Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 747,902 C→G 8.9% intergenic (‑133/+19) abrB ← / ← ybgO putative regulator/putative fimbrial protein YbgO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913747,9020CG 8.9% 257.8 / 13.3 79intergenic (‑133/+19)abrB/ybgOputative regulator/putative fimbrial protein YbgO
Reads supporting (aligned to +/- strand):  ref base C (31/41);  new base G (3/4);  total (34/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

GCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGGCG  >  NC_000913/747757‑748050
                                                                                                                                                 |                                                                                                                                                    
gCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAg                                                                                                                                                  >  1:657133/1‑150 (MQ=255)
  aaaaCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAggg                                                                                                                                                >  2:869399/1‑150 (MQ=255)
  aaaaCTGGCATCCTTCGCTACCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAggg                                                                                                                                                <  2:1288620/150‑1 (MQ=255)
   aaaCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGGAAAGGGc                                                                                                                                               <  2:380100/150‑1 (MQ=255)
     aCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGGAAAGGGcat                                                                                                                                             <  1:315251/150‑1 (MQ=255)
     aCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGcat                                                                                                                                             <  2:372224/150‑1 (MQ=255)
     aCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGcat                                                                                                                                             <  2:367233/150‑1 (MQ=255)
          cATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATgg                                                                                                                                        <  1:129838/150‑1 (MQ=255)
           aTCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGt                                                                                                                                       <  2:408077/150‑1 (MQ=255)
            tCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTa                                                                                                                                      <  2:805791/150‑1 (MQ=255)
            tCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTa                                                                                                                                      <  2:663488/150‑1 (MQ=255)
             ccTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGGAAAGGGcatctctttca                                                                                                                                     <  2:1153736/150‑8 (MQ=255)
                tCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATc                                                                                                                                  <  2:101038/150‑1 (MQ=255)
                  gCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAg                                                                                                                                <  1:1116597/150‑1 (MQ=255)
                   cTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGt                                                                                                                               <  1:211674/150‑1 (MQ=255)
                   cTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGt                                                                                                                               >  1:1243420/1‑150 (MQ=255)
                   cTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGt                                                                                                                               <  2:1052830/150‑1 (MQ=255)
                        tCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAg                                                                                                                          >  1:709444/1‑150 (MQ=255)
                        tCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAg                                                                                                                          >  2:1151432/1‑150 (MQ=255)
                              ttttCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATc                                                                                                                    >  1:410160/1‑150 (MQ=255)
                                 tctcCTTGGTGTATCGATACAGCATAAAGACAGCTGGGCCAGGAAAAAATCACAGCAATCAAGGAAAAGGGGAAACTCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTa                                                                                                                 <  2:321212/150‑1 (MQ=255)
                                   tctTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaa                                                                                                               <  2:156848/150‑1 (MQ=255)
                                    ctTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaaa                                                                                                              <  1:1151432/150‑1 (MQ=255)
                                        ggTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGca                                                                                                          >  2:997959/1‑150 (MQ=255)
                                        ggTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGca                                                                                                          >  1:977304/1‑150 (MQ=255)
                                           gtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGtt                                                                                                                              >  2:1010356/1‑127 (MQ=255)
                                           gtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGtt                                                                                                                              <  1:1010356/127‑1 (MQ=255)
                                           gtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGcacca                                                                                                       >  1:340408/1‑150 (MQ=255)
                                             aTCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACt                                                                                                     >  1:288803/1‑150 (MQ=255)
                                              tCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTg                                                                                                    <  2:124734/150‑1 (MQ=255)
                                                  tACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATc                                                                                                <  2:168208/150‑1 (MQ=255)
                                                      gCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAg                                                                                            >  1:89774/1‑150 (MQ=255)
                                                      gCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAg                                                                                            <  2:340408/150‑1 (MQ=255)
                                                      gCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCAATGTATCGAAg                                                                                            >  2:513713/1‑150 (MQ=255)
                                                       cATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAgg                                                                                           >  1:42157/1‑150 (MQ=255)
                                                         tAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGGAAAggg                                                                                                                                                <  2:1345257/95‑1 (MQ=255)
                                                         tAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGGAAAggg                                                                                                                                                >  1:1345257/1‑95 (MQ=255)
                                                          aaagaaagCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGc                                                                                        >  2:842636/1‑150 (MQ=255)
                                                           aagaaagCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGcc                                                                                       <  2:1391907/150‑1 (MQ=255)
                                                                  cTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCg                                                                                <  1:891841/150‑1 (MQ=255)
                                                                        cAGGAAAAACTCACTGCAATCAAGGCAAAGCGGAATATCTGCAATTTTCGGCAAGCGAGGCCCTTTCCGGCGGCAAAGGGCATCTTGGTAACTCCGTTATCGTTCATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgggg                                                                          <  1:1360232/150‑1 (MQ=255)
                                                                          ggAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaa                                                                                                               <  2:625619/111‑1 (MQ=255)
                                                                          ggAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaa                                                                                                               >  1:625619/1‑111 (MQ=255)
                                                                          ggAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGt                                                                        >  1:620561/1‑150 (MQ=255)
                                                                           gAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTg                                                                       <  1:667012/150‑1 (MQ=255)
                                                                               aaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTg                                                                   <  2:572518/150‑1 (MQ=255)
                                                                                gaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTgg                                                                  >  2:465548/2‑150 (MQ=255)
                                                                                   aaCAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCA‑CACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATg                                                              <  2:745611/150‑1 (MQ=255)
                                                                                     cAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATgg                                                             >  2:727743/1‑150 (MQ=255)
                                                                                       gCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTATAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGAc                                                           <  2:479723/150‑1 (MQ=255)
                                                                                       gCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGAc                                                           >  1:1187111/1‑150 (MQ=255)
                                                                                         aaTCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGa                                                         >  1:1031126/1‑150 (MQ=255)
                                                                                               ggAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTc                                                   <  2:953238/150‑1 (MQ=255)
                                                                                                      gggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAg                                                      >  2:995457/1‑140 (MQ=255)
                                                                                                      gggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAg                                                      <  1:995457/140‑1 (MQ=255)
                                                                                                          aaaTCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAgg                                        <  1:465548/150‑1 (MQ=255)
                                                                                                            aTCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCGCGATGGGGGTGCCTGGAAGGGACGAAAGTTCAGCCGTATAGGtt                                      >  2:243796/1‑150 (MQ=255)
                                                                                                             tCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGttt                                     >  2:1072851/1‑150 (MQ=255)
                                                                                                               aGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGttttt                                   <  2:173984/150‑1 (MQ=255)
                                                                                                                gCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGtttttt                                  <  2:89774/150‑1 (MQ=255)
                                                                                                                gCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGtttttt                                  >  2:829349/1‑150 (MQ=255)
                                                                                                                 cAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTc                                 <  1:869399/150‑1 (MQ=255)
                                                                                                                   aTTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGt                               <  1:70367/150‑1 (MQ=255)
                                                                                                                   aTTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGt                               <  2:791193/150‑1 (MQ=255)
                                                                                                                     tttCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCGGTATAGGTTTTTTCGTAg                             <  1:997959/150‑1 (MQ=255)
                                                                                                                     tttCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAg                             <  1:795174/150‑1 (MQ=255)
                                                                                                                        cTGAACGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAACTCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGGTCAGCCGTATAGGTTTTTTCGTAGAcc                          <  2:1109053/150‑1 (MQ=255)
                                                                                                                        cTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGAcc                          <  2:657133/150‑1 (MQ=255)
                                                                                                                          gAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCaa                        >  2:110940/1‑150 (MQ=255)
                                                                                                                          gAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCaa                        >  1:1372860/1‑150 (MQ=255)
                                                                                                                               tgaTGCCCTTTGCCGCCGGAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGt                   >  2:1269946/2‑150 (MQ=255)
                                                                                                                                taTGCCCTTTCCGGCGGCAAAGGGCATCATTGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTc                  <  1:599440/149‑1 (MQ=255)
                                                                                                                                  tGCCCTTTGCCGCCGGAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCAc                >  1:1403092/1‑150 (MQ=255)
                                                                                                                                   gCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACt               >  2:321205/1‑150 (MQ=255)
                                                                                                                                      cTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGt            <  2:709444/150‑1 (MQ=255)
                                                                                                                                          ccGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACAt                     <  2:527281/137‑1 (MQ=255)
                                                                                                                                          ccGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACAt                     >  1:527281/1‑137 (MQ=255)
                                                                                                                                           cggcggCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAAt       >  1:547227/1‑150 (MQ=255)
                                                                                                                                           cggcggCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAAt       >  1:380735/1‑150 (MQ=255)
                                                                                                                                            ggcggcAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATc      >  1:1130281/1‑150 (MQ=255)
                                                                                                                                               ggcAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGGc   <  2:150651/150‑1 (MQ=255)
                                                                                                                                                gcAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGGCg  <  2:177708/150‑1 (MQ=255)
                                                                                                                                                gcAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGGCg  <  1:727743/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGGCG  >  NC_000913/747757‑748050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑