Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,720,365	T→A	8.1%	intergenic (+22/+25)	slyB → / ← slyA	outer membrane lipoprotein SlyB/DNA‑binding transcriptional dual regulator SlyA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,720,365	0	T	A	8.1%	184.6 / 5.8	62	intergenic (+22/+25)	slyB/slyA	outer membrane lipoprotein SlyB/DNA‑binding transcriptional dual regulator SlyA
Reads supporting (aligned to +/- strand):  ref base T (29/28);  new base A (2/3);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

TGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTT  >  NC_000913/1720216‑1720514                                                                                                                                                       |                                                                                                                                                      tGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGt                                                                                                                                                       >  1:234042/1‑150 (MQ=255)  ggAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGtt                                                                                                                                                      >  1:535748/1‑150 (MQ=255)  ggAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGt                                                                                                                                                       >  2:1414228/1‑149 (MQ=255)  ggAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGt                                                                                                                                                       <  1:1414228/149‑1 (MQ=255)      aTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                  <  1:613724/150‑1 (MQ=255)           ccaccatgATGGTATTACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcac                                                                                                                                             >  1:765362/8‑150 (MQ=255)             aaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCacac                                                                                                                                           <  1:961870/150‑1 (MQ=255)                aCGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGcc                                                                                                                                        <  2:827693/150‑1 (MQ=255)                   aTGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACCACACGcaaac                                                                                                                                     <  1:304763/150‑5 (MQ=255)                      gTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATCCCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCtac                                                                                                                                  >  2:1091736/1‑148 (MQ=255)                            ccATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCa                                                                                                                            >  1:135729/1‑150 (MQ=255)                             catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACCACACg                                                                                                                                          <  1:1402230/135‑1 (MQ=255)                             catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACCACACg                                                                                                                                          >  2:1402230/1‑135 (MQ=255)                                     gggtAGAGAAACTAGGCATGAGTCGTTTCTCTCCGGTCCAAC‑TGTTCGTACTGGCCAGCAATGGCAGTCAGGGCCCCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGGCAGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGc                                                                                                                   <  2:466057/149‑1 (MQ=255)                                          cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                              >  2:948408/1‑150 (MQ=255)                                          cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                              >  2:1015419/1‑150 (MQ=255)                                          atgAAACAAGGAAACACTGTTTTCTCTCGGGGCCAAC‑GTTTCGTATTGGCTTGCATTGGTTTTCAGGTGACGTTTTCTCCTCGCTAAATATGTTTGCGTGTGGTATGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTTGCCTGTa                                                                                                              <  2:694675/148‑1 (MQ=255)                                            gAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAAc                                                                                                            >  1:1147081/1‑150 (MQ=255)                                            gAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAAc                                                                                                            >  1:1430895/1‑150 (MQ=255)                                            gAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAAc                                                                                                            >  1:662786/1‑150 (MQ=255)                                              aaCAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTc                                                                                                          >  2:531519/1‑150 (MQ=255)                                                     cAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGata                                                                                                   >  1:1174154/1‑150 (MQ=255)                                                      aaCACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGatat                                                                                                  >  2:393484/1‑150 (MQ=255)                                                          gtCGTTTCTC‑CCGGGCCCACGGTGTGTTACTGGCCCCCATTGGCTGTCTGGTCACGTTTTCTCCGGTAAAAATATTTTTGGGTGTGGTCTGGTTCCTCACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATg                                                                                              <  2:135729/149‑1 (MQ=255)                                                          cTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATg                                                                                              >  1:1383998/1‑150 (MQ=255)                                                              tttCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACTCCCCCTGCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGATCa                                                                                          >  2:1228738/1‑150 (MQ=255)                                                                    tcCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                    >  1:7815/1‑150 (MQ=255)                                                                    tcCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCACCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                    >  1:1121365/1‑150 (MQ=255)                                                                      cGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                  <  1:393484/150‑1 (MQ=255)                                                                      cGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                  <  2:1147081/150‑1 (MQ=255)                                                                       gggCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATATGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCg                                                                                 <  2:535748/150‑1 (MQ=255)                                                                             aC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGc                                                                           >  1:270529/1‑150 (MQ=255)                                                                                gtgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGt                                                                         <  1:1015419/150‑1 (MQ=255)                                                                                 tgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                        >  1:60407/1‑150 (MQ=255)                                                                                 tgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                        >  1:1423946/1‑150 (MQ=255)                                                                                    cGTACTGGCCAGCAATGGCTTTCTGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATc                                                                     <  1:1077666/150‑1 (MQ=255)                                                                                    cGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATc                                                                     <  2:1347961/150‑1 (MQ=255)                                                                                        cTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGtt                                                                 >  1:1112434/1‑150 (MQ=255)                                                                                          ggCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGc                                                               >  2:1244787/1‑150 (MQ=255)                                                                                            ccAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                             >  2:91061/1‑150 (MQ=255)                                                                                            ccAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                             >  1:597171/1‑150 (MQ=255)                                                                                             cAGCAATGGCAGGCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTcc                                                            <  1:537003/150‑1 (MQ=255)                                                                                                 aaTGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGtt                                                        <  2:270529/150‑1 (MQ=255)                                                                                                     gCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCctc                                                    >  1:232086/1‑150 (MQ=255)                                                                                                         tCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCg                                                <  1:91061/150‑1 (MQ=255)                                                                                                          cAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCgg                                               <  2:234042/150‑1 (MQ=255)                                                                                                          cAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCgg                                               <  1:277854/150‑1 (MQ=255)                                                                                                             gTGACGGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTGCAGTTCCTCTGCGgaga                                            <  2:116919/150‑1 (MQ=255)                                                                                                              tGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGGGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGAt                                           <  1:225436/150‑1 (MQ=255)                                                                                                               gACCGTTTCTCCGCGCTAAAAAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATg                                          <  1:744360/150‑1 (MQ=255)                                                                                                                 ccGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGcc                                        >  1:951284/1‑150 (MQ=255)                                                                                                                   gTTTCTCCGCGCTAAATAAGTTTGGGTGGGGCCCGGTTAGTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAt                                      <  1:1091736/150‑1 (MQ=255)                                                                                                                     ttCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGt                                    <  2:662786/150‑1 (MQ=255)                                                                                                                        tcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAat                                 <  2:1430895/150‑1 (MQ=255)                                                                                                                         ccGCGCTAAATACGTTTGCGGGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAata                                <  1:514332/150‑1 (MQ=255)                                                                                                                               tAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCGGTTCCTCTGCGGAGATGCCATGTAATATTTCcg                          >  2:306645/1‑150 (MQ=255)                                                                                                                                aaaTAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgc                         >  2:258655/1‑150 (MQ=255)                                                                                                                                           cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt              >  1:194587/1‑150 (MQ=255)                                                                                                                                           cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt              <  1:1244787/150‑1 (MQ=255)                                                                                                                                           cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt              >  1:1186657/1‑150 (MQ=255)                                                                                                                                             tgtgGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTg                                                  <  2:928259/112‑1 (MQ=255)                                                                                                                                             tgtgGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTg                                                  >  1:928259/1‑112 (MQ=255)                                                                                                                                              gtgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTTTtaa           <  2:1112434/150‑1 (MQ=255)                                                                                                                                               tgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaat          >  1:855594/1‑150 (MQ=255)                                                                                                                                                   cAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACa      <  2:597171/150‑1 (MQ=255)                                                                                                                                                    aGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCCCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAg     <  2:894702/150‑1 (MQ=255)                                                                                                                                                       ttACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGATCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCtt  >  2:31307/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                      TGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTT  >  NC_000913/1720216‑1720514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑