Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,720,367	A→C	9.2%	intergenic (+24/+23)	slyB → / ← slyA	outer membrane lipoprotein SlyB/DNA‑binding transcriptional dual regulator SlyA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,720,367	0	A	C	9.2%	175.6 / 9.4	64	intergenic (+24/+23)	slyB/slyA	outer membrane lipoprotein SlyB/DNA‑binding transcriptional dual regulator SlyA
Reads supporting (aligned to +/- strand):  ref base A (31/27);  new base C (2/4);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

ATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTC  >  NC_000913/1720221‑1720515                                                                                                                                                    |                                                                                                                                                     aTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                   <  1:613724/150‑1 (MQ=255)      ccaccatgATGGTATTACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcac                                                                                                                                              >  1:765362/8‑150 (MQ=255)        aaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCacac                                                                                                                                            <  1:961870/150‑1 (MQ=255)           aCGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGcc                                                                                                                                         <  2:827693/150‑1 (MQ=255)              aTGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACCACACGcaaac                                                                                                                                      <  1:304763/150‑5 (MQ=255)                 gTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATCCCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCtac                                                                                                                                   >  2:1091736/1‑148 (MQ=255)                       ccATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCa                                                                                                                             >  1:135729/1‑150 (MQ=255)                        catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACCACACg                                                                                                                                           <  1:1402230/135‑1 (MQ=255)                        catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGTAACCTGACCACACg                                                                                                                                           >  2:1402230/1‑135 (MQ=255)                                gggtAGAGAAACTAGGCATGAGTCGTTTCTCTCCGGTCCAAC‑TGTTCGTACTGGCCAGCAATGGCAGTCAGGGCCCCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGGCAGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGc                                                                                                                    <  2:466057/149‑1 (MQ=255)                                     cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                               >  2:948408/1‑150 (MQ=255)                                     cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                               >  2:1015419/1‑150 (MQ=255)                                     atgAAACAAGGAAACACTGTTTTCTCTCGGGGCCAAC‑GTTTCGTATTGGCTTGCATTGGTTTTCAGGTGACGTTTTCTCCTCGCTAAATATGTTTGCGTGTGGTATGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTTGCCTGTa                                                                                                               <  2:694675/148‑1 (MQ=255)                                       gAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAAc                                                                                                             >  1:1147081/1‑150 (MQ=255)                                       gAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAAc                                                                                                             >  1:662786/1‑150 (MQ=255)                                       gAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAAc                                                                                                             >  1:1430895/1‑150 (MQ=255)                                         aaCAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTc                                                                                                           >  2:531519/1‑150 (MQ=255)                                                cAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGata                                                                                                    >  1:1174154/1‑150 (MQ=255)                                                 aaCACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGatat                                                                                                   >  2:393484/1‑150 (MQ=255)                                                     gtCGTTTCTC‑CCGGGCCCACGGTGTGTTACTGGCCCCCATTGGCTGTCTGGTCACGTTTTCTCCGGTAAAAATATTTTTGGGTGTGGTCTGGTTCCTCACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATg                                                                                               <  2:135729/149‑1 (MQ=255)                                                     cTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATg                                                                                               >  1:1383998/1‑150 (MQ=255)                                                         tttCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACTCCCCCTGCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGATCa                                                                                           >  2:1228738/1‑150 (MQ=255)                                                               tcCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                     >  1:7815/1‑150 (MQ=255)                                                               tcCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCACCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                     >  1:1121365/1‑150 (MQ=255)                                                                 cGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                   <  1:393484/150‑1 (MQ=255)                                                                 cGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                   <  2:1147081/150‑1 (MQ=255)                                                                  gggCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATATGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCg                                                                                  <  2:535748/150‑1 (MQ=255)                                                                        aC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGc                                                                            >  1:270529/1‑150 (MQ=255)                                                                           gtgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGt                                                                          <  1:1015419/150‑1 (MQ=255)                                                                            tgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                         >  1:60407/1‑150 (MQ=255)                                                                            tgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                         >  1:1423946/1‑150 (MQ=255)                                                                               cGTACTGGCCAGCAATGGCTTTCTGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATc                                                                      <  1:1077666/150‑1 (MQ=255)                                                                               cGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATc                                                                      <  2:1347961/150‑1 (MQ=255)                                                                                   cTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGtt                                                                  >  1:1112434/1‑150 (MQ=255)                                                                                     ggCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGc                                                                >  2:1244787/1‑150 (MQ=255)                                                                                       ccAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                              >  2:91061/1‑150 (MQ=255)                                                                                       ccAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                              >  1:597171/1‑150 (MQ=255)                                                                                        cAGCAATGGCAGGCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTcc                                                             <  1:537003/150‑1 (MQ=255)                                                                                            aaTGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGtt                                                         <  2:270529/150‑1 (MQ=255)                                                                                                gCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCctc                                                     >  1:232086/1‑150 (MQ=255)                                                                                                    tCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCg                                                 <  1:91061/150‑1 (MQ=255)                                                                                                     cAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCgg                                                <  1:277854/150‑1 (MQ=255)                                                                                                     cAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCgg                                                <  2:234042/150‑1 (MQ=255)                                                                                                        gTGACGGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTGCAGTTCCTCTGCGgaga                                             <  2:116919/150‑1 (MQ=255)                                                                                                         tGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGGGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGAt                                            <  1:225436/150‑1 (MQ=255)                                                                                                          gACCGTTTCTCCGCGCTAAAAAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATg                                           <  1:744360/150‑1 (MQ=255)                                                                                                            ccGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGcc                                         >  1:951284/1‑150 (MQ=255)                                                                                                              gTTTCTCCGCGCTAAATAAGTTTGGGTGGGGCCCGGTTAGTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAt                                       <  1:1091736/150‑1 (MQ=255)                                                                                                                ttCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGt                                     <  2:662786/150‑1 (MQ=255)                                                                                                                   tcCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAat                                  <  2:1430895/150‑1 (MQ=255)                                                                                                                    ccGCGCTAAATACGTTTGCGGGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAata                                 <  1:514332/150‑1 (MQ=255)                                                                                                                          tAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCGGTTCCTCTGCGGAGATGCCATGTAATATTTCcg                           >  2:306645/1‑150 (MQ=255)                                                                                                                           aaaTAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgc                          >  2:258655/1‑150 (MQ=255)                                                                                                                                      cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt               >  1:194587/1‑150 (MQ=255)                                                                                                                                      cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt               <  1:1244787/150‑1 (MQ=255)                                                                                                                                      cGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt               >  1:1186657/1‑150 (MQ=255)                                                                                                                                        tgtgGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTg                                                   >  1:928259/1‑112 (MQ=255)                                                                                                                                        tgtgGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTg                                                   <  2:928259/112‑1 (MQ=255)                                                                                                                                         gtgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTTTtaa            <  2:1112434/150‑1 (MQ=255)                                                                                                                                          tgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaat           >  1:855594/1‑150 (MQ=255)                                                                                                                                              cAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACa       <  2:597171/150‑1 (MQ=255)                                                                                                                                               aGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCCCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAg      <  2:894702/150‑1 (MQ=255)                                                                                                                                                  ttACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGATCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCtt   >  2:31307/1‑150 (MQ=255)                                                                                                                                                   tACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTc  >  2:298851/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                     ATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAC‑GTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCTTC  >  NC_000913/1720221‑1720515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑