Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	436,696	T→C	7.4%	V36V (GTT→GTC)	pgpA →	phosphatidylglycerophosphatase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	436,696	0	T	C	7.4%	192.0 / 5.5	67	V36V (GTT→GTC)	pgpA	phosphatidylglycerophosphatase A
Reads supporting (aligned to +/- strand):  ref base T (36/26);  new base C (2/3);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

TTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGGTGT  >  NC_000913/436547‑436839                                                                                                                                                      |                                                                                                                                                 ttATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGtt                                                                                                                                                  >  2:708436/1‑150 (MQ=255) ttATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGtt                                                                                                                                                  <  2:1158271/150‑1 (MQ=255)   aTTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTcc                                                                                                                                                >  1:1089460/1‑150 (MQ=255)    ttCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCt                                                                                                                                               >  1:623045/1‑150 (MQ=255)     tCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTg                                                                                                                                              <  2:949433/150‑1 (MQ=255)       gTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTggg                                                                                                                                            >  1:641665/1‑150 (MQ=255)        tGACGGCGAACCTGTTACATTAGACTGGAAAGGAAATGACCATTTTGCCACGCCATAAAGATTTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGAGGCATCTACTTGCTGTCGGATTCGGAACTGGATTAAGGCCGATCGTTCCTCGGa                                                                                                                                           >  2:789979/1‑150 (MQ=255)                aaCCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCC‑ATCGTCCCAGGAACGAT‑cgggc                                                                                                                                 <  1:953/150‑3 (MQ=255)                 aCCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCt                                                                                                                                 <  2:1365614/150‑1 (MQ=255)                 aCCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCC‑ATCGTCCCAGGAACGATCGGGCt                                                                                                                                 <  2:858171/150‑1 (MQ=255)                  ccTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCC‑ATCGTCCCAGGAACGATCGGGCtt                                                                                                                                <  1:650620/150‑2 (MQ=255)                     gTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCt                                                                                                                             <  2:670942/150‑1 (MQ=255)                       tACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTgg                                                                                                                           >  2:723645/1‑150 (MQ=255)                        aCATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGc                                                                                                                          >  2:567089/1‑150 (MQ=255)                          aTTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAg                                                                                                                        <  2:14317/150‑1 (MQ=255)                          aTTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAg                                                                                                                        <  2:1426574/150‑1 (MQ=255)                             aGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGa                                                                                                                     >  1:205639/1‑150 (MQ=255)                                cTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTc                                                                                                                  >  1:1325229/1‑150 (MQ=255)                                        gATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑g                                                                                                                                     <  2:1012577/123‑1 (MQ=255)                                        gATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑g                                                                                                                                     >  1:1012577/1‑123 (MQ=255)                                        gATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTgg                                                                                                          <  2:223072/150‑1 (MQ=255)                                            tGACCTTTTTGCGACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGc                                                                                                                       <  1:955562/133‑1 (MQ=255)                                            tGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGc                                                                                                                       >  2:955562/1‑133 (MQ=255)                                            tGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGt                                                                                                               >  2:659949/1‑141 (MQ=255)                                            tGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGt                                                                                                               <  1:659949/141‑1 (MQ=255)                                                  ttttGCCACGCCATAATGATGTCGCGAAATGTCGCCTGAAGATGAGTAATCGGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCGCGTTCGTTCCTGGGTCGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCtgatga                                                                                                <  2:991045/150‑1 (MQ=255)                                                    ttGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGAcc                                                                                              >  2:958991/1‑150 (MQ=255)                                                       ccACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttt                                                                                           >  1:510815/1‑150 (MQ=255)                                                        cACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCtttt                                                                                          >  2:1396252/1‑150 (MQ=255)                                                           gCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGc                                                                                       <  1:723645/150‑1 (MQ=255)                                                              aTAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCt                                                                                    >  2:461715/1‑150 (MQ=255)                                                              aTAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCt                                                                                    >  1:259525/1‑150 (MQ=255)                                                                 aaGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGc                                                                                 >  1:650273/1‑150 (MQ=255)                                                                 aaGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGGCGGATTCGGAAGTGGATTAAGCCCGAGCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGc                                                                                 <  1:1252892/150‑1 (MQ=255)                                                                  aGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGGTCTGGTATCTGATGACCTTTTTGCCCTGGCa                                                                                <  2:1289428/150‑1 (MQ=255)                                                                      gTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGctc                                                                            >  1:1087486/1‑150 (MQ=255)                                                                           gAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTc                                                                       <  1:93280/150‑1 (MQ=255)                                                                                gTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtgg                                                                  >  1:953538/1‑150 (MQ=255)                                                                                   gCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggtgg                                                               >  2:114157/1‑150 (MQ=255)                                                                                   gCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggtgg                                                               >  1:669892/1‑150 (MQ=255)                                                                                    ccTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTATTTGCCCTGGCAGCTCTACTCGCTggtggt                                                              >  1:1356610/1‑150 (MQ=255)                                                                                      tGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGa                                                            >  1:776567/1‑150 (MQ=255)                                                                                          gATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCt                                                        <  2:510815/150‑1 (MQ=255)                                                                                          gATGAGTAATCCGTGGCATCTACTTGCTGTCAGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCt                                                        <  1:1385744/150‑1 (MQ=255)                                                                                            tGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGAt                                                           <  2:206344/145‑1 (MQ=255)                                                                                            tGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGAt                                                           >  1:206344/1‑145 (MQ=255)                                                                                               gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGa                                                   >  1:787611/1‑150 (MQ=255)                                                                                               gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGa                                                   >  1:115409/1‑150 (MQ=255)                                                                                                 aaTCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATc                                                 <  1:114157/150‑1 (MQ=255)                                                                                                   tCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTg                                               <  2:865042/150‑1 (MQ=255)                                                                                                   tCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTg                                               <  1:567089/150‑1 (MQ=255)                                                                                                           aTCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCg                                       <  1:1382370/150‑1 (MQ=255)                                                                                                              tACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCt                                    >  1:1144659/1‑150 (MQ=255)                                                                                                                  tGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCt                                >  1:274089/1‑150 (MQ=255)                                                                                                                    cTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCttt                              >  1:162978/1‑150 (MQ=255)                                                                                                                      gTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGt                            <  1:958991/150‑1 (MQ=255)                                                                                                                      gTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGt                            >  1:350698/1‑150 (MQ=255)                                                                                                                                gAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTg                                                                                        <  1:794575/80‑1 (MQ=255)                                                                                                                                gAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTg                                                                                        >  2:794575/1‑80 (MQ=255)                                                                                                                                  aGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCg                >  2:156866/1‑150 (MQ=255)                                                                                                                                     ggATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGaaa             <  2:274089/150‑1 (MQ=255)                                                                                                                                      gATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAg            <  2:259525/150‑1 (MQ=255)                                                                                                                                            gCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATgg      >  2:433100/1‑150 (MQ=255)                                                                                                                                             gcccaTCGTCCCAGGAACGATCGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATgg      >  1:555406/5‑150 (MQ=255)                                                                                                                                             cccGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATggg     <  2:1144659/150‑1 (MQ=255)                                                                                                                                             cccGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTAATCAAACGGCGAAAGACATggg     >  1:454955/1‑150 (MQ=255)                                                                                                                                              cccaTCGTCCCAGGAACGATCGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATggg     >  2:363304/4‑150 (MQ=255)                                                                                                                                                gATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTTTCATCAAACGGCGAAAGACATGGgtgt  >  1:413306/1‑150 (MQ=255)                                                                                                                                                gATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGgtgt  >  1:188198/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                 TTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGGTGT  >  NC_000913/436547‑436839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑