Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 769,798 T→G 5.3% N607K (AAT→AAG)  mngB → alpha‑mannosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913769,7980TG 5.3% 241.1 / 4.9 76N607K (AAT→AAG) mngBalpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base T (31/41);  new base G (2/2);  total (33/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

CTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTGGA  >  NC_000913/769651‑769946
                                                                                                                                                   |                                                                                                                                                    
cTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCt                                                                                                                                                    <  2:628463/150‑1 (MQ=255)
     agagCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATa                                                                                                                                               <  1:769793/150‑1 (MQ=255)
          aGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGTTATCAAGATTGATATCCac                                                                                                                                          <  2:31245/150‑2 (MQ=255)
          aGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAg                                                                                                                                          >  1:32327/1‑150 (MQ=255)
           gggCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAAt                                                                                                                                                      >  2:1203250/1‑137 (MQ=255)
           gggCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAAt                                                                                                                                                      <  1:1203250/137‑1 (MQ=255)
           gggCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAgg                                                                                                                                         >  1:607816/1‑150 (MQ=255)
            ggCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGc                                                                                                                                        <  2:1219981/150‑1 (MQ=255)
             gCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCt                                                                                                                                       >  1:664152/1‑150 (MQ=255)
             gCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCt                                                                                                                                       >  1:331860/1‑150 (MQ=255)
                gTTATCCGCTATCACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACGGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGAGATCAATCTTGATAACCAGGCGGAc                                                                                                                                    >  2:499115/1‑150 (MQ=255)
                   aTCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGat                                                                                                                                 >  1:1039770/1‑150 (MQ=255)
                     ccGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATACCAATCTTGATAACCAGGCTGACGatca                                                                                                                               <  1:1423571/150‑1 (MQ=255)
                         aaTGACATGGCAGTGCCGCTCATTTTGTATGACTGCAGCGCCCGGCAATCCCCTGTCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCCCATCAGCATGCGTATTGATGTGGATAGCAATCTTGATAACCAGGCTGACGATCATCGc                                                                                                                           <  2:1002886/149‑1 (MQ=255)
                             aCATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTc                                                                                                                       >  1:666041/1‑150 (MQ=255)
                                 ggCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCgtgt                                                                                                                   <  1:379037/150‑1 (MQ=255)
                                 ggCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCgtgt                                                                                                                   <  2:607816/150‑1 (MQ=255)
                                  gCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTc                                                                                                                  >  2:607330/1‑150 (MQ=255)
                                    aGTGGCGCTCATTTTGTGAGAACGCAGCGCCCGGCAATCCACTTGCATAGTAGTGGTGGTTTTGGTTGTCACTCTTAGTCATAACATCAGGCGTTTTGATGTGTATATCAATATTGATAACCTGGCTGACTATCATCGCCTTCGTGTCCt                                                                                                                <  2:1167469/150‑1 (MQ=255)
                                    aGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGGGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCt                                                                                                                <  2:233836/150‑1 (MQ=255)
                                     gTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGTCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTg                                                                                                               <  2:920602/150‑1 (MQ=255)
                                           cTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCt                                                                                                         <  2:1325980/150‑1 (MQ=255)
                                                tttGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACAcc                                                                                                    <  1:1387782/150‑1 (MQ=255)
                                                 ttGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCt                                                                                                   <  2:1101062/150‑1 (MQ=255)
                                                    tCAGAACGCAGCTCCCGGCAATCCACTGGGAGAGTAGGGGTTGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCTTATTGATGTTGATATAATTGTTGATAACCAGGCTGAAGATGATCGTCTTCGTGTCTTGGTCTATACAACtttt                                                                                                <  2:1214885/150‑1 (MQ=255)
                                                    tCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCtttt                                                                                                <  2:331860/150‑1 (MQ=255)
                                                    tCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGATGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCtttt                                                                                                <  2:1341217/150‑1 (MQ=255)
                                                    gcAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGGGGTGGTGGTTGTCACTCTTAGTCATAACAGCAGGCGGAGTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCtttt                                                                                                <  2:934911/149‑1 (MQ=255)
                                                     cAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTa                                                                                               <  1:1026438/150‑1 (MQ=255)
                                                       caaCGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAac                                                                                             >  1:1012995/2‑150 (MQ=255)
                                                          cGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACAcc                                                                                          <  2:664152/150‑1 (MQ=255)
                                                            cAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGa                                                                                        <  2:819320/150‑1 (MQ=255)
                                                             aGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGAc                                                                                       <  2:666041/150‑1 (MQ=255)
                                                              gcgcCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAACAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACa                                                                                      <  1:986558/150‑1 (MQ=255)
                                                              gcgcCCGGCAATCCACTGGCAGAGTAGGGGTGGTGGTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACa                                                                                      <  2:593917/150‑1 (MQ=255)
                                                              gcgcCCGGCAATCCACTGGCAGAGTAGGGGGGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACa                                                                                      <  2:1305831/150‑1 (MQ=255)
                                                                   cGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGtt                                                                                 >  2:580144/1‑150 (MQ=255)
                                                                          ccACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCGCTACACCTTTTATCACCGACAGTGTTCTCGCAg                                                                          >  1:945450/1‑150 (MQ=255)
                                                                              tGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATAc                                                                      >  1:1281836/1‑150 (MQ=255)
                                                                               ggCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACg                                                                     >  1:1088587/1‑150 (MQ=255)
                                                                               ggCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACg                                                                     <  2:1082970/150‑1 (MQ=255)
                                                                               ggCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACg                                                                     >  1:1421089/1‑150 (MQ=255)
                                                                               ggCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACg                                                                     >  2:463152/1‑150 (MQ=255)
                                                                               ggCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACAACTTTTAACACCGACAGTGTTCTGGCAGATACg                                                                     >  2:881035/1‑150 (MQ=255)
                                                                                       aGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTgg                                                             <  2:1088587/150‑1 (MQ=255)
                                                                                        ggggTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGt                                                            >  2:1050463/1‑150 (MQ=255)
                                                                                          tgtggtgTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGtta                                                          >  1:1086789/2‑149 (MQ=255)
                                                                                           gtggtgTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCg                                                         >  2:483803/1‑150 (MQ=255)
                                                                                            tggtgTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGc                                                        <  1:470141/150‑1 (MQ=255)
                                                                                              gtgtTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTa                                                      <  1:1282553/150‑1 (MQ=255)
                                                                                                gttggttgTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAAc                                                    >  1:1348200/1‑150 (MQ=255)
                                                                                                        tCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCg                                            >  2:1281967/1‑150 (MQ=255)
                                                                                                             ctTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAAc                                       <  1:126452/150‑1 (MQ=255)
                                                                                                             ctTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAAc                                       <  2:874150/150‑1 (MQ=255)
                                                                                                             ctTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGAGCATCGCCTTCGTGTCCTGGTCCCTTCACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAAc                                       <  1:1316202/150‑1 (MQ=255)
                                                                                                              ttAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACg                                      <  1:607330/150‑1 (MQ=255)
                                                                                                                aGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGATGACGATCATCGCCTTCGTGTCCTg                                                                                                               >  1:729121/1‑75 (MQ=255)
                                                                                                                aGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGATGACGATCATCGCCTTCGTGTCCTg                                                                                                               <  2:729121/75‑1 (MQ=255)
                                                                                                                    aTAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTg                                <  1:1281967/150‑1 (MQ=255)
                                                                                                                     tAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCCGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGc                               <  2:1299062/150‑1 (MQ=255)
                                                                                                                     tAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGc                               <  2:1039770/150‑1 (MQ=255)
                                                                                                                     tAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGc                               <  1:719179/150‑1 (MQ=255)
                                                                                                                            agGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGaac                        <  2:972953/150‑1 (MQ=255)
                                                                                                                                  aTTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTgg                  <  1:106624/150‑1 (MQ=255)
                                                                                                                                   ttGATGTGGATATCAATCTTGATAACCAGGCTGACGCTCATCGCCATCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgc                 >  1:985517/1‑150 (MQ=255)
                                                                                                                                   ttGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgc                 <  2:379928/150‑1 (MQ=255)
                                                                                                                                   ttGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgc                 >  2:1194233/1‑150 (MQ=255)
                                                                                                                                   ttGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTCGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgc                 <  2:291765/150‑1 (MQ=255)
                                                                                                                                    cagcctgGTTATCAAGATTGATATCCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgca                <  2:480887/145‑1 (MQ=255)
                                                                                                                                    cagcctgGTTATCAAGATTGATATCCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgca                >  1:480887/6‑150 (MQ=255)
                                                                                                                                      gcctgGTTATCAAGATTGATATCCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgcagc              >  1:984496/4‑150 (MQ=255)
                                                                                                                                         tgGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCaag           >  1:60853/1‑150 (MQ=255)
                                                                                                                                          ggATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCaaga          >  1:1368877/1‑150 (MQ=255)
                                                                                                                                          ggATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCaaga          >  2:701485/1‑150 (MQ=255)
                                                                                                                                           gATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCaagaa         >  2:614137/1‑150 (MQ=255)
                                                                                                                                              atCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGc      >  1:287543/1‑150 (MQ=255)
                                                                                                                                                cAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTg    >  2:1000250/1‑150 (MQ=255)
                                                                                                                                                  aTCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTGGa  >  1:439409/1‑150 (MQ=255)
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CTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTGGA  >  NC_000913/769651‑769946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑