Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 786,614 T→G 6.3% C328G (TGC→GGC)  aroG → 3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913786,6140TG 6.3% 291.4 / 10.1 95C328G (TGC→GGC) aroG3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive
Reads supporting (aligned to +/- strand):  ref base T (46/43);  new base G (2/4);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

GATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCA  >  NC_000913/786470‑786763
                                                                                                                                                |                                                                                                                                                     
gatgTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATc                                                                                                                                                  <  1:1187997/150‑1 (MQ=255)
   gTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGc                                                                                                                                               >  2:810107/1‑150 (MQ=255)
     ttGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTg                                                                                                                                             >  1:63443/1‑150 (MQ=255)
     ttGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCAGGCATCggtga                                                                                                                                             <  2:179214/150‑4 (MQ=255)
     ttGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCAGGCATCggtga                                                                                                                                             <  1:1186722/150‑4 (MQ=255)
      tgtgCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTgg                                                                                                                                            >  2:1029371/1‑150 (MQ=255)
      tgtgCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCAGGCATCggtgat                                                                                                                                            <  2:640562/150‑5 (MQ=255)
        tgCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGGGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCTAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCAGGCATCggtgatgc                                                                                                                                          <  2:1269258/150‑7 (MQ=255)
         gCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATGGGCTGGGaa                                                                                                                                         <  2:50753/150‑1 (MQ=255)
         gCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGaa                                                                                                                                         >  1:114841/1‑150 (MQ=255)
          cTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTTGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAg                                                                                                                                        <  1:1045408/150‑1 (MQ=255)
          cTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAg                                                                                                                                        <  2:63443/150‑1 (MQ=255)
          cTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAg                                                                                                                                        >  1:1331939/1‑150 (MQ=255)
          cTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAg                                                                                                                                        <  1:158776/150‑1 (MQ=255)
            gACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGAt                                                                                                                                      <  2:521618/150‑1 (MQ=255)
                    ccAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGGCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGc                                                                                                                              >  1:615103/1‑150 (MQ=255)
                    ccAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGc                                                                                                                              >  1:725846/1‑150 (MQ=255)
                       gcagATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGctct                                                                                                                           >  1:1253637/1‑150 (MQ=255)
                             tGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACg                                                                                                                     <  1:1071132/150‑1 (MQ=255)
                              gCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGt                                                                                                                    >  2:1351986/1‑150 (MQ=255)
                               ccGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTc                                                                                                                   >  1:678116/1‑150 (MQ=255)
                                 ggTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCaa                                                                                                                 >  2:745864/1‑150 (MQ=255)
                                  gTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAAc                                                                                                                <  1:476199/150‑1 (MQ=255)
                                  gTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAAc                                                                                                                >  1:174619/1‑150 (MQ=255)
                                    ggCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTg                                                                                                              >  1:178970/1‑150 (MQ=255)
                                     gCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTgg                                                                                                             >  1:717242/1‑150 (MQ=255)
                                      tgAAAAGGACATTATTGGTGTGTTGGTGGTAAGCTATCTGGTTGATGTTAATCAGAGCCTCTAGAGCGGTGTTCCGTTGGCCAACGGTAAGAGCATCACCTATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAAGTGGc                                                                                                            <  2:610105/149‑1 (MQ=255)
                                      cGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGc                                                                                                            <  1:74269/150‑1 (MQ=255)
                                        aaaaGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGa                                                                                                          <  1:933212/150‑1 (MQ=255)
                                            ggCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGc                                                                                                      <  2:1028492/150‑1 (MQ=255)
                                              ccATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGc                                                                                                      >  2:1280271/1‑148 (MQ=255)
                                              ccATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGc                                                                                                      <  1:1280271/148‑1 (MQ=255)
                                              ccATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAg                                                                                                    >  2:1379501/1‑150 (MQ=255)
                                               cATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGt                                                                                                   >  2:907485/1‑150 (MQ=255)
                                                     tGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAgc                                                                                             >  1:699890/1‑150 (MQ=255)
                                                     tGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAgc                                                                                             <  2:1058049/150‑1 (MQ=255)
                                                     tGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAgc                                                                                             <  2:1275285/150‑1 (MQ=255)
                                                      ggCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACTGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAgcg                                                                                            <  2:619025/150‑1 (MQ=255)
                                                      ggCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAgcg                                                                                            >  1:347520/1‑150 (MQ=255)
                                                      ggCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAgcg                                                                                            <  2:347520/150‑1 (MQ=255)
                                                        cGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGcgcg                                                                                          >  2:669009/1‑150 (MQ=255)
                                                            aTGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTcgc                                                                                      >  1:40677/1‑150 (MQ=255)
                                                                 ggAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTa                                                                                 >  1:1371982/1‑150 (MQ=255)
                                                                   aaaGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAg                                                                               >  1:1281393/1‑150 (MQ=255)
                                                                       ccATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGttt                                                                           <  1:689408/150‑1 (MQ=255)
                                                                          tCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGTAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAAt                                                                        <  1:655458/150‑1 (MQ=255)
                                                                           cTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGc                                                                                                      >  1:1308107/1‑119 (MQ=255)
                                                                           cTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGc                                                                                                      <  2:1308107/119‑1 (MQ=255)
                                                                           cTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAAtt                                                                       >  1:368135/1‑150 (MQ=255)
                                                                            tggtggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTg                                                                      >  1:387131/1‑150 (MQ=255)
                                                                               tggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCg                                                                   >  1:310013/1‑150 (MQ=255)
                                                                               tggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCg                                                                   >  1:1096784/1‑150 (MQ=255)
                                                                               tggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCg                                                                   <  2:699890/150‑1 (MQ=255)
                                                                               tggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCg                                                                   <  2:923733/150‑1 (MQ=255)
                                                                                   aGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATg                                                               <  2:314273/150‑1 (MQ=255)
                                                                                    ggCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAATCGCGTAGCCGGTAAGGTTTAATTGTCTGATgc                                                              >  2:598953/1‑150 (MQ=255)
                                                                                       aaTCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGcc                                                           >  1:9858/1‑150 (MQ=255)
                                                                                        aTCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCg                                                          >  1:1385887/1‑150 (MQ=255)
                                                                                              gCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCagag                                                    >  2:502233/1‑150 (MQ=255)
                                                                                                  cGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGc                                                                                                      <  1:391286/96‑1 (MQ=255)
                                                                                                  cGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGc                                                                                                      >  2:391286/1‑96 (MQ=255)
                                                                                                  cGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGc                                                >  2:1224798/1‑150 (MQ=255)
                                                                                                   gagaGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCg                                               <  1:502233/150‑1 (MQ=255)
                                                                                                      cgCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTAt                                            <  1:369571/149‑1 (MQ=255)
                                                                                                       gCGGGGAGCCGCTGGCCTCCGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATc                                           <  1:292608/150‑1 (MQ=255)
                                                                                                        cGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATcc                                          <  1:567666/150‑1 (MQ=255)
                                                                                                           ggAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGAt                                       <  2:1331939/150‑1 (MQ=255)
                                                                                                            gAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATg                                      <  2:79146/150‑1 (MQ=255)
                                                                                                            gAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATg                                      >  2:1383754/1‑150 (MQ=255)
                                                                                                               ccGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAAt                                   <  1:550585/150‑1 (MQ=255)
                                                                                                                cGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATc                                  <  1:810107/150‑1 (MQ=255)
                                                                                                                 gCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATca                                 <  2:1253637/150‑1 (MQ=255)
                                                                                                                    ggCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCA                              <  2:9858‑M1/150‑1 (MQ=255)
                                                                                                                     gCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCAC                             >  2:1218703‑M1/1‑150 (MQ=255)
                                                                                                                        tACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAgg                          >  2:328510/1‑150 (MQ=255)
                                                                                                                        tACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAgg                          >  1:1311252/1‑150 (MQ=255)
                                                                                                                         aCGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGc                         >  2:819620/1‑150 (MQ=255)
                                                                                                                         aCGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGc                         <  1:1379501/150‑1 (MQ=255)
                                                                                                                             tAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGa                     <  2:286299/150‑1 (MQ=255)
                                                                                                                              aaGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCgg                                                                  <  2:44346/104‑1 (MQ=255)
                                                                                                                              aaGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCgg                                                                  >  1:44346/1‑104 (MQ=255)
                                                                                                                              aaGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGAt                    <  2:1281393/150‑1 (MQ=255)
                                                                                                                               agagCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATa                   <  1:598953/150‑1 (MQ=255)
                                                                                                                               agagCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATa                   <  2:678116/150‑1 (MQ=255)
                                                                                                                                gagCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATaa                  >  2:129141/1‑150 (MQ=255)
                                                                                                                                  gCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATgc                                                              >  2:902644/1‑104 (MQ=255)
                                                                                                                                  gCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATgc                                                              <  1:902644/104‑1 (MQ=255)
                                                                                                                                    aTCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTcg              <  1:669009/150‑1 (MQ=255)
                                                                                                                                     cagccGATGCAGGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTcgc             >  1:680075/4‑150 (MQ=255)
                                                                                                                                     cagccGATGCAGGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTcgc             >  1:1004939/4‑150 (MQ=255)
                                                                                                                                      cACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTcgcg            <  1:1132924/150‑1 (MQ=255)
                                                                                                                                        ccGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCa          >  1:449882/1‑150 (MQ=255)
                                                                                                                                        ccGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCa          >  1:445076/1‑150 (MQ=255)
                                                                                                                                             gCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTc     >  2:1062322/1‑150 (MQ=255)
                                                                                                                                               cTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGc   >  2:181132/1‑150 (MQ=255)
                                                                                                                                                tGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCa  <  1:907485/150‑1 (MQ=255)
                                                                                                                                                tGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCa  <  2:1415355/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCA  >  NC_000913/786470‑786763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑