Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,365,454 T→C 6.4% V408A (GTG→GCG)  puuB → gamma‑glutamylputrescine oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,365,4540TC 6.4% 184.6 / 3.2 62V408A (GTG→GCG) puuBgamma‑glutamylputrescine oxidase
Reads supporting (aligned to +/- strand):  ref base T (28/30);  new base C (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

TTACATGCAGGGCTACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTCCGCGCGGGGT  >  NC_000913/1365305‑1365602
                                                                                                                                                     |                                                                                                                                                    
ttACATGCAGGGCTACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCgtgt                                                                                                                                                      <  2:946367/150‑1 (MQ=255)
   cATGCAGGGCTACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGcc                                                                                                                                                   >  1:258171/1‑150 (MQ=255)
   cATGCAGGGCTACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGcc                                                                                                                                                   >  2:232876/1‑150 (MQ=255)
     tGCAGGGCTACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGt                                                                                                                                                 <  2:282004/150‑1 (MQ=255)
      gCAGGGCTACCGCGGCCACGGCGTGACCTGTACTCATCAAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGTTGCGTGTGCCGtt                                                                                                                                                <  2:244336/150‑1 (MQ=255)
      gCAGGGCTACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGtt                                                                                                                                                <  1:1323208/150‑1 (MQ=255)
       cAGGGCTACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGttt                                                                                                                                               >  2:719195/1‑150 (MQ=255)
         gggCTACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTAc                                                                                                                                             >  1:23373/1‑150 (MQ=255)
             tACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGtgtg                                                                                                                                                     >  2:712747/1‑138 (MQ=255)
             tACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGtgtg                                                                                                                                                     <  1:712747/138‑1 (MQ=255)
                   ggCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGgc                                                                                                                                   >  1:912936/1‑150 (MQ=255)
                      cACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGcgcg                                                                                                                                <  2:93085/150‑1 (MQ=255)
                        cGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCgcggc                                                                                                                              <  2:1258315/150‑1 (MQ=255)
                             tGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTAtt                                                                                                                         <  2:60999/150‑1 (MQ=255)
                                 cTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAg                                                                                                                     <  2:253733/150‑1 (MQ=255)
                                   gTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGcc                                                                                                                   <  1:737286/150‑1 (MQ=255)
                                   gTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGcc                                                                                                                   <  1:816215/150‑1 (MQ=255)
                                        cATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTgcgc                                                                                                              >  1:698918/1‑150 (MQ=255)
                                          tCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGa                                                                                                            >  1:390894/1‑150 (MQ=255)
                                          tCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGa                                                                                                            >  1:1113336/1‑150 (MQ=255)
                                           cTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCTATTCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGTCGTATTACAGCCTGCGCGAt                                                                                                           <  2:1067971/150‑1 (MQ=255)
                                             aGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGTCGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCg                                                                                                         <  1:1316127/150‑1 (MQ=255)
                                             aGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCg                                                                                                         <  1:232876/150‑1 (MQ=255)
                                             aGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCg                                                                                                         <  1:312602/150‑1 (MQ=255)
                                                cGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCt                                                                                                      >  2:183953/1‑150 (MQ=255)
                                                     gTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCg                                                                                                 >  2:338100/1‑150 (MQ=255)
                                                      tttGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGt                                                                                                >  1:62486/1‑150 (MQ=255)
                                                      tttGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGt                                                                                                >  2:5068/1‑150 (MQ=255)
                                                      tttGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGt                                                                                                >  2:347494/1‑150 (MQ=255)
                                                           ttGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAAt                                                                                           >  1:996861/1‑150 (MQ=255)
                                                                       tgcGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACcgg                                                                                                                                          <  1:208522/91‑2 (MQ=255)
                                                                       tgcGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACcgc                                                                                                                                          >  2:208522/1‑91 (MQ=255)
                                                                             gcgACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAag                                                                         <  2:825011/150‑1 (MQ=255)
                                                                                  gCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCa                                                                    >  2:219765/1‑150 (MQ=255)
                                                                                    cGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCaaa                                                                  >  1:239196/1‑150 (MQ=255)
                                                                                     gAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCaaaa                                                                 <  1:183953/150‑1 (MQ=255)
                                                                                       aCGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAgg                                                               <  2:23373/150‑1 (MQ=255)
                                                                                             cGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGaa                                                         <  2:698918/150‑1 (MQ=255)
                                                                                              gATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGaaa                                                        <  1:256530/150‑1 (MQ=255)
                                                                                                  ccTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATg                                                    <  2:964888/150‑1 (MQ=255)
                                                                                                      cGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCg                                                                                      >  2:229365/1‑112 (MQ=255)
                                                                                                      cGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCg                                                                                      <  1:229365/112‑1 (MQ=255)
                                                                                                      cGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGca                                                >  2:538458/1‑150 (MQ=255)
                                                                                                         cAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTaa                                                                                 >  1:833902/1‑114 (MQ=255)
                                                                                                         cAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTaa                                                                                 <  2:833902/114‑1 (MQ=255)
                                                                                                         cAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGcaaca                                             >  2:1230362/1‑150 (MQ=255)
                                                                                                         cAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGcaaca                                             >  1:1337470/1‑150 (MQ=255)
                                                                                                            tCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATg                                          <  2:390894/150‑1 (MQ=255)
                                                                                                             cTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGa                                         <  1:986538/150‑1 (MQ=255)
                                                                                                               gCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAAt                                       >  2:1404661/1‑150 (MQ=255)
                                                                                                                 cGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTc                                     <  1:888816/150‑1 (MQ=255)
                                                                                                                    aTTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCAt                                  >  1:943713/1‑150 (MQ=255)
                                                                                                                       aCCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAg                               >  2:418421/1‑150 (MQ=255)
                                                                                                                         ccGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGcg                             <  2:236417/150‑1 (MQ=255)
                                                                                                                                   aacggcaCACGCAGCGTGCGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCt                   >  1:1039174/4‑150 (MQ=255)
                                                                                                                                   aacggcaCACGCAGCGTGCGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCt                   >  1:1034174/4‑150 (MQ=255)
                                                                                                                                    cgggcgCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTg                  <  1:502643/150‑1 (MQ=255)
                                                                                                                                      ggcaCACGCAGCGTGCGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGcc                <  2:1039174/150‑1 (MQ=255)
                                                                                                                                      ggcaCACGCAGCGTGCGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGcc                <  2:1034174/150‑1 (MQ=255)
                                                                                                                                        cgcACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCAc              <  2:912936/150‑1 (MQ=255)
                                                                                                                                         gcACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACt             <  2:239196/150‑1 (MQ=255)
                                                                                                                                          cACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTc            >  1:499227/1‑150 (MQ=255)
                                                                                                                                            cGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTCcg          >  2:430698/1‑150 (MQ=255)
                                                                                                                                            cGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTCcg          >  2:1247020/1‑150 (MQ=255)
                                                                                                                                            cGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTCcg          <  1:5068/150‑1 (MQ=255)
                                                                                                                                                  gtgtGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTCCGCGCggg    <  1:930302/150‑1 (MQ=255)
                                                                                                                                                  gtgtGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTCCGCGCggg    <  1:219765/150‑1 (MQ=255)
                                                                                                                                                    gtgCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTCCGCGCGGGGt  <  1:1247020/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTACATGCAGGGCTACAGCGGCCACGGCGTGACCTGTACTCATCTAGCCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACCGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACTCCGCGCGGGGT  >  NC_000913/1365305‑1365602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑