCGTCTTATCAGACCTACGGGGAGCGCATTTGTATGCCGGATAAGTCGTTTACGCCGCATCCGGCACCGGCTCa                                                                                                                        >  2:666322/1‑150 (MQ=255)
                                aTGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAt                                                                                                                      <  1:32863/150‑1 (MQ=255)
                                    cggTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTg                                                                                                                  <  1:1383193/149‑1 (MQ=255)
                                    aGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTg                                                                                                                  <  1:887604/150‑1 (MQ=255)
                                    aGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTg                                                                                                                  <  2:1411047/150‑1 (MQ=255)
                                    aGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTg                                                                                                                  >  1:254298/1‑150 (MQ=255)
                                     ggTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGc                                                                                                                 <  2:950480/150‑1 (MQ=255)
                                         ttACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCaaaa                                                                                                             >  2:585471/1‑150 (MQ=255)
                                             tGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTACGGGTGTCGGATGCGACGCTGACGCGTCTTATCTTACATACGTGGAGCGCATTTGTAGGCCTGATAAGGCGTTTACGCCGCATACGTCACCGGCTCAATTCTGCAAAACAGa                                                                                                         <  2:692827/150‑1 (MQ=255)
                                              gACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGc                                                                                                                 >  2:252301/1‑141 (MQ=255)
                                              gACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                        >  1:1333850/1‑150 (MQ=255)
                                              gACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                        >  1:970074/1‑150 (MQ=255)
                                              gACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                        >  2:799187/1‑150 (MQ=255)
                                              gACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                        <  2:772705/150‑1 (MQ=255)
                                              gACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                        >  1:861453/1‑150 (MQ=255)
                                                    ttGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACAcc                                                                                                  <  2:1148443/150‑1 (MQ=255)
                                                      gTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcag                                                                                                >  1:1076919/1‑150 (MQ=255)
                                                      gTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcag                                                                                                <  1:1390918/150‑1 (MQ=255)
                                                         cGTTATTCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagcag                                                                                             >  2:412010/1‑150 (MQ=255)
                                                         cGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTg                                                                                                                  >  1:178138/1‑129 (MQ=255)
                                                         cGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTg                                                                                                                  <  2:178138/129‑1 (MQ=255)
                                                         cGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagcag                                                                                             >  1:687579/1‑150 (MQ=255)
                                                         cGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagcag                                                                                             >  2:157349/1‑150 (MQ=255)
                                                          gTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCagcagc                                                                                            <  2:1260038/150‑1 (MQ=255)
                                                            tAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtg                                                                                          <  1:66609/150‑1 (MQ=255)
                                                             aaTCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgt                                                                                         >  1:85772/1‑150 (MQ=255)
                                                               tCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATg                                                                                                       >  1:1028970/1‑134 (MQ=255)
                                                               tCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtg                                                                                          >  1:974971/1‑147 (MQ=255)
                                                                  tttttCGCCTCGTGGGTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCACCGGCTCATTTCTGCAAAACCTATGACACCAGCAGCTGTGtata                                                                                    <  2:201202/150‑1 (MQ=255)
                                                                       cGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGgat                                                                               >  2:1350767/1‑150 (MQ=255)
                                                                          cTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGgatgat                                                                            <  1:927145/150‑1 (MQ=255)
                                                                            cGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGc                                                                          >  2:241199/1‑150 (MQ=255)
                                                                             gTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCg                                                                         <  1:241199/150‑1 (MQ=255)
                                                                               ggTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCTATGACACCAGCAGCTGTGTATACGTATGATGCtgg                                                                       <  2:1232747/150‑1 (MQ=255)
                                                                                   cGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGgtcgt                                                                   >  1:1303685/1‑150 (MQ=255)
                                                                                     ggTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGtcgtcg                                                                 <  2:602532/150‑1 (MQ=255)
                                                                                       tgtCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAg                                                               <  2:970074/150‑1 (MQ=255)
                                                                                       tgtCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAg                                                               <  2:687579/150‑1 (MQ=255)
                                                                                             aTGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCAcgcg                                                         <  2:1174162/150‑1 (MQ=255)
                                                                                             aTGCGACGCTGACGCGCCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCatacg                                                         <  2:175012/150‑5 (MQ=255)
                                                                                               gCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggt                                                       >  2:1387075/1‑150 (MQ=255)
                                                                                               gCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggt                                                       <  1:799187/150‑1 (MQ=255)
                                                                                                 gACGCTCACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCCTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAAGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtcg                                                     >  2:140336/1‑150 (MQ=255)
                                                                                                     cTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTa                                                 <  2:141695/150‑1 (MQ=255)
                                                                                                       gACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAAc                                               <  1:666322/150‑1 (MQ=255)
                                                                                                           cgtcgcaTCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCAc                                           <  1:820443/144‑1 (MQ=255)
                                                                                                           cgTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGGTGCGGGTCGTCGAGCACGCGGTCGGTTAACCCAc                                           >  1:435147/1‑150 (MQ=255)
                                                                                                              cTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCActct                                        >  2:1018721/1‑150 (MQ=255)
                                                                                                                tATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTcc                                      >  1:1362998/1‑150 (MQ=255)
                                                                                                                tATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTcc                                      <  2:256656/150‑1 (MQ=255)
                                                                                                                tATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTcc                                      <  2:1333850/150‑1 (MQ=255)
                                                                                                                 aTCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTcca                                     <  2:1362998/150‑1 (MQ=255)
                                                                                                                   ccGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTccacc                                   <  1:585471/150‑1 (MQ=255)
                                                                                                                     gACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCcaccac                                 <  2:154259/150‑1 (MQ=255)
                                                                                                                     gACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCcaccac                                 >  2:1258649/1‑150 (MQ=255)
                                                                                                                         tACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGc                             >  2:509072/1‑150 (MQ=255)
                                                                                                                         tACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGc                             >  1:340434/1‑150 (MQ=255)
                                                                                                                         tACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGc                             >  1:1155272/1‑150 (MQ=255)
                                                                                                                          aCGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGcc                            >  1:1001386/1‑150 (MQ=255)
                                                                                                                             gggAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCt                         <  2:1349159/150‑1 (MQ=255)
                                                                                                                             gggAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCt                         <  1:850412/150‑1 (MQ=255)
                                                                                                                                   gcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtca                   <  1:1350767/150‑1 (MQ=255)
                                                                                                                                   gcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtca                   <  1:447876/150‑1 (MQ=255)
                                                                                                                                   gcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtca                   >  2:106834/1‑150 (MQ=255)
                                                                                                                                   gcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTACTtca                   <  2:1371534/150‑1 (MQ=255)
                                                                                                                                     aTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcatc                 <  1:536507/150‑1 (MQ=255)
                                                                                                                                       ttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCAc               <  1:1431417/150‑1 (MQ=255)
                                                                                                                                       ttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCAc               <  1:1426716/150‑1 (MQ=255)
                                                                                                                                       ttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCAc               >  1:1295076/1‑150 (MQ=255)
                                                                                                                                        tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCAcc              >  2:1122172/1‑150 (MQ=255)
                                                                                                                                         gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCa             <  2:839893/150‑1 (MQ=255)
                                                                                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCa             <  1:1039058/147‑1 (MQ=255)
                                                                                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCa             >  2:1039058/1‑147 (MQ=255)
                                                                                                                                             gCCGGATGCGGCGTAAACGCCTTATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCaa         >  2:368729/1‑150 (MQ=255)
                                                                                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCaa         >  1:956082/1‑150 (MQ=255)
                                                                                                                                               cGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAAc       <  2:340434/150‑1 (MQ=255)
                                                                                                                                               cGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAAc       <  1:901281/150‑1 (MQ=255)
                                                                                                                                                ggATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACg      >  1:195533/1‑150 (MQ=255)
                                                                                                                                                 gATAAGGCGTTTACGCCGCATCCGGCTCCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACgg     >  2:788511/1‑150 (MQ=255)
                                                                                                                                                 gATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACgg     <  1:354423/150‑1 (MQ=255)
                                                                                                                                                    aaGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTcc  <  2:531465/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCC  >  NC_000913/4317829‑4318126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑