| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,531,098 | C→A | 5.2% | C185F (TGT→TTT) | sgcX ← | KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,531,098 | 0 | C | A | 5.2% | 264.2 / 3.2 | 77 | C185F (TGT→TTT) | sgcX | KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain |
| Reads supporting (aligned to +/- strand): ref base C (35/38); new base A (2/2); total (37/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCACCAATATCAATCCACAGTTT > NC_000913/4530950‑4531246 | aaTACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCATTACa < 1:1179404/150‑1 (MQ=255) ccAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑Ct > 1:192051/1‑150 (MQ=255) ccAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑Ct > 1:161839/1‑150 (MQ=255) ccAATCGCCAGGTCAGGTTTTACACGGAGTAATTCGGGAACAATGCCGCGGATATTAAATTCTTCCTGTAACGAAGGCACCAGATAAACGGCGATATCAAGCTGCATAGTAGTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGG‑Ct < 2:337983/150‑1 (MQ=255) cAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTa > 2:333591/1‑150 (MQ=255) aaTCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAg < 2:1051834/150‑1 (MQ=255) tCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGAc < 1:207579/150‑1 (MQ=255) gCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGa > 1:1236928/1‑150 (MQ=255) aggtTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAg < 1:743859/150‑1 (MQ=255) tACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCttt > 1:120777/1‑150 (MQ=255) cGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTg > 1:905311/1‑150 (MQ=255) cGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTg > 1:495768/1‑150 (MQ=255) ggCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGATCATGGCATTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGc < 2:227713/150‑1 (MQ=255) gCGTAATACGGTAACAATGCCGCGGATATTAAATTCTTCCTGTACCTAAGCTACCAGATAAACGGCGATATCAAGCTACATAGTACTGATAGCATCAGCTACACCGAGCAGGGCAGTACATC‑CTAGACGATCGTCTAGTGCTTTACTGca < 2:67296/150‑1 (MQ=255) gCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGca < 1:19360/150‑1 (MQ=255) gTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTAAAGCACTAGACGATCGTCTAG‑GCTGTACTGccct < 1:858216/150‑4 (MQ=38) tAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTAAAGCACTAGACGATCGTCTAG‑GCTGTACTGccctg < 1:1102511/150‑5 (MQ=38) aaTACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACAcc > 2:850837/1‑150 (MQ=255) ggAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCg > 1:1403402/1‑150 (MQ=255) ggAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCg < 1:473915/150‑1 (MQ=255) aCAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGttt < 2:161839/150‑1 (MQ=255) gCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAg < 2:446000/150‑1 (MQ=255) tatTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTgcg < 2:750899/150‑1 (MQ=255) tAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTgcggcg > 2:968755/1‑150 (MQ=255) aaaTTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGcggcgg < 2:1000950/150‑1 (MQ=255) aaTTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCggg < 2:92497/150‑1 (MQ=255) aaTTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCggg > 2:206049/1‑150 (MQ=255) aaTTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCggg < 1:531764/150‑1 (MQ=255) aTTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGt < 2:391790/150‑1 (MQ=255) ttcCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTtata < 2:58676/150‑1 (MQ=255) ttcCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTtata > 2:778920/1‑150 (MQ=255) tcCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATAc > 1:681354/1‑150 (MQ=255) ccTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACa > 1:1024313/1‑150 (MQ=255) cTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACGCCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACa > 1:267921/1‑149 (MQ=255) cTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACag > 2:911580/1‑150 (MQ=255) cTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACa < 2:267921/149‑1 (MQ=255) tGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAg > 1:537450/1‑128 (MQ=255) tGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAg < 2:537450/128‑1 (MQ=255) gTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAg > 1:1113139/1‑85 (MQ=255) gTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAg < 2:1113139/85‑1 (MQ=255) tACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGt < 1:1081484/150‑1 (MQ=255) aCCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTg > 2:1282464/1‑150 (MQ=255) ccGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGa < 2:120777/150‑1 (MQ=255) cGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGAc < 2:215727/150‑1 (MQ=255) aGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAgg < 1:1077789/150‑1 (MQ=255) aGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAgg < 1:870143/150‑1 (MQ=255) aGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAgg > 1:596639/1‑150 (MQ=255) ccaccaGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCg < 1:333591/150‑1 (MQ=255) caccaGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGt > 1:847805/1‑150 (MQ=255) tAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGAcc > 1:849603/1‑150 (MQ=255) aaCGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTCCACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTg > 1:112196/1‑150 (MQ=255) aaCGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTg < 2:495768/150‑1 (MQ=255) gATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGcc < 2:681354/150‑1 (MQ=255) tatCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACGCCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCa > 1:1145027/1‑150 (MQ=255) tatCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCa > 1:415818/1‑150 (MQ=255) tCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATc < 2:525514/150‑1 (MQ=255) tCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATc < 2:1403402/150‑1 (MQ=255) cTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGtat < 2:415818/150‑1 (MQ=255) cTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACATGAATGCCCATGCGtat < 2:192051/150‑1 (MQ=255) ccATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAg > 1:994397/1‑150 (MQ=255) cATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGc > 1:923/1‑150 (MQ=255) tAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGcgt < 1:206049/150‑1 (MQ=255) aGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGcgtc > 2:1002052/1‑150 (MQ=255) aGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGcgtc < 2:596639/150‑1 (MQ=255) tGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCgtctt > 1:354233/1‑150 (MQ=255) gCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTAAAGCGTCgtctttgtct > 1:660431/1‑150 (MQ=255) tCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGtctttgtctttg < 1:968755/150‑1 (MQ=255) gCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCa > 1:1429505/1‑150 (MQ=255) cTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCAc < 2:994397/150‑1 (MQ=255) ccGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCACCAata > 2:1079816/1‑150 (MQ=255) gAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTTAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCACCAATATc < 2:1236928/150‑1 (MQ=255) gAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCACCAATATc < 1:911580/150‑1 (MQ=255) tgtgCAGTAAAGCACTAGACGATCGTCTAG‑GCTGTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCACCAATATCAATc > 1:659870/4‑150 (MQ=38) gCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCACCAATATCAATCcac > 1:77602/1‑150 (MQ=255) gCAGTAAAGCACTAGACGATCGTCTAG‑GCTGTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCACCAATATCAATCcac > 2:1406206/1‑150 (MQ=39) aCAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCACCAATATCAATCCACAGttt > 1:798709/1‑150 (MQ=255) aCAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCACCAATATCAATCCACAGttt > 1:1285667/1‑150 (MQ=255) | AATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGATAAACGGCGATATCAAGCTCCATAGTACTGATAGCATCCGCTACACCGAGCAGGGCAGTACAGC‑CTAGACGATCGTCTAGTGCTTTACTGCACACCAGATCGTTTGCCAGGAGTTGCGGCGGGTTATACAGAGTGACAGGCGTACCGACCTGAATGCCCATCCGTATAGCGTCGTCTTTGTCTTTGGCACCAATATCAATCCACAGTTT > NC_000913/4530950‑4531246 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |