BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,530,499	A→C	7.5%	I2M (ATT→ATG)	yncO ←	protein YncO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,530,499	0	A	C	7.5%	659.8 / 39.0	212	I2M (ATT→ATG)	yncO	protein YncO
Reads supporting (aligned to +/- strand): ref base A (83/113); new base C (4/12); total (87/125)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 219 total aligned reads displayed.

TTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGAAAG  >  NC_000913/1530350‑1530648

ttatGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTaa                                                                                                                                                       >  1:105898/1‑150 (MQ=255)

 tatGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTaaa                                                                                                                                                      >  2:795648/1‑150 (MQ=255)

   tGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATc                                                                                                                                                    >  1:1354208/1‑150 (MQ=255)

      tAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACg                                                                                                                                                 >  2:956353/1‑150 (MQ=255)

      tAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACg                                                                                                                                                 >  2:1312322/1‑150 (MQ=255)

      tAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACg                                                                                                                                                 >  1:877586/1‑150 (MQ=255)

         cAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGAtt                                                                                                                                              <  2:354868/150‑1 (MQ=255)

             ttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATTTACATCACAATTTTCa                                                                                                                                          <  2:573383/150‑1 (MQ=255)

             ttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCa                                                                                                                                          <  1:720873/150‑1 (MQ=255)

             ttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCa                                                                                                                                          <  2:497014/150‑1 (MQ=255)

             ttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTCa                                                                                                                                          <  2:1281993/150‑1 (MQ=255)

               gAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATc                                                                                                                                        <  2:1366575/150‑1 (MQ=255)

               gAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTCAtg                                                                                                                                        <  1:637098/150‑2 (MQ=255)

                aaaaaTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTCAtga                                                                                                                                       <  2:948760/150‑3 (MQ=255)

                   aaTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTg                                                                                                                                    <  1:568144/150‑1 (MQ=255)

                    aTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGc                                                                                                                                   <  2:916629/150‑1 (MQ=255)

                     tGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCt                                                                                                                                  <  1:855950/150‑1 (MQ=255)

                       aaGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCttt                                                                                                                                <  2:221646/150‑1 (MQ=255)

                        aGTTTTTGACTGGATTGGTAAATGGTCATAATGACCAACACCAGAAAGAGAAAAATATAAAATACAAACCGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTa                                                                                                                               <  2:919812/150‑1 (MQ=255)

                        aGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTa                                                                                                                               <  1:1188261/150‑1 (MQ=255)

                         gTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTaa                                                                                                                              >  1:196004/1‑150 (MQ=255)

                         gTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTaa                                                                                                                              >  1:1218930/1‑150 (MQ=255)

                             ttGACTGGATTGGTAAATGGTCATAATGAACAACACCACAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGCCCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGcc                                                                                                                          <  1:81774/150‑1 (MQ=255)

                              tGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGc                                                                                                                                   >  1:235468/1‑140 (MQ=255)

                              tGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGc                                                                                                                                   <  2:235468/140‑1 (MQ=255)

                                aCTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTAc                                                                                                                       <  1:1085448/150‑1 (MQ=255)

                                 cTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACa                                                                                                                      <  2:1218930/150‑1 (MQ=255)

                                 cTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACa                                                                                                                      <  1:598191/150‑1 (MQ=255)

                                    gATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGt                                                                                                                   <  2:258646/150‑1 (MQ=255)

                                       tGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGAt                                                                                                                <  2:134509/150‑1 (MQ=255)

                                        ggTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATc                                                                                                               <  2:1237105/150‑1 (MQ=255)

                                        ggTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATc                                                                                                               >  1:1203234/1‑150 (MQ=255)

                                              tGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAAtt                                                                                                         <  2:1091275/150‑1 (MQ=255)

                                               ggTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAAttt                                                                                                        <  2:1211212/150‑1 (MQ=255)

                                               ggTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAAttt                                                                                                        <  2:320730/150‑1 (MQ=255)

                                                gTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTa                                                                                                       >  1:1037943/1‑150 (MQ=255)

                                                    tAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATTTACATCACAATTTTc                                                                                                                                           <  2:1399733/110‑1 (MQ=255)

                                                      aTGAACAACACCAGAAAGAGAAAAATATAAAATACATACATAAAGACATGAGAGCAAGGGACGGGGCAGTGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCttt                                                                                                 <  1:951552/150‑1 (MQ=255)

                                                       tGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTg                                                                                                <  1:805178/150‑1 (MQ=255)

                                                       tGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTg                                                                                                <  2:196004/150‑1 (MQ=255)

                                                         aacaacACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGa                                                                                              <  2:311757/150‑1 (MQ=255)

                                                          acaacaCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGaa                                                                                             <  1:252653/150‑1 (MQ=255)

                                                          acaacaCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGaa                                                                                             >  1:1132743/1‑150 (MQ=255)

                                                            aacaCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATa                                                                                           <  2:653263/150‑1 (MQ=255)

                                                              cacCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAAc                                                                                         >  1:458398/1‑150 (MQ=255)

                                                               acCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACt                                                                                        <  2:864276/150‑1 (MQ=255)

                                                                   gAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTaaaa                                                                                    >  2:49915/1‑150 (MQ=255)

                                                                    aaaGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTaaaaa                                                                                   <  2:1043301/150‑1 (MQ=255)

                                                                         gaAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATaa                                                                              >  1:892824/1‑150 (MQ=255)

                                                                            aaaTATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATaaaaa                                                                           <  2:719922/150‑1 (MQ=255)

                                                                             aaTATAAAATAAATACAGAAAGACATGATACCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATaaaaaa                                                                          <  1:348395/150‑1 (MQ=255)

                                                                              atatAAAATACATACAGAAATAAATGAGAGCACTGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAg                                                                         <  1:533038/150‑1 (MQ=255)

                                                                              atatAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAg                                                                         <  1:1287001/150‑1 (MQ=255)

                                                                                 taAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAg                                                                         >  1:1411227/1‑147 (MQ=255)

                                                                                   aaaTACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGc                                                                    >  2:1174479/1‑150 (MQ=255)

                                                                                   aaaTACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGc                                                                    >  1:711111/1‑150 (MQ=255)

                                                                                         atacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTg                                                              <  2:1354208/150‑1 (MQ=255)

                                                                                           acaGAAAGACATGATAGCAAGGGACGGGGCATGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAg                                                            <  2:285343/150‑1 (MQ=255)

                                                                                            caGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAAc                                                                                         >  1:747442/1‑120 (MQ=255)

                                                                                            caGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGt                                                           >  2:152587/1‑150 (MQ=255)

                                                                                            caGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGt                                                           >  1:178766/1‑150 (MQ=255)

                                                                                              gAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTAt                                                         >  2:373101/1‑150 (MQ=255)

                                                                                               aaaGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTAtt                                                        <  1:274632/150‑1 (MQ=255)

                                                                                                 aGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCa                                                      >  1:1103962/1‑150 (MQ=255)

                                                                                                    cATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAAAGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAGAAAGGACGCCAGGTGAGTATTCAAAg                                                   <  2:1069697/150‑1 (MQ=255)

                                                                                                       gagaGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGttt                                                >  2:729938/1‑150 (MQ=255)

                                                                                                        agagCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTg                                               <  2:1233126/150‑1 (MQ=255)

                                                                                                         gagCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGc                                              >  1:1373768/1‑150 (MQ=255)

                                                                                                          agCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCt                                             >  1:677153/1‑150 (MQ=255)

                                                                                                              aGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGTAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGAt                                         <  1:1233932/150‑1 (MQ=255)

                                                                                                              aGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGAt                                         <  1:772324/150‑1 (MQ=255)

                                                                                                              aGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGATTGCTTGAt                                         >  2:1111997/1‑150 (MQ=255)

                                                                                                                ggACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAc                                                                                                     >  2:546963/1‑88 (MQ=255)

                                                                                                                ggACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATta                                       >  1:304332/1‑150 (MQ=255)

                                                                                                                     gggcagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCGTCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATAttt                                  >  2:507078/1‑150 (MQ=255)

                                                                                                                        cagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAg                               <  2:28308/150‑1 (MQ=255)

                                                                                                                        cagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAg                               <  2:411778/150‑1 (MQ=255)

                                                                                                                          gggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTg                             <  2:339981/150‑1 (MQ=255)

                                                                                                                          gggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTg                             <  2:892824/150‑1 (MQ=255)

                                                                                                                            gcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAc                           >  1:1385183/1‑150 (MQ=255)

                                                                                                                            gcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAc                           >  1:1245530/1‑150 (MQ=255)

                                                                                                                             caAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcc                          >  2:212339/1‑150 (MQ=255)

                                                                                                                             caAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcc                          >  1:926860/1‑150 (MQ=255)

                                                                                                                              aaGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAccc                         <  2:677153/150‑1 (MQ=255)

                                                                                                                               aGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcccc                        >  1:1423808/1‑150 (MQ=255)

                                                                                                                                gATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCt                       <  1:49915/150‑1 (MQ=255)

                                                                                                                                aaagaTGAAAATCGTGATTTACATCACAATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGATTGATTATATTTCAGTGACCCCt                       >  2:1427019/5‑150 (MQ=39)

                                                                                                                                   gaTGAAAATCGTGATTTACATCACAATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGat                    >  1:158429/2‑150 (MQ=255)

                                                                                                                                   cATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACTCCAGGTTAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGat                    >  2:814053/1‑150 (MQ=255)

                                                                                                                                     tGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGatat                  >  2:1008353/1‑150 (MQ=255)

                                                                                                                                      gAAAATCGTGATTTACATCACAATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGAtata                 >  2:854890/1‑150 (MQ=255)

                                                                                                                                        aaaTTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACg               <  2:877586/150‑1 (MQ=255)

                                                                                                                                          aTTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGac             <  1:795648/150‑1 (MQ=255)

                                                                                                                                          aTTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGac             <  1:174487/150‑1 (MQ=255)

                                                                                                                                           ttGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTTATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGaca            <  2:1109278/150‑1 (MQ=255)

                                                                                                                                           ttGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGaca            >  1:20604/1‑150 (MQ=255)

                                                                                                                                               gATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGAcaacaa        >  2:618271/1‑150 (MQ=255)

                                                                                                                                                aTGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAg       <  2:523400/150‑1 (MQ=255)

                                                                                                                                                   tAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGaa    >  2:1048710/1‑150 (MQ=255)

                                                                                                                                                     aaTCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGAAAg  <  2:1196728/150‑1 (MQ=255)

TTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGAAAG  >  NC_000913/1530350‑1530648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`