Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,567,466	G→C	6.5%	intergenic (‑247/+38)	yddY ← / ← yddW	protein YddY/putative lipoprotein YddW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,567,466	0	G	C	6.5%	282.5 / 7.6	89	intergenic (‑247/+38)	yddY/yddW	protein YddY/putative lipoprotein YddW
Reads supporting (aligned to +/- strand):  ref base G (38/45);  new base C (2/4);  total (40/49)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

ATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTA  >  NC_000913/1567317‑1567615                                                                                                                                                      |                                                                                                                                                      attattTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTTTTTAACTAAGTGTGGTTAATTTGACTTAAGTAATCATGATTATTAGTTGGATATTTTAATAGGTTAACAAGCCTGTgg                                                                                                                                                       <  2:1184472/150‑1 (MQ=255) attattTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTgg                                                                                                                                                       >  1:87414/1‑150 (MQ=255)  ttattCTTCGGTCACAGCGAATATCCTATCGATTTTCATATTCATACATTTTTATTAGGGATTTATCTCGGTTTAACTAACTCGGGTTACTTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGCGTAACACGCCGGTggg                                                                                                                                                      <  2:1281582/150‑1 (MQ=255)    attTTTCGGTGACAGCGAATATCGTATGGGTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTa                                                                                                                                                    <  2:1150644/150‑1 (MQ=255)      ttttCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTaaa                                                                                                                                                  >  2:77659/1‑150 (MQ=255)          ccgTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCAc                                                                                                                                              >  2:39033/3‑150 (MQ=255)             tGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCgg                                                                                                                                           <  2:680172/150‑1 (MQ=255)               aCAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCt                                                                                                                                         >  2:54112/1‑150 (MQ=255)               aCAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCt                                                                                                                                         <  1:932573/150‑1 (MQ=255)                cAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCtt                                                                                                                                        >  1:1170993/1‑150 (MQ=255)                  gCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGt                                                                                                                                      <  2:71097/150‑1 (MQ=255)                  gCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGt                                                                                                                                      <  1:937988/150‑1 (MQ=255)                  gCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCTTGt                                                                                                                                      <  1:335848/150‑1 (MQ=255)                   cGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCTTGtt                                                                                                                                     <  2:1172582/150‑1 (MQ=255)                    taaTATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGCGGGTAAAGCACCGGCTTGTTa                                                                                                                                    <  1:372587/149‑1 (MQ=255)                    gAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTa                                                                                                                                    <  2:1251144/150‑1 (MQ=255)                    gAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTa                                                                                                                                    <  2:154826/150‑1 (MQ=255)                     aaTATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTAc                                                                                                                                   >  1:664484/1‑150 (MQ=255)                       tatCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCTTGTTAccc                                                                                                                                 <  2:1247568/150‑3 (MQ=39)                        atCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                                <  2:752897/150‑1 (MQ=255)                           gTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTgg                                                                                                                                                       <  1:779889/124‑1 (MQ=255)                           gTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTgg                                                                                                                                                       >  2:779889/1‑124 (MQ=255)                           gTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTa                                                                                                                             >  2:744371/1‑150 (MQ=255)                           gTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTa                                                                                                                             <  1:124019/150‑1 (MQ=255)                            tATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTaa                                                                                                                            >  1:1407225/1‑150 (MQ=255)                             aTGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAg                                                                                                                           >  2:835101/1‑150 (MQ=255)                                gTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAAt                                                                                                                        >  2:504940/1‑150 (MQ=255)                                  ttttCATATTCATACATTTTTATTAGGGATTTTATGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                      >  2:244104/1‑150 (MQ=255)                                     tcatattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAg                                                                                                                           >  1:22091/1‑142 (MQ=255)                                     tcatattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAg                                                                                                                           <  2:22091/142‑1 (MQ=255)                                     tcatattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAg                                                                                                                   <  2:400930/150‑1 (MQ=255)                                        tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCTGTGGGTAAAGCACCGGCTTGTTACAAATTAAGAATGGGAGttt                                                                                                                <  2:564127/150‑1 (MQ=255)                                        tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGttt                                                                                                                >  2:933563/1‑150 (MQ=255)                                         attcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTa                                                                                                               <  1:1416166/150‑1 (MQ=255)                                           tcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAAc                                                                                                             <  2:664484/150‑1 (MQ=255)                                            cataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACt                                                                                                            >  1:1329961/1‑150 (MQ=255)                                            cataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACt                                                                                                            >  2:352993/1‑150 (MQ=255)                                             ataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTg                                                                                                           <  1:504940/150‑1 (MQ=255)                                              taCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGc                                                                                                          >  2:1248306/1‑150 (MQ=255)                                               aCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGcc                                                                                                         <  1:110435/150‑1 (MQ=255)                                               aCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGcc                                                                                                         <  1:1372128/150‑1 (MQ=255)                                                  tttttATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTATGAATGGGAGTTTAACTGCCCCa                                                                                                      >  2:906626/1‑150 (MQ=255)                                                  tttttATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCa                                                                                                      >  1:768140/1‑150 (MQ=255)                                                   ttttATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTA‑TAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGc                                                                                                    <  1:426125/150‑1 (MQ=255)                                                     ttattaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCg                                                                                                   <  2:1170993/150‑1 (MQ=255)                                                         tatggATTTATTGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCTGTGGGTAAAGCACCTGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACtt                                                                                               <  2:775210/147‑1 (MQ=255)                                                         taGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACtt                                                                                               <  1:630093/150‑1 (MQ=255)                                                          aGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACttt                                                                                              >  2:113725/1‑150 (MQ=255)                                                          aGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACttt                                                                                              >  1:714971/1‑150 (MQ=255)                                                           gggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTg                                                                                             >  2:28690/1‑150 (MQ=255)                                                             gATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCa                                                                                           >  2:813929/1‑150 (MQ=255)                                                             gATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCa                                                                                           <  1:994355/150‑1 (MQ=255)                                                                  aTGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAg                                                                                      <  2:1407225/150‑1 (MQ=255)                                                                   tGGCTGTTTAACTAAGTGTGTTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGc                                                                                     <  1:576740/150‑1 (MQ=255)                                                                   tGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGc                                                                                     <  2:406471/150‑1 (MQ=255)                                                                        gTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGAcc                                                                                <  1:1273919/150‑1 (MQ=255)                                                                           tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGTAGATAGCTGACCGCt                                                                             >  2:1318362/1‑150 (MQ=255)                                                                            aaCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                            <  2:87414/150‑1 (MQ=255)                                                                            aaCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                            >  2:209016/1‑150 (MQ=255)                                                                              cTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgt                                                                          >  2:1179110/1‑150 (MQ=255)                                                                                  gtgtGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAg                                                                      <  1:933563/150‑1 (MQ=255)                                                                                       gTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGc                                                                 >  2:142510/1‑150 (MQ=255)                                                                                        ttAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCg                                                                >  2:993033/1‑150 (MQ=255)                                                                                         tAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                               <  1:54112/150‑1 (MQ=255)                                                                                         tAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                               <  1:812049/150‑1 (MQ=255)                                                                                          aaTTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGt                                                              <  1:28690/150‑1 (MQ=255)                                                                                           aTTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGtt                                                             <  1:1383349/150‑1 (MQ=255)                                                                                           aTTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGtt                                                             <  2:145397/150‑1 (MQ=255)                                                                                               gACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTAtt                                                         >  2:480230/1‑150 (MQ=255)                                                                                                aCTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTc                                                        <  1:1029732/150‑1 (MQ=255)                                                                                                           cATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTc                                             >  1:1079373/1‑150 (MQ=255)                                                                                                             tGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCAc                                           >  2:1071288/1‑150 (MQ=255)                                                                                                             tGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCAc                                           <  2:1034408/150‑1 (MQ=255)                                                                                                               attattAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACgg                                         <  1:39033/150‑1 (MQ=255)                                                                                                                   ttaGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTACGGTTTATTCAGATAGTCCTCACGGAACa                                     >  1:1239077/1‑150 (MQ=255)                                                                                                                    taGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACaa                                    >  1:763166/1‑150 (MQ=255)                                                                                                                    taGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACaa                                    >  1:956977/1‑150 (MQ=255)                                                                                                                        gggATCGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATg                                <  2:881735/150‑1 (MQ=255)                                                                                                                             aGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGcc                           <  2:1006266/150‑1 (MQ=255)                                                                                                                                 tAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTa                       >  1:118008/1‑150 (MQ=255)                                                                                                                                   agagGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACg                                          >  2:173453/1‑129 (MQ=255)                                                                                                                                   agagGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACg                                          <  1:173453/129‑1 (MQ=255)                                                                                                                                    gagGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGACTGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAAtt                    <  2:142179/150‑1 (MQ=255)                                                                                                                                       ggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCg                 <  1:835101/150‑1 (MQ=255)                                                                                                                                       ggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCg                 >  2:924598/1‑150 (MQ=255)                                                                                                                                        gTAACAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCgg                >  1:736836/1‑150 (MQ=255)                                                                                                                                         tAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCggg               <  1:352993/150‑1 (MQ=255)                                                                                                                                         tAACAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCggg               >  1:1126331/1‑150 (MQ=255)                                                                                                                                         caaCAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGCTCAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAACATGGTGCCGCTAATTTCggg               <  2:16820/149‑1 (MQ=255)                                                                                                                                          aaCAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGc              <  2:935313/150‑1 (MQ=255)                                                                                                                                            cAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGcac            >  2:1273799/1‑150 (MQ=255)                                                                                                                                             aaGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGcaca           <  1:142510/150‑1 (MQ=255)                                                                                                                                              aGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAg          >  2:1103115/1‑150 (MQ=255)                                                                                                                                                 cGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCAt       >  2:1065204/1‑150 (MQ=255)                                                                                                                                                     gggTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCCCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGtt   <  2:1329961/150‑1 (MQ=255)                                                                                                                                                      ggTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGCTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTa  <  2:550732/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      ATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTA  >  NC_000913/1567317‑1567615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑