| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 79,860 | G→T | 7.5% | T335T (ACC→ACA) | leuC ← | 3‑isopropylmalate dehydratase subunit LeuC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 79,860 | 0 | G | T | 7.5% | 172.3 / 4.1 | 53 | T335T (ACC→ACA) | leuC | 3‑isopropylmalate dehydratase subunit LeuC |
| Reads supporting (aligned to +/- strand): ref base G (22/27); new base T (2/2); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01 | |||||||||||
TGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTG > NC_000913/79711‑80006 | tGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCg < 2:191579/150‑1 (MQ=255)tGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCg < 2:1125247/150‑1 (MQ=255) cTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCa > 1:1404645/1‑150 (MQ=255) attttACCGGGCCAGAGCCGCGAACCACCAGTGCCTGCACGCATGGCCCGACTTTTCGCCCTTTGGCTCTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGACACTTCGGTCa > 2:1175192/2‑150 (MQ=255) tACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCgg < 1:89649/150‑1 (MQ=255) ccGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCgg < 2:922429/141‑1 (MQ=255) ccGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCgg > 1:922429/1‑141 (MQ=255) ccAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATAccc < 2:1085492/150‑1 (MQ=255) cAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCg < 1:1225931/150‑1 (MQ=255) ccGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTc > 1:612834/1‑150 (MQ=255) ccGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTc < 2:531252/150‑1 (MQ=255) cGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCa < 1:787185/150‑1 (MQ=255) cGGGAACCACCAGTGCCTGCACGCCGGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGGCAGCGGAATACCCGGTTTCa < 2:888831/150‑1 (MQ=255) accaccAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGcccc < 1:934178/150‑1 (MQ=255) accaccAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGcccc > 1:144288/1‑150 (MQ=255) ccaccaGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCa > 2:1156736/1‑150 (MQ=255) ccaccaGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCa < 2:553985/150‑1 (MQ=255) accaGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCata > 1:1036256/1‑150 (MQ=255) accaGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCata > 1:1390847/1‑150 (MQ=255) cTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAg > 2:1246433/1‑150 (MQ=255) tGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAgc < 1:1316999/150‑1 (MQ=255) ccTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCtttt < 2:359908/150‑1 (MQ=255) ccTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCtttt < 2:354717/150‑1 (MQ=255) gcgACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGcc < 1:861406/150‑1 (MQ=255) aCTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAc > 1:149298/1‑150 (MQ=255) aCTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCAATATAGGCAAGCGCTTTTTCTGCCGAc < 2:797497/150‑1 (MQ=255) cTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCCTATAGGCCAGCGCTTTTTCTGCCGAcg > 1:43633/1‑150 (MQ=255) cGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAg > 1:939036/1‑123 (MQ=255) cGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAg < 2:939036/123‑1 (MQ=255) ccTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCa > 2:889590/1‑150 (MQ=255) ccTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCa > 1:1196767/1‑150 (MQ=255) tctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCgg < 1:1336563/150‑1 (MQ=255) ccGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAg < 1:57410/150‑1 (MQ=255) cgcgcgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTGAACCGGATCGGCAAACGAAGc > 1:944817/1‑150 (MQ=255) cgcgcgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGc > 2:1085874/1‑150 (MQ=255) gcgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCgg < 2:257129/150‑1 (MQ=255) aTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGGGGAATACCCGGTTTGAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAGCGGATCGGCAAACGAAGGCGGATCggg < 2:1067776/150‑1 (MQ=255) aTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCCTATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCggg < 2:43633/150‑1 (MQ=255) tCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGa < 1:1358785/150‑1 (MQ=255) cTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGACCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAAAGAAGCCGGATCGGGaa > 1:513861/1‑150 (MQ=255) cAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAatat < 1:1156736/150‑1 (MQ=255) cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCg > 1:256080/1‑150 (MQ=255) tGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACgg < 1:127738/150‑1 (MQ=255) ggTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGa > 2:116439/1‑150 (MQ=255) ggTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGa > 2:111261/1‑150 (MQ=255) tAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCgg < 2:144288/150‑1 (MQ=255) aaaCACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTGAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCggg < 2:944817/150‑1 (MQ=255) aaCACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGa > 2:404914/1‑150 (MQ=255) acTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATATGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGTTCGTGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTg < 1:1175192/150‑1 (MQ=255) gTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGcc < 1:116439/150‑1 (MQ=255) gTCGATAGCCACTTCTGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGcc < 1:111261/150‑1 (MQ=255) gTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGcc > 2:757725/1‑150 (MQ=255) tCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCGGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGccc < 2:696707/150‑1 (MQ=255) gATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCa > 1:1270352/1‑150 (MQ=255) gATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCa > 1:1265173/1‑150 (MQ=255) aCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGAcc < 2:1196767/150‑1 (MQ=255) ttCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTg > 1:759494/1‑150 (MQ=255) | TGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCCGGATCGGGAATATTGTCGTTCACGGAAATCACCTGGCCGGGATTGGTGCCCCAGGTGACCTG > NC_000913/79711‑80006 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |