Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	436,699	T→A	7.7%	P37P (CCT→CCA)	pgpA →	phosphatidylglycerophosphatase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	436,699	0	T	A	7.7%	198.4 / 5.9	65	P37P (CCT→CCA)	pgpA	phosphatidylglycerophosphatase A
Reads supporting (aligned to +/- strand):  ref base T (34/26);  new base A (2/3);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

TTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGGTGT  >  NC_000913/436550‑436839                                                                                                                                                      |                                                                                                                                              ttCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCt                                                                                                                                               >  1:623045/1‑150 (MQ=255)  tCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTg                                                                                                                                              <  2:949433/150‑1 (MQ=255)    gTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTggg                                                                                                                                            >  1:641665/1‑150 (MQ=255)     tGACGGCGAACCTGTTACATTAGACTGGAAAGGAAATGACCATTTTGCCACGCCATAAAGATTTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGAGGCATCTACTTGCTGTCGGATTCGGAACTGGATTAAGGCCGATCGTTCCTCGGa                                                                                                                                           >  2:789979/1‑150 (MQ=255)             aaCCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCC‑ATCGTCCCAGGAACGAT‑cgggc                                                                                                                                 <  1:953/150‑3 (MQ=255)              aCCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCt                                                                                                                                 <  2:1365614/150‑1 (MQ=255)              aCCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCC‑ATCGTCCCAGGAACGATCGGGCt                                                                                                                                 <  2:858171/150‑1 (MQ=255)               ccTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCC‑ATCGTCCCAGGAACGATCGGGCtt                                                                                                                                <  1:650620/150‑2 (MQ=255)                  gTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCt                                                                                                                             <  2:670942/150‑1 (MQ=255)                    tACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTgg                                                                                                                           >  2:723645/1‑150 (MQ=255)                     aCATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGc                                                                                                                          >  2:567089/1‑150 (MQ=255)                       aTTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAg                                                                                                                        <  2:14317/150‑1 (MQ=255)                       aTTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAg                                                                                                                        <  2:1426574/150‑1 (MQ=255)                          aGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGa                                                                                                                     >  1:205639/1‑150 (MQ=255)                             cTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTc                                                                                                                  >  1:1325229/1‑150 (MQ=255)                                     gATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑g                                                                                                                                     >  1:1012577/1‑123 (MQ=255)                                     gATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑g                                                                                                                                     <  2:1012577/123‑1 (MQ=255)                                     gATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTgg                                                                                                          <  2:223072/150‑1 (MQ=255)                                         tGACCTTTTTGCGACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGc                                                                                                                       <  1:955562/133‑1 (MQ=255)                                         tGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGc                                                                                                                       >  2:955562/1‑133 (MQ=255)                                         tGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGt                                                                                                               <  1:659949/141‑1 (MQ=255)                                         tGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGt                                                                                                               >  2:659949/1‑141 (MQ=255)                                               ttttGCCACGCCATAATGATGTCGCGAAATGTCGCCTGAAGATGAGTAATCGGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCGCGTTCGTTCCTGGGTCGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCtgatga                                                                                                <  2:991045/150‑1 (MQ=255)                                                 ttGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGAcc                                                                                              >  2:958991/1‑150 (MQ=255)                                                    ccACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCttt                                                                                           >  1:510815/1‑150 (MQ=255)                                                     cACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCtttt                                                                                          >  2:1396252/1‑150 (MQ=255)                                                        gCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGc                                                                                       <  1:723645/150‑1 (MQ=255)                                                           aTAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCt                                                                                    >  2:461715/1‑150 (MQ=255)                                                           aTAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCt                                                                                    >  1:259525/1‑150 (MQ=255)                                                              aaGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGc                                                                                 >  1:650273/1‑150 (MQ=255)                                                              aaGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGGCGGATTCGGAAGTGGATTAAGCCCGAGCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGc                                                                                 <  1:1252892/150‑1 (MQ=255)                                                               aGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGGTCTGGTATCTGATGACCTTTTTGCCCTGGCa                                                                                <  2:1289428/150‑1 (MQ=255)                                                                   gTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGctc                                                                            >  1:1087486/1‑150 (MQ=255)                                                                        gAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTc                                                                       <  1:93280/150‑1 (MQ=255)                                                                             gTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtgg                                                                  >  1:953538/1‑150 (MQ=255)                                                                                gCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggtgg                                                               >  2:114157/1‑150 (MQ=255)                                                                                gCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCtggtgg                                                               >  1:669892/1‑150 (MQ=255)                                                                                 ccTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTATTTGCCCTGGCAGCTCTACTCGCTggtggt                                                              >  1:1356610/1‑150 (MQ=255)                                                                                   tGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGa                                                            >  1:776567/1‑150 (MQ=255)                                                                                       gATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCt                                                        <  2:510815/150‑1 (MQ=255)                                                                                       gATGAGTAATCCGTGGCATCTACTTGCTGTCAGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCt                                                        <  1:1385744/150‑1 (MQ=255)                                                                                         tGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGAt                                                           >  1:206344/1‑145 (MQ=255)                                                                                         tGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGAt                                                           <  2:206344/145‑1 (MQ=255)                                                                                            gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGa                                                   >  1:115409/1‑150 (MQ=255)                                                                                            gTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGa                                                   >  1:787611/1‑150 (MQ=255)                                                                                              aaTCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATc                                                 <  1:114157/150‑1 (MQ=255)                                                                                                tCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTg                                               <  1:567089/150‑1 (MQ=255)                                                                                                tCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTg                                               <  2:865042/150‑1 (MQ=255)                                                                                                        aTCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCg                                       <  1:1382370/150‑1 (MQ=255)                                                                                                           tACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCt                                    >  1:1144659/1‑150 (MQ=255)                                                                                                               tGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCt                                >  1:274089/1‑150 (MQ=255)                                                                                                                 cTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCttt                              >  1:162978/1‑150 (MQ=255)                                                                                                                   gTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGt                            <  1:958991/150‑1 (MQ=255)                                                                                                                   gTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGt                            >  1:350698/1‑150 (MQ=255)                                                                                                                             gAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTg                                                                                        >  2:794575/1‑80 (MQ=255)                                                                                                                             gAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTg                                                                                        <  1:794575/80‑1 (MQ=255)                                                                                                                               aGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCg                >  2:156866/1‑150 (MQ=255)                                                                                                                                  ggATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGaaa             <  2:274089/150‑1 (MQ=255)                                                                                                                                   gATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAg            <  2:259525/150‑1 (MQ=255)                                                                                                                                         gCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATgg      >  2:433100/1‑150 (MQ=255)                                                                                                                                          gcccaTCGTCCCAGGAACGATCGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATgg      >  1:555406/5‑150 (MQ=255)                                                                                                                                          cccGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATggg     <  2:1144659/150‑1 (MQ=255)                                                                                                                                          cccGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTAATCAAACGGCGAAAGACATggg     >  1:454955/1‑150 (MQ=255)                                                                                                                                           cccaTCGTCCCAGGAACGATCGGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATggg     >  2:363304/4‑150 (MQ=255)                                                                                                                                             gATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTTTCATCAAACGGCGAAAGACATGGgtgt  >  1:413306/1‑150 (MQ=255)                                                                                                                                             gATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGgtgt  >  1:188198/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                              TTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGAT‑GGGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTACTCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAAGACATGGGTGT  >  NC_000913/436550‑436839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑