Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	774,358	T→A	17.3%	L17Q (CTG→CAG)	ybgE →	cyd operon protein YbgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	774,358	0	T	A	17.3%	118.1 / 18.7	52	L17Q (CTG→CAG)	ybgE	cyd operon protein YbgE
Reads supporting (aligned to +/- strand):  ref base T (22/20);  new base A (2/7);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

TGGGAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGGTGTGATTCACGG  >  NC_000913/774218‑774502                                                                                                                                             |                                                                                                                                                 tGGGAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCGCAGGGGGCGCt                                                                                                                                         <  1:790488/150‑1 (MQ=255)  gggAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCtt                                                                                                                                        <  1:779930/150‑1 (MQ=255)  gggAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCtt                                                                                                                                        >  1:841582/1‑150 (MQ=255)    gAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCGCAGGGGGCGCttgt                                                                                                                                      <  2:461140/150‑3 (MQ=255)    gAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCGCAGGGGGCGCttgt                                                                                                                                      <  1:821398/150‑3 (MQ=255)    gAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTc                                                                                                                                      >  1:1180577/1‑150 (MQ=255)     aaCGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCGCAGGGGGCGCttgtc                                                                                                                                     <  2:1010845/150‑4 (MQ=255)      aCGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCGCAGGGGGCGCttgtcc                                                                                                                                    <  1:231969/150‑5 (MQ=255)       cGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCtt                                                                                                                                   <  2:372666/150‑1 (MQ=255)        gCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCGCAGGGGGCGCttgtccat                                                                                                                                  <  2:1151157/150‑7 (MQ=255)        gCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTc                                                                                                                                  >  2:1212723/1‑150 (MQ=255)         cttcttGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCg                                                                                                                                 <  2:225062/150‑1 (MQ=255)         cttcttGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCg                                                                                                                                 <  2:1277474/150‑1 (MQ=255)                       gTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTg                                                                                                                   <  2:638310/150‑1 (MQ=255)                            gggTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGc                                                                                                              >  2:1055638/1‑150 (MQ=255)                                  tCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGatg                                                                                                        <  2:433288/150‑1 (MQ=255)                                    aCCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGatgta                                                                                                      >  1:794711/1‑150 (MQ=255)                                    aCCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGatgta                                                                                                      >  2:895435/1‑150 (MQ=255)                                                cTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGAcc                                                                                          <  2:51923/150‑1 (MQ=255)                                                 ttGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGAccc                                                                                         <  2:841582/150‑1 (MQ=255)                                                  tGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCa                                                                                        >  2:1013370/1‑150 (MQ=255)                                                  tGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCa                                                                                        >  2:1225042/1‑150 (MQ=255)                                                          tCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGttt                                                                                <  1:1183698/150‑1 (MQ=255)                                                          tCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGACGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGttt                                                                                <  1:390608/150‑1 (MQ=255)                                                            gAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTg                                                                              >  1:129275/1‑150 (MQ=255)                                                                  ggCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGa                                                                        >  1:1291682/1‑150 (MQ=255)                                                                  ggCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGa                                                                        >  2:619202/1‑150 (MQ=255)                                                                    cAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAg                                                                      >  1:94827/1‑150 (MQ=255)                                                                                agaagaCATCTGATGAGTAAGATTATCGCGACTTTGTATGTGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTg                                                          >  2:1280820/1‑150 (MQ=255)                                                                                agaagaCATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTg                                                          >  1:50593/1‑150 (MQ=255)                                                                                 gaagaCATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTgg                                                         <  1:1150416/150‑1 (MQ=255)                                                                                 gaagaCATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTgg                                                         >  2:383244/1‑150 (MQ=255)                                                                                  aagaCATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGa                                                        >  1:239224/1‑150 (MQ=255)                                                                                         cTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTgg                                                 >  2:1005482/1‑150 (MQ=255)                                                                                          tgatgaGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGc                                                <  1:14281/150‑1 (MQ=255)                                                                                                   aGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCcgc                                                                          >  2:519703/1‑115 (MQ=255)                                                                                                   aGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCcgc                                                                          <  1:519703/115‑1 (MQ=255)                                                                                                     aTTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTg                                     <  1:322198/150‑1 (MQ=255)                                                                                                          cgcgACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGAt                                <  1:314490/150‑1 (MQ=255)                                                                                                            cgACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGAtgt                              >  1:133716/1‑150 (MQ=255)                                                                                                               cTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTTGTAATCTGGCATGGTTTATTGCTGATTTggg                           <  2:1421441/150‑1 (MQ=255)                                                                                                                     aTGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCt                     >  1:387448/1‑150 (MQ=255)                                                                                                                       gCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCtgt                   <  2:794711/150‑1 (MQ=255)                                                                                                                        cGGTAATGGACAAGCGCCCCCGGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCtgtg                  >  2:427514/1‑150 (MQ=255)                                                                                                                         ggTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGc                 <  1:619202/150‑1 (MQ=255)                                                                                                                            aaTGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTgg              <  2:173802/150‑1 (MQ=255)                                                                                                                              aaggaaaGCGCCCGCAGGGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTggg                           <  1:1424176/130‑1 (MQ=255)                                                                                                                              aaggaaaGCGCCCGCAGGGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTggg                           >  2:1424176/6‑135 (MQ=255)                                                                                                                               ggACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGgtgt           <  2:239224/150‑1 (MQ=255)                                                                                                                                ggaaaGCGCCCGCAGGGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGGtgtg          >  1:315514/4‑150 (MQ=255)                                                                                                                                    aGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCAGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATAGCTGATGTGGGCCGTCTGTGCTGGTGTGATTc      >  1:551118/1‑150 (MQ=255)                                                                                                                                        cccccTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGGCTGTGCTGGTGTGATTCACgg  >  1:576720/1‑150 (MQ=255)                                                                                                                                             |                                                                                                                                                 TGGGAACGCTTCTTGCCTGTTCGTTTGGGGTAATCACCGCGCTGGCGCTTGAACACGTCGAATCAGGCAAAGCCGGTCAAGAAGACATCTGATGAGTAAGATTATCGCGACTTTGTATGCGGTAATGGACAAGCGCCCCCTGCGGGCGCTTTCCTTCGTGATGGCGCTTCTGTTAGCAGGATGTATGTTTTGGGACCCATCTCGTTTTGCCGCGAAGACCAGTGAACTGGAAATCTGGCATGGTTTATTGCTGATGTGGGCCGTCTGTGCTGGTGTGATTCACGG  >  NC_000913/774218‑774502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑