Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 769,806 A→T 6.6% N610I (AAC→ATC)  mngB → alpha‑mannosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913769,8060AT 6.6% 237.1 / 5.1 76N610I (AAC→ATC) mngBalpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base A (32/39);  new base T (2/3);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

AGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTGGAAAGAAGC  >  NC_000913/769661‑769953
                                                                                                                                                 |                                                                                                                                                   
aGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGTTATCAAGATTGATATCCac                                                                                                                                                 <  2:31245/150‑2 (MQ=255)
aGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAg                                                                                                                                                 >  1:32327/1‑150 (MQ=255)
 gggCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAgg                                                                                                                                                >  1:607816/1‑150 (MQ=255)
  ggCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGc                                                                                                                                               <  2:1219981/150‑1 (MQ=255)
   gCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCt                                                                                                                                              >  1:664152/1‑150 (MQ=255)
   gCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCt                                                                                                                                              >  1:331860/1‑150 (MQ=255)
      gTTATCCGCTATCACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACGGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGAGATCAATCTTGATAACCAGGCGGAc                                                                                                                                           >  2:499115/1‑150 (MQ=255)
         aTCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGat                                                                                                                                        >  1:1039770/1‑150 (MQ=255)
           ccGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATACCAATCTTGATAACCAGGCTGACGatca                                                                                                                                      <  1:1423571/150‑1 (MQ=255)
               aaTGACATGGCAGTGCCGCTCATTTTGTATGACTGCAGCGCCCGGCAATCCCCTGTCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCCCATCAGCATGCGTATTGATGTGGATAGCAATCTTGATAACCAGGCTGACGATCATCGc                                                                                                                                  <  2:1002886/149‑1 (MQ=255)
                   aCATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTc                                                                                                                              >  1:666041/1‑150 (MQ=255)
                       ggCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCgtgt                                                                                                                          <  2:607816/150‑1 (MQ=255)
                       ggCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCgtgt                                                                                                                          <  1:379037/150‑1 (MQ=255)
                        gCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTc                                                                                                                         >  2:607330/1‑150 (MQ=255)
                          aGTGGCGCTCATTTTGTGAGAACGCAGCGCCCGGCAATCCACTTGCATAGTAGTGGTGGTTTTGGTTGTCACTCTTAGTCATAACATCAGGCGTTTTGATGTGTATATCAATATTGATAACCTGGCTGACTATCATCGCCTTCGTGTCCt                                                                                                                       <  2:1167469/150‑1 (MQ=255)
                          aGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGGGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCt                                                                                                                       <  2:233836/150‑1 (MQ=255)
                           gTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGTCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTg                                                                                                                      <  2:920602/150‑1 (MQ=255)
                                 cTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCt                                                                                                                <  2:1325980/150‑1 (MQ=255)
                                      tttGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACAcc                                                                                                           <  1:1387782/150‑1 (MQ=255)
                                       ttGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCt                                                                                                          <  2:1101062/150‑1 (MQ=255)
                                          tCAGAACGCAGCTCCCGGCAATCCACTGGGAGAGTAGGGGTTGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCTTATTGATGTTGATATAATTGTTGATAACCAGGCTGAAGATGATCGTCTTCGTGTCTTGGTCTATACAACtttt                                                                                                       <  2:1214885/150‑1 (MQ=255)
                                          tCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCtttt                                                                                                       <  2:331860/150‑1 (MQ=255)
                                          tCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGATGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCtttt                                                                                                       <  2:1341217/150‑1 (MQ=255)
                                          gcAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGGGGTGGTGGTTGTCACTCTTAGTCATAACAGCAGGCGGAGTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCtttt                                                                                                       <  2:934911/149‑1 (MQ=255)
                                           cAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTa                                                                                                      <  1:1026438/150‑1 (MQ=255)
                                             caaCGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAac                                                                                                    >  1:1012995/2‑150 (MQ=255)
                                                cGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACAcc                                                                                                 <  2:664152/150‑1 (MQ=255)
                                                  cAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGa                                                                                               <  2:819320/150‑1 (MQ=255)
                                                   aGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGAc                                                                                              <  2:666041/150‑1 (MQ=255)
                                                    gcgcCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAACAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACa                                                                                             <  1:986558/150‑1 (MQ=255)
                                                    gcgcCCGGCAATCCACTGGCAGAGTAGGGGTGGTGGTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACa                                                                                             <  2:593917/150‑1 (MQ=255)
                                                    gcgcCCGGCAATCCACTGGCAGAGTAGGGGGGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACa                                                                                             <  2:1305831/150‑1 (MQ=255)
                                                         cGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGtt                                                                                        >  2:580144/1‑150 (MQ=255)
                                                                ccACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCGCTACACCTTTTATCACCGACAGTGTTCTCGCAg                                                                                 >  1:945450/1‑150 (MQ=255)
                                                                    tGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATAc                                                                             >  1:1281836/1‑150 (MQ=255)
                                                                     ggCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACg                                                                            >  2:463152/1‑150 (MQ=255)
                                                                     ggCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACg                                                                            <  2:1082970/150‑1 (MQ=255)
                                                                     ggCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACg                                                                            >  1:1088587/1‑150 (MQ=255)
                                                                     ggCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACg                                                                            >  1:1421089/1‑150 (MQ=255)
                                                                     ggCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACAACTTTTAACACCGACAGTGTTCTGGCAGATACg                                                                            >  2:881035/1‑150 (MQ=255)
                                                                             aGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTgg                                                                    <  2:1088587/150‑1 (MQ=255)
                                                                              ggggTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGt                                                                   >  2:1050463/1‑150 (MQ=255)
                                                                                tgtggtgTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGtta                                                                 >  1:1086789/2‑149 (MQ=255)
                                                                                 gtggtgTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCg                                                                >  2:483803/1‑150 (MQ=255)
                                                                                  tggtgTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGc                                                               <  1:470141/150‑1 (MQ=255)
                                                                                    gtgtTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTa                                                             <  1:1282553/150‑1 (MQ=255)
                                                                                      gttggttgTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAAc                                                           >  1:1348200/1‑150 (MQ=255)
                                                                                              tCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCg                                                   >  2:1281967/1‑150 (MQ=255)
                                                                                                   ctTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAAc                                              <  1:126452/150‑1 (MQ=255)
                                                                                                   ctTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAAc                                              <  2:874150/150‑1 (MQ=255)
                                                                                                   ctTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGAGCATCGCCTTCGTGTCCTGGTCCCTTCACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAAc                                              <  1:1316202/150‑1 (MQ=255)
                                                                                                    ttAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACg                                             <  1:607330/150‑1 (MQ=255)
                                                                                                      aGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGATGACGATCATCGCCTTCGTGTCCTg                                                                                                                      <  2:729121/75‑1 (MQ=255)
                                                                                                      aGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGATGACGATCATCGCCTTCGTGTCCTg                                                                                                                      >  1:729121/1‑75 (MQ=255)
                                                                                                          aTAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTg                                       <  1:1281967/150‑1 (MQ=255)
                                                                                                           tAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCCGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGc                                      <  2:1299062/150‑1 (MQ=255)
                                                                                                           tAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGc                                      <  2:1039770/150‑1 (MQ=255)
                                                                                                           tAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGc                                      <  1:719179/150‑1 (MQ=255)
                                                                                                                  agGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGaac                               <  2:972953/150‑1 (MQ=255)
                                                                                                                        aTTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTgg                         <  1:106624/150‑1 (MQ=255)
                                                                                                                         ttGATGTGGATATCAATCTTGATAACCAGGCTGACGCTCATCGCCATCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgc                        >  1:985517/1‑150 (MQ=255)
                                                                                                                         ttGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgc                        <  2:379928/150‑1 (MQ=255)
                                                                                                                         ttGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgc                        >  2:1194233/1‑150 (MQ=255)
                                                                                                                         ttGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTCGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgc                        <  2:291765/150‑1 (MQ=255)
                                                                                                                          cagcctgGTTATCAAGATTGATATCCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgca                       <  2:480887/145‑1 (MQ=255)
                                                                                                                          cagcctgGTTATCAAGATTGATATCCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgca                       >  1:480887/6‑150 (MQ=255)
                                                                                                                            gcctgGTTATCAAGATTGATATCCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGgcagc                     >  1:984496/4‑150 (MQ=255)
                                                                                                                               tgGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCaag                  >  1:60853/1‑150 (MQ=255)
                                                                                                                                ggATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCaaga                 >  2:701485/1‑150 (MQ=255)
                                                                                                                                ggATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCaaga                 >  1:1368877/1‑150 (MQ=255)
                                                                                                                                 gATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCaagaa                >  2:614137/1‑150 (MQ=255)
                                                                                                                                    atCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGc             >  1:287543/1‑150 (MQ=255)
                                                                                                                                      caagattGATATCCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTg           <  2:984496/145‑1 (MQ=255)
                                                                                                                                      cAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTg           >  2:1000250/1‑150 (MQ=255)
                                                                                                                                        aTCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTGGa         >  1:439409/1‑150 (MQ=255)
                                                                                                                                               tAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTGGAAAGAAgc  >  2:805879/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACGCGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTGGAAAGAAGC  >  NC_000913/769661‑769953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑